Overview of the Design and Application of Dual-Signal Immunoassays

Molecules, Journal Year: 2024, Volume and Issue: 29(19), P. 4551 - 4551

Published: Sept. 25, 2024

Immunoassays have been widely used for the determination of various analytes in fields disease diagnosis, food safety, and environmental monitoring. Dual-signal immunoassays are now advanced integrated detection technologies with excellent self-correction self-validation capabilities. In this work, we summarize recent advances development optical electrochemical dual-signal immunoassays, including colorimetric, fluorescence, surface-enhanced Raman spectroscopy (SERS), electrochemical, electrochemiluminescence, photoelectrochemical methods. This review particularly emphasizes working principle diverse utilization dual-functional molecules nanomaterials. It also outlines challenges prospects future research on immunoassays.

Language: Английский

---

Enhancing lateral flow immunoassay performance for cardiac troponin I detection with pore-size tailored silica nanoparticles and smartphone-based “AdaptiScan” analysis

Frontiers in Bioengineering and Biotechnology, Journal Year: 2025, Volume and Issue: 13

Published: March 26, 2025

The accurate and rapid detection of cardiac troponin I (cTnI) at the point care is crucial for timely diagnosis myocardial infarction (MI). This study introduces an advanced lateral flow immunoassay (LFIA) platform cTnI detection. We employed small-sized, large-pore dendritic mesoporous silica nanoparticles (DMSN-2) to encapsulate quantum dots (QDs), achieving enhanced QD loading capacity 1.427 g QD/g silica, compared 0.881 smaller pore counterparts (DMSN-1). nano-LFIA was further integrated with “AdaptiScan”, a smartphone-based system that uses adaptive algorithms automatically extract analyze fluorescence signals from LFIA strips. integration pore-size tailored DMSNs “AdaptiScan” resulted in limit 42.6 ng/L, which meets clinical diagnostic requirements. offers sensitive, cost-effective, portable solution MI, potentially transforming point-of-care testing resource-limited settings.

Language: Английский

---

Enhancing the Peroxidase-Mimicking Activity of Gold Nanoparticles for Lateral Flow Assays: Quantitative Evaluation in a Kinetic View

Langmuir, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 14, 2025

Highly sensitive lateral flow immunoassays (LFIAs) are essential for various point-of-care applications, and gold nanoparticles (Au NPs) by far the most commonly used labels. However, conventional LFIAs often suffer from high detection limits (LOD) or low sensitivity. In this study, we investigated three strategies to enhance sensitivity of improving peroxidase-mimicking (POD) activity Au NPs. The POD unmodified NPs was negligible (<0.01 units/mg, U/mg). first strategy involved coupling with horseradish peroxidase (HRP), which increased 65 U/mg. second approach forming a thin palladium iridium shell on NPs, elevated 0.69-0.71 third binding mercury ions (Hg2+) resulting in up 3 Finally, developed simple quantitative model estimate LOD based kinetic parameters. Using Au-HRP conjugates, demonstrated that experimentally measured consistent calculated values. provides framework evaluating catalytic signal amplification can be guide development highly assays.

Language: Английский

---

In vitro diagnosis based on SERS–LFIA: research hotspots, increase sensitivities, combined detection, multimodal detection and related patents

Journal of Materials Chemistry B, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

The schematic overview of in vitro diagnosis based on SERS-LFIA.

Language: Английский

---

Advances in enhancement-type signal tracers and analysis strategies driven Lateral flow immunoassay for guaranteeing the agri-food safety

Biosensors and Bioelectronics, Journal Year: 2024, Volume and Issue: 268, P. 116920 - 116920

Published: Nov. 7, 2024

Language: Английский

---