Direct identification of the calcium-binding amino acid, gamma-carboxyglutamate, in mineralized tissue.

Proceedings of the National Academy of Sciences, Journal Year: 1975, Volume and Issue: 72(10), P. 3925 - 3929

Published: Oct. 1, 1975

A direct approach has been developed for quantitative identification of the calcium-binding amino acid, gamma-carboxyglutamate, in proteins. This should be advantageous study numerous systems where specific roles binding calcium or other divalent cations are suspected. Investigation mineralized tissue, proteins implicated mineralization process, revealed that gamma-carboxyglutamate was present solubilized from chicken bone with neutral aqueous ethylenediamine tetraacetic acid. established by isolation acid alkaline hydrolysates and its conversion to glutamic decarboxylation 0.05 M HCl at 100 degrees. The kinetics chromatographic behavior identical those human prothrombin. After resolution soluble phosphate gradient elution hydroxyapatite, found concentrated primarily one BaSO4-adsorbable anionic protein species; collagen devoid In view recently discovered requirement vitamin K generation sites (gamma-carboxyglutamate) gamma-carboxylation residues prothrombin, our findings may implicate metabolism normal development suggest a role gamma-carboxyglutamate-rich regulation salt deposition tissues.

Language: Английский

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Platelets and cancer: a casual or causal relationship: revisited

Cancer and Metastasis Reviews, Journal Year: 2014, Volume and Issue: 33(1), P. 231 - 269

Published: March 1, 2014

Language: Английский

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Separation, characterization and identification of microplastics and nanoplastics in the environment

The Science of The Total Environment, Journal Year: 2020, Volume and Issue: 721, P. 137561 - 137561

Published: March 2, 2020

Language: Английский

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Effect of Osocimab in Preventing Venous Thromboembolism Among Patients Undergoing Knee Arthroplasty

JAMA, Journal Year: 2020, Volume and Issue: 323(2), P. 130 - 130

Published: Jan. 14, 2020

Importance

The efficacy of factor XIa inhibition for thromboprophylaxis is unknown. Osocimab a long-acting, fully human monoclonal antibody that inhibits XIa.

Objective

To compare different doses osocimab with enoxaparin and apixaban in patients who have undergone knee arthroplasty.

Design, Setting, Participants

Randomized, open-label, adjudicator-blinded, phase 2 noninferiority trial observer blinding doses, conducted at 54 hospitals 13 countries. Adult undergoing unilateral arthroplasty were randomized from October 2017 through August 2018 followed up until January 2019.

Interventions

Single intravenous postoperative 0.3 mg/kg (n = 107), 0.6 65), 1.2 108), or 1.8 106); preoperative 109) 108); 40 mg subcutaneous once daily 105) 2.5 oral twice least 10 days venography.

Main Outcomes Measures

primary outcome was venous thromboembolism incidence between postoperatively (assessed by mandatory bilateral venography performed to after surgery confirmed symptomatic deep vein thrombosis pulmonary embolism). A 5% margin compared chosen. safety major clinically relevant nonmajor bleeding assessed postoperatively.

Results

Of 813 participants (mean [SD] age, 66.5 years [8.2 years]; body mass index, 32.7 [5.7]; 74.2% women), 600 included the per-protocol population used analysis. occurred 18 (23.7%) receiving mg/kg, 8 (15.7%) (16.5%) 14 (17.9%) postoperatively; 23 (29.9%) 9 (11.3%) preoperatively; 20 (26.3%) enoxaparin; 12 (14.5%) apixaban. given met criteria risk differences (1-sided 95% CIs) 10.6% (95% CI, –1.2% ∞) 0.6-mg/kg dose; 9.9% –0.9% 1.2-mg/kg dose, 8.4% –2.6 1.8-mg/kg dose. dose superiority difference 15.1%; 2-sided 90% 4.9% 25.2%). Postoperative did not meet prespecified noninferiority, 2.6% –8.9% –3.6% –15.5% ∞), respectively. Major observed 4.7% those osocimab, 5.9% enoxaparin, 2%

Conclusions Relevance

Among arthroplasty, Further studies are needed establish relative standard thromboprophylaxis.

Trial Registration

ClinicalTrials.gov Identifier:NCT03276143

Language: Английский

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Bleeding, thrombosis, and anticoagulation in myeloproliferative neoplasms (MPN): analysis from the German SAL-MPN-registry

Journal of Hematology & Oncology, Journal Year: 2016, Volume and Issue: 9(1)

Published: March 4, 2016

Patients with Ph-negative myeloproliferative neoplasms (MPN), such as polycythemia vera (PV), essential thrombocythemia (ET), and primary myelofibrosis (PMF), are at increased risk for thrombosis/thromboembolism major bleeding. Due to the morbidity mortality of these events, antiplatelet and/or anticoagulant agents commonly employed secondary prophylaxis. On other hand, disease-related bleeding complications (i.e., from esophageal varices) common in patients MPN. This analysis was performed define frequency identify factors, assess antiplatelet/anticoagulant therapy a cohort The MPN registry Study Alliance Leukemia is non-interventional prospective study including adult an according WHO criteria (2008). For statistical analysis, descriptive methods tests significant differences well contingency tables were used odds potential factors vascular events. subgroups significantly differed sex distribution, age diagnosis, blood counts, LDH levels, JAK2V617F positivity, spleen size (length). While most thromboembolic events occurred around time one third after that date. Splanchnic vein thrombosis frequent post-PV-MF MPN-U patients. chance developing event elevated if suffered (OR 3.43; 95 % CI = 1.39–8.48) splenomegaly 1.76; 1.15–2.71). Significant previous 2.71; 1.36–5.40), 2.22; 1.01–4.89), administration heparin 5.64; 1.84–17.34). Major episodes less ET compared subgroups. Together, this report on unselected "real-world" German reveals important data prevalence, treatment

Language: Английский

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Biomaterials and biocompatibility: An historical overview

Journal of Biomedical Materials Research Part A, Journal Year: 2020, Volume and Issue: 108(8), P. 1617 - 1633

Published: March 20, 2020

This review focuses on the characteristics and applications of biomaterials through ages, ranging from prehistoric times to beginning era modern medicine, which has been arbitrarily set middle 19th century, when aseptic procedures, antiseptic substances anesthetics were developed. After a brief discussion definition "biomaterial" an historical point view short introduction general history surgery dentistry, each material or class materials will be presented with references listed in chronological order and, where possible, their real, scientifically demonstrated effects biological tissues. Particular attention given that are nowadays considered spurious affected by translation errors other kinds biases.

Language: Английский

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Follicular plasminogen and plasminogen activator and the effect of plasmin on ovarian follicle wall

Cell, Journal Year: 1975, Volume and Issue: 6(3), P. 379 - 386

Published: Nov. 1, 1975

Language: Английский

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Sulfation, anticoagulant and antioxidant activities of polysaccharide from green algae Enteromorpha linza

International Journal of Biological Macromolecules, Journal Year: 2013, Volume and Issue: 58, P. 225 - 230

Published: April 16, 2013

Language: Английский

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SARS-CoV-2 cell entry beyond the ACE2 receptor

Molecular Biology Reports, Journal Year: 2022, Volume and Issue: 49(11), P. 10715 - 10727

Published: June 26, 2022

Language: Английский

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Antibody-Dependent Enhancement (ADE) and the role of complement system in disease pathogenesis

Molecular Immunology, Journal Year: 2022, Volume and Issue: 152, P. 172 - 182

Published: Nov. 10, 2022

Antibody-dependent enhancement (ADE) has been associated with severe disease outcomes in several viral infections, including respiratory infections. In vitro and vivo studies showed that antibody-response to SARS-CoV MERS-CoV could exacerbate infection via ADE. Recently SARS CoV-2, the structural analysis shows a risk of severity This phenomenon is partially attributed non-neutralizing antibodies or at sub-neutralizing levels. These result antigen-antibody complexes' deposition propagation chronic inflammatory process destroys affected tissues. Further, complexes may enhance internalization virus into cells through Fc gamma receptor (FcγR) lead further replication. Thus, ADE occur two mechanisms; 1. Antibody mediated replication 2. Enhanced immune activation. Antibody-mediated effector functions are mainly driven by complement activation, first cascade 1q (C1q) which binds virus-antibody complex. Reports say deficiency circulating plasma levels C1q, an independent predictor mortality high-risk patients, diabetes, Complement reported infections such as dengue, West Nile virus, measles, RSV, Human immunodeficiency (HIV), Ebola virus. review discusses evidence coronaviruses. We outline mechanisms ADE, emphasizing role complements, especially C1q outcome enhanced disease.

Language: Английский

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