Bipolar electrochemically generated fluorescence detector for microchip electrophoresis with and without a potentiostat: Application to reducible analyte detection DOI Creative Commons
Manjula B. Wijesinghe,

Indika Kasun Warnakula,

Dulan B. Gunasekara

et al.

Sensors and Actuators Reports, Journal Year: 2025, Volume and Issue: 9, P. 100283 - 100283

Published: Jan. 14, 2025

Microchip electrophoresis (ME) is a separation method that makes it possible to quickly analyze multiple species in small volume samples miniaturized format. Two commonly employed detection approaches for ME are electrochemistry and fluorescence. Electrochemical can be direct of analytes via redox chemistry, while fluorescence generally offers lower limits but often requires analyte derivatization. The present study uses bipolar couple the two methods into single device as detector encompasses advantages both techniques. This system employs closed electrode (BPE) converts cathodic amperometric current produced at (sensing) pole response generated reporting pole. model analytes, benzoquinone resazurin, were separated detected using with reductive sensing BPE. A potentiostat was used control electrode. then converted through oxidation 2,7-dichlorodihydrofluorescein channel. subsequently modified run without by voltage bias BPE applied across channel generate electroosmotic flow well corresponding potentiostat-free mode evaluated determination 3-nitro-l-tyrosine, reducible biomarker oxidative stress. Lastly, sacrificial electrochemically active species, Trolox®, incorporated compensate background detection.

Language: Английский

Real-Time Visualization of Endogenous H2O2 Production in Mammalian Spheroids by Electrochemiluminescence DOI Creative Commons

Vanshika Gupta,

Francesco Falciani, Brady R. Layman

et al.

Chemical & Biomedical Imaging, Journal Year: 2025, Volume and Issue: 3(5), P. 310 - 321

Published: March 10, 2025

Two-dimensional cell culture may be insufficient when it comes to understanding human disease. The redox behavior of complex, three-dimensional tissue is critical disease genesis and propagation. Unfortunately, few measurement tools are available for such models yield quantitative insight into how reactive oxygen species (ROS) form over time. Here, we demonstrate an imaging platform the real-time visualization H2O2 formation mammalian spheroids made noncancerous embryonic kidney cells (HEK-293) metastatic breast cancer (MCF-7 MDA-MB-231). We take advantage luminol electrochemiluminescence reaction on a transparent tin-doped indium oxide electrode. luminescence this as function [H2O2] linear (R2 = 0.98) with dynamic range between 0.5 μM 0.1 mM, limit detection 2.26 ± 0.58 μM. Our method allows observation ROS activity in growing days advance current techniques without need sacrifice sample postanalysis. Finally, use our procedure key pathways cancerous can up-regulated downregulated through addition common metabolic drugs, rotenone carbonyl cyanide-p-trifluoromethoxyphenylhydrazone. results suggest that Warburg Effect studied single spheroids, drugs one implicate specific formation. expect diagnostic tool have wide applications propagation system more closely related tissue.

Language: Английский

Citations

2
Photo-Crosslinking of Doped Magic-size Nanocluster for the Construction of Enhanced Electrochemiluminescence Biosensors DOI Creative Commons

Junjun Ge,

Tengyue Yin,

Haoyang Zhang

et al.

Chemical Science, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

We developed a bipolar electrode-based ECL sensing platform using doping and direct optical crosslinking of semiconductor magic-sized nanoclusters, achieving significant improvements in both intensity stability.

Language: Английский

Citations

1
Exploring the Diffusion of DNA Strands into Nanoporous Structure for Establishing a Universal Electrochemical Biosensor DOI Creative Commons

Cong-Lin Zhao,

Runlei Gao,

Yinzheng Niu

et al.

Chemical Science, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 30, 2024

The development of universal electrochemical sensing platforms with high sensitivity and specificity is great significance for advancing practical disease diagnostic methods devices. Exploring the structural properties electrode materials their interaction biomolecules essential to developing novel distinctive analytical approaches. Here, we innovatively investigated effect DNA length configuration on molecule transfer into nanostructure a nanoporous gold (NPG) electrode. NPG can not only distinguish quantify short strands but also prevent diffusion long DNA, thereby minimizing or eliminating background interference. Leveraging these findings, developed DNA-based biosensing platform detection different types biomolecules. As proof-of-concept, this was integrated nuclease-assisted target-recycling recognition amplification reactions achieve sensitive specific single-stranded microRNA-21, carcino-embryonic antigen, limits 4.09, 27.4, 0.28 fM, respectively. demonstrated universality, sensitivity, specificity, capability analyzing complex samples ensure comprehensive robust approach nucleic acid-based molecular diagnosis.

Language: Английский

Citations

4
Study on the Electrochemiluminescence Emission Mechanism of HOF-14 and Its Multimode Sensing and Imaging Application DOI
Qianqian Cai, Hongkun Li, Zhikang Li

et al.

Analytical Chemistry, Journal Year: 2024, Volume and Issue: 96(42), P. 16900 - 16909

Published: Oct. 9, 2024

A novel hydrogen-bonded organic framework (HOF-14) has attracted much attention due to its excellent biocompatibility and low toxicity, but research in the electrochemiluminescence (ECL) field not been reported. In this work, annihilation-type coreactant-type ECL emission mechanisms of HOF-14 were studied systematically for first time. It was found that quantum efficiency HOF-14/TEA coreactant system highest, which 1.82 times Ru(bpy)32+/TEA. Further, intensity could achieve colorimetric (CL) imaging mobile phone. We also discovered had superior photoelectrochemical (PEC) performance. Based on above results, a unique HOF-14-based multimode sensing platform constructed. The antibiotic Enrofloxacin (ENR) selected as detection target, Cu–Zn bimetallic single-atom nanozyme (Cu–Zn/SAzyme) with peroxidase (POD)-like activity used prepare quenching probes. When target ENR present, Cu–Zn/SAzyme probes introduced surface by dual-aptamer sandwich method. catalyze diaminobenzidine (DAB) produce brown precipitations quench ECL, PEC, CL signals HOF-14, realizing ENR. This work only PEC property new material mechanism proposed platform, greatly improved accuracy showed great contributions analysis.

Language: Английский

Citations

3
Bipolar electrochemically generated fluorescence detector for microchip electrophoresis with and without a potentiostat: Application to reducible analyte detection DOI Creative Commons
Manjula B. Wijesinghe,

Indika Kasun Warnakula,

Dulan B. Gunasekara

et al.

Sensors and Actuators Reports, Journal Year: 2025, Volume and Issue: 9, P. 100283 - 100283

Published: Jan. 14, 2025

Microchip electrophoresis (ME) is a separation method that makes it possible to quickly analyze multiple species in small volume samples miniaturized format. Two commonly employed detection approaches for ME are electrochemistry and fluorescence. Electrochemical can be direct of analytes via redox chemistry, while fluorescence generally offers lower limits but often requires analyte derivatization. The present study uses bipolar couple the two methods into single device as detector encompasses advantages both techniques. This system employs closed electrode (BPE) converts cathodic amperometric current produced at (sensing) pole response generated reporting pole. model analytes, benzoquinone resazurin, were separated detected using with reductive sensing BPE. A potentiostat was used control electrode. then converted through oxidation 2,7-dichlorodihydrofluorescein channel. subsequently modified run without by voltage bias BPE applied across channel generate electroosmotic flow well corresponding potentiostat-free mode evaluated determination 3-nitro-l-tyrosine, reducible biomarker oxidative stress. Lastly, sacrificial electrochemically active species, Trolox®, incorporated compensate background detection.

Language: Английский

Citations

0