Agronomía Mesoamericana,
Journal Year:
2023,
Volume and Issue:
unknown, P. 51957 - 51957
Published: Jan. 30, 2023
Introducción.
La
reproducción
asistida
de
animales
tiene
su
origen
en
la
domesticación
las
especies
ganaderas
útiles
al
ser
humano.
Los
consiguientes
procesos
selección
artificial
permitieron
desarrollar
técnicas
biotecnológicas
que
contribuyeron
a
mejora
capacidades
producción
animal.
Objetivo.
Contextualizar
el
análisis
seminal
agricultura
precisión
siglo
XXI.
Desarrollo.
visualización
espermatozoides
microscopio
puede
considerarse
como
primer
paso
advenimiento
biotecnología
y
base
para
desarrollo
asistida.
Con
perfeccionamiento
los
métodos
microscopía,
se
logró
caracterizar
gametos
masculinos,
lo
significó
un
avance
significativo
tecnología
inseminación
artificial.
El
punto
inflexión
marcado
por
conservación
espermática,
implicó
cambio
sustantivo
estas
tecnologías
ganaderas,
ya
sea
con
semen
crio
preservado
o
refrigerado.
Estos
son
alto
valor
caso
amenazadas,
dado
pueden
crear
bancos
germoplasma
propósitos
genética
rescate
riesgo
extinción.
ha
desarrollado
forma
paralela
asistida,
hoy
considera
una
técnica
relevante
animal,
perfeccionado
mediante
ciencia
tecnología,
física
óptica
computación.
Conclusión.
tenido
paradigma
desechar
obsoletas
evaluación
subjetiva
calidad
adoptar
objetivos
del
semen,
grandes
volúmenes
datos
variables
movilidad,
cinéticas,
morfométricas,
morfológicas
fragmentación
ADN,
permiten
mejor
eyaculados
reproductores
centros
Physiological Reviews,
Journal Year:
2021,
Volume and Issue:
102(1), P. 7 - 60
Published: April 21, 2021
The
spermatozoon
is
a
highly
differentiated
and
polarized
cell,
with
two
main
structures:
the
head,
containing
haploid
nucleus
acrosomal
exocytotic
granule,
flagellum,
which
generates
energy
propels
cell;
both
structures
are
connected
by
neck.
sperm’s
aim
to
participate
in
fertilization,
thus
activating
development.
Despite
this
common
bauplan
function,
there
an
enormous
diversity
structure
performance
of
sperm
cells.
For
example,
mammalian
spermatozoa
may
exhibit
several
head
patterns
overall
lengths
ranging
from
∼30
350
µm.
Mechanisms
transport
female
tract,
preparation
for
recognition
interaction
oocyte
also
show
considerable
variation.
There
has
been
much
interest
understanding
origin
diversity,
evolutionary
terms
relation
mechanisms
underlying
differentiation
testis.
Here,
relationships
between
function
examined
at
levels:
first,
analyzing
selective
forces
that
drive
changes
physiology
understand
adaptive
values
variation
impact
on
male
reproductive
success
second,
examining
cellular
molecular
formation
testis
explain
how
can
give
rise
such
wide
array
forms
functions.
Antioxidants,
Journal Year:
2023,
Volume and Issue:
12(9), P. 1673 - 1673
Published: Aug. 26, 2023
During
cryopreservation,
sperm
undergoes
structural
and
molecular
changes
such
as
ice
crystal
formation,
DNA
fragmentation,
reactive
oxygen
species
(ROS)
production,
leading
to
decreased
quality
after
thawing.
Antioxidants
play
a
crucial
role
in
preventing
these
damages,
both
vivo
vitro.
One
potent
antioxidant
is
myo-inositol,
known
for
its
protective
effects
on
against
ROS.
This
study
aimed
investigate
the
effect
of
myo-inositol
cryopreserved
boar
semen.
The
semen
was
diluted,
cooled,
using
BF5
extender.
It
then
divided
into
five
groups:
control
different
concentrations
(0.5,
1,
1.5,
2
mg/mL).
post-thaw
evaluation
included
assessments
motility,
viability,
acrosome
integrity,
mitochondrial
membrane
potential
(MMP),
caspase
activity,
gene
expression,
ROS
levels,
apoptosis,
IVF
with
treated
Results
showed
that
at
0.5
mg/mL
improved
fertilization
ability.
also
reduced
expression
pro-apoptotic
genes
increased
SMCP
expression.
Lower
demonstrated
viability
apoptosis
levels.
In
conclusion,
treatment
during
cryopreservation
quality,
enhanced
fertility
rates
Biology,
Journal Year:
2020,
Volume and Issue:
9(6), P. 138 - 138
Published: June 26, 2020
The
ejaculate
is
heterogenous
and
sperm
sub-populations
with
different
kinematic
patterns
can
be
identified
in
various
species.
Nevertheless,
although
these
are
statistically
well
defined,
the
statistical
differences
not
always
relevant.
aim
of
present
study
was
to
characterize
from
two
bovine
species,
diluted
commercial
extenders,
determine
relevance
through
Bayesian
analysis.
Semen
10
bulls
evaluated
after
thawing.
An
ISAS®v1
computer-assisted
analysis
(CASA)-Mot
system
employed
an
image
acquisition
rate
50
Hz
ISAS®D4C20
counting
chambers.
Sub-populations
motile
spermatozoa
were
characterized
using
multivariate
procedures
such
as
principal
components
(PCs)
clustering
methods
(k-means
model).
Four
three
PCs
that
involved
progressiveness,
velocity,
cell
undulatory
movement.
proportions
varied
extender
used
Despite
a
difference
(p
<
0.05)
between
confirmed
only
one
them
(Triladyl®)
presented
relevant
when
compared
Tris-EY
OptiXcell®.
Extenders
differed
proportion
cells
each
sub-populations.
Similar
Bos
taurus
indicus.
results
indicate
SP1,
SP2,
SP3
for
PC
criteria
For
linearity,
SP4
did
show
regarding
other
classical
approach
or
subpopulation
thus
may
have
direct
biological
meaning.
Therefore,
needs
reevaluated.
Animals,
Journal Year:
2021,
Volume and Issue:
11(4), P. 920 - 920
Published: March 24, 2021
The
aim
of
the
study
was
to
compare
morphometric
features
sperm
head
size
and
shape
from
Pietrain
line
Duroc
×
boar
crossbred
terminal
lines,
evaluate
their
relationship
with
reproductive
success
after
artificial
insemination
sows
produced
crossbreeding
York,
Landrace
breeds.
Semen
samples
were
collected
11
sexually
mature
boars.
Only
ejaculates
greater
than
70%
motility
rate
<15%
abnormal
used
for
inseminations
(AI)
included
in
study.
Samples
analyzed
using
an
ISAS®v1
computer-assisted
analysis
system
eight
parameters
(CASA-Morph).
Sub-populations
characterized
multivariate
procedures,
such
as
principal
component
(PC)
clustering
methods
(k-means
model).
Four
different
ejaculate
sub-populations
identified
two
PCs
that
involved
spermatozoa.
discriminant
ability
variables
predict
sow
litter
a
receiver
operating
characteristics
(ROC)
curve
analysis.
Sperm
length,
ellipticity,
elongation,
regularity
showed
significant
predictive
capacity
on
(0.59,
0.59,
0.60,
0.56
area
under
(AUC),
respectively).
not
related
size.
Journal of Animal and Feed Sciences,
Journal Year:
2020,
Volume and Issue:
29(3), P. 189 - 198
Published: Sept. 21, 2020
1.
Acosta
A.A.,
Kruger
T.F.
(Editors),
1996.
Human
Spermatozoa
in
Assisted
Reproduction.
Parthenon
Publishing
Group.
New
York,
NY
(USA).
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Scholar