Wastewater surveillance for population-wide Covid-19: The present and future

The Science of The Total Environment, Journal Year: 2020, Volume and Issue: 736, P. 139631 - 139631

Published: May 23, 2020

Language: Английский

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COVID-19 surveillance in Southeastern Virginia using wastewater-based epidemiology

Water Research, Journal Year: 2020, Volume and Issue: 186, P. 116296 - 116296

Published: Aug. 13, 2020

Wastewater-based epidemiology (WBE) has been used to analyze markers in wastewater treatment plant (WWTP) influent characterize emerging chemicals, drug use patterns, or disease spread within communities. This approach can be particularly helpful understanding outbreaks of like the novel Coronavirus disease-19 (COVID-19) when combined with clinical datasets. In this study, three RT-ddPCR assays (N1, N2, N3) were detect severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA weekly samples from nine WWTPs southeastern Virginia. first several weeks sampling, SARS-CoV-2 detections sporadic. Frequency and overall concentrations increased mid March into late July. During twenty-one week ranged 101 104 copies 100 mL-1 where viral was detected. Fluctuations population normalized loading rates WWTP service areas agreed known during study. Here we propose ways that data presented spatially temporally greatest public health officials. As COVID-19 pandemic wanes, it is likely communities will see incidence small, localized outbreaks. these instances, WBE could as a pre-screening tool better target testing needs limited resources.

Language: Английский

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SARS-CoV-2 RNA in Wastewater Settled Solids Is Associated with COVID-19 Cases in a Large Urban Sewershed

Environmental Science & Technology, Journal Year: 2020, Volume and Issue: 55(1), P. 488 - 498

Published: Dec. 7, 2020

Wastewater-based epidemiology may be useful for informing public health response to viral diseases like COVID-19 caused by SARS-CoV-2. We quantified SARS-CoV-2 RNA in wastewater influent and primary settled solids two treatment plants inform the preanalytical analytical approaches assess whether or harbored more targets. The samples resulted higher detection frequencies than corresponding samples. Likewise, was readily detected using one-step digital droplet (dd)RT-PCR with two-step RT-QPCR ddRT-PCR, likely owing reduced inhibition ddRT-PCR assay. subsequently analyzed a longitudinal time series of 89 from single plant as well coronavirus recovery (bovine coronavirus) fecal strength (pepper mild mottle virus) controls. targets N1 N2 concentrations correlated positively significantly clinically confirmed case counts sewershed. Together, results demonstrate that measuring sensitive approach influent.

Language: Английский

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Correlation of SARS-CoV-2 RNA in wastewater with COVID-19 disease burden in sewersheds

The Science of The Total Environment, Journal Year: 2021, Volume and Issue: 775, P. 145790 - 145790

Published: Feb. 15, 2021

Language: Английский

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Tracking COVID-19 with wastewater

Nature Biotechnology, Journal Year: 2020, Volume and Issue: 38(10), P. 1151 - 1153

Published: Sept. 21, 2020

Language: Английский

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Reproducibility and sensitivity of 36 methods to quantify the SARS-CoV-2 genetic signal in raw wastewater: findings from an interlaboratory methods evaluation in the U.S.

Environmental Science Water Research & Technology, Journal Year: 2021, Volume and Issue: 7(3), P. 504 - 520

Published: Jan. 1, 2021

The reproducibility and sensitivity of 36 methods for quantifying the genetic signal SARS-CoV-2 in wastewater was evaluated a nationwide interlaboratory assessment U.S.

Language: Английский

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Sewage analysis as a tool for the COVID-19 pandemic response and management: the urgent need for optimised protocols for SARS-CoV-2 detection and quantification

Journal of environmental chemical engineering, Journal Year: 2020, Volume and Issue: 8(5), P. 104306 - 104306

Published: July 27, 2020

Language: Английский

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Making waves: Wastewater surveillance of SARS-CoV-2 for population-based health management

Water Research, Journal Year: 2020, Volume and Issue: 184, P. 116181 - 116181

Published: July 13, 2020

Language: Английский

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Wastewater surveillance to infer COVID-19 transmission: A systematic review

The Science of The Total Environment, Journal Year: 2021, Volume and Issue: 804, P. 150060 - 150060

Published: Sept. 8, 2021

Successful detection of SARS-COV-2 in wastewater suggests the potential utility wastewater-based epidemiology (WBE) for COVID-19 community surveillance. This systematic review aims to assess performance surveillance as early warning system transmission. A search was conducted PubMed, Medline, Embase and WBE Consortium Registry according PRISMA guidelines relevant articles published until 31st July 2021. Relevant data were extracted summarized. Quality each paper assessed using an assessment tool adapted from Bilotta et al.'s environmental science. Of 763 studies identified, 92 distributed across 34 countries shortlisted qualitative synthesis. total 26,197 samples collected between January 2020 May 2021 various locations serving population ranging 321 11,400,000 inhabitants. Overall sample positivity moderate at 29.2% all examined settings with spike (S) gene having maximum rate positive detections nucleocapsid (N) being most targeted. Wastewater signals preceded confirmed cases by up 63 days, 13 reporting before first detected community. At least 50 reported association viral load cases. While cannot replace large-scale diagnostic testing, it can complement clinical providing signs transmission more active public health responses. However, standardized validated methods are required along risk analysis modelling understand dynamics outbreaks.

Language: Английский

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Wastewater concentrations of human influenza, metapneumovirus, parainfluenza, respiratory syncytial virus, rhinovirus, and seasonal coronavirus nucleic-acids during the COVID-19 pandemic: a surveillance study

The Lancet Microbe, Journal Year: 2023, Volume and Issue: 4(5), P. e340 - e348

Published: March 22, 2023

Respiratory disease is a major cause of morbidity and mortality; however, surveillance for circulating respiratory viruses passive biased. Wastewater-based epidemiology has been used to understand SARS-CoV-2, influenza A, syncytial virus (RSV) infection rates at community level but not investigate other viruses. We aimed use wastewater-based viral occurrence.A retrospective study was carried out large wastewater treatment plant located in California, USA. Using droplet digital RT-PCR, we measured RNA concentrations A B viruses, RSV B, parainfluenza (1-4) rhinovirus, seasonal coronaviruses, metapneumovirus solids three times per week 17 months (216 samples) between Feb 1, 2021, June 21, 2022. Novel probe-based RT-PCR assays non-influenza targets were developed validated. compared positivity infections from clinical specimens submitted California Sentinel Clinical Laboratories (sentinel laboratories) assess concordance the two datasets.We detected all tested solids. Human rhinovirus (median concentration 4300 [0-9500] copies gram dry weight) human coronaviruses (35 000 [17 000-56 000]) found highest concentrations. Concentrations correlated significantly positively with associated diseases sentinel laboratories (tau 0·32-0·57, p<0·0009); only exceptions which rarely Measurements indicated coronavirus OC43 dominated whereas 3 among during period. decreased noticeably after omicron BA.1 surge suggesting connection changes behaviour transmission viruses.Wastewater-based can be obtain information on circulation localised, without need test many individuals because single sample represents entire contributing community. Results available within 24 h collection, generating real time inform public health responses, decision making, individual modifications.CDC Foundation.

Language: Английский

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