Metaproteomics and metabonomics reveal the metabolic dysfunction of gut microbiota in Tibetan Minipigs in Atherosclerosis DOI Creative Commons
Liye Shen, Jinlong Wang, Yongming Pan

et al.

Research Square (Research Square), Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 28, 2025

Abstract Atherosclerosis is fundamental in the development of cardiovascular disease. The unhealthy dietary habits, high fat and cholesterol intake could change composition gut microbes metabolites which play a critical role atherosclerosis. However, few studies have systematically investigated metabolism In this study, we build an atherosclerosis model using Tibetan minipigs, then identified feces serum, explored functions microbiota by metaproteomics. We found that, feces, multiple signal pathways showed obvious metabolic dysfunction that influence abundance blood products. Several such as 3-dehydro-2-deoxyecdysone from metabolism, leukotriene B4 arachidonic acid indole-3-acetate 3-hydroxyanthranilate tryptophan 9,10-epoxyoctadecenoic linoleic 13(S)-HPOT linolenic were significantly increased blood. These partially increasing associated with inflammation contributes Our finding provide novel clues for studying on mechanism arteriosclerosis.

Language: Английский

Dihydromyricetin Promotes Glucagon‐Like Peptide‐1 Secretion and Improves Insulin Resistance by Modulation of the Gut Microbiota‐CDCA Pathway DOI Creative Commons
Pengfei Li,

Yong Zhang,

Hedong Lang

et al.

Molecular Nutrition & Food Research, Journal Year: 2025, Volume and Issue: unknown

Published: March 13, 2025

ABSTRACT Insulin resistance is a common metabolic disease, and its pathogenesis still unclear. The decrease of glucagon‐like peptide‐1 (GLP‐1) level mediated by the alteration gut microbiota may be pathogenesis. study was to investigate regulatory effect dihydromyricetin (DHM) on GLP‐1 insulin induced high‐fat diet (HFD), further explore possible molecular mechanism. Mice were fed an HFD establish model determine whether DHM had protective effect. could improve resistance. increased serum improving intestinal secretion inhibiting decomposition, associated with intraepithelial lymphocytes (IELs) proportions decreased expression CD26 in IELs TCRαβ + CD8αβ HFD‐induced mice. ameliorate modulation metabolites, particularly regulation chenodeoxycholic acid (CDCA) content, followed inhibition farnesoid X receptor (FXR) L cells glucagon gene (Gcg) mRNA secretion. This research demonstrates role “gut microbiota‐CDCA” pathway improvement levels mice administration, providing new target for prevention

Language: Английский

Citations

1
Gut microbiota-derived 12-ketolithocholic acid suppresses the IL-17A secretion from colonic group 3 innate lymphoid cells to prevent the acute exacerbation of ulcerative colitis DOI Creative Commons
Na Li,

Pei-guang Ma,

Yalan Li

et al.

Gut Microbes, Journal Year: 2023, Volume and Issue: 15(2)

Published: Dec. 8, 2023

Intestinal microbiota dysbiosis and metabolic disruption are well-known as the primary triggers of ulcerative colitis (UC). However, their role in regulating group 3 innate lymphoid cells (ILC3s), which essential for intestinal health, remains unexplored during development disease severity. Here, our results showed that structure patients with severe UC (SUCs) differed from those mild (MiUCs), moderate (MoUCs), healthy controls (HCs). Microbes producing secondary bile acids (SBAs) SBAs decreased aggravation UC, a strong positive correlation existed between them. Next, fecal transfer was used to reproduce human-derived mice decipher microbiota-mediated inflammatory modulation an increase Mice receiving SUC-derived exhibited enhancive inflammation, lowered percentage ILC3s, down-regulated expressions acid receptors, including vitamin D receptor (VDR) pregnane X (PXR), colon. Similar clinical results, SBA-producing microbes, deoxycholic (DCA), 12-ketolithocholic (12-KLCA) were diminished intestine these recipients. Finally, we compared therapeutic potential DCA 12-KLCA preventing regulatory mechanisms mediated by ILC3s. but not represented anti-inflammatory effect associated higher expression VDR lower secretion IL-17A colonic Collectively, findings provide new signatures monitoring acute deterioration targeting gut metabolism demonstrate preventive novel microbiota-derived metabolite, 12-KLCA.

Language: Английский

Citations

20
The gut metabolite 3-hydroxyphenylacetic acid rejuvenates spermatogenic dysfunction in aged mice through GPX4-mediated ferroptosis DOI Creative Commons
Zirun Jin, Yuzhuo Yang, Yalei Cao

et al.

Microbiome, Journal Year: 2023, Volume and Issue: 11(1)

Published: Sept. 27, 2023

Aging-related fertility decline is a prevalent concern globally. Male reproductive system aging mainly characterized by decrease in sperm quality and fertility. While it known that intestinal physiology changes with age microbiota shaped physiology, the underlying mechanism of how affects male still largely unexplored.Here, we utilized fecal transplantation (FMT) to exchange between young old mice. Cecal shotgun metagenomics metabolomics were used identify differences gut composition metabolic regulation during aging. Our results demonstrated FMT from mice alleviated aging-associated spermatogenic dysfunction through an unexpected mediated bacteria-derived metabolite, 3-hydroxyphenylacetic acid (3-HPAA). 3-HPAA treatment resulted improvement spermatogenesis RNA sequencing analysis, qRT-PCR Western blot revealed induced upregulation GPX4, thereby restraining ferroptosis restoring spermatogenesis. These findings further confirmed vitro induction inhibition GPX4 expression.Our demonstrate microbiome-derived 3-HPAA, facilitates ferroptosis-mediated mechanism. Overall, these provide novel dysregulated mice, suggest could be potential therapy for males clinical practice. Video Abstract.

Language: Английский

Citations

19
Effects of dietary arsenic exposure on liver metabolism in mice DOI Creative Commons

Junli Shao,

Chengze Lai,

Qiuyi Zheng

et al.

Ecotoxicology and Environmental Safety, Journal Year: 2024, Volume and Issue: 274, P. 116147 - 116147

Published: March 8, 2024

Arsenic, a ubiquitous environmental toxicant with various forms and complex food matrix interactions, can reportedly exert differential effects on the liver compared to drinking water exposure. To examine its specific liver-related harms, we targeted in C57BL/6 J mice (n=48, 8-week-old) fed arsenic-contaminated (30 mg/kg) for 60 days, mimicking rice arsenic composition observed real-world scenarios (iAsV: 7.3%, iAsIII: 72.7%, MMA: 1.0%, DMA: 19.0%). We then comprehensively evaluated histopathology, metabolic changes, potential role of gut-liver axis using human hepatocellular carcinoma cells (HepG2) microbiota/metabolite analyses. Rice exposure significantly altered hepatic lipid (fatty acids, glycerol lipids, phospholipids, sphingolipids) metabolite (glutathione, thioneine, spermidine, inosine, indole-derivatives, etc.) profiles, disrupting 33 pathways (bile secretion, unsaturated fatty acid biosynthesis, glutathione metabolism, ferroptosis, etc.). Pathological examination revealed cell necrosis/apoptosis, further confirmed by ferroptosis induction HepG2 cells. Gut microbiome analysis showed enrichment pathogenic bacteria linked diseases depletion beneficial strains. Fecal primary secondary bile short-chain branched-chain amino acids were also elevated. Importantly, mediation significant correlations between gut microbiota, fecal metabolites, alterations, suggesting metabolites may mediate impact microbiota disorders. play roles arsenic-induced injuries. Overall, our findings demonstrate that triggers oxidative stress, disrupts induces ferroptosis.

Language: Английский

Citations

8
The Gut Microbiota and Diabetes: Research, Translation, and Clinical Applications—2023 Diabetes, Diabetes Care, and Diabetologia Expert Forum DOI Creative Commons
Mariana X. Byndloss, Suzanne Devkota, Frank A. Duca

et al.

Diabetes Care, Journal Year: 2024, Volume and Issue: unknown

Published: June 24, 2024

This article summarizes the state of science on role gut microbiota (GM) in diabetes from a recent international expert forum organized by Diabetes, Diabetes Care, and Diabetologia, which was held at European Association for Study 2023 Annual Meeting Hamburg, Germany. Forum participants included clinicians basic scientists who are leading investigators field intestinal microbiome metabolism. Their conclusions were as follows: 1) GM may be involved pathophysiology type 2 diabetes, microbially produced metabolites associate both positively negatively with disease, mechanistic links functions (e.g., genes butyrate production) glucose metabolism have recently emerged through use Mendelian randomization humans; 2) highly individualized nature poses major research obstacle, large cohorts deep-sequencing metagenomic approach required robust assessments associations causation; 3) because single-time point sampling misses intraindividual dynamics, future studies repeated measures within individuals needed; 4) much will to determine applicability this expanding knowledge diagnosis treatment, novel technologies improved computational tools important achieve goal.

Language: Английский

Citations

7
The potential influence and intervention measures of gut microbiota on sperm: it is time to focus on testis-gut microbiota axis DOI Creative Commons
Wenkang Chen, Hede Zou, Haoran Xu

et al.

Frontiers in Microbiology, Journal Year: 2024, Volume and Issue: 15

Published: Oct. 8, 2024

As the global male infertility rate continues to rise, there is an urgent imperative investigate underlying causes of sustained deterioration in sperm quality. The gut microbiota emerges as a pivotal factor host health regulation, with mounting evidence highlighting its dual influence on semen. This review underscores interplay between Testis-Gut axis and consequential effects sperm. Potential mechanisms driving impact encompass immune modulation, inflammatory responses mediated by endotoxins, oxidative stress, antioxidant defenses, microbiota-derived metabolites, epigenetic modifications, regulatory sex hormone signaling. Interventions such probiotics, prebiotics, synbiotics, fecal transplantation, Traditional natural herbal extracts are hypothesized rectify dysbiosis, offering avenues modulate enhance Spermatogenesis motility. Future investigations should delve into elucidating foundational principles governing interaction within Axis. Understanding modulating Axis may yield novel therapeutic strategies fertility combat decline

Language: Английский

Citations

6
Association of gastrointestinal microbiome and obesity with gestational diabetes mellitus-an updated globally based review of the high-quality literatures DOI Creative Commons
Jiahui Li, Min Wang, Shuai Ma

et al.

Nutrition and Diabetes, Journal Year: 2024, Volume and Issue: 14(1)

Published: May 21, 2024

The purpose of this review is to investigate the relationship between gastrointestinal microbiome, obesity, and gestational diabetes mellitus (GDM) in an objective manner.

Language: Английский

Citations

5
The gut microbiota and diabetes: research, translation, and clinical applications – 2023 Diabetes, Diabetes Care, and Diabetologia Expert Forum DOI Creative Commons
Mariana X. Byndloss, Suzanne Devkota, Frank A. Duca

et al.

Diabetologia, Journal Year: 2024, Volume and Issue: 67(9), P. 1760 - 1782

Published: June 24, 2024

Abstract This article summarises the state of science on role gut microbiota (GM) in diabetes from a recent international expert forum organised by Diabetes , Care and Diabetologia which was held at European Association for Study 2023 Annual Meeting Hamburg, Germany. Forum participants included clinicians basic scientists who are leading investigators field intestinal microbiome metabolism. Their conclusions were as follows: (1) GM may be involved pathophysiology type 2 diabetes, microbially produced metabolites associate both positively negatively with disease, mechanistic links functions (e.g. genes butyrate production) glucose metabolism have recently emerged through use Mendelian randomisation humans; (2) highly individualised nature poses major research obstacle, large cohorts deep-sequencing metagenomic approach required robust assessments associations causation; (3) because single time point sampling misses intraindividual dynamics, future studies repeated measures within individuals needed; (4) much will to determine applicability this expanding knowledge diagnosis treatment, novel technologies improved computational tools important achieve goal.

Language: Английский

Citations

5
Beyond Auto-Brewery: Why Dysbiosis and the Legalome Matter to Forensic and Legal Psychology DOI Creative Commons

Alan Logan,

Susan L. Prescott, Erica M. LaFata

et al.

Laws, Journal Year: 2024, Volume and Issue: 13(4), P. 46 - 46

Published: July 11, 2024

International studies have linked the consumption of ultra-processed foods with a variety non-communicable diseases. Included in this growing body research is evidence linking to mental disorders, aggression, and antisocial behavior. Although idea that dietary patterns various nutrients or additives can influence brain behavior has long history criminology, absence plausible mechanisms convincing intervention trials, topic was mostly excluded from mainstream discourse. The emergence across nutritional neuroscience psychology/psychiatry, combined mechanistic bench science, human provided support epidemiological findings, legitimacy concept criminology. Among emergent research, microbiome sciences illuminated pathways socioeconomic environmental factors, including foods, aggression Here review, we examine burgeoning related food addiction, explore its relevance criminal justice spectrum—from prevention intervention—and courtroom considerations diminished capacity. We use auto-brewery syndrome as an example intersecting diet gut science been used refute mens rea charges. legalome—microbiome omics applied forensic legal psychology—appears set emerge important consideration matters law, justice.

Language: Английский

Citations

5
The role of glycans in health and disease: Regulators of the interaction between gut microbiota and host immune system DOI Creative Commons
Lucy I. Crouch, Cláudia S. Rodrigues, Cassie R. Bakshani

et al.

Seminars in Immunology, Journal Year: 2024, Volume and Issue: 73, P. 101891 - 101891

Published: May 1, 2024

The human gut microbiota is home to a diverse collection of microorganisms that has co-evolved with the host immune system in which host-microbiota interactions are essential preserve health and homeostasis. Evidence suggests perturbation this symbiotic host-microbiome relationship contributes onset major diseases such as chronic inflammatory including Inflammatory Bowel Disease. glycocalyx (repertoire glycans/sugar-chains at surface mucosa) constitutes biological physical interface between intestinal mucosa microorganisms, well system. Glycans an niche for colonization thus important modulator host-microorganism both homeostasis disease. In review, we discuss role glycome instrumental pathway regulates but also inflammation transition. We power glycosylation remodelling attractive preventive therapeutic strategy

Language: Английский

Citations

5