In Vitro Models of Cardiovascular Disease: Embryoid Bodies, Organoids and Everything in Between DOI Creative Commons
Theodora M. Stougiannou, Konstantinos Christodoulou, Dimos Karangelis

et al.

Biomedicines, Journal Year: 2024, Volume and Issue: 12(12), P. 2714 - 2714

Published: Nov. 27, 2024

Cardiovascular disease comprises a group of disorders affecting or originating within tissues and organs the cardiovascular system; most, if not all, will eventually result in cardiomyocyte dysfunction death, negatively impacting cardiac function. Effective models are thus important for understanding crucial aspects progression, while recent advancements stem cell biology have allowed use populations to derive such models. These include three-dimensional (3D) as cell-based embryos (SCME) well organoids, many which frequently derived from embryoid bodies (EB). Not only can they recapitulate 3D form function, but developmental programs governing self-organization into more complex well. Many different organoids SCME constructs been generated years recreate tissue that give rise its cellular composition unique morphology. It is purpose this narrative literature review describe summarize recently organoid their recapitulation genetic acquired disease. Owing examined, focus on injury associated with embryonic/fetal tissues.

Language: Английский

Organ-on-a-chip: quo vademus? Applications and regulatory status DOI Creative Commons
Maria Mendes, Ana Sofia Morais,

A Carlos

et al.

Colloids and Surfaces B Biointerfaces, Journal Year: 2025, Volume and Issue: 249, P. 114507 - 114507

Published: Jan. 8, 2025

Organ-on-a-chip systems, also referred to as microphysiological systems (MPS), represent an advance in bioengineering microsystems designed mimic key aspects of human organ physiology and function. Drawing inspiration from the intricate hierarchical architecture body, these innovative platforms have emerged invaluable vitro tools with wide-ranging applications drug discovery development, well enhancing our understanding disease physiology. The facility replicate tissues within physiologically relevant three-dimensional multicellular environments empowers organ-on-a-chip versatility throughout different stages development process. Moreover, can be tailored specific states, facilitating investigation progression, responses, potential therapeutic interventions. In particular, they demonstrate, early-phase pre-clinical studies, safety toxicity profiles compounds. Furthermore, play a pivotal role evaluation efficacy modeling diseases. One most promising prospects technology is simulate pathophysiology subpopulations even individual patients, thereby being used personalized medicine. By mimicking physiological responses diverse patient groups, hold promise revolutionizing strategies, guiding them towards intervention unique needs each patient. This review presents status evolution microfluidic that facilitated transition cells organs recreated on chips some opportunities offered by technology. Additionally, current future perspectives challenges this still faces are discussed.

Language: Английский

Citations

1
Advances in induced pluripotent stem cell‐derived cardiac myocytes: technological breakthroughs, key discoveries and new applications DOI Creative Commons
Colleen E. Clancy, Luis F. Santana

The Journal of Physiology, Journal Year: 2024, Volume and Issue: 602(16), P. 3871 - 3892

Published: July 20, 2024

Abstract A transformation is underway in precision and patient‐specific medicine. Rapid progress has been enabled by multiple new technologies including induced pluripotent stem cell‐derived cardiac myocytes (iPSC‐CMs). Here, we delve into these advancements their future promise, focusing on the efficiency of reprogramming techniques, fidelity differentiation lineage, functional characterization resulting myocytes, many applications silico models to understand general mechanisms controlling excitation–contraction coupling health disease. Furthermore, explore current potential iPSC‐CMs both research clinical settings, underscoring far‐reaching implications this rapidly evolving field. image

Language: Английский

Citations

5
From injury to repair: the therapeutic potential of induced pluripotent stem cells in heart failure DOI Creative Commons
Tadahisa Sugiura, Sheeza Nawaz, Dhienda C. Shahannaz

et al.

Regenerative medicine reports ., Journal Year: 2025, Volume and Issue: 2(1), P. 22 - 30

Published: March 1, 2025

Heart failure is a complex clinical syndrome characterized by the heart’s inability to pump sufficient blood meet body’s metabolic demands, resulting in symptoms such as dyspnea, fatigue, and fluid retention. Despite significant advances pharmacological treatments device therapies, prognosis for patients with advanced heart remains poor. This underscores urgent need innovative regenerative therapies aimed at restoring cardiac function improving quality of life suffering from this debilitating condition. article provides comprehensive overview pathophysiologic mechanisms underlying end-stage failure, which include myocardial systolic diastolic dysfunction, neuroendocrine activation, inflammation, oxidative stress. Collectively, these lead progressive deterioration structure function, culminating failure. The also examines differences between ischemic non-ischemic cardiomyopathies their implications treatment strategies prognosis. Furthermore, review explores application medicine focusing on its potential repair functional recovery. In field medicine, induced pluripotent stem cells derived cardiomyocytes are considered cutting-edge technologies repair. Induced can differentiate into cardiomyocytes, thereby offering new hope regeneration. However, current research faces several challenges, including maturity integration immunogenicity concerns, difficulties scaling up production applications. Additionally, ethical issues related cell technology, sourcing tumorigenic risks, must be carefully addressed. highlights technology treating while emphasizing complexities involved transitioning laboratory practice.

Language: Английский

Citations

0
Advances in humanoid organoid-based research on inter-organ communications during cardiac organogenesis and cardiovascular diseases DOI Creative Commons

Baoqiang Ni,

Lingqun Ye, Yan Zhang

et al.

Journal of Translational Medicine, Journal Year: 2025, Volume and Issue: 23(1)

Published: March 28, 2025

The intimate correlation between cardiovascular diseases and other organ pathologies, such as metabolic kidney diseases, underscores the intricate interactions among these organs. Understanding inter-organ communications is crucial for developing more precise drugs effective treatments systemic diseases. While animal models have traditionally been pivotal in studying interactions, human-induced pluripotent stem cells (hiPSCs) offer distinct advantages when constructing vitro models. Beyond conventional two-dimensional co-culture model, hiPSC-derived humanoid organoids emerged a substantial advancement, capable of replicating essential structural functional attributes internal organs vitro. This breakthrough has spurred development multilineage organoids, assembloids, organoids-on-a-chip technologies, which allow enhanced physiological relevance. These technologies shown great potential mimicking coordinated organogenesis, exploring disease pathogenesis, facilitating drug discovery. As central system, heart serves focal point an extensively studied network interactions. review focuses on advancements challenges organs, presenting comprehensive exploration this cutting-edge approach research.

Language: Английский

Citations

0
Construction of Engineered Cardiac Tissue on a Heart-on-a-Chip Device Enables Modeling of Arrhythmogenic Right Ventricular Cardiomyopathy DOI

Kai-Yun Qu,

Hongyi Cheng, Qiao Li

et al.

Biosensors and Bioelectronics, Journal Year: 2025, Volume and Issue: unknown, P. 117478 - 117478

Published: April 1, 2025

Language: Английский

Citations

0
Perspectives on organ-on-a-chip technology for natural products evaluation DOI Creative Commons
Xin Wang, Yu‐Hang Miao, Xiaomin Zhao

et al.

Food & medicine homology., Journal Year: 2024, Volume and Issue: 1(2), P. 9420013 - 9420013

Published: Aug. 7, 2024

Natural products have always been a treasure trove for clinical drug development and source of inspiration lead compounds in the process new discovery. However, two-dimensional cell cultures animal models traditional model serious limitations generalizing human physiopathology cannot accurately predict real response body to drugs, which brings obstacles challenges evaluation. Organ-on-a-chip (OoC) is an emerging technology based on microfluidic platforms in vitro culture that can mimic physiological environment function organs disease modeling In this review, we explore several major examples how single-OoC systems be used simulate complex outline recent advances organoids natural screening. Finally, summarize future trends OoCs must overcome discovery development. Overall, review highlights OoCs, instead models, open avenues evaluation, therapeutic innovation, vivo embodiment personalized medicine.

Language: Английский

Citations

2
In Vitro Models of Cardiovascular Disease: Embryoid Bodies, Organoids and Everything in Between DOI Creative Commons
Theodora M. Stougiannou, Konstantinos Christodoulou, Dimos Karangelis

et al.

Biomedicines, Journal Year: 2024, Volume and Issue: 12(12), P. 2714 - 2714

Published: Nov. 27, 2024

Cardiovascular disease comprises a group of disorders affecting or originating within tissues and organs the cardiovascular system; most, if not all, will eventually result in cardiomyocyte dysfunction death, negatively impacting cardiac function. Effective models are thus important for understanding crucial aspects progression, while recent advancements stem cell biology have allowed use populations to derive such models. These include three-dimensional (3D) as cell-based embryos (SCME) well organoids, many which frequently derived from embryoid bodies (EB). Not only can they recapitulate 3D form function, but developmental programs governing self-organization into more complex well. Many different organoids SCME constructs been generated years recreate tissue that give rise its cellular composition unique morphology. It is purpose this narrative literature review describe summarize recently organoid their recapitulation genetic acquired disease. Owing examined, focus on injury associated with embryonic/fetal tissues.

Language: Английский

Citations

0