Current and Potential Use of Biologically Active Compounds Derived from Cannabis sativa L. in the Treatment of Selected Diseases

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(23), P. 12738 - 12738

Published: Nov. 27, 2024

Cannabis sativa L. contains numerous compounds with antioxidant and anti-inflammatory properties, including the flavonoids cannabinoids, particularly Δ-9-tetrahydrocannabinol (THC) cannabidiol (CBD). Cannabinoids have an effect on endocannabinoid system (ECS), a cellular communication network, are, hence, widely studied for medical applications. Epidiolex®, 99% pure oral CBD extract, has been approved by FDA treatment of epilepsy. Nabiximols (Sativex) is oromucosal spray containing equal volume THC CBD, it commonly used as add-on unresponsive spasticity in multiple sclerosis (MS) patients. Several vitro vivo studies also shown that cannabinoids can be to treat various types cancer, such melanoma brain glioblastoma; first positive clinical trials anticancer THC:CBD blend temozolomide (TMZ) highly invasive cancer are very promising. The exert their properties investigations induction cell death, mainly apoptosis cytotoxic autophagy, inhibition proliferation. In several studies, found induce tumor regression inhibit angiogenic mechanisms vivo, well two low-numbered epidemiological studies. They exhibit antiviral effects inhibiting ACE2 transcription, blocking viral replication fusion, acting agents; indeed, prior consumption (a study 93,565 persons Chicago) associated much lower incidence SARS-CoV-2 infections. It postulated cannabis extracts many other diseases systemic lupus erythematosus, type 1 diabetes, or neurological disorders, e.g., Alzheimer’s disease. aim this review outline current state knowledge regarding currently medicinal preparations derived from C. selected diseases, present latest research potential applications its secondary metabolites.

Language: Английский

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An Unexpected Activity of a Minor Cannabinoid: Cannabicyclol (CBL) Is a Potent Positive Allosteric Modulator of Serotonin 5-HT_1A Receptor

Journal of Natural Products, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 15, 2025

Cannabicyclol ((±)-CBL), a minor phytocannabinoid, is largely unexplored, with its biological activity previously undocumented. We studied conversion from cannabichromene (CBC) using various acidic catalysts. Montmorillonite (K30) in chloroform at room temperature had the highest yield (60%) minimal byproducts. Key reaction conditions, such as solvent, temperature, and time, significantly impacted yield. The structure of (±)-CBL was confirmed via X-ray crystallography. Stability studies showed that MCT oil dilution remain stable 25–40 °C for three months. Radioligand binding assays revealed high affinity CBL 5-HT1A receptor but weak interaction CB1 CB2 receptors. At 10 μM 1 μM, inhibited [3H]-8-hydroxy-DPAT to by 75% 20%, respectively. Functional acts agonist concentrations potent positive allosteric modulator serotonin-induced activation low concentrations. 4 increased β-arrestin recruitment 20% 80%. This unique modulatory profile highlights potential drug discovery targeting serotonin

Language: Английский

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Antimicrobial Potential of Cannabinoids: A Scoping Review of the Past 5 Years

Microorganisms, Journal Year: 2025, Volume and Issue: 13(2), P. 325 - 325

Published: Feb. 2, 2025

In the scenario of fighting bacterial resistance to antibiotics, natural products have been extensively investigated for their potential antibacterial activities. Among these, cannabinoids—bioactive compounds derived from cannabis—have garnered attention diverse biological activities, including anxiolytic, anti-inflammatory, analgesic, antioxidant, and neuroprotective properties. Emerging evidence suggests that cannabinoids may also possess significant antimicrobial properties, with applications in enhancing efficacy conventional agents. Therefore, this review examines past five years on properties cannabinoids, focusing underlying mechanisms such as microbial membrane disruption, immune response modulation, interference virulence factors. addition, synergistic potential, when used alongside standard therapies, underscores promise a novel strategy address drug resistance, although further research clinical trials are needed validate therapeutic use. Overall, offer promising avenue development innovative treatments combat drug-resistant infections reduce reliance traditional

Language: Английский

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Anti-Infective Screening of Selected Nine Cannabinoids Against Clostridium perfringens and Influenza A (H5N1) Neuraminidases, and SARS-CoV-2 Main Protease and Spike Protein Interactions

Current Issues in Molecular Biology, Journal Year: 2025, Volume and Issue: 47(3), P. 185 - 185

Published: March 12, 2025

Recently, cannabinoids have gained scientific interest as a promising anti-infective natural product class, reported in several studies. However, the existing knowledge is mainly limited to common like THC and CBD. Therefore, this study aims fill gap by investigating potential of nine selected (both rare cannabinoids): THC, CBD, CBC, CBE, CBF, CBG, CBL, CBN, CBT against Clostridium perfringens Influenza A (H5N1) neuraminidases SARS-CoV-2 main protease spike protein–human ACE2 interaction using standard vitro biochemical enzyme-binding assay. As result, authors’ knowledge, first demonstrate most effect CBG over others its class C. influenza interaction. In comparison CBD were second third candidates. Meanwhile, other derivatives, such CBT, showed at least one effect. Our findings during early drug discovery process indicate cannabinoids, which can be considered for further investigation biological setup.

Language: Английский

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Aescin Inhibits Herpes simplex Virus Type 1 Induced Membrane Fusion

Planta Medica, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 23, 2024

Abstract Infections with Herpes simplex virus can cause severe ocular diseases and encephalitis. The present study aimed to investigate potential inhibitors of fusion between HSV-1 the cellular membrane host cell. Fusion entry into cell is mimicked by a virus-free eukaryotic culture system co-expression glycoproteins gD, gH, gL, gB in presence gD receptor, resulting excessive polykaryocyte formation. A microscopic read-out was used for screening inhibitors, whereas luminometric quantification cell-cell reporter assay. tagged at its C-terminus mCherry express mCherry-gB both assay systems visualization Reporter protein expression SEAP regulated Tet-On 3 G system. saponin mixture aescin identified as specific inhibitor (IC50 7.4 µM, CC50 24.3 SI 3.3) fusion. plaque reduction on Vero cells reduced cell-to-cell spread significantly; 15 µM decreased relative counts 41%, 10 resulted residual size 11% (HSV-1 17 syn+) 2% ANG path). Release progeny one log step aescin. Virus particle integrity mainly unaffected. Analytical investigation UHPLC-MS revealed IA -IB isoaescin main compounds different functional activities. Aescin had lowest IC50, highest CC50, an > 4.6.

Language: Английский

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Antiviral Activity Against Hsv-1 of Triterpene Saponins from Anagallis Arvensis Is Related to the Fusion-Inhibitory Activity of Desglucoanagalloside B

Published: Jan. 1, 2024

Language: Английский

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