Current Opinion in Structural Biology, Journal Year: 2019, Volume and Issue: 57, P. 1 - 8
Published: Feb. 25, 2019
Language: Английский
Current Opinion in Cell Biology, Journal Year: 2018, Volume and Issue: 53, P. 84 - 91
Published: July 2, 2018
Language: Английский
Citations
285
Chemical Reviews, Journal Year: 2018, Volume and Issue: 118(21), P. 10659 - 10709
Published: July 9, 2018
Sensory photoreceptors underpin light-dependent adaptations of organismal physiology, development, and behavior in nature. Adapted for optogenetics, sensory become genetically encoded actuators reporters to enable the noninvasive, spatiotemporally accurate reversible control by light cellular processes. Rooted a mechanistic understanding natural photoreceptors, artificial with customized light-gated function have been engineered that greatly expand scope optogenetics beyond original application light-controlled ion flow. As we survey presently, UV/blue-light-sensitive particularly allowed transcend its initial neuroscience applications unlocking numerous additional processes parameters optogenetic intervention, including gene expression, DNA recombination, subcellular localization, cytoskeleton dynamics, intracellular protein stability, signal transduction cascades, apoptosis, enzyme activity. The engineering novel benefits from powerful reusable design strategies, most importantly association (un)folding reactions. Additionally, modified versions these same serve as fluorescent proteins generators singlet oxygen, thereby further enriching toolkit. available upcoming detailed quantitative interrogation networks increasingly more precise illuminating manners.
Language: Английский
Citations
222
Biochemical Journal, Journal Year: 2019, Volume and Issue: 476(1), P. 1 - 23
Published: Jan. 7, 2019
Abstract Polyphosphoinositides (PPIs) are essential phospholipids located in the cytoplasmic leaflet of eukaryotic cell membranes. Despite contributing only a small fraction to bulk cellular phospholipids, they make remarkable contributions practically all aspects cell's life and death. They do so by recruiting proteins/effectors or interacting with domains membrane proteins at membrane–cytoplasm interface organize mold organelle identity. The present study summarizes our current understanding concerning metabolism, manipulation, measurement, intimate roles these lipids play regulating homeostasis vital signaling reactions health disease.
Language: Английский
Citations
214
Physiological Reviews, Journal Year: 2022, Volume and Issue: 102(3), P. 1263 - 1325
Published: Jan. 24, 2022
Optogenetics combines light and genetics to enable precise control of living cells, tissues, organisms with tailored functions. has the advantages noninvasiveness, rapid responsiveness, tunable reversibility, superior spatiotemporal resolution. Following initial discovery microbial opsins as light-actuated ion channels, a plethora naturally occurring or engineered photoreceptors photosensitive domains that respond at varying wavelengths ushered in next chapter optogenetics. Through protein engineering synthetic biology approaches, genetically encoded photoswitches can be modularly into scaffolds host cells myriad biological processes, well behavioral disease intervention vivo. Here, we summarize these optogenetic tools on basis their fundamental photochemical properties better inform chemical design principles. We also highlight exemplary applications opsin-free optogenetics dissecting cellular physiology (designated "optophysiology") describe current progress, future trends, wireless optogenetics, which enables remote interrogation physiological processes minimal invasiveness. This review is anticipated spark novel thoughts next-generation devices promise accelerate both basic translational studies.
Language: Английский
Citations
79
eLife, Journal Year: 2019, Volume and Issue: 8
Published: Nov. 14, 2019
Cholesterol is a major structural component of the plasma membrane (PM). The majority PM cholesterol forms complexes with other lipids, making it inaccessible for intracellular transport. Transition between accessible and pools maintains cellular homeostasis, but how cells monitor accessibility remains unclear. We show that endoplasmic reticulum (ER)-anchored lipid transfer proteins, GRAMD1s, sense transport to ER. GRAMD1s bind one another populate ER-PM contacts by sensing transient expansion pool via their GRAM domains. They then facilitate this StART-like Cells lack all three exhibit striking as result less efficient ER cholesterol. Thus, movement in order counteract an acute increase cholesterol, thereby activating non-vesicular
Language: Английский
Citations
136
Development, Journal Year: 2019, Volume and Issue: 146(20)
Published: Oct. 15, 2019
ABSTRACT The development of multicellular organisms is controlled by highly dynamic molecular and cellular processes organized in spatially restricted patterns. Recent advances optogenetics are allowing protein function to be with the precision a pulse laser light vivo, providing powerful new tool perturb developmental at wide range spatiotemporal scales. In this Primer, we describe most commonly used optogenetic tools, their application biology nascent field synthetic morphogenesis.
Language: Английский
Citations
102
Molecular Systems Biology, Journal Year: 2022, Volume and Issue: 18(6)
Published: June 1, 2022
Language: Английский
Citations
42
Frontiers in Microbiology, Journal Year: 2018, Volume and Issue: 9
Published: Nov. 8, 2018
Photo-receptors are widely present in both prokaryotic and eukaryotic cells, which serves as the foundation of tuning cell behaviors with light. While practices cells have been relatively established, trials bacterial only emerging past few years. A number light sensors engineered bacteria most them fall into categories two-component one-component systems. Such a sensor toolbox has enabled controlling synthetic circuits at level transcription protein activity is major topic biology according to central dogma. Additionally, served for achieving based real-time feedback control. Here we review programming light, introducing their applications, including controls over microbial culture.
Language: Английский
Citations
82
Cell Reports, Journal Year: 2020, Volume and Issue: 31(10), P. 107737 - 107737
Published: June 1, 2020
Spatially and temporally varying patterns of morphogen signals during development drive cell fate specification at the proper location time. However, current in vitro methods typically do not allow for precise, dynamic spatiotemporal control signaling are thus insufficient to readily study how dynamics affect behavior. Here, we show that optogenetic Wnt/β-catenin pathway activation can be controlled user-defined intensities, temporal sequences, spatial using engineered illumination devices photostimulation light variable amplitudes (LAVA). By patterning human embryonic stem (hESC) cultures with LAVA enabled dose-responsive optoWnt Brachyury expression. Furthermore, time-varying spatially localized revealed tissue models presentation Wnt vitro. provide a low-cost, user-friendly method high-throughput applications developmental biology.
Language: Английский
Citations
61
eLife, Journal Year: 2020, Volume and Issue: 9
Published: Nov. 11, 2020
Light-inducible dimerization protein modules enable precise temporal and spatial control of biological processes in non-invasive fashion. Among them, Magnets are small engineered from the Neurospora crassa photoreceptor Vivid by orthogonalizing homodimerization interface into complementary heterodimers. Both components, which well-tolerated as fusion partners, photoreceptors requiring simultaneous photoactivation to interact, enabling high spatiotemporal confinement with a single excitation wavelength. However, require concatemerization for efficient responses cell preincubation at 28°C be functional. Here we overcome these limitations engineering an optimized pair neither nor low temperature preincubation. We validated ‘enhanced’ (eMags) using them rapidly reversibly recruit proteins subcellular organelles, induce organelle contacts, reconstitute OSBP-VAP ER-Golgi tethering implicated phosphatidylinositol-4-phosphate transport metabolism. eMags represent very effective tool optogenetically manipulate physiological over whole cells or volumes.
Language: Английский
Citations
57