BMP-dependent patterning of ectoderm tissue material properties modulates lateral mesendoderm cell migration during early zebrafish gastrulation DOI Creative Commons
Stefania Tavano, David B. Brückner, Saren Tasciyan

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: July 21, 2023

Summary Cell migration is a fundamental process during embryonic development. Most studies on cell in vivo have focussed the of cells using extracellular matrix (ECM) as their substrate for migration. In contrast, much less known about how migrate other cells, found early embryos when ECM has not yet formed. Here, we show that lateral mesendoderm (LME) zebrafish gastrula use overlying ectoderm We permissive animal pole-directed LME while at pole halts These differences properties are due to being more cohesive and viscous than ectoderm. Consistently, tuning contractility via modulation actomyosin cytoskeleton sufficient change permissiveness Finally, BMP signalling critical reducing cohesion and, thus, its capacity halt towards pole. Collectively, these findings identify role tissue viscosity guiding gastrulation.

Language: Английский

A model for boundary-driven tissue morphogenesis DOI Creative Commons
Daniel S. Alber,

Si-Lu Zhao,

Alexandre O. Jacinto

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: March 11, 2025

Abstract Tissue deformations during morphogenesis can be active, driven by internal processes, or passive, resulting from stresses applied at their boundaries. Here, we introduce the Drosophila hindgut primordium as a model for studying boundary-driven tissue morphogenesis. We characterize its and show that complex shape changes passive consequence of active regions embryo surround it. First, find an intermediate characteristic triangular in 3D hindgut. construct minimal elastic ring deformed midgut invagination germ band extension on ellipsoidal surface, which robustly captures symmetry-breaking into this shape. then quantify kinematics set contours discover deforms two stages: initial translation curved surface followed rapid breaking symmetry. extend our to contour both stages are consistent with picture. Our results suggest role in-plane is translate region anisotropic embryonic curvature uniform boundary conditions sufficient generate observed nonuniform change. work thus provides possible explanation various shapes blastopore-equivalents different organisms framework mechanical emergence global morphologies developmental systems.

Language: Английский

Citations

0
BMP-dependent patterning of ectoderm tissue material properties modulates lateral mesendoderm cell migration during early zebrafish gastrulation DOI Creative Commons
Stefania Tavano, David B. Brückner, Saren Tasciyan

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: July 21, 2023

Summary Cell migration is a fundamental process during embryonic development. Most studies on cell in vivo have focussed the of cells using extracellular matrix (ECM) as their substrate for migration. In contrast, much less known about how migrate other cells, found early embryos when ECM has not yet formed. Here, we show that lateral mesendoderm (LME) zebrafish gastrula use overlying ectoderm We permissive animal pole-directed LME while at pole halts These differences properties are due to being more cohesive and viscous than ectoderm. Consistently, tuning contractility via modulation actomyosin cytoskeleton sufficient change permissiveness Finally, BMP signalling critical reducing cohesion and, thus, its capacity halt towards pole. Collectively, these findings identify role tissue viscosity guiding gastrulation.

Language: Английский

Citations

1