Epithelial apoptosis: A back-and-forth mechanical interplay between the dying cell and its surroundings

Seminars in Cell and Developmental Biology, Journal Year: 2025, Volume and Issue: 168, P. 1 - 12

Published: Feb. 21, 2025

Language: Английский

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SLMAP3 is essential for neurulation through mechanisms involving cytoskeletal elements, ABP, and PCP

Life Science Alliance, Journal Year: 2024, Volume and Issue: 7(12), P. e202302545 - e202302545

Published: Oct. 4, 2024

SLMAP3 is a tail-anchored membrane protein that targets subcellular organelles and believed to regulate Hippo signaling. The global loss of causes late embryonic lethality in mice, with some embryos exhibiting neural tube defects such as craniorachischisis. We show here −/− display reduced length increased width plates, signifying arrested convergent extension. expression planar cell polarity (PCP) components Dvl2/3 the activity downstream ROCK2, cofilin, JNK1/2 were dysregulated E12.5 brains. Furthermore, cytoskeletal proteins (γ-tubulin, actin, nestin) apical (PKCζ ZO-1) mislocalized tubes embryos, subsequent decrease colocalization PCP (Fzd6 pDvl2). However, no changes or cytoskeleton found cultured neuroepithelial cells depleted SLMAP3, suggesting an essential requirement for these processes vivo neurulation. had impact on signaling brains, tubes. Proteomic analysis revealed interactome components, including nestin, tropomyosin 4, intermediate filaments, plectin, SCRIB, STRIPAK members These results reveal crucial role development by regulating organization pathway.

Language: Английский

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A multi-tiered mechanical mechanism shapes the early neural plate

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: June 21, 2023

Abstract The formation of complex tissues during embryonic development requires an intricate spatiotemporal coordination local mechanical processes regulating global tissue morphogenesis. Here, we uncover a novel mechanism that mechanically regulates the shape anterior neural plate (ANP), vital forebrain precursor, zebrafish gastrulation. Combining in vivo and silico approaches reveal ANP is shaped by flows regulated distinct force generating processes. We show mesendoderm migration E-cadherin-dependent differential interactions control flow regimes neuroectoderm. Initial opposing lead to progressive folding neuroectoderm internalisation which turn provide forces driving reshaping. find convergent extension dispensable for but required extension. Our results highlight how regulation coupling different between embryo controls first event developing brain.

Language: Английский

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Molecular and morphogenetic features of neurulation

Zaporozhye Medical Journal, Journal Year: 2024, Volume and Issue: 26(1), P. 72 - 77

Published: Feb. 5, 2024

Neurulation occurs by two different mechanisms, called primary and secondary neurulation. In humans, neurulation along most of the rostrocaudal axis embryo, while caudally, only in lower sacral coccygeal regions. Primary is responsible for a change neural plate shape, lateral edges which rise then converge at dorsal midline to merge into tube. Initially, tube, formed as result neurulation, open both ends through so-called rostral caudal neuropores. These neuropores connect inner part tube with environment (amniotic cavity) later (by end neurulation) are closed. During brain spinal cord up upper region (up level junction between S1 S2 vertebral bodies), however, this anatomical (sacral-coccygeal division cord, conus medullaris filum terminale) due elongation cavitation cell mass medulla, transforms Thus, main differences that folds invaginates body embryo separates from surface ectoderm, forming an underlying hollow Mesenchymal сlusters form dense undergoes mesenchymal-epithelial transition forms cavities empty during terminal cord. Conclusions. Understanding detailed molecular genetic mechanisms each stage relevant widespread congenital defects, perfect knowledge on aspect all possible factors potential influence it will help develop modern options influencing some them, probably, cause decrease defects.

Language: Английский

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Bioengineering embryo models

Nature Reviews Bioengineering, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 8, 2024

Language: Английский

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