The Analyst, Journal Year: 2024, Volume and Issue: unknown
Published: Nov. 14, 2024
Schematic diagram of the strategies used for LTP ECL.
Language: Английский
Angewandte Chemie International Edition, Journal Year: 2024, Volume and Issue: 63(29)
Published: May 14, 2024
Electrochemiluminescence (ECL) is rapidly evolving from an analytical method into optical microscopy. The orthogonality of the electrochemical trigger and readout distinguishes it classic microscopy techniques, owing to its near-zero background, remarkable sensitivity, absence photobleaching phototoxicity. In this minireview, we summarize recent advances in ECL imaging technology, emphasizing original configurations which enable biological entities improvement properties by increasing complexity multiplexing bioassays. Additionally, mapping (electro)chemical reactivity space provides valuable information on nanomaterials facilitates deciphering mechanisms for improving their performances diagnostics (electro)catalysis. Finally, highlight achievements at ultimate limits single molecules, photons or chemical reactions, current challenges translate other fields such as material science, catalysis biology.
Language: Английский
Citations
26
Accounts of Chemical Research, Journal Year: 2024, Volume and Issue: 57(15), P. 2144 - 2153
Published: July 17, 2024
ConspectusElectrochemiluminescence (ECL) is the electrochemical generation of light. It involves an interfacial charge transfer that produces excited state a luminophore at electrode surface. ECL powerful readout method widely employed for immunoassays and clinical diagnostics progressively evolving into microscopy technique. On other hand, photoelectrochemistry illuminated semiconductors field research deals with photogenerated carriers solid-liquid interface. This concept offers several advantages such as considerable lowering onset potential required triggering reaction well light addressable chemistry, via spatial confinement redox reactions locally semiconductor electrodes. The combination termed photoinduced (PECL). through minority solid/liquid PECL results in conversion incident photons (λ
Language: Английский
Citations
7
Chemical Science, Journal Year: 2024, Volume and Issue: unknown
Published: Jan. 1, 2024
The successful integration of the SPECM measurement technique to provide deeper insights into underlying mechanisms responsible for improved ECL performance is reported.
Language: Английский
Citations
6
ChemBioChem, Journal Year: 2024, Volume and Issue: unknown
Published: Aug. 2, 2024
Electrochemiluminescence (ECL) is one of the most powerful techniques that meet needs analysis and detection in a variety scenarios, because its highly analytical sensitivity excellent spatiotemporal controllability. ECL combined with microscopy (ECLM) offers promising approach for quantifying mapping wide range analytes. To date, ECLM has been widely used to image biological entities processes, such as cells, subcellular structures, proteins membrane transport properties. In this review, we first introduced mechanisms several classic systems, then highlighted progress visual biosensing bioimaging by last decade. Finally, characteristics were summarized, well some current challenges. The future research interests potential directions application also outlooked.
Language: Английский
Citations
4
Analytical Chemistry, Journal Year: 2024, Volume and Issue: 96(45), P. 18262 - 18268
Published: Nov. 1, 2024
Optimization of electrochemiluminescence (ECL) immunoassays is highly beneficial for enhancing clinical diagnostics. A major challenge the improvement operation conditions required bead-based using typical [Ru(bpy)3]2+/tri-n-propylamine (TPrA) system. In this study, we report a heterogeneous immunoassay based on near-infrared photoinduced ECL, which facilitates imaging and quantitative analysis [Ru(bpy)3]2+-modified immunobeads at low anodic potential. The photovoltage generated by photoanode under light promotes oxidation processes electrode/electrolyte interface, thus considerably lowering onset potential both TPrA ECL emission. anti-Stokes shift between excitation (invisible to human eyes) visible emitted results in clear stable signal from immunobeads. addition, it offers possibility site-selective photoexcitation process. This approach not only meets performance traditional accuracy but also additional benefits lower requirements enhanced stability, providing new perspective optimization commercial immunoassays.
Language: Английский
Citations
4
ACS Applied Materials & Interfaces, Journal Year: 2024, Volume and Issue: 16(9), P. 11722 - 11729
Published: Feb. 23, 2024
Infrared (IR) imaging devices that convert IR irradiation (invisible to the human eye) a visible signal are based on solid-state components. Here, we introduce an alternative concept light-addressable electrochemistry (i.e., spatially confined under action of light stimulus) involves use liquid electrolyte. In this method, projection near-IR image (λexc = 850 or 840 nm) onto photoactive Si-based photoanode, immersed into phase, triggers locally photoinduced electrochemiluminescence (PECL) efficient [Ru(bpy)3]2+-TPrA system. This leads local conversion (λPECL 632 light. We demonstrate compared planar Si photoanodes, micropillar array large enhancement generation and considerably improves resolution PECL by preventing photogenerated minority carriers from diffusing laterally. These results important for design original can lead applications in photothermal analytical chemistry.
Language: Английский
Citations
3
Advanced Functional Materials, Journal Year: 2025, Volume and Issue: unknown
Published: March 12, 2025
Abstract Electrochemical technology provides unique opportunities to create an environment‐friendly and sustainable chemical industry. Nano‐electrochemistry offers valuable insights into the comprehension of electrochemical mechanisms behaviors at nanoscale, as opposed complex ensemble systems. Despite use various analytical tools, such nanopore scanning probe microscopy, for nano‐electrochemical research, direct operando high‐throughput spatially resolved measurements remain challenging. Electrochemiluminescence (ECL) is reactions‐dependent luminescence process without external light sources. Coupled with a microscopy configuration, novel imaging methodology ECL (ECLM), developed. ECLM, due its distinctive mode, holds considerable potential in field nano‐electrochemistry. This review will initially present overview basic principles ECL. The next section describe design ECLM apparatus discuss diverse applications Finally, challenges outline prospects further development technique summarized.
Language: Английский
Citations
0
Analytical Chemistry, Journal Year: 2024, Volume and Issue: 96(28), P. 11146 - 11154
Published: June 25, 2024
Here, we report an electrochemiluminescence (ECL)-based approach for imaging of local photoelectrochemical processes on hematite in a spatially and temporally controlled manner. The were guided by flexible dynamic light illumination, not requiring any prepatterned conductive features or photomasks, with digital micromirror device (DMD). was based light-addressable electrochemical reactions hematite, resulting photoinduced ECL emission spatiotemporally resolved selectively illumination. After clarifying the capability as photosensitive electrode, validated that illuminated exhibited stable light-guided correspondence area, spatial resolution 0.8 μm temporal 1 μs, even over long period 6 h. More importantly, this study exemplified simple yet effective ECL-based visualization adjustment illumination way.
Language: Английский
Citations
2
Angewandte Chemie, Journal Year: 2024, Volume and Issue: 136(29)
Published: May 14, 2024
Abstract Electrochemiluminescence (ECL) is rapidly evolving from an analytical method into optical microscopy. The orthogonality of the electrochemical trigger and readout distinguishes it classic microscopy techniques, owing to its near‐zero background, remarkable sensitivity, absence photobleaching phototoxicity. In this minireview, we summarize recent advances in ECL imaging technology, emphasizing original configurations which enable biological entities improvement properties by increasing complexity multiplexing bioassays. Additionally, mapping (electro)chemical reactivity space provides valuable information on nanomaterials facilitates deciphering mechanisms for improving their performances diagnostics (electro)catalysis. Finally, highlight achievements at ultimate limits single molecules, photons or chemical reactions, current challenges translate other fields such as material science, catalysis biology.
Language: Английский
Citations
0
The Analyst, Journal Year: 2024, Volume and Issue: unknown
Published: Nov. 14, 2024
Schematic diagram of the strategies used for LTP ECL.
Language: Английский
Citations
0