Cited by Platelet Proteomics: Clinical Implications

Neuroprotective effects of intranasal extracellular vesicles from human platelet concentrates supernatants in traumatic brain injury and Parkinson’s disease models DOI

Liling Delila, Ouada Nebie, Nhi Thao Ngoc Le

et al.

Journal of Biomedical Science, Journal Year: 2024, Volume and Issue: 31(1)

Published: Sept. 5, 2024

The burgeoning field of regenerative medicine has significantly advanced with recent findings on biotherapies using human platelet lysates (HPLs), derived from clinical-grade concentrates (PCs), for treating brain disorders. These developments have opened new translational research avenues to explore the neuroprotective effects platelet-extracellular vesicles (PEVs). Their potential in managing neurodegenerative conditions like traumatic injury (TBI) and Parkinson's disease (PD) warrants further exploration. We aimed here characterize composition a PEV preparation isolated concentrate (PC) supernatant, determine its neurorestorative cellular animal models TBI PD.

Language: Английский

Citations

Human platelet lysate: a potential therapeutic for intracerebral hemorrhage DOI

Dachang Qiu,

Lin Wang, Lanlan Wang

et al.

Frontiers in Neuroscience, Journal Year: 2025, Volume and Issue: 18

Published: Jan. 15, 2025

Intracerebral hemorrhage (ICH) is a major public health challenge worldwide, and associated with elevated rates of mortality, disability, morbidity, especially in low- middle-income nations. However, our knowledge the detailed molecular processes involved ICH remains insufficient, particularly those secondary injury stage, resulting lack effective treatments for ICH. Human platelet lysates (HPL) are abundant bioactive factors, numerous studies have demonstrated their beneficial effects on neurological diseases, including anti-neuroinflammatory ability, anti-oxidant effects, maintenance blood-brain barrier integrity, promotion neurogenesis. In this review, we thoroughly explore potential HPL treating from three critical perspectives: rationale selecting as treatment ICH, mechanisms through which contributes to management, additional measures necessary We elucidate role platelets pathophysiology highlight limitations current options advancements preclinical research application disorders. Furthermore, historical developments preparation methods field biomedicine discussed. Additionally, summarize molecules present therapeutic Finally, outline issues that must be addressed regarding utilizing modality

Language: Английский

Citations

Intranasal delivery of epigallocatechin gallate-laden platelet extracellular vesicles for mitigating retinal glaucoma DOI

Wen-Yu Pan,

Pei-Wei Weng,

Shen-Han Wu

et al.

Journal of Controlled Release, Journal Year: 2025, Volume and Issue: unknown, P. 113596 - 113596

Published: March 1, 2025

Language: Английский

Citations

Bioprocessing of human platelet concentrates to generate lysates and extracellular vesicles for therapeutic applications DOI

Wei-Ting Yeh,

Ezrin Yi-Ling Yu,

Ya-Hsuan Lu

et al.

MethodsX, Journal Year: 2024, Volume and Issue: 13, P. 102822 - 102822

Published: July 9, 2024

This work describes protocols for preparing specific forms of human platelet lysates from pooled concentrates (PCs) and the isolation platelet-derived extracellular vesicles (p-EVs). Clinical-grade PCs can be sourced blood establishments immediately following expiration transfusion use. Here, we describe methods to process into which p-EVs isolated. Each lysate type is prepared using activation processing produce distinct products that may useful in different applications. For example, serum-converted (SCPL)-EVs were recently shown have powerful therapeutic properties myocardial infarction mice. EVs isolated all size exclusion chromatography, producing pure consistent multiple batches. Together, these allow with excellent potential clinical preclinical applications.•Platelet obtained local are reliable sustainable sources generate biomaterials.•We outline five generation one method vesicle isolation.•Each form has biological suitable certain

Language: Английский

Citations

Pilot study characterizing a single pooled preparation of equine platelet lysate for nebulization in the horse DOI

Patricia Egli,

Lindsey Boone, Laura Huber

et al.

Frontiers in Veterinary Science, Journal Year: 2024, Volume and Issue: 11

Published: Dec. 12, 2024

Introduction Platelet lysate (PL) demonstrates antimicrobial and anti-inflammatory properties offering potential for treatment of bacterial pneumonia in horses. It remains unknown whether nebulization is suitable PL administration This pilot study characterized particle size flow rate pooled equine (single preparation) nebulized using an equine-specific nebulizer (Flexivent ® ). Methods Protein composition activity were compared before after nebulization. was evaluated according to growth factor, peptide cytokine concentrations proteomic analysis. To evaluate activity, inhibition [maximum (μmax); carrying capacity (K)] determined E. coli, Streptococcus equi subsp zooepidemicus Rhodococcus (WT MDR) pre- post-nebulized 50%. Results Flow median 0.8 ml/min 4.991 μm with 52% particles ≤ 5 μm. Differences protein detected For coli S. , did not alter effect on parameters. treatments decreased K ( p = 0.009) BHI. R. increased post- vs. pre-nebulization μmax vs, post-nebulization (MDR). MDR WT BHI 0.05). Conclusion Nebulization vitro technically feasible. The results this support further investigation better characterize the its suitability

Language: Английский

Citations

Platelet Proteomics: Clinical Implications – Decoding the Black Box! DOI

Hadi Goubran, Shahid Ahmed, Gaafar Ragab

et al.

Transfusion and Apheresis Science, Journal Year: 2024, Volume and Issue: 64(1), P. 104060 - 104060

Published: Dec. 17, 2024

Language: Английский

Citations