Effect of Caloric Restriction Combined with Probiotic Supplementation on Parameters Related to Weight Loss, Homeostatic, and Hedonic Systems of Food Intake in Adult Men Living with Obesity: Secondary Data from a Randomized, Parallel, Double-Blind, Placebo-Controlled Clinical Trial DOI Creative Commons
Marcus Vinicius Lúcio dos Santos Quaresma,

Glaice Aparecida Lucin,

Camila Moraes Marques

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 9, 2024

Abstract We aimed to investigate the effect of CR with probiotic supplementation on weight loss and parameters related homeostatic hedonic systems. It is a randomized, double-blind, parallel, placebo-controlled clinical trial (UTN: U1111-1247-9906). include obese adult males, aged 25 44 years. The established was 30% daily energy expenditure. Probiotic was: two sachets 1 g each 1×10⁹ CFU Lactobacillus acidophilus NCFM, rhamnosus HN001, paracasei Lpc-37, Bifidobacterium lactis HN019. primary outcomes: body composition. Secondary leptin, ghrelin, GLP-1, glucose-dependent insulinotropic polypeptide (GIP), insulin, peptide YY (PYY), adiponectin. Additionally, binge eating, emotional cognitive restraint were assessed by Three-Factor Eating Questionnaire. study includes per protocol analyses (CR + placebo [CRPLA] n = 12 vs. [CRPRO] 13). Body mass (F 65.9; p < 0.001) decreased after 4 weeks (p 0.001). A similar result found for absolute fat 71.0; Leptin levels 13.2; 0.002) 0.001), without group or interaction effect. Moreover, GLP-1 did not change intervention 2.29; 0.114). Likewise, GIP 2.0; 0.148) adiponectin 3.14; 0.053) change. Binge eating reduced 16.5; only in CRPRO 0.018). Emotional 9.44; 0.005), but 0.047). Finally, increased 43.2; CRPLA 0.003) groups. In summary, fat, as well leptin restraint. reductions scores seen group.

Language: Английский

Genome sequence and evaluation of safety and probiotic potential of Lacticaseibacillus paracasei LC86 and Lacticaseibacillus casei LC89 DOI Creative Commons
Ting Chen,

Yunjiao Zhao,

Yixuan Fan

et al.

Frontiers in Microbiology, Journal Year: 2025, Volume and Issue: 15

Published: Jan. 27, 2025

Aim A comprehensive safety assessment of potential probiotic strains was essential for their application in the food industry. This article systematically evaluated characteristics, whole-genome sequence analysis and Lacticaseibacillus paracasei LC86 casei LC89. Methods Firstly, two lactic acid bacteria selected were identified. Secondly, sequencing performed on LC89, antibiotic resistance, pathogenicity, virulence genes analyzed. We tested various properties strains, included tolerance, cell adhesion, hemolytic activity, catalase gelatin hydrolysis, arginine hydrolysis ability, bile salt capacity, mucin degradation, bioamine, D-/L-lactic production susceptibility, to confirm LC89 both vitro vivo . Additionally, we studied acute toxicity mice through a 14-day oral gavage experiment. Results The identified as genomes devoid virulence, resistance pathogenicity genes. exhibited good tolerance temperature, artificial gastric fluid intestinal fluid; they non-hemolytic, all negative. They capability break down proteins demonstrated sensitivity range antibiotics. LD 50 &gt;2 × 10 CFU/kg. Conclusion experimental results above characteristics indicating candidates probiotics human animal applications.

Language: Английский

Citations

2
Granola consumption with multiple prebiotics in Japanese participants increases Bifidobacterium abundance and improves stress and subjective sleepiness DOI Creative Commons
Hiroyuki Sasaki,

Hirofumi Masutomi,

Shuji Nakamura

et al.

Frontiers in Nutrition, Journal Year: 2025, Volume and Issue: 12

Published: March 20, 2025

Background Sleep is essential for physical and mental health. However, stress-related sleep disorders are common in Japan, the gut–brain axis may play a role stress management. This study investigated whether consumption of granola containing multiple prebiotic ingredients could alleviate improve insomnia adults with problems, regardless individual differences gut microbiota. Additionally, we aimed to investigate relationship between changes microbiota observed improvements. Method A single-arm uncontrolled trial was conducted 27 high levels disturbance. The participants consumed 50 g prebiotics-containing daily 8 weeks. Subjective quality assessed using Athens Insomnia Scale, Epworth Oguri-Shirakawa-Azumi Inventory-Middle-aged Aged version (OSA-MA). Stress were by administering Brief Job Questionnaire Profile Mood States 2nd edition (POMS2). Gut composition analyzed 16S rDNA sequencing. Results After weeks, subjective scores onset maintenance improved significantly, whereas mood disturbance decreased significantly. analysis showed that relative abundance Bifidobacterium increased, Bacteroides decreased. Correlation suggested significant association increased level reduced ( r = −0.39, p 0.0035) −0.3, 0.026). Conclusion Prebiotics-containing disturbances, which be attributed alterations microbiota, particularly increase abundance.

Language: Английский

Citations

0
Effect of Probiotics Supplementation on Cortisol Levels: A Systematic Review and Meta-Analysis DOI Open Access
Manav Jain, Aishwarya Anand, Nisha Sharma

et al.

Nutrients, Journal Year: 2024, Volume and Issue: 16(20), P. 3564 - 3564

Published: Oct. 21, 2024

Background: Several randomized controlled trials (RCTs) have shown conflicting results on cortisol levels following probiotic administration in healthy and diseased populations. Previous analyses were inconclusive due to limited studies, evidence is lacking how these effects vary by health status; region; therapy duration; medications, use of single or multiple strains. Methods: In this systematic review meta-analysis (PROSPERO [CRD42024538539]), we searched PubMed, Cochrane Library, Embase, Scopus, Web Science, CINAHL, ProQuest, Science Preprints until 13 August 2024, for RCTs administration, either alone combined, across all age groups without specific medical condition requirements. We applied random-effects meta-analysis, assessed bias using the RoB 2 tool, evaluated certainty with GRADE. Findings: screened 1739 records retrieved 46 (3516 participants). Probiotics supplementation decreased compared control arm [46 RCTs; SMD: −0.45; 95% CI: −0.83; −0.07; I2: 92.5%, low certainty]. Among various subgroups; probiotics subgroups concomitant medications [37 −0.30; CI [−0.58; −0.03], 88.7%] a strain [30 −0.33; −0.63; −0.028; 88.8%], population [35 SMD:−0.3; −0.58; −0.03; 88.7] Asia region [21 −1.58; 95%]. Interpretation: A level suggests might reduce levels, but more targeted studies are needed identify variables affecting response subgroups.

Language: Английский

Citations

2
Effect of Caloric Restriction Combined with Probiotic Supplementation on Parameters Related to Weight Loss, Homeostatic, and Hedonic Systems of Food Intake in Adult Men Living with Obesity: Secondary Data from a Randomized, Parallel, Double-Blind, Placebo-Controlled Clinical Trial DOI Creative Commons
Marcus Vinicius Lúcio dos Santos Quaresma,

Glaice Aparecida Lucin,

Camila Moraes Marques

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 9, 2024

Abstract We aimed to investigate the effect of CR with probiotic supplementation on weight loss and parameters related homeostatic hedonic systems. It is a randomized, double-blind, parallel, placebo-controlled clinical trial (UTN: U1111-1247-9906). include obese adult males, aged 25 44 years. The established was 30% daily energy expenditure. Probiotic was: two sachets 1 g each 1×10⁹ CFU Lactobacillus acidophilus NCFM, rhamnosus HN001, paracasei Lpc-37, Bifidobacterium lactis HN019. primary outcomes: body composition. Secondary leptin, ghrelin, GLP-1, glucose-dependent insulinotropic polypeptide (GIP), insulin, peptide YY (PYY), adiponectin. Additionally, binge eating, emotional cognitive restraint were assessed by Three-Factor Eating Questionnaire. study includes per protocol analyses (CR + placebo [CRPLA] n = 12 vs. [CRPRO] 13). Body mass (F 65.9; p < 0.001) decreased after 4 weeks (p 0.001). A similar result found for absolute fat 71.0; Leptin levels 13.2; 0.002) 0.001), without group or interaction effect. Moreover, GLP-1 did not change intervention 2.29; 0.114). Likewise, GIP 2.0; 0.148) adiponectin 3.14; 0.053) change. Binge eating reduced 16.5; only in CRPRO 0.018). Emotional 9.44; 0.005), but 0.047). Finally, increased 43.2; CRPLA 0.003) groups. In summary, fat, as well leptin restraint. reductions scores seen group.

Language: Английский

Citations

0