Mitigating Photoaged Skin Aging with Single Injection of Recombinant Human Collagen Type Iii DOI
Yafang Chen, Kang Li, Hu Chen

et al.

Published: Jan. 1, 2023

Delaying aging is an eternal goal for humanity. Collagen-based materials, employed as dermal fillers to smoothen wrinkles, have attained extensive utilization. Nevertheless, traditional animal-derived collagen protein primarily presents concerns pertaining disease risks, potential immunological reactions, and batch instability. In this investigation, the filler composed of recombinant human type III (rhCol III) with excellent injectability was fabricated using synthetic biology. The in vitro examination cell interaction rhCol revealed commendable biocompatibility, concentration-dependent impacts on proliferation, migration secretion. Subsequently, we established a skin damage animal model through UV-photoaging introduced bioactive material vivo implantation, systematically comparing its biological effect pCollagen, hyaluronic acid (HA) saline thorough histological observation, immunofluorescence staining, hydroxyproline quantification analysis specific gene expression. Outcomes indicated prompted augmented production I (Col I), elastic fibers, thereby contributing remodeling extracellular matrix (ECM). summary, our investigation contributed robust biosafety proficient attenuation UV-induced photoaging by under single injection. Despite imperative ongoing efforts required successful translation from bench clinic, discernibly superior safety efficacy profile positioned it promising candidate within domain fillers.

Language: Английский

Chitosan-based injectable hydrogel with multifunction for wound healing: A critical review DOI

Aiqin Li,

Bin Ma,

Shiyao Hua

et al.

Carbohydrate Polymers, Journal Year: 2024, Volume and Issue: 333, P. 121952 - 121952

Published: Feb. 19, 2024

Language: Английский

Citations

43
Advancements in silk fibroin and silk sericin-based biomaterial applications for cancer therapy and wound dressing formulation: A comprehensive review DOI
Mohamed A. Hassan,

Amal A. Basha,

Mohamed Eraky

et al.

International Journal of Pharmaceutics, Journal Year: 2024, Volume and Issue: 662, P. 124494 - 124494

Published: July 20, 2024

Language: Английский

Citations

14
Stepwise co-assembled biomimetic hydrogel with antibacterial, angiogenesis, and tissue regeneration acceleration for diabetic wound healing DOI Creative Commons
Tingting Wang, Xiaomin Li, Siqi Liu

et al.

Materials & Design, Journal Year: 2024, Volume and Issue: 244, P. 113149 - 113149

Published: July 8, 2024

Bacterial infections and inadequate tissue regeneration capacity are prevalent issues in diabetic wounds, impeding the healing process. To solve these problems, a biomimetic hydrogel combined with bacterial cellulose (BC), recombinant human collagen type III (RHC), ε-poly-L-Lysine (EPL) is fabricated by stepwise co-assembly technology. Owing to RHC EPL modifications of BC, exhibited antibacterial effects promoted fibroblast proliferation angiogenesis, which accelerated wounds. Biomimetic hydrogels exhibit good mechanical properties excellent water absorption, that can protect wounds provide moist environment. In addition, vitro studies have shown activity biocompatibility. vivo on wound promote accelerate deposition, re-epithelialize sites healing. This study provides new promising strategy for treatment

Language: Английский

Citations

4
Hydrogen sulfide releasing poly(γ-glutamic acid) biocomposite hydrogel with monitoring, antioxidant, and antibacterial properties for diabetic wound healing DOI
Xu Liu, Xiao Han,

Yushuang Shang

et al.

International Journal of Biological Macromolecules, Journal Year: 2023, Volume and Issue: 253, P. 127053 - 127053

Published: Sept. 28, 2023

Language: Английский

Citations

10
Advancements in Clinical Utilization of Recombinant Human Collagen: An Extensive Review DOI Creative Commons

Isaac Wong Kai Jie,

Kar Wai Alvin Lee, So Jeong Yoon

et al.

Life, Journal Year: 2025, Volume and Issue: 15(4), P. 582 - 582

Published: April 1, 2025

Introduction: Recombinant human collagen, developed through advanced recombinant DNA technology, has emerged as a cutting-edge biomaterial with diverse applications in medicine. It addresses significant limitations of animal-derived collagens, such immunogenicity and the risk zoonotic diseases. Objective: This review evaluates clinical applications, benefits, challenges associated focusing on its potential to transform medical surgical practices. Methods: A comprehensive search was conducted MEDLINE, PubMed, Ovid databases using keywords “Recombinant Human Collagen”, “Collagen-Based Biomaterials”, “Clinical Applications”, “Tissue Repair”, “Wound Healing”. Relevant studies, including trials diagnostic were analyzed classified according Oxford Centre for Evidence-Based Medicine evidence hierarchy. Findings: collagen demonstrates superior mechanical properties controlled degradation rates compared traditional sources. Clinical studies highlight effectiveness accelerating wound closure, promoting dermal regeneration, minimizing scarring, making it particularly valuable chronic management interventions. In tissue engineering, scaffolds have shown regenerating cartilage, bone, cardiovascular tissues by supporting cell proliferation, differentiation, matrix deposition. Additionally, adaptability forming hydrogels matrices enhances suitability drug delivery systems, enabling sustained release therapeutic agents. Conclusion: represents transformative advancement practice, providing safer more effective alternative Its demonstrated success healing, highlights significantly improve patient outcomes. However, high production costs, regulatory complexities, long-term biocompatibility remain barriers widespread adoption. Further research collaboration between biotechnology developers authorities are essential fully realize potential.

Language: Английский

Citations

0
Light-controlled crosslinked multifunctional "Band-Aids" as dual-stage wound dressing for dynamic wound closure DOI Creative Commons
Xinyue Zhang, Zhan Xue, Chen Hu

et al.

Deleted Journal, Journal Year: 2024, Volume and Issue: 6(1)

Published: June 1, 2024

Abstract The objective of regenerative wound healing dressings is to accelerate skin tissue regeneration and restore normal physiological function at sites. Achieving this goal requires biomaterials capable repairing distinct phases in a way that balances material function, degradation, safety, growth. In study, we introduced novel dual-stage dressing system comprising methacrylic anhydride-modified recombinant humanized type III collagen (rhCol III-MA) dopamine (DMA) (RMDM), which was synthesized through free radical polymerization π-π stacking. Within system, RMDM formulated into two forms with identical compositions: hydrogel sponge, tailored for application across various stages repair. These materials displayed favorable hemocompatibility, biocompatibility, antioxidant properties, angiogenic potential vitro . Moreover, the vivo experiments also demonstrated sponges could rapidly stop bleeding wounds mouse tail amputation liver incision models. Notably, sponge/gel (S/G) accelerated compared individual sponge gel treatments rat full-thickness model, underscoring synergistic benefits combining repair different stages. Therefore, research provides valuable insights designing advanced can be specific healing, may have significant biomedical applications. Graphical

Language: Английский

Citations

3
Injectable Biomimetic Gels for Biomedical Applications DOI Creative Commons
Hossein Omidian,

Renae L. Wilson,

Sumana Dey Chowdhury

et al.

Biomimetics, Journal Year: 2024, Volume and Issue: 9(7), P. 418 - 418

Published: July 8, 2024

Biomimetic gels are synthetic materials designed to mimic the properties and functions of natural biological systems, such as tissues cellular environments. This manuscript explores advancements future directions injectable biomimetic in biomedical applications highlights significant potential hydrogels wound healing, tissue regeneration, controlled drug delivery due their enhanced biocompatibility, multifunctionality, mechanical properties. Despite these advancements, challenges resilience, degradation rates, scalable manufacturing remain. discusses ongoing research optimize properties, develop cost-effective production techniques, integrate emerging technologies like 3D bioprinting nanotechnology. Addressing through collaborative efforts is essential for unlocking full engineering regenerative medicine.

Language: Английский

Citations

3
Recent advances of collagen composite biomaterials for biomedical engineering: antibacterial functionalization and 3D-printed architecturalization DOI Creative Commons

Lu Zheng,

Natalya Tseomashko,

Anastasiia Voronova

et al.

Deleted Journal, Journal Year: 2024, Volume and Issue: 6(1)

Published: May 22, 2024

Abstract Collagen possesses high biocompatibility with all tissue and cell types in the body, enabling creation of multifunctional composite materials for medical applications. In biomedical engineering, naturally-sourced collagen is often combined diverse organic inorganic bioactive components to eliminate defects disorders fields including orthopedics, dermatology, more. At same time, medical-related infection issues precise treatment needs patients require biomaterials have antibacterial properties customized structures. This paper reviews functionalization recent years, combination or agents, which beneficial preventing controlling biological contamination Then, existing problems future development directions architecturalization 3D printing were discussed, providing guidance personalized customization meet specific future. Graphical

Language: Английский

Citations

2
Advanced Wound Healing and Scar Reduction Using an Innovative Anti-ROS Polysaccharide Hydrogel with Recombinant Human Collagen Type III DOI
Xinyue Zhang, Yawen Huang, Tao Luo

et al.

ACS Applied Materials & Interfaces, Journal Year: 2024, Volume and Issue: 16(38), P. 50305 - 50320

Published: Sept. 10, 2024

Excessive fibrotic scar formation during skin defect repair poses a formidable challenge, impeding the simultaneous acceleration of wound healing and prevention hindering restoration integrity functionality. Drawing inspiration from structural, compositional, biological attributes skin, we developed hydrogel containing modified recombinant human collagen type III thiolated hyaluronic acid to address challenges regenerating appendages improving recovery functions after injury by reducing scarring. The displayed favorable biocompatibility, antioxidant properties, angiogenic potential, fibroblast migration stimulation

Language: Английский

Citations

2
Design and evaluation of a bilayered dermal/hypodermal 3D model using a biomimetic hydrogel formulation DOI Creative Commons
Carlos Chocarro‐Wrona, Julia López de Andrés, Pablo Rioboó‐Legaspi

et al.

Biomedicine & Pharmacotherapy, Journal Year: 2024, Volume and Issue: 177, P. 117051 - 117051

Published: July 2, 2024

Due to the limitations of current skin wound treatments, it is highly valuable have a healing formulation that mimics extracellular matrix (ECM) and mechanical properties natural tissue. Here, novel biomimetic hydrogel has been developed based on mixture Agarose-Collagen Type I (AC) combined with ECM-related components: Dermatan sulfate (DS), Hyaluronic acid (HA), Elastin (EL) for its application in tissue engineering (TE). Different formulations were designed by combining AC hydrogels DS, HA, EL. Cell viability, hemocompatibility, physicochemical, mechanical, investigated. Finally, bilayered loaded fibroblasts mesenchymal stromal cells was using Ag-Col I-DS-HA-EL (ACDHE) formulation. The ACDHE displayed best vitro results acceptable physicochemical properties. Also, behaved mechanically close human native exhibited good cytocompatibility. Environmental scanning electron microscopy (ESEM) analysis revealed porous microstructure allows maintenance cell growth ECM-like structure production. These findings demonstrate potential applications such as an injectable or bioink create cell-laden structures TE.

Language: Английский

Citations

1