El análisis seminal en la agricultura de precisión en el siglo XXI DOI Creative Commons
Carles Soler, Anthony Valverde

Agronomía Mesoamericana, Journal Year: 2023, Volume and Issue: unknown, P. 51957 - 51957

Published: Jan. 30, 2023

Introducción. La reproducción asistida de animales tiene su origen en la domesticación las especies ganaderas útiles al ser humano. Los consiguientes procesos selección artificial permitieron desarrollar técnicas biotecnológicas que contribuyeron a mejora capacidades producción animal. Objetivo. Contextualizar el análisis seminal agricultura precisión siglo XXI. Desarrollo. visualización espermatozoides microscopio puede considerarse como primer paso advenimiento biotecnología y base para desarrollo asistida. Con perfeccionamiento los métodos microscopía, se logró caracterizar gametos masculinos, lo significó un avance significativo tecnología inseminación artificial. El punto inflexión marcado por conservación espermática, implicó cambio sustantivo estas tecnologías ganaderas, ya sea con semen crio preservado o refrigerado. Estos son alto valor caso amenazadas, dado pueden crear bancos germoplasma propósitos genética rescate riesgo extinción. ha desarrollado forma paralela asistida, hoy considera una técnica relevante animal, perfeccionado mediante ciencia tecnología, física óptica computación. Conclusión. tenido paradigma desechar obsoletas evaluación subjetiva calidad adoptar objetivos del semen, grandes volúmenes datos variables movilidad, cinéticas, morfométricas, morfológicas fragmentación ADN, permiten mejor eyaculados reproductores centros

Sperm bauplan and function and underlying processes of sperm formation and selection DOI Creative Commons
María E. Teves, Eduardo R. S. Roldán

Physiological Reviews, Journal Year: 2021, Volume and Issue: 102(1), P. 7 - 60

Published: April 21, 2021

The spermatozoon is a highly differentiated and polarized cell, with two main structures: the head, containing haploid nucleus acrosomal exocytotic granule, flagellum, which generates energy propels cell; both structures are connected by neck. sperm’s aim to participate in fertilization, thus activating development. Despite this common bauplan function, there an enormous diversity structure performance of sperm cells. For example, mammalian spermatozoa may exhibit several head patterns overall lengths ranging from ∼30 350 µm. Mechanisms transport female tract, preparation for recognition interaction oocyte also show considerable variation. There has been much interest understanding origin diversity, evolutionary terms relation mechanisms underlying differentiation testis. Here, relationships between function examined at levels: first, analyzing selective forces that drive changes physiology understand adaptive values variation impact on male reproductive success second, examining cellular molecular formation testis explain how can give rise such wide array forms functions.

Language: Английский

Citations

57

Motile sperm subpopulations in bull semen using different clustering approaches – Associations with flow cytometric sperm characteristics and fertility DOI
Iulian Ibănescu,

Mathias Siuda,

H. Bollwein

et al.

Animal Reproduction Science, Journal Year: 2020, Volume and Issue: 215, P. 106329 - 106329

Published: Feb. 21, 2020

Language: Английский

Citations

49

Antioxidant Effects of Myo-Inositol Improve the Function and Fertility of Cryopreserved Boar Semen DOI Creative Commons
Rana Osman, Seongju Lee, Areeg Almubarak

et al.

Antioxidants, Journal Year: 2023, Volume and Issue: 12(9), P. 1673 - 1673

Published: Aug. 26, 2023

During cryopreservation, sperm undergoes structural and molecular changes such as ice crystal formation, DNA fragmentation, reactive oxygen species (ROS) production, leading to decreased quality after thawing. Antioxidants play a crucial role in preventing these damages, both vivo vitro. One potent antioxidant is myo-inositol, known for its protective effects on against ROS. This study aimed investigate the effect of myo-inositol cryopreserved boar semen. The semen was diluted, cooled, using BF5 extender. It then divided into five groups: control different concentrations (0.5, 1, 1.5, 2 mg/mL). post-thaw evaluation included assessments motility, viability, acrosome integrity, mitochondrial membrane potential (MMP), caspase activity, gene expression, ROS levels, apoptosis, IVF with treated Results showed that at 0.5 mg/mL improved fertilization ability. also reduced expression pro-apoptotic genes increased SMCP expression. Lower demonstrated viability apoptosis levels. In conclusion, treatment during cryopreservation quality, enhanced fertility rates

Language: Английский

Citations

13

Computer Aided Sperm Analysis (CASA) in domestic animals: Current status, three D tracking and flagellar analysis DOI
Gerhard van der Horst

Animal Reproduction Science, Journal Year: 2020, Volume and Issue: 220, P. 106350 - 106350

Published: April 4, 2020

Language: Английский

Citations

35

Kinematic Sub-Populations in Bull Spermatozoa: A Comparison of Classical and Bayesian Approaches DOI Creative Commons
Luis Víquez, Vinicio Barquero, Carles Soler

et al.

Biology, Journal Year: 2020, Volume and Issue: 9(6), P. 138 - 138

Published: June 26, 2020

The ejaculate is heterogenous and sperm sub-populations with different kinematic patterns can be identified in various species. Nevertheless, although these are statistically well defined, the statistical differences not always relevant. aim of present study was to characterize from two bovine species, diluted commercial extenders, determine relevance through Bayesian analysis. Semen 10 bulls evaluated after thawing. An ISAS®v1 computer-assisted analysis (CASA)-Mot system employed an image acquisition rate 50 Hz ISAS®D4C20 counting chambers. Sub-populations motile spermatozoa were characterized using multivariate procedures such as principal components (PCs) clustering methods (k-means model). Four three PCs that involved progressiveness, velocity, cell undulatory movement. proportions varied extender used Despite a difference (p < 0.05) between confirmed only one them (Triladyl®) presented relevant when compared Tris-EY OptiXcell®. Extenders differed proportion cells each sub-populations. Similar Bos taurus indicus. results indicate SP1, SP2, SP3 for PC criteria For linearity, SP4 did show regarding other classical approach or subpopulation thus may have direct biological meaning. Therefore, needs reevaluated.

Language: Английский

Citations

32

Predictive Capacity of Boar Sperm Morphometry and Morphometric Sub-Populations on Reproductive Success after Artificial Insemination DOI Creative Commons
Vinicio Barquero, Eduardo R. S. Roldán, Carles Soler

et al.

Animals, Journal Year: 2021, Volume and Issue: 11(4), P. 920 - 920

Published: March 24, 2021

The aim of the study was to compare morphometric features sperm head size and shape from Pietrain line Duroc × boar crossbred terminal lines, evaluate their relationship with reproductive success after artificial insemination sows produced crossbreeding York, Landrace breeds. Semen samples were collected 11 sexually mature boars. Only ejaculates greater than 70% motility rate <15% abnormal used for inseminations (AI) included in study. Samples analyzed using an ISAS®v1 computer-assisted analysis system eight parameters (CASA-Morph). Sub-populations characterized multivariate procedures, such as principal component (PC) clustering methods (k-means model). Four different ejaculate sub-populations identified two PCs that involved spermatozoa. discriminant ability variables predict sow litter a receiver operating characteristics (ROC) curve analysis. Sperm length, ellipticity, elongation, regularity showed significant predictive capacity on (0.59, 0.59, 0.60, 0.56 area under (AUC), respectively). not related size.

Language: Английский

Citations

24

Implications of boar sperm kinematics and rheotaxis for fertility after preservation DOI
S. Fair, Jon Romero‐Aguirregomezcorta

Theriogenology, Journal Year: 2019, Volume and Issue: 137, P. 15 - 22

Published: May 31, 2019

Language: Английский

Citations

27

Standardization of sperm motility analysis by using CASA-Mot for Atlantic salmon (Salmo salar), European eel (Anguilla anguilla) and Siberian sturgeon (Acipenser baerii) DOI

Carina Caldeira,

Sandra Hernández-Ibáñez, Anthony Valverde

et al.

Aquaculture, Journal Year: 2018, Volume and Issue: 502, P. 223 - 231

Published: Dec. 3, 2018

Language: Английский

Citations

27

Combined effects of type and depth of counting chamber, and rate of image frame capture, on bull sperm motility and kinematics DOI
Daznia Bompart,

Rubén F. Vázquez,

Rubén Sánchez Gómez

et al.

Animal Reproduction Science, Journal Year: 2019, Volume and Issue: 209, P. 106169 - 106169

Published: Aug. 20, 2019

Language: Английский

Citations

23

The application of computer-assisted semen analysis (CASA) technology to optimise semen evaluation. A review DOI Open Access
Anthony Valverde, Vinicio Barquero, Carles Soler

et al.

Journal of Animal and Feed Sciences, Journal Year: 2020, Volume and Issue: 29(3), P. 189 - 198

Published: Sept. 21, 2020

1. Acosta A.A., Kruger T.F. (Editors), 1996. Human Spermatozoa in Assisted Reproduction. Parthenon Publishing Group. New York, NY (USA). Google Scholar

Language: Английский

Citations

22