Liver organoids: From 3D printing to biomedical applications DOI
Ying Shi,

Xin Han,

Zheng Zhang

et al.

BMEMat, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 8, 2024

Abstract The liver is an immune organ, especially tolerance organ. critical shortage of donor organs and disease models for the treatment end‐stage failure underscores urgent need generation organoids from human induced pluripotent stem cells (iPSCs). Notably, significant advancements have been made in study over past decade. construction has transitioned single cell type to multicellular models, two‐dimensional three‐dimensional cultures. Here we provide progress surrounding different culture techniques 3D printing organ‐on‐chip, as well focuses on present future applications organoids, then challenges perspectives ahead further advancement.

Language: Английский

Microfluidic organ-on-a-chip models for the gut–liver axis: from structural mimicry to functional insights DOI
Weifeng Hu, Yushen Wang,

Junlei Han

et al.

Biomaterials Science, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

This review discusses advancements in organ-on-a-chip models, emphasizing gut, liver, and integrated gut–liver systems. It covers their applications disease modeling, toxicity testing, drug screening, as well future prospects.

Language: Английский

Citations

0
A new dawn: Vitalising translational oncology research in Africa with the help of advanced cell culture models DOI
Stefanie Klima, Tracey Hurrell, Mubeen Goolam

et al.

Translational Oncology, Journal Year: 2025, Volume and Issue: 56, P. 102391 - 102391

Published: April 14, 2025

Language: Английский

Citations

0
Assessing immune hepatotoxicity of troglitazone with a versatile liver-immune-microphysiological-system DOI Creative Commons
Quanfeng Deng, Youlong Yang, Yuangui Liu

et al.

Frontiers in Pharmacology, Journal Year: 2024, Volume and Issue: 15

Published: May 30, 2024

Drug-induced liver injury is a prevalent adverse event associated with pharmaceutical agents. More significantly, there are certain drugs that present severe hepatotoxicity only during the clinical phase, consequently leading to termination of drug development trials or withdrawal from market after approval. The establishment an evaluation model can sensitively manifest such has always been challenging aspect in development. In this study, we build liver-immune-microphysiological-system (LIMPS) fully demonstrate triggered by troglitazone (TGZ), was withdrawn due hepatotoxicity. Leveraging capabilities organ-on-chip technology allows for dynamic modulation cellular immune milieu, as well synergistic effects between drugs, hepatocytes and multiple cells. Through LIMPS, discovered 1) TGZ promote neutrophils adhered hepatocytes, 2) presence enhances crosstalk macrophages neutrophils, 3) induction damage at clinically relevant blood concentrations not observed other

Language: Английский

Citations

2
Generation of in vivo-like multicellular liver organoids by mimicking developmental processes: A review DOI Creative Commons
Ayumu Okumura, Kenji Aoshima, Naoki Tanimizu

et al.

Regenerative Therapy, Journal Year: 2024, Volume and Issue: 26, P. 219 - 234

Published: June 1, 2024

Liver is involved in metabolic reactions, ammonia detoxification, and immunity. Multicellular liver tissue cultures are more desirable for drug screening, disease modeling, researching transplantation therapy, than hepatocytes monocultures. Hepatocytes monocultures not stable long. Further, hepatocyte-like cells induced from pluripotent stem vivo functionally dissimilar. Organoid technology circumvents these issues by generating functional ex intrinsic progenitor extrinsic cells, including cells. To function as tissue, the organoid must be arranged precisely 3-dimensional space, closely mimicking tissue. Moreover, long term functioning, organoids appropriately vascularized contact with neighboring epithelial tissues (e.g., bile canaliculi intrahepatic duct, or extrahepatic ducts). Recent discoveries developmental biology allows one to successfully induce component generate organoids. Thus, here, this review, we summarize current state of knowledge on development a focus its application different We also cover future prospects creating (functionally structurally) vivo-like using development.

Language: Английский

Citations

2
Organ-specific vasculature-on-a-chip systems DOI Open Access
Hasan Erbil Abaci, Mandy B. Esch

Biomicrofluidics, Journal Year: 2024, Volume and Issue: 18(3)

Published: May 1, 2024

Language: Английский

Citations

1
Liver organoids: From 3D printing to biomedical applications DOI
Ying Shi,

Xin Han,

Zheng Zhang

et al.

BMEMat, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 8, 2024

Abstract The liver is an immune organ, especially tolerance organ. critical shortage of donor organs and disease models for the treatment end‐stage failure underscores urgent need generation organoids from human induced pluripotent stem cells (iPSCs). Notably, significant advancements have been made in study over past decade. construction has transitioned single cell type to multicellular models, two‐dimensional three‐dimensional cultures. Here we provide progress surrounding different culture techniques 3D printing organ‐on‐chip, as well focuses on present future applications organoids, then challenges perspectives ahead further advancement.

Language: Английский

Citations

0