Lighting the Path: Raman Spectroscopy’s Journey Through the Microbial Maze DOI Creative Commons
Markus Salbreiter, Sandra Baaba Frempong,

Shimon Even

et al.

Molecules, Journal Year: 2024, Volume and Issue: 29(24), P. 5956 - 5956

Published: Dec. 17, 2024

The rapid and precise identification of microorganisms is essential in environmental science, pharmaceuticals, food safety, medical diagnostics. Raman spectroscopy, valued for its ability to provide detailed chemical structural information, has gained significant traction these fields, especially with the adoption various excitation wavelengths tailored optical setups. choice wavelength setup spectroscopy influenced by factors such as applicability, cost, whether bulk or single-cell analysis performed, each impacting sensitivity specificity bacterial detection. In this study, we investigate potential different identification, utilizing a mock culture composed six species: three Gram-positive (S. warneri, S. cohnii, E. malodoratus) Gram-negative (P. stutzeri, K. terrigena, coli). To improve classification, applied machine learning models analyze extract unique spectral features from data. results indicate that significantly influences spectra obtained, thereby accuracy effectiveness subsequent classification results.

Language: Английский

Phage‐derived proteins: Advancing food safety through biocontrol and detection of foodborne pathogens DOI Creative Commons
Dahee Choi,

Sangryeol Ryu,

Minsuk Kong

et al.

Comprehensive Reviews in Food Science and Food Safety, Journal Year: 2025, Volume and Issue: 24(2)

Published: Feb. 3, 2025

Abstract The emergence of antimicrobial‐resistant foodborne pathogens poses a continuous health risk and economic burden as they can easily spread through contaminated food. Therefore, the demand for new antimicrobial agents to address this problem is steadily increasing. Similarly, development rapid, sensitive, accurate pathogen detection tools prerequisite ensuring food safety. Phage‐derived proteins have become innovative combating these because their potent activity host specificity. Phage are relatively free from regulation compared phages per se, there no concerns about transduction harmful genes. With recent progress in next‐generation sequencing technology, analysis phage genomes has more accessible, numerous with potential biocontrol been identified. This review provides comprehensive overview protein research on safety 2006 present, pivotal period marked by certification Generally Recognized As Safe (GRAS). Emphasizing advancements, we investigated diverse applications various purposes. While highlighting successful implementation proteins, also current bottlenecks propose strategies overcome challenges. By summarizing state phage‐derived contributes deeper understanding effective detecting pathogens.

Language: Английский

Citations

1
A practical approach to quantitative analytical surface-enhanced Raman spectroscopy DOI Creative Commons

Yikai Xu,

Wafaa Aljuhani, Yingrui Zhang

et al.

Chemical Society Reviews, Journal Year: 2024, Volume and Issue: unknown

Published: Jan. 1, 2024

The high sensitivity, molecular specificity and speed of analysis make SERS an attractive analytical technique. This review draws out the underlying principles for provides practical tips tricks quantitation.

Language: Английский

Citations

7
Illuminating extracellular nanovesicles through the spectroscopic lens: a mini review of cutting-edge insights and emerging applications DOI Creative Commons

Debarati Bhowmik,

Markand Patel, Pola Goldberg Oppenheimer

et al.

Frontiers in Bioengineering and Biotechnology, Journal Year: 2025, Volume and Issue: 13

Published: May 9, 2025

Extracellular vesicles (EVs) are cell-derived particles that facilitate intercellular communication by carrying bioactive molecules like proteins and RNA, impacting both health disease. Herein, the EVs' significance in physiological pathological processes is reviewed, emphasising their potential as biomarkers for diseases including instance, cancer, neurodegenerative disorders cardiovascular conditions. The principles applications of Raman spectroscopy (RS) - a powerful tool offering detailed molecular insights into EVs, further examined. non-destructive nature this spectroscopic technique renders it invaluable studying composition, purity concentration EVs. When EVs isolated from accessible biofluids such blood, urine or saliva, overall process remains minimally invasive, enhancing its clinical applicability. review highlights spectroscopy’s role identifying disease-related distinguishing subpopulations our understanding disease mechanisms therapeutic applications.

Language: Английский

Citations

0
Lighting the Path: Raman Spectroscopy’s Journey Through the Microbial Maze DOI Creative Commons
Markus Salbreiter, Sandra Baaba Frempong,

Shimon Even

et al.

Molecules, Journal Year: 2024, Volume and Issue: 29(24), P. 5956 - 5956

Published: Dec. 17, 2024

The rapid and precise identification of microorganisms is essential in environmental science, pharmaceuticals, food safety, medical diagnostics. Raman spectroscopy, valued for its ability to provide detailed chemical structural information, has gained significant traction these fields, especially with the adoption various excitation wavelengths tailored optical setups. choice wavelength setup spectroscopy influenced by factors such as applicability, cost, whether bulk or single-cell analysis performed, each impacting sensitivity specificity bacterial detection. In this study, we investigate potential different identification, utilizing a mock culture composed six species: three Gram-positive (S. warneri, S. cohnii, E. malodoratus) Gram-negative (P. stutzeri, K. terrigena, coli). To improve classification, applied machine learning models analyze extract unique spectral features from data. results indicate that significantly influences spectra obtained, thereby accuracy effectiveness subsequent classification results.

Language: Английский

Citations

0