An Easy-to-Use Arrayed Brain–Heart Chip DOI Creative Commons
X.B. Peng, Lei Wu, Qiushi Li

et al.

Biosensors, Journal Year: 2024, Volume and Issue: 14(11), P. 517 - 517

Published: Oct. 22, 2024

Multi-organ chips are effective at emulating human tissue and organ functions replicating the interactions among tissues organs. An arrayed brain-heart chip was introduced whose configuration comprises open culture chambers closed biomimetic vascular channels distributed in a horizontal pattern, separated from each other by an endothelial barrier based on fibrin matrix. A 300 μm-high 13.2 mm-long surrounded organoid chamber, thereby satisfying material transport requirements. Numerical simulations were used to analyze construction process of barriers order optimize structural design experimental manipulation, which exhibited high degree correlation with experiment results. In interconnective unit, cerebral organoid, cardiac cells co-cultured stably for minimum one week. The permeability recirculating perfusion enabled cross talk between organoids organoids, as well cells. This corroborated presence troponin I (cTnI) chamber observation invading matrix after week co-culture. simple manipulate, clearly visible under microscope, compatible automated pipetting devices, therefore had significant potential application.

Language: Английский

Establishment and evaluation of on-chip intestinal barrier biosystems based on microfluidic techniques DOI Creative Commons
Hui Wang, Xiangyang Li, Pengcheng Shi

et al.

Materials Today Bio, Journal Year: 2024, Volume and Issue: 26, P. 101079 - 101079

Published: May 5, 2024

As a booming engineering technology, the microfluidic chip has been widely applied for replicating complexity of human intestinal micro-physiological ecosystems in vitro. Biosensors, 3D imaging, and multi-omics have to engineer more sophisticated barrier-on-chip platforms, allowing improved monitoring physiological processes enhancing performance. In this review, we report cutting-edge advances techniques establishment evaluation barrier platforms. We discuss different design principles microfabrication strategies gut models Further, comprehensively cover complex cell types (e.g., epithelium, organoids, endothelium, microbes, immune cells) controllable extracellular microenvironment parameters oxygen gradient, peristalsis, bioflow, gut-organ axis) used recapitulate main structural functional barriers. also present current multidisciplinary technologies indicators evaluating morphological structure integrity established Finally, highlight challenges future perspectives accelerating broader applications these platforms disease simulation, drug development, personalized medicine. Hence, review provides comprehensive guide development microfluidic-based

Language: Английский

Citations

6
GelMA as scaffold material for epithelial cells to emulate the small intestinal microenvironment DOI Creative Commons
Inez Roegiers, Tom Gheysens, Manon Minsart

et al.

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: March 10, 2025

Host-microbe interactions in the intestine play a significant role health and disease. Novel scaffolds for host cells, capable of potentially supporting these intricate interactions, are necessary to improve our current systems mimicking host-microbiota interplay vitro/ex vivo. In this research paper, we study application gelatin methacrylamide (GelMA) as scaffold material intestinal epithelial cells terms permeability, mechanical strength, biocompatibility. We investigated whether degree substitution (DS) GelMA influences permeability found that both high low DS show sufficient biorelevant transport markers. Additionally, researched cell adherence viability, well characteristics different concentrations GelMA. All hydrogel long-term biocompatibility mono- co-cultures, despite goblet-like (LS174T) showing lower performance than enterocyte-like (Caco-2). The strength all was physiologically relevant range be used cells. Lastly, examined effect two sterilization methods, ethylene oxide (EO) 70% ethanol followed by UVC (EtOH/UVC). impact methods on minimal, did not find between viability confluency Caco-2 seeded hydrogels. Based results, conclude is suitable types These findings contribute growing field vitro modeling gut moves further ensuring more translatable host-microbe interactions.

Language: Английский

Citations

0
Gut‐on‐a‐chip platforms: Bridging in vitro and in vivo models for advanced gastrointestinal research DOI Creative Commons
Awurama Ofori‐Kwafo,

Indira Sigdel,

Earshed Al Mamun

et al.

Physiological Reports, Journal Year: 2025, Volume and Issue: 13(9)

Published: May 1, 2025

Abstract The gastrointestinal (GI) tract plays a critical role in nutrient absorption, immune responses, and overall health. Traditional models such as two‐dimensional cell cultures have provided valuable insights but fail to replicate the dynamic complex microenvironment of human gut. Gut‐on‐a‐chip platforms, which incorporate cells located gut into microfluidic devices that simulate peristaltic motion fluid flow, represent significant advancement modeling GI physiology diseases. This review discusses evolution gut‐on‐a‐chip technology, from simple cellular mono‐cultures more sophisticated systems incorporating bi‐cultures tri‐cultures enable studies drug metabolism, disease modeling, gut–microbiome interactions. Although challenges remain, including maintaining long‐term viability replicating these platforms hold great potential for advancing personalized medicine improving discovery efforts targeting disorders.

Language: Английский

Citations

0
An in vitro multi-organ microphysiological system (MPS) to investigate the gut-to-brain translocation of neurotoxins DOI
Emily Jones, Benjamin M. Skinner, Aimée Parker

et al.

Biomicrofluidics, Journal Year: 2024, Volume and Issue: 18(5)

Published: Sept. 1, 2024

The death of dopamine-producing neurons in the substantia nigra base brain is a defining pathological feature development Parkinson's disease (PD). PD is, however, multi-systemic disease, also affecting peripheral nervous system and gastrointestinal tract (GIT) that interact via gut-brain axis (GBA). Our dual-flow GIT-brain microphysiological (MPS) was modified to investigate gut-to-brain translocation neurotoxin trigger PD, 1-methyl-4-phenylpyridinium (MPP

Language: Английский

Citations

1
An Easy-to-Use Arrayed Brain–Heart Chip DOI Creative Commons
X.B. Peng, Lei Wu, Qiushi Li

et al.

Biosensors, Journal Year: 2024, Volume and Issue: 14(11), P. 517 - 517

Published: Oct. 22, 2024

Multi-organ chips are effective at emulating human tissue and organ functions replicating the interactions among tissues organs. An arrayed brain-heart chip was introduced whose configuration comprises open culture chambers closed biomimetic vascular channels distributed in a horizontal pattern, separated from each other by an endothelial barrier based on fibrin matrix. A 300 μm-high 13.2 mm-long surrounded organoid chamber, thereby satisfying material transport requirements. Numerical simulations were used to analyze construction process of barriers order optimize structural design experimental manipulation, which exhibited high degree correlation with experiment results. In interconnective unit, cerebral organoid, cardiac cells co-cultured stably for minimum one week. The permeability recirculating perfusion enabled cross talk between organoids organoids, as well cells. This corroborated presence troponin I (cTnI) chamber observation invading matrix after week co-culture. simple manipulate, clearly visible under microscope, compatible automated pipetting devices, therefore had significant potential application.

Language: Английский

Citations

0