International Journal of Molecular Sciences,
Journal Year:
2023,
Volume and Issue:
24(19), P. 14722 - 14722
Published: Sept. 29, 2023
Cellular
asymmetry
is
an
important
element
of
efficiency
in
the
compartmentalization
intracellular
chemical
reactions
that
ensure
efficient
tissue
function.
Improving
current
3D
printing
methods
by
using
cellular
essential
producing
complex
tissues
and
organs
such
as
liver.
The
use
cell
spots
containing
at
least
two
cells
basement
membrane-like
bio
support
materials
allows
to
be
tethered
points
on
membrane
with
another
order
maintain
asymmetry.
Our
model
a
new
type
bioprinter
uses
oriented
multicellular
complexes
This
novel
approach
necessary
replace
sequential
slow
processes
organogenesis
rapid
growth
organ
printing.
extracellular
matrix
process
bioprinting
one
preserve
thus
biological
metabolic
efficiency.
Science Advances,
Journal Year:
2023,
Volume and Issue:
9(3)
Published: Jan. 20, 2023
Spatiotemporally
coordinated
transformations
in
epithelial
curvature
are
necessary
to
generate
crypt-villus
structures
during
intestinal
development.
However,
the
temporal
regulation
of
mechanotransduction
pathways
that
drive
crypt
morphogenesis
remains
understudied.
Intestinal
organoids
have
proven
useful
study
vitro,
yet
reliance
on
static
culture
scaffolds
limits
ability
assess
effects
changing
curvature.
Here,
a
photoinduced
hydrogel
cross-link
exchange
reaction
is
used
spatiotemporally
alter
and
how
dynamic
changes
influence
instruct
morphogenesis.
Photopatterned
increased
membrane
tension
depolarization,
which
was
required
for
subsequent
nuclear
localization
yes-associated
protein
1
(YAP)
observed
24
hours
following
change.
Curvature-directed
only
occurred
delay
induction
differentiation
coincided
with
spatially
restricted
YAP
localization,
indicating
initiate
curvature–dependent
temporally
regulate
Communications Biology,
Journal Year:
2025,
Volume and Issue:
8(1)
Published: Feb. 20, 2025
Abstract
Cell
morphology
heterogeneity
is
pervasive
in
epithelial
collectives,
yet
the
underlying
mechanisms
driving
such
and
its
consequential
biological
ramifications
remain
elusive.
Here,
we
observed
a
consistent
correlation
between
cell
nucleus
during
crowding,
revealing
persistent
log-normal
probability
distribution
characterizing
both
areas
across
diverse
model
systems.
We
showed
that
this
morphological
diversity
arises
from
asymmetric
partitioning
division.
Next,
provide
insights
into
impact
of
on
chromatin
modifications.
demonstrated
constraining
leads
to
downregulation
euchromatic
mark
H3K9ac
upregulation
heterochromatic
H3K27me3.
Furthermore,
size
regulates
H3K27me3
levels
through
histone
demethylase
UTX.
These
findings
highlight
significance
as
driver
state
diversity,
shaping
functional
variability
within
tissues.
Mechanobiology in Medicine,
Journal Year:
2024,
Volume and Issue:
2(3), P. 100068 - 100068
Published: April 27, 2024
Dendritic
cells
(DCs)
play
a
pivotal
role
in
bridging
the
innate
and
adaptive
immune
systems.
From
their
immature
state,
scavenging
tissue
for
foreign
antigens
to
uptake,
then
maturation,
trafficking
lymph
nodes
antigen
presentation,
these
are
exposed
various
forms
of
mechanical
forces.
Particularly,
tumor
microenvironment,
it
is
widely
known
that
microenvironmental
biomechanical
cues
heightened.
The
source
forces
arises
from
cell-to-extracellular
matrix
(ECM)
cell-to-cell
interactions,
as
well
being
increased
pressures
fluid
shear
typical
tumors.
DCs
integrate
forces,
influencing
functions
through
mechanotransduction.
This
aspect
DC
biology
holds
alternative,
but
important
clues
understanding
suppressed/altered
responses
tumors,
or
allow
artificial
enhancement
therapeutic
purposes.
review
discusses
current
mechanobiology
perspectives
sensors
providers
International Journal of Molecular Sciences,
Journal Year:
2024,
Volume and Issue:
25(11), P. 5980 - 5980
Published: May 30, 2024
Pinin
(PNN)
is
a
desmosome-associated
protein
that
reinforces
the
organization
of
keratin
intermediate
filaments
and
stabilizes
anchoring
cytoskeleton
network
to
lateral
surface
plasma
membrane.
The
aberrant
expression
PNN
affects
strength
cell
adhesion
as
well
modifies
intracellular
signal
transduction
pathways
leading
onset
CRC.
In
our
previous
studies,
we
characterized
role
miR-195-5p
in
regulation
desmosome
junctions
CRC
progression.
Here,
with
aim
investigating
additional
mechanisms
related
complex,
identified
putative
target.
Using
public
data
repository,
found
was
negative
prognostic
factor
overexpressed
colon
cancer
tissues
from
stage
1
disease.
Then,
assessed
tissue
specimens,
confirming
overexpression
tumor
sections.
increase
levels
revealed
significant
decrease
at
mRNA
levels.
As
consequence
by
miR-195-5p,
KRT8
KRT19,
closely
connected
PNN,
affected.
Finally,
investigated
vivo
effect
on
AOM/DSS-treated
mice.
conclusion,
have
new
mechanism
driven
components,
suggesting
potential
pharmacological
target
for
therapy.
iScience,
Journal Year:
2023,
Volume and Issue:
26(5), P. 106583 - 106583
Published: April 7, 2023
Cell
remodeling
relies
on
dynamic
rearrangements
of
cell
contacts
powered
by
the
actin
cytoskeleton.
The
tumor
suppressor
adenomatous
polyposis
coli
(APC)
nucleate
filaments
(F-actin)
and
localizes
at
junctions.
Whether
APC-driven
nucleation
acts
in
junction
remains
unknown.
By
combining
bioimaging
genetic
tools
with
artificial
intelligence
algorithms
applied
to
colorectal
cancer
cell,
we
found
that
APC-dependent
pool
contributes
sustaining
levels
F-actin,
as
well
E-cadherin
occludin
protein
Moreover,
this
activity
preserved
length
angle,
vertex
motion
integrity.
Loss
F-actin
led
larger
cells
slow
random
movement
within
a
sheet.
Our
findings
suggest
promotes
integrity
dynamics
facilitate
collective
motility.
This
offers
new
perspective
explore
relevance
cytoskeletal
function
gut
morphogenesis.
Experimental Cell Research,
Journal Year:
2022,
Volume and Issue:
417(1), P. 113204 - 113204
Published: May 16, 2022
Muscle
stem
cells
(MuSCs)
are
involved
in
muscle
maintenance
and
regeneration.
Mechanically
loaded
MuSCs
within
their
native
niche
undergo
tensile
shear
deformations,
but
how
sense
mechanical
stimuli
translate
these
into
biochemical
signals
regulating
function
fate
is
still
poorly
understood.
We
aimed
to
investigate
whether
the
glycocalyx
MuSC
mechanoresponse,
morphology
affects
loading-induced
pressure,
stress,
fluid
velocity
distribution.
FSS-induced
deformation
of
active
proliferating
(myoblasts)
with
intact
or
degraded
was
assessed
by
live-cell
imaging.
Glycocalyx-degradation
did
not
significantly
affect
nitric
oxide
production,
reduced
myoblast
modulated
gene
expression.
Finite-element
analysis
revealed
that
distribution
on
myoblasts
non-uniform,
magnitude
depended
apex-height.
In
conclusion,
our
results
suggest
does
play
a
role
NO
production
might
impact
mechanotransduction
expression,
which
needs
further
investigation.
Future
studies
will
unravel
underlying
mechanism
deformation,
be
related
increased
drag
forces.
Moreover,
varying
apex-height
experience
different
levels
velocity,
suggesting
differential
responsiveness
