Frenemies on the reef? Resolving the coral–Endozoicomonas association

Trends in Microbiology, Journal Year: 2024, Volume and Issue: 32(5), P. 422 - 434

Published: Jan. 11, 2024

Language: Английский

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Deciphering the probiotic properties and safety assessment of a novel multi-stress-tolerant aromatic yeast Pichia kudriavzevii HJ2 from marine mangroves

Food Bioscience, Journal Year: 2023, Volume and Issue: 56, P. 103248 - 103248

Published: Oct. 20, 2023

Language: Английский

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Evaluation of the probiotic potential of yeast isolated from kombucha in New Zealand

Current Research in Food Science, Journal Year: 2024, Volume and Issue: 8, P. 100711 - 100711

Published: Jan. 1, 2024

The current study investigated the in vitro probiotic potential of yeast isolated from kombucha, a tea beverage fermented with symbiotic culture acetic acid bacteria and yeast. A total 62 strains were previously four different commercial kombucha samples sold New Zealand. Fifteen representative isolates belonging to eight species evaluated for their growth under conditions (temperature, low pH, concentrations bile salts, NaCl). Cell surface characteristics, functional enzymatic activities selected also studied triplicate experiments. Results showed that six (Dekkera bruxellensis LBY1, Sachizosaccharomyces pombe LBY5, Hanseniaspora valbyensis DOY1, Brettanomyces anomalus DOY8, Pichia kudraivzevii GBY1, Saccharomyces cerevisiae GBY2) able grow low-acid (at pH 2 3) presence salts. This suggests survive passage through human gut. All 15 exhibited negative activity reactions (haemolytic, gelatinase, phospholipase, protease activities), thus, they can be considered safe consume. Notably, two fifteen (Pichia GBY1 desirable cell hydrophobicity (64.60–83.87%), auto-aggregation (>98%), co-aggregation, resistance tested antibiotics (ampicillin, chloramphenicol, colistin sulphate, kanamycin, nalidixic acid, nitrofurantoin, streptomycin, tetracycline), high levels antioxidant (>90%). Together, our data reveal GBY2 application food production.

Language: Английский

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Protective Effect of Probiotics against Pseudomonas aeruginosa Infection of Human Corneal Epithelial Cells

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(3), P. 1770 - 1770

Published: Feb. 1, 2024

Probiotic therapy needs consideration as an alternative strategy to prevent and possibly treat corneal infection. This study aimed assess the preventive effect of Lactobacillus reuteri Bifidobacterium longum subsp. infantis on reducing infection human epithelial (HCE) cells caused by Pseudomonas aeruginosa. The probiotics’ against was evaluated in cell monolayers pretreated with each probiotic 1 h 24 prior P. aeruginosa challenge followed growth combination. Cell adhesion, cytotoxicity, anti-inflammatory, antinitrosative activities were evaluated. L. B. adhered HCE cells, preserved occludin tight junctions’ integrity, increased mucin production a SkinEthicTM model. Pretreatment or significantly protected from at h, increasing viability 110% (110.51 ± 5.15; p ≤ 0.05) 137% (137.55 11.97; 0.05), respectively. Each showed anti-inflammatory activities, TNF-α level (p 0.001) NOx amount reestablishing IL-10 0.001). In conclusion, this demonstrated that exert protective effects context restoring modulating inflammatory cytokine release.

Language: Английский

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Morphogenesis of bacterial cables in polymeric environments

Science Advances, Journal Year: 2025, Volume and Issue: 11(3)

Published: Jan. 17, 2025

Many bacteria live in polymeric fluids, such as mucus, environmental polysaccharides, and extracellular polymers biofilms. However, laboratory studies typically focus on cells polymer-free fluids. Here, we show that interactions with shape a fundamental feature of bacterial life—how they proliferate space multicellular colonies. Using experiments, find when polymer is sufficiently concentrated, generically reversibly form large serpentine “cables” proliferate. By combining experiments biophysical theory simulations, demonstrate this distinctive colony morphogenesis arises from an interplay between polymer-induced entropic attraction neighboring their hindered ability to diffusely separate each other viscous solution. Our work thus reveals pivotal role sculpting proliferating colonies, implications for how interact hosts the natural environment, uncovers quantitative principles governing complex environments.

Language: Английский

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Physiological characteristics of Bacillus strains originated from dairy products and their impacts on rheological properties of pasteurised yoghurt

International Journal of Dairy Technology, Journal Year: 2025, Volume and Issue: 78(1)

Published: Feb. 1, 2025

Due to the strong stress resistance, causing food spoilage and toxin production of spore‐forming Bacillus species, their presence will be detrimental quality assurance dairy products. Impacts five strains ( licheniformis H1, B. H2, subtilis Z2, cereus Z6‐1 Z6‐2) originated from products on pasteurised yoghurt were investigated. Physiological characteristics strains, including growth, acid production, proteolysis, amylase extracellular polysaccharide biofilm formation, analysed. Additionally, changes in ‐contaminated during a 60‐day incubation at 28°C observed. strongly strain‐specific. Both had highest growth rate Brian Heart Infusion broth strongest proteolytic ability skimmed milk. Z2 exhibited activity, while yields polysaccharides obtained Z6‐2 H2. The acidic environment (about pH 4.2–4.4) was not suitable for Bacilli , as low survival after inoculation slow growth. Yoghurt contaminated with H1 showed resulting gel relatively elasticity. containing H2 high leading formation many interspersed holes. strain effect rheological properties yoghurt, lowest viscoelasticity. Protease mainly interferes thickness protein matrix affects size holes gel. residual may adversely affect particularly long shelf life.

Language: Английский

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Type 1 fimbriae-mediated collective protection against type 6 secretion system attacks

mBio, Journal Year: 2024, Volume and Issue: 15(4)

Published: March 18, 2024

Bacterial competition may rely on secretion systems such as the type 6 system (T6SS), which punctures and releases toxic molecules into neighboring cells. To subsist, bacterial targets must counteract threats posed by T6SS-positive competitors. In this study, we used a comprehensive genome-wide high-throughput screening approach to investigate dynamics of interbacterial competition. Our primary goal was identify deletion mutants within well-characterized

Language: Английский

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Pseudomonas aeruginosa Biofilm Lifecycle: Involvement of Mechanical Constraints and Timeline of Matrix Production

Antibiotics, Journal Year: 2024, Volume and Issue: 13(8), P. 688 - 688

Published: July 24, 2024

Pseudomonas aeruginosa is an opportunistic pathogen causing acute and chronic infections, especially in immunocompromised patients. Its remarkable adaptability resistance to various antimicrobial treatments make it difficult eradicate. persistence enabled by its ability form a biofilm. Biofilm community of sessile micro-organisms self-produced extracellular matrix, which forms scaffold facilitating cohesion, cell attachment, micro- macro-colony formation. This lifestyle provides protection against environmental stresses, the immune system, treatments, confers capacity for colonization long-term persistence, often characterizing infections. In this review, we retrace events life cycle P. biofilm, from surface perception/contact spreading. We focus on importance appendages, mechanical constraints, kinetics matrix component production each step biofilm cycle.

Language: Английский

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Genomic Insight Into Lacticaseibacillus paracasei SP5, Reveals Genes and Gene Clusters of Probiotic Interest and Biotechnological Potential

Frontiers in Microbiology, Journal Year: 2022, Volume and Issue: 13

Published: June 16, 2022

The Lacticaseibacillus paracasei species is comprised by nomadic bacteria inhabiting a wide variety of ecological niches, from fermented foodstuffs to host-associated microenvironments. Lc. SP5 novel strain, originally isolated kefir grains that presents desirable probiotic and biotechnological attributes. In this study, we applied genomic tools further characterize the potential strain. Firstly, whole genome sequencing assembly, were performed construct chromosome map strain determine its stability. carriers several insertion sequences, however, no plasmids or mobile elements detected. Furthermore, phylogenomic comparative analyses utilized study attributes more specifically, metabolic capacity ability withstand environmental stresses imposed during food processing passage through gastrointestinal (GI) tract. More Kyoto Encyclopedia Genes Genomes (KEGG) Carbohydrate-active enzyme (CAZymes) provided evidence for stain utilize an array carbohydrates as growth substrates. Consequently, genes heat, cold, osmotic shock, acidic pH, bile salt tolerance annotated. Importantly bioinformatic analysis showed does not harbor acquired antimicrobial resistance nor virulence factors, in agreement with previous experimental data. Putative bacteriocin biosynthesis clusters identified using BAGEL4, suggesting activity. Concerning microbe-host interactions, adhesins, moonlighting proteins, exopolysaccharide (EPS) pilins mediating adhesive phenotype were, also, pinpointed SP5. Validation was employing microbiological method confocal microscopy. Conclusively, harbors necessary manifestation character application industry. Upcoming studies will focus on mechanisms action at multiple levels.

Language: Английский

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Lactobacillus cell envelope-coated nanoparticles for antibiotic delivery against cariogenic biofilm and dental caries

Journal of Nanobiotechnology, Journal Year: 2022, Volume and Issue: 20(1)

Published: Aug. 2, 2022

Abstract Background Due to their prevalence, dental caries ranks first among all diseases endangering human health. Therefore, the prevention of is great significance, as have become a serious public health problem worldwide. Currently, using nanoscale drug delivery systems prevent has received increased attention. However, preventive efficacy these substantially limited due unique physiological structure cariogenic biofilms. Thus, novel strategies aimed at combating biofilms improve efficiency against are meaningful and very necessary. Herein, inspired by cell membrane coating technology Lactobacillus strains, we coated triclosan (TCS)-loaded poly(lactic-co-glycolic acid) (PLGA) nanoparticles (TCS@PLGA-NPs) with an envelope (LA/TCS@PLGA-NPs) investigated potential nanoparticle system caries. Results LA/TCS@PLGA-NPs were successfully prepared favorable properties, including envelope, controllable size, negative charge, sustained drug-release kinetics so on. The inherited native properties from source surface, thus adhered S. mutans , integrated into biofilm, interfered biofilm formation . significantly inhibited activity, biomass virulence gene expression in vitro. Additionally, exhibited long-lasting inhibitory effect on progression vivo. safety performance also favorable. Conclusions Our findings reveal that antibiofilm relies not only inheritance surface but could be considered feasible candidates for new class effective Furthermore, this work provides insights presents strategy combat bacterial associated infections. Graphical

Language: Английский

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