Self-Responsive Biomimetic Short Lipopeptide-Based Delivery Systems for Enhanced Antibiotic Efficacy against Drug-Resistant Infections

RSC Medicinal Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Lipopeptide-inspired, short peptide amphiphile-meropenem nanostructures enhance antibiotic efficacy, increase local concentration, reduce dosages, and minimize cytotoxicity, enabling efficient delivery to combat drug-resistant infections.

Language: Английский

---

12-Hydroxy-Lauric Acid Tethered Self-Assembled Heterochiral Diphenylalanine-Based Mechanoresponsive and Proteolytically Stable Hydrogel: A Dual Player for Handling Cancer and Bacterial Challenges

ACS Applied Bio Materials, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 21, 2025

Deciphering the most promising strategy for evolution of cancer patient management remains a multifaceted, challenging affair to date. Additionally, such approaches often lead microbial infections as side effects, probably due compromised immunity patients undergoing treatment. Distinctly, this work delineates rational combinatorial harnessing stereogenic harmony in diphenylalanine fragment, tethering it an amphiphile 12-hydroxy-lauric acid at N-terminus (compounds I-III) that potential therapeutic could be extracted out from series. Aligned goal, cytotoxic properties and cell viability compounds were investigated using two distinct lines: MCF-7 (human breast cell) HEK 293 embryonic kidney). Our rigorous investigations revealed I-III exhibited substantial impact on MCF7 line. But pool three constructs, compound III (12-hydroxy-lauric -d-Phe-l-Phe-OH) showed better selectivity toward cancerous over normal comparison others, backed by computational calculations. Henceforth, was fished series used its elaborate anticancer activities reactive oxygen species generation, DNA fragmentation, caspase-dependent gene expression employing extrinsic intrinsic apoptotic factors well inflammatory biomarkers, namely, TNF-α IL1-β. We anticipated III, possessing mechanoresponsiveness nanofibrillar network, administered with injections because shear-thinning properties. Moreover, optimum partition coefficient might have allowed scaffold penetrate cellular membranes form dilactate complex (compound VI) when exposed accumulated lactates/lactic acids, common phenomenon observed within hypoxic tumor cores, accordance Warburg mechanism, thereby evading cytotoxicity cells. Besides, supramolecular β-sheets manifest antimicrobial efficacy against pathogens, Gram-positive bacteria, S. aureus B. subtilis, Gram-negative E. coli P. aeruginosa, fungus, C. albicans, along proteolytic stability high mechanical strength physiological pH. Overall, we speculate discovery these multifunctional bioinspired materials holds future promise preferential therapeutics remediation immune-susceptible patients, afflicted arising alone or effects chemotherapeutic medications.

Language: Английский

---

Tanfloc-Modified Titanium Surfaces: Optimizing Blood Coagulant Activity and Stem Cell Compatibility

ACS Biomaterials Science & Engineering, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 27, 2025

This study explores the synergistic effects of combining titania nanotubes (TiNTs) with biopolymer Tanfloc (TAN) to enhance surface properties TiNTs for biomedical applications. We investigated interactions blood components and human adipose-derived stem cells (ADSCs) TiNT surfaces covalently functionalized (TAN), an aminolyzed polyphenolic tannin derivative. The (TiNT-TAN) have great potential control protein adsorption platelet adhesion activation. Fluorescence scanning electron microscopy (SEM) were used analyze adherence amphoteric nature multiple functional groups on TAN can adsorption, adhesion, Further, modified supports cell (ADSC) viability, attachment, growth without any cytotoxic effect. conjugation significantly (****p < 0.0001) increased proliferation rate ADSCs compared surfaces.

Language: Английский

---

A Feasible Single-Solvent Nucleation and Growth Protocol for the Well-Defined Organization of Simple Porphyrins on Different Glass Composites

Journal of Composites Science, Journal Year: 2025, Volume and Issue: 9(3), P. 116 - 116

Published: March 1, 2025

Herein we report the nucleation and growth of porphyrin molecular chromophores using a single-solvent deposition protocol. Various glass substrates were investigated, aiming to investigate their impact on organization tetraphenyl-porphyrin (TPP) towards well-defined architectures. A variety aggregation morphologies obtained upon optimizing several parameters, including solvent temperature evaporation. This work demonstrates for first time that evaporation results in nanostructures, avoiding necessity mixed-solvent reprecipitation. Additionally, showed simple symmetrical porphyrins do not need presence self-assembling peptides, ions or amphiphiles induce capability forming structures. The presented herein open new avenues development complex highly ordered architectures from building blocks advanced materials with tailored properties.

Language: Английский

---

Evaluation of cylindrical micelles assembled from amphiphilic β-peptides as antigen delivery nanostructures

Nanoscale Advances, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Cylindrical micelles assembled from synthetic amphiphilic β-peptides elicit a robust antigen-specific immune response against the grafted epitope.

Language: Английский

---

How short peptides interact with oral cells? A systematic review

BMC Oral Health, Journal Year: 2025, Volume and Issue: 25(1)

Published: April 7, 2025

The study of short peptides in dentistry is a particularly promising area, due to their unique biological properties, such as antibacterial and antitumor activity, the ability modulate immune responses, tissue regeneration processes, potential stimulate stem cell differentiation. This provides systematic review aimed at analyzing role pathogenesis treatment oral diseases. A search was performed according PRISMA statement employed PICO(S) approach. limited English-language articles, vitro were included electronic search. comprehensive conducted across PubMed, Embase, Web Science Cochrane Library databases, without timeframe limits, resulting total 1085 scientific articles. After duplicate removal, 563 papers remained for further analysis. Following this screening process, 19 studies identified relevant topic. final selection 9 made based on type specific peptide examined. One last article eliminated analysis because laboratory methodology now refuted by more recent evidence. 8 dealing with different functions against cavity cells tissues included. 4 articles highlighted positively influence proliferation cavity. 2 antitumour activity peptides. 1 antimicrobial DJK-15. paper showed that Pep-B has anti-inflammatory properties towards However, main have been tested act are focus review. It can therefore be used basis possible hypothesis new compare molecules, vivo investigation action mechanisms applications drugs combat diseases Not applicable.

Language: Английский

---

Magnetically Driven Hydrogel Microrobots for Targeted Antibiotic Delivery

Chemistry - An Asian Journal, Journal Year: 2025, Volume and Issue: unknown

Published: April 22, 2025

Abstract Precise targeted delivery of antibiotics to infected sites is crucial for enhancing therapeutic efficacy and minimizing side effects. In this work, we present magnetic hydrogel‐based microrobots active antibiotic achieve bacterial elimination. These comprise a poly(acrylic acid‐co‐acrylamide) hydrogel microspheres encapsulating Fe 3 O 4 nanoparticle chains. The scaffold offers abundant spaces binding high‐capacity loading vancomycin via electrostatic interactions. embedded nanochains enable propulsion. Under rotating field ( H r t )), the can actively deliver their swarming motions, then eliminate bacteria (e.g., Staphylococcus aureus ) utilizing sustainable release on site. hold great promise as motile antibacterial platform treat infectious diseases.

Language: Английский

---

Triphenyl Phosphonium Functionalized Amphiphilic Peptides as Promising Antibacterial and Anticancer Agents

Chemical Communications, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Triphenyl phosphonium conjugated amphiphilic peptides are self-assembled into micelles that show potential antimicrobial activity against drug resistant strains.

Language: Английский

---

Self‐Assembled Short Peptide Amphiphile‐Gold Nanostructures: A Novel Approach for Bacterial Infection Treatment

Chemistry - An Asian Journal, Journal Year: 2025, Volume and Issue: unknown

Published: April 30, 2025

This study presents a simple and effective strategy for synthesizing biocompatible hybrid nanostructures composed of short peptide amphiphiles (sPA) gold nanoparticles (AuNPs) bacterial infection control. The self-assembling sPA molecules form stable β-sheet structures, which are further enhanced upon the addition ions (Au(III)) brief sunlight exposure, leading to formation functional AuNP-sPA nanostructures. Comprehensive spectroscopic microscopic characterization confirms successful integration AuNPs with sPA, resulting in nanomaterials potent antibacterial properties. conjugates exhibit superior activity against Gram-negative bacteria, including Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa high selectivity biocompatibility minimal cytotoxicity, reinforcing their therapeutic potential. These findings highlight as promising alternatives conventional antibiotics targeted treatment.

Language: Английский

---

A biomimetic nano-NET strategy for the treatment of MRSA-related implant-associated infection

RSC Advances, Journal Year: 2025, Volume and Issue: 15(19), P. 14821 - 14837

Published: Jan. 1, 2025

Schematic of a biomimetic nano-NET strategy based on PCPNAs for the treatment MRSA-related implant-associated infections.

Language: Английский

---