Decoding the Promiscuous Activity of Bile Salt Hydrolase DOI Open Access
Munishwar N. Gupta, Vladimir N. Uversky

Qeios, Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 29, 2024

The recently identified bile salt hydrolase (BSH) from gastrointestinal bacteria catalyzes the formation of bacterial acid amidates (BBAAs), significantly impacting host metabolism. While this activity was characterized as promiscuous, underlying mechanism not explored. This commentary proposes that BSH exhibits condition promiscuity, where typical hydrolytic enzymes catalyze synthetic reactions under specific conditions. Drawing parallels with micellar enzymology, we suggest salts, acting both substrates and micelle-forming agents, create an environment conducive for to amidation. represents a potential first in vivo demonstration such mechanism. Future investigations should explore BSH-catalyzed salts below critical micelle concentrations alternative surfactants validate hypothesis.

Language: Английский

Biological importance of arginine: A comprehensive review of the roles in structure, disorder, and functionality of peptides and proteins DOI
Munishwar N. Gupta, Vladimir N. Uversky

International Journal of Biological Macromolecules, Journal Year: 2023, Volume and Issue: 257, P. 128646 - 128646

Published: Dec. 6, 2023

Language: Английский

Citations

33

Sustainable dressings for wound healing DOI Creative Commons
Munishwar N. Gupta,

Avinash Rangaraju,

Premlata Ambre

et al.

Deleted Journal, Journal Year: 2025, Volume and Issue: 2(1)

Published: Jan. 2, 2025

Abstract The field of chronic wound care has been rapidly evolving. With a market size for wounds in billions USD, this is not an insignificant area to encourage more sustainable practices and processes. sourcing starting materials dressings from renewable resources the first crucial step ensuring that environmental impact minimized. Next important choice select methodologies accordance with principles green chemistry. This involves utilizing solvents as reaction media chemical processes preparing dressing materials. In many cases, like production hydrogels, crosslinking reagents are often employed. These can be, general, easily replaced by enzymes peroxidases, tyrosinases sortases. Process intensification strategies have emerged powerful approach ensure economy sustainability at large scale. Use microwave radiations ultrasonication instead heat accelerate rates helps cases inject wounds. review attempts highlight ongoing efforts future possibilities development cost-effective methods. Graphical

Language: Английский

Citations

1

Protein structure–function continuum model: Emerging nexuses between specificity, evolution, and structure DOI
Munishwar N. Gupta, Vladimir N. Uversky

Protein Science, Journal Year: 2024, Volume and Issue: 33(4)

Published: March 27, 2024

Abstract The rationale for replacing the old binary of structure–function with trinity structure, disorder, and function has gained considerable ground in recent years. A continuum model based on expanded form existing paradigm can now subsume importance both conformational flexibility intrinsic disorder protein function. is actually critical understanding protein–protein interactions many regulatory processes, formation membrane‐less organelles, our revised notions specificity as amply illustrated by moonlighting proteins. While its amyloids prions often discussed, roles infectious diseases under extreme conditions are also becoming clear. This review an attempt to discuss how current function, specificity, evolution fit better model. integration structure a single may bring greater clarity continuing quest proteins molecular mechanisms their functionality.

Language: Английский

Citations

6

Decoding the Promiscuous Activity of Bile Salt Hydrolase DOI Creative Commons
Munishwar N. Gupta, Vladimir N. Uversky

Published: Aug. 5, 2024

The recently identified bile salt hydrolase (BSH) from gastrointestinal bacteria catalyzes the formation of bacterial acid amidates (BBAAs), significantly impacting host metabolism. While this activity was characterized as promiscuous, underlying mechanism not explored. This commentary proposes that BSH exhibits condition promiscuity, where typical hydrolytic enzymes catalyze synthetic reactions under specific conditions. Drawing parallels with micellar enzymology, we suggest salts, acting both substrates and micelle-forming agents, create an environment conducive for to amidation. represents a potential first in vivo demonstration such mechanism. Future investigations should explore BSH-catalyzed salts below critical micelle concentrations alternative surfactants validate hypothesis.

Language: Английский

Citations

0

Decoding the Promiscuous Activity of Bile Salt Hydrolase DOI Open Access
Munishwar N. Gupta, Vladimir N. Uversky

Qeios, Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 29, 2024

The recently identified bile salt hydrolase (BSH) from gastrointestinal bacteria catalyzes the formation of bacterial acid amidates (BBAAs), significantly impacting host metabolism. While this activity was characterized as promiscuous, underlying mechanism not explored. This commentary proposes that BSH exhibits condition promiscuity, where typical hydrolytic enzymes catalyze synthetic reactions under specific conditions. Drawing parallels with micellar enzymology, we suggest salts, acting both substrates and micelle-forming agents, create an environment conducive for to amidation. represents a potential first in vivo demonstration such mechanism. Future investigations should explore BSH-catalyzed salts below critical micelle concentrations alternative surfactants validate hypothesis.

Language: Английский

Citations

0