Identification, functional characterization and expression profiling of three triterpene synthases from the legume plant Vigna unguiculata. DOI
Panayiota Markou, Constantine Garagounis,

Dionysia A. Fasoula

et al.

Biochemical and Biophysical Research Communications, Journal Year: 2024, Volume and Issue: 738, P. 150935 - 150935

Published: Nov. 1, 2024

Language: Английский

Controllable skeletal reorganizations in natural product synthesis DOI Creative Commons
Zeliang Zhang, Qian Xiao, Yu‐Cheng Gu

et al.

Natural Product Reports, Journal Year: 2024, Volume and Issue: 41(2), P. 251 - 272

Published: Jan. 1, 2024

Covering: 2016 to 2023The synthetic chemistry community is always in pursuit of efficient routes natural products. Among the many available general strategies, skeletal reorganization, which involves formation, cleavage, and migration C-C C-heteroatom bonds, stands out as a particularly useful approach for assembly molecular skeletons. In addition, it allows late-stage modification products quick access other family members or unnatural derivatives. This review summarizes syntheses steroid, terpenoid, alkaloid that have been achieved by means this strategy past eight years. Our goal illustrate strategy's potency reveal spectacular human ingenuity demonstrated its use development.

Language: Английский

Citations

10

Exploring and expanding the natural chemical space of bacterial diterpenes DOI Creative Commons

Xiuting Wei,

Wenbo Ning, Caitlin A. McCadden

et al.

Nature Communications, Journal Year: 2025, Volume and Issue: 16(1)

Published: April 19, 2025

Terpenoids are the largest family of natural products but relatively rare in bacteria. Genome mining reveals widespread prevalence terpene synthases, enzymes responsible for constructing hydrocarbon skeletons, Here, we show that 125 (37%) 334 synthases from 8 phyla, 17 classes, and 83 genera bacteria active as diterpene synthases. Isolation structural elucidation 28 diterpenes 31 reveal three previously unreported skeletons other organisms with unknown biosynthetic pathways, known to best our knowledge not seen bacteria, or new stereochemical isomers diterpenes. We also identify type I myxobacteria cyanobacteria. This study will help discover products, advance studies terpenoid biosynthesis enzymology, provide model systems probe ecological roles terpenes.

Language: Английский

Citations

1

Cloning and functional characterization of sesquiterpene synthase genes from Inonotus obliquus using a Saccharomyces cerevisiae expression system DOI
Qiao Liu, Jinwu Peng, Tao Zhang

et al.

World Journal of Microbiology and Biotechnology, Journal Year: 2025, Volume and Issue: 41(2)

Published: Jan. 30, 2025

Language: Английский

Citations

1

Overexpression of tRNA m7G modification methyltransferase complex promotes the biosynthesis of triterpene in yeast DOI Creative Commons

Mengyu Ma,

Jun Wang, Zhengwei Tan

et al.

Frontiers in Microbiology, Journal Year: 2025, Volume and Issue: 16

Published: March 31, 2025

The sustainable production of valuable compounds using microbial cell factories is an effective approach, yet further metabolic engineering strategies are needed to enhance their biosynthetic potential. Recent studies suggest that RNA modifications can influence cellular metabolism, but role in remains largely unexplored. squalene and lupeol different yeast strains was detected by gas chromatography-mass spectrometry (GC-MS) equipment. Transcriptomic analysis performed identify changes associated with the epigenetic modification. transcriptional translational expression targeted genes were determined real-time quantitative polymerase chain reaction western blotting, respectively. mRNA stability measured decay assay. In this study, overexpression Trm8 Trm82 complex, mediating tRNA 7-methylguanosine (m7G) modification yeast, significantly increased CEN.PK2-1C. Transcriptome indicated Trm8/Trm82 upregulated levels involved amino acid synthesis, glycolysis, tricarboxylic cycle, enhanced upstream acetyl-CoA biosynthesis, might be responsible for promoted biosynthesis squalene. Further investigation demonstrated complex could increase engineered yeast. These findings indicate m7G regulate central metabolism terpenoid biosynthesis. This study provides new insights into as a potential strategy improving high-value compounds.

Language: Английский

Citations

0

Vanadium haloperoxidases as noncanonical terpene synthases DOI
Jackson T. Baumgartner,

Lia I. Lozano Salazar,

Lukas A. Varga

et al.

Methods in enzymology on CD-ROM/Methods in enzymology, Journal Year: 2024, Volume and Issue: unknown, P. 447 - 475

Published: Jan. 1, 2024

Language: Английский

Citations

3

Early-Stage Biosynthesis of Phenalinolactone Diterpenoids Involves Sequential Prenylation, Epoxidation, and Cyclization DOI Creative Commons
Tyler A. Alsup, Zining Li, Caitlin A. McCadden

et al.

RSC Chemical Biology, Journal Year: 2024, Volume and Issue: 5(10), P. 1010 - 1016

Published: Jan. 1, 2024

The chemical logic associated with assembly of many bacterial terpenoids remains poorly understood. We focused our efforts on the early-stage biosynthesis phenalinolactone diterpenoids, demonstrating that

Language: Английский

Citations

3

Spokewise Total Syntheses of Four Erythrina Alkaloids and Telescoped Syntheses of Six Additional Alkaloids DOI

Yue‐Hong Hu,

Zhang‐Yan Gan,

Qin-Tong Li

et al.

The Journal of Organic Chemistry, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 18, 2024

Based on rich sulfur-involving chemical transformations, a novel spokewise synthetic strategy, subclass of the collective strategies, has been developed to concisely synthesize four erythrina alkaloids through single-step transformation from common precursor. Moreover, six additional have also synthesized by subsequent 1-2 steps transformations. The current approaches provide valuable platform for total syntheses and

Language: Английский

Citations

3

The role and mechanisms of canonical and non-canonical tailoring enzymes in bacterial terpenoid biosynthesis DOI Creative Commons
Yuya Kakumu,

Ayesha Ahmed Chaudhri,

Eric J. N. Helfrich

et al.

Natural Product Reports, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

This review explores recent insights into the roles and enzymatic mechanisms of canonical non-canonical tailoring enzymes in shaping modifying terpene hydrocarbon scaffolds during bacterial biosynthesis.

Language: Английский

Citations

0

The molecular complexity of terpene biosynthesis in red algae: current state and future perspectives DOI Creative Commons
Wanessa Francesconi Stida, Renato Crespo Pereira,

Esthfanny dos Santos Souza Azevedo

et al.

Natural Product Reports, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

In this article, we review the key processes contributing to chemical diversity of terpenes in red algae. We present knowledge gaps and perspectives for heterologous biosynthesis.

Language: Английский

Citations

0

Bioinformatics assisted construction of the link between biosynthetic gene clusters and secondary metabolites in fungi DOI
Huawei Lv,

Jia-Gui Tang,

Bin Wei

et al.

Biotechnology Advances, Journal Year: 2025, Volume and Issue: unknown, P. 108547 - 108547

Published: Feb. 1, 2025

Language: Английский

Citations

0