Animals,
Journal Year:
2020,
Volume and Issue:
10(11), P. 1993 - 1993
Published: Oct. 29, 2020
In
order
to
optimize
the
donkey
sperm
motility
analysis
by
CASA
(Computer
Assisted
Sperm
Analysis)-Mot
system,
twelve
ejaculates
were
collected
from
six
jackasses.
Capillary
loaded
chamber
(CLC),
ISAS®D4C
depths
10
and
20
µm,
Leja
drop
displacement
(DDC),
Spermtrack®
(Spk)
µm
used.
kinematic
variables
evaluated
using
each
a
high-resolution
camera
capable
of
capturing
maximum
500
frames/second
(fps).
The
optimum
frame
rate
(OFR)
(defined
according
curvilinear
velocity-VCL)
was
dependent
on
type.
highest
OFR
obtained
278.46
fps
Spk20.
Values
for
VCL,
straight-line
velocity
(VSL),
straightness
(STR),
amplitude
lateral
head
(ALH)
beat
cross
frequency
(BCF)
high
in
DDC
depth.
both
velocities
(VCL,
VSL,
VAP)
ALH
values
decreased
significantly
centre
edges,
while
Wobble
BCF
increased.
No
defined
behavior
observed
along
CLC.
However,
all
had
higher
value
highly
concentrated
sample,
types.
conclusion,
analyzing
minimum
nine
fields
at
250
edges
Spk10
dilution
30
×
106
sperm/mL
offers
best
choice
computerised
analysis.
Animal Reproduction Science,
Journal Year:
2021,
Volume and Issue:
246, P. 106904 - 106904
Published: Dec. 3, 2021
Sperm
cryopreservation
is
one
of
the
most
important
procedures
in
development
biotechnologies
for
assisted
reproduction.
In
some
farm
animals,
use
cryopreserved
sperm
has
so
many
benefits
which
relevance
become
more
evident
recent
decades.
Values
post-thaw
quality,
however,
are
variable
among
species
and
within
individuals
same
species.
There
no
standardized
methodology
each
stages
procedure
(andrological
examination,
semen
collection,
dilution,
centrifugation,
resuspension
pellet
with
freezing
medium,
packaging,
evaluation),
also
contributes
to
differences
studies.
Cryotolerance
markers
seminal
plasma
(SP)
have
been
evaluated
prediction
ejaculate
freezability.
addition,
previous
research,
there
a
focus
on
supplementing
media
different
substances,
such
as
enzymatic
non-enzymatic
antioxidants.
studies,
inclusion
these
substances
led
improved
quality
fertilizing
capacity
result
minimizing
adverse
effects
structure
function.
Another
approach
cryoprotectants.
The
aim
this
review
article
provide
an
update
animals.
main
detrimental
described,
including
negative
repercussion
reproductive
performance.
Furthermore,
potential
molecular
biomarkers
predict
cryotolerance
discussed,
well
addition
that
can
mitigate
harmful
impact
thawing
sperm.
Reproduction Fertility and Development,
Journal Year:
2018,
Volume and Issue:
30(6), P. 810 - 810
Published: Jan. 1, 2018
For
over
30
years,
CASA-Mot
technology
has
been
used
for
kinematic
analysis
of
sperm
motility
in
different
mammalian
species,
but
insufficient
attention
paid
to
the
technical
limitations
commercial
computer-aided
(CASA)
systems.
Counting
chamber
type
and
frame
rate
are
two
most
important
aspects
be
taken
into
account.
chambers
can
disposable
or
reusable,
with
depths.
In
human
semen
analysis,
reusable
a
depth
10µm
frequently
used,
whereas
farm
animal
species
it
is
more
common
use
20µm
.
The
was
previously
limited
by
hardware,
although
changes
number
images
collected
could
lead
significant
variations
some
parameters,
mainly
curvilinear
velocity
(VCL).
A
60
frames
s-1
widely
considered
minimum
necessary
satisfactory
results.
However,
specific
must
defined
each
experimental
condition.
conclusion,
we
show
that
optimal
combination
counting
should
condition
order
obtain
reliable
Biology,
Journal Year:
2022,
Volume and Issue:
11(12), P. 1715 - 1715
Published: Nov. 26, 2022
For
successful
fertilisation
to
occur,
spermatozoa
need
successfully
migrate
through
the
female
reproductive
tract
and
penetrate
oocyte.
Predictably,
poor
sperm
motility
has
been
associated
with
low
rates
of
in
many
mammalian
species,
including
ram.
As
such,
is
one
most
important
parameters
used
for
vitro
evaluation
ram
quality
function.
This
review
aims
outline
mechanical
energetic
processes
which
underpin
motility,
describe
changes
occur
as
a
result
differences
structure
surrounding
microenvironment,
assess
effectiveness
various
methods
rams.
Methods
subjective
estimation
are
convenient,
inexpensive
widely
livestock
industries,
however,
nature
these
can
make
them
unreliable.
Computer-assisted
analysis
(CASA)
technology
accurately
objectively
measures
via
two-dimensional
tracing
head
motion,
making
it
popular
method
assurance
domesticated
animal
production
laboratories.
Newly
developed
assessment
flagellar
tracing,
three-dimensional
vivo
assessment,
molecular
assays
quantify
motility-associated
biomarkers,
enable
new
range
motion
potential
reveal
mechanistic
insights
improve
semen
assessment.
Experimental
application
technologies
required
fully
understand
their
prediction
success
ovine
artificial
breeding
programs.
Reproduction Fertility and Development,
Journal Year:
2018,
Volume and Issue:
30(6), P. 875 - 875
Published: Jan. 1, 2018
Since
the
advent
of
computer-aided
sperm
analysis
(CASA)
some
four
decades
ago,
advances
in
computer
technology
and
software
algorithms
have
helped
establish
it
as
a
research
diagnostic
instrument
for
spermatozoa.
Despite
mammalian
spermatozoa
being
most
diverse
cell
type
known,
CASA
is
great
tool
that
has
capacity
to
provide
rapid,
reliable
objective
quantitative
assessment
quality.
This
paper
provides
contemporary
findings
illustrating
scientific
commercial
applications
its
ability
evaluate
(human,
primates,
rodents,
domestic
mammals,
wildlife
species)
at
both
structural
functional
levels.
The
potential
quantitatively
measure
essential
aspects
related
subpopulations,
hyperactivation,
morphology
morphometry
also
demonstrated.
Furthermore,
are
provided
improved
quality
assessment,
evaluation
functionality
effect
different
chemical
substances
or
pathologies
on
fertilising
ability.
It
clear
evolved
significantly
currently
superior
many
manual
techniques
clinical
setting.
Reproduction in Domestic Animals,
Journal Year:
2018,
Volume and Issue:
54(2), P. 167 - 175
Published: Sept. 1, 2018
Contents
Motility
is
the
most
widely
used
indicator
of
sperm
quality.
Computer‐Assisted
Semen
Analysis
(
CASA
)
allows
objective
evaluation
motility
parameters.
technology
a
common
tool
to
predict
semen
doses
in
farm
animal
reproduction.
The
kinds
video
cameras
until
now
for
image
acquisition
have
presented
limited
frame
rates
FR
),
which
negative
influence
on
quality
obtained
data.
aim
present
work
was
define
optimal
rate
correct
boar
and
its
subpopulation
structure.
Eighteen
ejaculates
from
nine
mature
boars
Pietrain
breed
were
used.
Using
ISAS
®
v1
‐Mot
system,
with
camera
working
up
200
Hz,
six
s
(25,
50,
75,
100,
150
fps)
compared.
D4C20
counting
chambers,
warmed
37°C,
affected
all
kinematic
parameters,
curvilinear
velocity
VCL
BCF
sensitive
ones.
All
parameters
showed
differences
among
animals.
Non‐linear
correlation
asymptotic
level
at
212
fps,
being
highest
For
future
studies
based
just
progressive
motility,
almost
100
fps
0.5
must
be
used,
while
when
kinematics
considered,
one‐second
should
analysed.
Three
principal
components
(velocity,
progressivity
oscillation),
similar
50
fps.
Cells
grouped
four
subpopulations
but
different
cellular
distribution
both
s.
Animals,
Journal Year:
2025,
Volume and Issue:
15(3), P. 305 - 305
Published: Jan. 22, 2025
Spermatozoa
are
highly
specialized
male
cells
that
characterized
by
a
unique
ability
to
move,
which
is
critical
factor
for
successful
fertilization.
The
relative
simplicity
of
motility
assessment,
especially
in
livestock,
has
made
it
widely
used
parameter
evaluating
ejaculate
quality
or
cryopreserved
semen
the
clinical
field,
and
an
advanced
tool
reproductive
physiology
toxicology
research.
Technological
advances
image
analysis
computational
methods
have
substantially
increased
its
accuracy
through
use
computer-assisted
sperm
(CASA)
minimize
subjective
bias
assessments.
Nevertheless,
this
more
objective
method
still
presents
some
significant
challenges,
including
variability
sample
preparation,
imaging
conditions,
analytical
parameters.
These
issues
contribute
inconsistency
impair
reproducibility
comparability
data
between
laboratories.
implementation
standardized
protocols,
combined
with
comprehensive
training
rigorous
evaluation,
can
serve
mitigate
emerging
inconsistencies.
In
addition,
vitro
conditions
under
CASA
analyses
performed
often
differ
significantly
from
natural
environment
female
tract
vivo.
This
review
discusses
methodologies,
issues,
limitations
using
CASA,
particular
focus
on
boar
as
important
agricultural
biomedical
model
species
system
used.
Biology,
Journal Year:
2020,
Volume and Issue:
9(6), P. 138 - 138
Published: June 26, 2020
The
ejaculate
is
heterogenous
and
sperm
sub-populations
with
different
kinematic
patterns
can
be
identified
in
various
species.
Nevertheless,
although
these
are
statistically
well
defined,
the
statistical
differences
not
always
relevant.
aim
of
present
study
was
to
characterize
from
two
bovine
species,
diluted
commercial
extenders,
determine
relevance
through
Bayesian
analysis.
Semen
10
bulls
evaluated
after
thawing.
An
ISAS®v1
computer-assisted
analysis
(CASA)-Mot
system
employed
an
image
acquisition
rate
50
Hz
ISAS®D4C20
counting
chambers.
Sub-populations
motile
spermatozoa
were
characterized
using
multivariate
procedures
such
as
principal
components
(PCs)
clustering
methods
(k-means
model).
Four
three
PCs
that
involved
progressiveness,
velocity,
cell
undulatory
movement.
proportions
varied
extender
used
Despite
a
difference
(p
<
0.05)
between
confirmed
only
one
them
(Triladyl®)
presented
relevant
when
compared
Tris-EY
OptiXcell®.
Extenders
differed
proportion
cells
each
sub-populations.
Similar
Bos
taurus
indicus.
results
indicate
SP1,
SP2,
SP3
for
PC
criteria
For
linearity,
SP4
did
show
regarding
other
classical
approach
or
subpopulation
thus
may
have
direct
biological
meaning.
Therefore,
needs
reevaluated.
