Confined biofabrication in inflatable bioreactor: toward the sterile production of implantable tissues and organs DOI Creative Commons
Alexandre Dufour, Lucie Essayan,

Céline Thomann

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Feb. 26, 2024

Abstract The future of organ and tissue biofabrication strongly relies on 3D bioprinting technologies. However, maintaining sterility remains a critical issue regardless the technology used. This challenge becomes even more pronounced when volume bioprinted objects approaches dimensions. Here, we introduce novel device called Flexible Unique Generator Unit (FUGU), which is unique combination flexible silicone membranes solid components made printed medical-grade polycarbonate. FUGU designed to support micro-extrusion needle insertion removal, internal adjustment, fluid management. was tested in various environments, ranging from custom-built basic cartesian sophisticated 6-axis robotic arm bioprinters, demonstrating its compatibility, flexibility, universality across different platforms. Sterility assays conducted under infection scenarios highlight FUGU’s ability physically protect against contaminations, thereby ensuring integrity constructs. also enabled cultivation 14.5 cm³ human colorectal cancer model within completely confined sterile environment, while allowing for exchange gases with external environment. system represents significant advancement biofabrication, paving path toward production implantable tissues organs.

Language: Английский

Cultivation of Bovine Mesenchymal Stem Cells on Plant-Based Scaffolds in a Macrofluidic Single-Use Bioreactor for Cultured Meat DOI Creative Commons
Gilad Gome,

Benyamin Chak,

Shadi Tawil

et al.

Foods, Journal Year: 2024, Volume and Issue: 13(9), P. 1361 - 1361

Published: April 28, 2024

Reducing production costs, known as scaling, is a significant obstacle in the advancement of cultivated meat. The cultivation process hinges on several key components, e.g., cells, media, scaffolds, and bioreactors. This study demonstrates an innovative approach, departing from traditional stainless steel or glass bioreactors, by integrating food-grade plant-based scaffolds thermoplastic film While films are commonly used for constructing fluidic systems, conventional welding methods cost-prohibitive lack rapid prototyping capabilities, thus inflating research development expenses. developed laser technique facilitates contamination-free leakproof sealing polyethylene films, enabling efficient fabrication macrofluidic systems with various designs dimensions. By incorporating such rice seeded bovine mesenchymal stem into these this sterile cell proliferation within systems. approach not only reduces bioreactor construction costs but also addresses need scalable solutions both industrial settings. Integrating single-use bioreactors minimal shear forces macro carriers puffed may further enhance biomass scaled-out model. use aligns sustainable practices tissue engineering cultured-meat production, emphasizing its suitability diverse applications.

Language: Английский

Citations

11
Hydrogel-Based 3D Bioprinting Technology for Articular Cartilage Regenerative Engineering DOI Creative Commons
Hongji Zhang,

Zheyuan Zhou,

Fengjie Zhang

et al.

Gels, Journal Year: 2024, Volume and Issue: 10(7), P. 430 - 430

Published: June 28, 2024

Articular cartilage is an avascular tissue with very limited capacity of self-regeneration. Trauma or injury-related defects, inflammation, aging in articular can induce progressive degenerative joint diseases such as osteoarthritis. There are significant clinical demands for the development effective therapeutic approaches to promote repair regeneration. The current treatment modalities used lesions mainly include cell-based therapy, small molecules, surgical approaches, and engineering. However, these remain unsatisfactory. With advent three-dimensional (3D) bioprinting technology, engineering provides opportunity defects degeneration through construction organized, living structures composed biomaterials, chondrogenic cells, bioactive factors. bioprinted cartilage-like mimic native cartilage, opposed traditional by allowing excellent control cell distribution modulation biomechanical biochemical properties high precision. This review focuses on various hydrogels, including natural synthetic their developments bioinks 3D In addition, challenges prospects hydrogels applications also discussed.

Language: Английский

Citations

5
Confined bioprinting and culture in inflatable bioreactor for the sterile bioproduction of tissues and organs DOI Creative Commons
Alexandre Dufour, Lucie Essayan,

Céline Thomann

et al.

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: May 14, 2024

The future of organ and tissue biofabrication strongly relies on 3D bioprinting technologies. However, maintaining sterility remains a critical issue regardless the technology used. This challenge becomes even more pronounced when volume bioprinted objects approaches dimensions. Here, we introduce novel device called Flexible Unique Generator Unit (FUGU), which is unique combination flexible silicone membranes solid components made stainless steel. Alternatively, can also be printed medical-grade polycarbonate. FUGU designed to support micro-extrusion needle insertion removal, internal adjustment, fluid management. was assessed in various environments, ranging from custom-built basic cartesian sophisticated 6-axis robotic arm bioprinters, demonstrating its compatibility, flexibility, universality across different platforms. Sterility assays conducted under infection scenarios highlight FUGU's ability physically protect against contaminations, thereby ensuring integrity constructs. enabled cultivation 14.5 cm

Language: Английский

Citations

4
Physical stimuli-responsive 3D printable hydrogels for scalable manufacturing of bioengineered meat analogs DOI
Jieun Lee, Sayan Deb Dutta, Tejal V. Patil

et al.

Trends in Food Science & Technology, Journal Year: 2025, Volume and Issue: unknown, P. 104867 - 104867

Published: Jan. 1, 2025

Language: Английский

Citations

0
3D (Bio) Printing Combined Fiber Fabrication Methods for Tissue Engineering Applications: Possibilities and Limitations DOI Creative Commons
Waseem Kitana, Indra Apsite, Leonid Ionov

et al.

Advanced Functional Materials, Journal Year: 2025, Volume and Issue: unknown

Published: April 14, 2025

Abstract Biofabrication is an emerging interdisciplinary field of engineering that aims to develop technologies for applications in tissue and regenerative medicine. A progressing biofabrication technology 3D (bio) printing (3DBP), which allows controlled spatial deposition cell‐laden bioinks a layer‐by‐layer approach fabricate biologically active constructs. Although 3DBP can create some relevant structures, it uses hydrogels, are isotropic nature do not provide sufficient mechanical properties reconstruct many tissues, such as cartilage, bone, skin. Additionally, hydrogels alone replicate the complex hierarchical buildup native extracellular matrix (ECM), contains both gel‐like fibrous components. Replicating tissue's structure mechanically by incorporating fibers would result enhanced biological performance. This possible integrating fiber fabrication techniques. Thus, harnessing strengths each technique eliminating their limitations. will enable hybrid constructs with multiscale hierarchy performance comparable tissue. review highlight attempts combine methods applications. different techniques discussed, showcasing limitations integration 3DBP.

Language: Английский

Citations

0
Mechanical Stimulation and Aligned Poly(ε-caprolactone)–Gelatin Electrospun Scaffolds Promote Skeletal Muscle Regeneration DOI Creative Commons
Francisco José Calero-Castro, Víctor Manuel Pérez Puyana, Imán Laga

et al.

ACS Applied Bio Materials, Journal Year: 2024, Volume and Issue: 7(10), P. 6430 - 6440

Published: Oct. 4, 2024

The current treatments to restore skeletal muscle defects present several injuries. creation of scaffolds and implant that allow the regeneration this tissue is a solution reaching researchers' interest. To achieve this, electrospinning useful technique manufacture with nanofibers different orientation. In work, polycaprolactone gelatin solutions were tested fabricate electrospun two degrees alignment between their fibers: random aligned. These can be seeded myoblast C2C12 then stimulated mechanical bioreactor mimics physiological conditions tissue. Cell viability as well cytoskeletal morphology functionality was measured. Myotubes in aligned (9.84 ± 1.15 μm) thinner than (11.55 3.39 μm;

Language: Английский

Citations

3
Soft bioreactor systems: a necessary step toward engineered MSK soft tissue? DOI Creative Commons
Nicole Dvorak, Zekun Liu, Pierre‐Alexis Mouthuy

et al.

Frontiers in Robotics and AI, Journal Year: 2024, Volume and Issue: 11

Published: April 22, 2024

A key objective of tissue engineering (TE) is to produce in vitro funcional grafts that can replace damaged tissues or organs patients. TE uses bioreactors, which are controlled environments, allowing the application physical and biochemical cues relevant cells growing biomaterials. For soft musculoskeletal (MSK) such as tendons, ligaments cartilage, it now well established applied mechanical stresses be incorporated into those bioreactor systems support growth maturation via activation mechanotransduction pathways. However, laboratory often oversimplified compared found physiologically may a factor slow progression engineered MSK towards clinic. In recent years, an increasing number studies have focused on complex loading conditions, applying different types direction constructs, order better mimic cellular environment experienced vivo . Such highlighted need improve upon traditional rigid limited uniaxial loading, apply multiaxial elucidate their influence maturation. To address this need, bioreactors emerged. They employ one more components, flexible chambers twist bend with actuation, compliant actuators construct, sensors record measurements situ This review examines shortcomings, highlights advances TE. Challenges future applications discussed, drawing attention exciting prospect these platforms ability aid development functional grafts.

Language: Английский

Citations

2
Confined biofabrication in inflatable bioreactor: toward the sterile production of implantable tissues and organs DOI Creative Commons
Alexandre Dufour, Lucie Essayan,

Céline Thomann

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Feb. 26, 2024

Abstract The future of organ and tissue biofabrication strongly relies on 3D bioprinting technologies. However, maintaining sterility remains a critical issue regardless the technology used. This challenge becomes even more pronounced when volume bioprinted objects approaches dimensions. Here, we introduce novel device called Flexible Unique Generator Unit (FUGU), which is unique combination flexible silicone membranes solid components made printed medical-grade polycarbonate. FUGU designed to support micro-extrusion needle insertion removal, internal adjustment, fluid management. was tested in various environments, ranging from custom-built basic cartesian sophisticated 6-axis robotic arm bioprinters, demonstrating its compatibility, flexibility, universality across different platforms. Sterility assays conducted under infection scenarios highlight FUGU’s ability physically protect against contaminations, thereby ensuring integrity constructs. also enabled cultivation 14.5 cm³ human colorectal cancer model within completely confined sterile environment, while allowing for exchange gases with external environment. system represents significant advancement biofabrication, paving path toward production implantable tissues organs.

Language: Английский

Citations

0