Strategies to overcome the limitations of current organoid technology - engineered organoids DOI Creative Commons

Xulong Fan,

Kun Hou,

Gaojian Liu

et al.

Journal of Tissue Engineering, Journal Year: 2025, Volume and Issue: 16

Published: April 1, 2025

Organoids, as 3D in vitro models derived from stem cells, have unparalleled advantages over traditional cell and animal for studying organogenesis, disease mechanisms, drug screening, personalized diagnosis treatment. Despite the tremendous progress made organoid technology, translational application of organoids still presents enormous challenges due to complex structure function human organs. In this review, limitations technologies are first described. Next, we explore ways address many cultures by engineering various dimensions systems. Finally, discuss future directions field, including potential roles simulated microphysiology system We hope that review inspires research into system.

Language: Английский

The microbiota–gut–brain axis and neurodevelopmental disorders DOI Creative Commons
Qinwen Wang,

Qianyue Yang,

Xingyin Liu

et al.

Protein & Cell, Journal Year: 2023, Volume and Issue: 14(10), P. 762 - 775

Published: May 11, 2023

Abstract The gut microbiota has been found to interact with the brain through microbiota–gut–brain axis, regulating various physiological processes. In recent years, impacts of on neurodevelopment this axis have increasingly appreciated. is commonly considered regulate three pathways, immune pathway, neuronal and endocrine/systemic overlaps crosstalks in between. Accumulating studies identified role neurodevelopmental disorders including autism spectrum disorder, attention deficit hyperactivity Rett Syndrome. Numerous researchers examined pathophysiological mechanisms influenced by (NDDs). This review aims provide a comprehensive overview advancements research pertaining microbiota-gut-brain NDDs. Furthermore, we analyzed both current state progress discuss future perspectives field.

Language: Английский

Citations

111
Patterning and folding of intestinal villi by active mesenchymal dewetting DOI Creative Commons
Tyler R. Huycke, Teemu J. Häkkinen,

Hikaru Miyazaki

et al.

Cell, Journal Year: 2024, Volume and Issue: 187(12), P. 3072 - 3089.e20

Published: May 22, 2024

Tissue folds are structural motifs critical to organ function. In the intestine, bending of a flat epithelium into periodic pattern gives rise villi, finger-like protrusions that enable nutrient absorption. However, molecular and mechanical processes driving villus morphogenesis remain unclear. Here, we identify an active mechanism simultaneously patterns intestinal initiate formation. At cellular level, find PDGFRA+ subepithelial mesenchymal cells generate myosin II-dependent forces sufficient produce patterned curvature in neighboring tissue interfaces. This symmetry-breaking process requires altered cell extracellular matrix interactions enabled by metalloproteinase-mediated fluidization. Computational models, together with vitro vivo experiments, revealed these features manifest at level as differences interfacial tensions promote aggregation interface through analogous dewetting thin liquid film.

Language: Английский

Citations

24
Tissue Morphogenesis Through Dynamic Cell and Matrix Interactions DOI Creative Commons
Di Wu, Kenneth M. Yamada, Shaohe Wang

et al.

Annual Review of Cell and Developmental Biology, Journal Year: 2023, Volume and Issue: 39(1), P. 123 - 144

Published: June 14, 2023

Multicellular organisms generate tissues of diverse shapes and functions from cells extracellular matrices. Their adhesion molecules mediate cell-cell cell-matrix interactions, which not only play crucial roles in maintaining tissue integrity but also serve as key regulators morphogenesis. Cells constantly probe their environment to make decisions: They integrate chemical mechanical information the via diffusible ligand- or adhesion-based signaling decide whether release specific enzymes, divide differentiate, move away stay, even live die. These decisions turn modify environment, including nature properties matrix. Tissue morphology is physical manifestation remodeling matrices by historical biochemical biophysical landscapes. We review our understanding matrix morphogenesis, with an emphasis on interactions that drive

Language: Английский

Citations

24
Advancing Synthetic Hydrogels through Nature‐Inspired Materials Chemistry DOI Creative Commons
Bram G. Soliman,

Ashley K. Nguyen,

J. Justin Gooding

et al.

Advanced Materials, Journal Year: 2024, Volume and Issue: unknown

Published: June 19, 2024

Synthetic extracellular matrix (ECM) mimics that can recapitulate the complex biochemical and mechanical nature of native tissues are needed for advanced models development disease. Biomedical research has heavily relied on use animal-derived biomaterials, which is now impeding their translational potential convoluting biological insights gleaned from in vitro tissue models. Natural hydrogels have long served as a convenient effective cell culture tool, but advances materials chemistry fabrication techniques present promising new avenues creating xenogenic-free ECM substitutes appropriate organotypic microphysiological systems. However, significant challenges remain synthetic matrices approximate structural sophistication, complexity, dynamic functionality tissues. This review summarizes key properties ECM, discusses recent approaches used to systematically decouple tune these matrices. The importance mechanics, such viscoelasticity plasticity, also discussed, particularly within context organoid engineered Emerging design strategies mimic reviewed, multi-network hydrogels, supramolecular chemistry, assembled monomers.

Language: Английский

Citations

13
Stimuli-Responsive Polymers at the Interface with Biology DOI
Nathan R. B. Boase, Elizabeth R. Gillies, Rubayn Goh

et al.

Biomacromolecules, Journal Year: 2024, Volume and Issue: 25(9), P. 5417 - 5436

Published: Aug. 28, 2024

There has been growing interest in polymeric systems that break down or undergo property changes response to stimuli. Such polymers can play important roles biological systems, where they be used control the release of therapeutics, modulate imaging signals, actuate movement, direct growth cells. In this Perspective, after discussing most stimuli relevant applications, we will present a selection recent exciting developments. The importance stimuli-responsive polysaccharides discussed, followed by variety for delivery small molecule drugs and nucleic acids. Switchable emerging area therapeutic measurement theranostics described. Then, diverse functions achieved using hydrogels cross-linked covalently, as well various dynamic approaches presented. Finally, discuss some challenges future perspectives field.

Language: Английский

Citations

12
4D printing for biomedical applications DOI
Arkodip Mandal, Kaushik Chatterjee

Journal of Materials Chemistry B, Journal Year: 2024, Volume and Issue: 12(12), P. 2985 - 3005

Published: Jan. 1, 2024

Schematic illustration of 4D printing.

Language: Английский

Citations

11
Stiffness anisotropy coordinates supracellular contractility driving long-range myotube-ECM alignment DOI Creative Commons
Nathaniel P. Skillin, Bruce E. Kirkpatrick, Katie M. Herbert

et al.

Science Advances, Journal Year: 2024, Volume and Issue: 10(22)

Published: May 31, 2024

The ability of cells to organize into tissues with proper structure and function requires the effective coordination proliferation, migration, polarization, differentiation across length scales. Skeletal muscle is innately anisotropic; however, few biomaterials can emulate mechanical anisotropy determine its influence on tissue patterning without introducing confounding topography. Here, we demonstrate that substrate stiffness coordinates contractility-driven collective cellular dynamics resulting in C2C12 myotube alignment over millimeter-scale distances. When cultured mechanically anisotropic liquid crystalline polymer networks (LCNs) lacking topography, myoblasts collectively polarize stiffest direction. Cellular amplified through reciprocal cell-ECM emerge during fusion, driving global myotube-ECM ordering. Conversely, was restricted small local domains no directional preference isotropic LCNs same chemical formulation. These findings provide valuable insights for designing mimic microenvironments underscore importance orchestrating morphogenesis.

Language: Английский

Citations

10
Probing organoid metabolism using fluorescence lifetime imaging microscopy (FLIM): The next frontier of drug discovery and disease understanding DOI Creative Commons
Margarida Barroso, Michael G. Monaghan, Raluca Niesner

et al.

Advanced Drug Delivery Reviews, Journal Year: 2023, Volume and Issue: 201, P. 115081 - 115081

Published: Aug. 28, 2023

Language: Английский

Citations

18
Radical‐Mediated Degradation of Thiol–Maleimide Hydrogels DOI
Tayler S. Hebner, Bruce E. Kirkpatrick,

Benjamin D. Fairbanks

et al.

Advanced Science, Journal Year: 2024, Volume and Issue: 11(25)

Published: April 6, 2024

Michael addition between thiol- and maleimide-functionalized molecules is a long-standing approach used for bioconjugation, hydrogel crosslinking, the functionalization of other advanced materials. While simplicity this chemistry enables facile synthesis hydrogels, network degradation also desirable in many instances. Here, susceptibility thiol-maleimide bonds to radical-mediated reported. Irreversible crosslinked materials demonstrated using photoinitiated chemically initiated radicals hydrogels linear polymers. The extent shown be dependent on initiator concentration. Using model polymer system, mechanism elucidated, which thiosuccinimide crosslink converted succinimide new thioether formed with an fragment. laser stereolithography, high-fidelity spatiotemporal control over gels demonstrated. Ultimately, work establishes platform controllable, further expanding their versatility as functional

Language: Английский

Citations

6
Orally-administered nanomedicine systems targeting colon inflammation for the treatment of inflammatory bowel disease: latest advances DOI
Shumeng Hu, Runan Zhao, Yu Xu

et al.

Journal of Materials Chemistry B, Journal Year: 2023, Volume and Issue: 12(1), P. 13 - 38

Published: Nov. 29, 2023

Inflammatory bowel disease (IBD) is a chronic and idiopathic condition that results in inflammation of the gastrointestinal tract, leading to conditions such as ulcerative colitis Crohn's disease.

Language: Английский

Citations

14