Strain- and Subtype-Specific Replication of Genotype 3 Hepatitis E Viruses in Mongolian Gerbils DOI Creative Commons
Tian‐Cheng Li, Yusuke Sakai, Yasushi Ami

et al.

Viruses, Journal Year: 2024, Volume and Issue: 16(10), P. 1605 - 1605

Published: Oct. 12, 2024

Since Mongolian gerbils are broadly susceptible to hepatitis E virus (HEV), including genotypes 1, 4, 5, and 8 (HEV-1, HEV-5, HEV-8) rat HEV, they a useful small animal model for HEV. However, we have observed that the subtypes HEV-3k HEV-3ra in genotype 3 HEV (HEV-3) were not infected efficiently gerbils. A small-animal HEV-3 is also needed since responsible major zoonotic infections. To investigate whether can be used as models other of HEV-3, injected with five (HEV-3b, -3e, -3f, -3k, -3ra) compared infectivity subtypes. We detected viral RNA gerbils' feces. High titers anti-HEV IgG antibodies serum induced all HEV-3b/ch-, HEV-3f-, HEV-3e-injected Especially, animals released high levels viruses into their feces an extended period. The replication was limited HEV-3b/wb-injected HEV-3k-injected groups. Although HEV-3ra-injected gerbils, copy numbers fecal specimens low; no sera. These results indicate although HEV-3's depends on subtype strain, potential HEV-3. further comparison HEV-3e different strains (HEV-4i HEV-5) genera (rat HEV) revealed ALT elevations among strains, liver damage occurred HEV-4i- HEV-5-infected but HEV-3e- or HEV-infected demonstrating variable pathogenicity across HEVs from might candidate examine HEV's pathogenicity.

Language: Английский

Distinct Disruptive Effects of Methylparaben and Propylparaben on the Prostate and Gonads of Adult Gerbils DOI Open Access

Edvaldo Mendes Silva,

Janaína Ribeiro Costa,

A. A. Correa

et al.

Environmental Toxicology, Journal Year: 2025, Volume and Issue: unknown

Published: March 4, 2025

ABSTRACT Parabens are chemicals used as preservatives in food, personal care products, and pharmaceuticals. Detectable paraben levels found water, air, soil, making human animal exposure unavoidable. This study evaluated the effects of methylparaben, propylparaben, their mixture on prostate gonads male female gerbils. Gerbils aged 90 days were assigned to groups: (M) 10 mg/kg/day methylparaben; (P) propylparaben; (M + P) 5 methylparaben plus (C) control group. The animals treated orally for 30 consecutive days, prostates subjected morphometric‐stereological, immunohistochemical, biochemical analyses. ventral P M groups showed an increase relative weight, higher cell proliferation, besides occurrence hyperplasia epithelial desquamation. All treatments reduced malondialdehyde prostate. exhibited alterations all groups, but with greater severity groups. These included increased proliferation hyperplasia, well stromal inflammatory foci secretory activity. M, P, diameter seminiferous tubules, thickness germinal epithelium, catalase activity testes. In ovaries, primary follicle number superoxide dismutase results indicate that propylparaben is more harmful than acceptable daily intake these parabens not safe reproductive health.

Language: Английский

Citations

0
Burden of Acute Hepatitis E Virus in South Asia: Insights from Global Burden of Disease Study 2021 DOI

Prakasini Satapathy,

Shilpa Gaidhane,

Ashok Kumar Bishoyi

et al.

Diagnostic Microbiology and Infectious Disease, Journal Year: 2025, Volume and Issue: 112(3), P. 116767 - 116767

Published: March 8, 2025

Language: Английский

Citations

0
Molecular characterization of autochthonous Hepatitis E virus detected from a human acute infection in the Calabria Region, Southern Italy DOI
Nadia Marascio,

Marta Pantanella,

Grazia Pavia

et al.

Diagnostic Microbiology and Infectious Disease, Journal Year: 2025, Volume and Issue: unknown, P. 116807 - 116807

Published: March 1, 2025

Language: Английский

Citations

0
Distinct Distribution of HEV-3 Subtypes across Humans, Animals, and Environmental Waters in Sweden DOI Creative Commons
Hao Wang, Marianela Patzi Churqui,

Samaneh Taslimi

et al.

Emerging Microbes & Infections, Journal Year: 2025, Volume and Issue: unknown

Published: April 1, 2025

We previously observed a notable discrepancy in the distribution of HEV-3 subtypes between wastewater and clinical samples Sweden. To confirm this observation comprehensively elucidate circulation patterns across humans, animals, environmental waters Sweden, we analyzed HEV genetic diversity archived late 2016 early 2018, cases 2012 2024, all available Swedish sequences from NCBI Virus database. RNA was detected samples, with subtype 3c being only identified. In typed cases, 3f (45/126) (44/126) were nearly equally distributed, though regional dominance varied. When incorporating human other studies, became dominant (75/168). Analysis revealed that (113/136) Sus scrofa (pigs wild boars), while (30/33) waters. These findings highlight complex transmission dynamics The near-absence domestic pigs boars, despite its high proportion raises question about source infection. addition, near-exclusive detection suggests potential differences viral shedding, disease severity subtypes, or alternative host sources. This study emphasizes importance integrated One Health surveillance to track reservoirs.

Language: Английский

Citations

0
Performance of sofosbuvir and NITD008 in extrahepatic neuronal cells against HEV DOI Creative Commons
Michelle Jagst,

André Gömer,

Daniel Tödt

et al.

Antiviral Research, Journal Year: 2024, Volume and Issue: 227, P. 105922 - 105922

Published: May 31, 2024

Hepatitis E is an underestimated disease, leading to estimated 20 million infections and up 70,000 deaths annually. Infections are mostly asymptomatic, but can reach mortality rates 25% in pregnant women or become chronic immunocompromised patients. virus (HEV) infection have been associated with a range of extrahepatic manifestations, including spectrum neurological symptoms. Current therapy options limited non-specific antivirals like ribavirin, recently, repurposed viral polymerase inhibitors sofosbuvir NITD008 were described inhibit HEV replication. Here, we evaluated the efficacy these drugs various neuronal-derived cell lines determine their potency outside liver. Our findings indicate that both drugs, especially sofosbuvir, exhibited reduced neuronal cells compared hepatic cells. These results should be taken into account development direct-acting for at replication sites.

Language: Английский

Citations

3
Epidemiological study of hepatitis E virus infection among students and workers in Hebei Province of China DOI
Huan Liu,

Kunjing Geng,

Chunyan Wang

et al.

Zoonoses and Public Health, Journal Year: 2024, Volume and Issue: unknown

Published: May 20, 2024

Hepatitis E caused by the hepatitis virus (HEV) is prevalent worldwide. In China, considerable shifts in epidemiology of have been observed over last two decades, with ongoing changes prevalence HEV.

Language: Английский

Citations

2
Active surveillance of hepatitis E: a 10-year epidemiological analysis in a city in eastern China DOI Creative Commons
Xingcheng Huang, Xiaoxiang Zheng, Chen Lin

et al.

Emerging Microbes & Infections, Journal Year: 2024, Volume and Issue: 13(1)

Published: June 26, 2024

Hepatitis E virus (HEV) is an important cause of acute hepatitis, however, highly neglected and largely underreported. This study aimed to describe the detailed epidemiology hepatitis (HE) through a 10-year surveillance. A community-based active surveillance was conducted between November 2007 October 2017 in 11 townships Dongtai City China, involving 355,673 residents. Serum samples were obtained from patients presenting with symptoms for more than 3 days. alanine aminotransferase (ALT) levels greater 2.5 times upper limit normal (ULN) considered hepatitis. Samples subsequently tested IgG IgM anti-HEV antibodies, HEV RNA, B surface antigen (HBsAg). The data indicated incidence HE fluctuated downward 2017, average annual age-standardized 17.50 per 100,000, exceeding 10.26 100,000 National Notifiable Disease Report System (NNDRS). notably higher among males (20.95 100,000) individuals aged 50-69 years (37.47 100,000). Genotype 4 (HEV-4) predominantly circulating genotype during period. Furthermore, revealed (HBV) co-infection 4.99 100,000. system identified compared NNDRS, decreased prevalence over While efforts are still needed prevent high-risk populations, including elderly.

Language: Английский

Citations

2
Optimization of immunosuppression strategies for the establishment of chronic hepatitis E virus infection in rabbits DOI
Qiyu He, Tianxu Liu, Xinyue Yang

et al.

Journal of Virology, Journal Year: 2024, Volume and Issue: 98(7)

Published: July 23, 2024

ABSTRACT Chronic hepatitis E mostly occurs in organ transplant recipients and can lead to rapid liver fibrosis cirrhosis. Previous studies found that the development of chronic virus (HEV) infection is linked type immunosuppressant used. Animal models are crucial for study pathogenesis E. We previously established a stable HEV rabbit model using cyclosporine A (CsA), calcineurin inhibitor (CNI)-based immunosuppressant. However, immunosuppression strategy timing may be optimized, how different types immunosuppressants affect establishment this still unknown. Here, we showed 100% rabbits when CsA treatment was started at challenge or even 4 weeks after. Tacrolimus prednisolone alone also contributed infection, resulting 77.8% chronicity rates, respectively, while mycophenolate mofetil (MMF) only led 28.6% rate. accompanied with persistent activation innate immune response evidenced by transcriptome analysis. The suppressed adaptive low expression genes related cytotoxicity (like perforin FasL ) anti-HEV seroconversion rates play important roles causing infection. By analyzing antigen concentrations outcomes, levels could indicate development. This optimized strategies establishing highlighted potential association between immunosuppressants. IMPORTANCE Organ high risk generally receive CNI-based regimen containing CNI (tacrolimus CsA), MMF, and/or corticosteroids. Previously, before challenge. In study, further rabbits. same time after challenge, clearly indicating progression under these circumstances necessity screening both recipient donor preoperatively. CsA, tacrolimus, instead MMF significantly acute phase indicates Our results have implications understanding

Language: Английский

Citations

1
Ancient evolutionary origins of hepatitis E virus in rodents DOI
Wendy K. Jo, Murilo Henrique Anzolini Cassiano, Edmilson Ferreira de Oliveira-Filho

et al.

Proceedings of the National Academy of Sciences, Journal Year: 2024, Volume and Issue: 121(51)

Published: Dec. 9, 2024

Hepatitis E virus (HEV; family

Language: Английский

Citations

1
One‐Pot Assay Based on CRISPR/Cas13a Technology for HEV RNA Point‐of‐Care Testing DOI Creative Commons
Zihao Fan, Ling Xu, Yaling Cao

et al.

Journal of Medical Virology, Journal Year: 2024, Volume and Issue: 96(12)

Published: Dec. 1, 2024

ABSTRACT Hepatitis E virus (HEV) poses a serious threat to both public health and animal food safety, thereby highlighting the demands for rapid, sensitive, easy‐to‐use detection. This study aimed develop One‐Pot assay using CRISPR/Cas13a detecting HEV RNA, suitable point‐of‐care testing (POCT) in resource‐limited settings. combined with reverse transcription polymerase chain reaction (RT‐PCR) recombinase‐aided amplification (RT‐RAA) was applied device. Additionally, large cohort of HEV‐infected patient (154) (104) specimens utilized validation. The RT‐PCR/RT‐RAA + assays RNA detection (genotypes: HEV‐1, HEV‐3, HEV‐4) were established, optimized, validated, achieving limit (LoD) 1 copy/μL 100% specificity. In application validation infection, positive rates RT‐PCR CRISPR RT‐RAA 98.6% 89.6% patients, 96.6% 88.8% animals, respectively, which superior those RT‐qPCR. Furthermore, sample rapid lysis, reagent lyophilization, device integrated construct an LoD 10 2 copies/μL. Despite slight decreases sensitivity, significantly reduces time 35 min, making it easy perform, minimizing contamination, meeting requirements screening. We developed effectively realizes POCT test maximizes impetus implementation shows potential as valuable tool monitoring infection.

Language: Английский

Citations

1