Antibiotics Alter Pocillopora Coral-Symbiodiniaceae-Bacteria Interactions and Cause Microbial Dysbiosis During Heat Stress DOI Creative Commons
Michael T. Connelly, Crystal J. McRae, Pi‐Jen Liu

et al.

Frontiers in Marine Science, Journal Year: 2022, Volume and Issue: 8

Published: Jan. 13, 2022

Symbioses between eukaryotes and their associated microbial communities are fundamental processes that affect organisms’ ecology evolution. A unique example of this is reef-building corals maintain symbiotic associations with dinoflagellate algae (Symbiodiniaceae) bacteria coral health through various mechanisms. However, little understood about how coral-associated holobiont heat tolerance. In study, we investigated these interactions in four Pocillopora colonies belonging to three cryptic species by subjecting fragments treatments antibiotics intended suppress the normal community, followed acute stress. Separate only or stress were conducted compare effects individual stressors on transcriptome responses microbiome shifts. Across all examined, combined treatment significantly altered caused major changes both Cladocopium algal symbiont gene expression. Individually, impaired protein translation activated DNA repair processes, while downregulation amino acid inorganic ion transport metabolism genes photosynthesis genes. Combined antibiotics-heat synergistic expression including enhanced oxidative response genes, programed cell death pathways proteolytic enzymes indicate an exacerbated following community suppression. Collectively, results provide further evidence Symbiodiniaceae engage highly coordinated metabolic crucial for health, homeostasis,

Language: Английский

Genetic approaches for increasing fitness in endangered species DOI
Tiffany A. Kosch, Anthony W. Waddle, Caitlin Cooper

et al.

Trends in Ecology & Evolution, Journal Year: 2022, Volume and Issue: 37(4), P. 332 - 345

Published: Jan. 10, 2022

Language: Английский

Citations

40
Clustered Regularly Interspaced Short Palindromic Repeat/CRISPR-Associated Protein and Its Utility All at Sea: Status, Challenges, and Prospects DOI Creative Commons
Jiashun Li,

Shuaishuai Wu,

Kaidian Zhang

et al.

Microorganisms, Journal Year: 2024, Volume and Issue: 12(1), P. 118 - 118

Published: Jan. 6, 2024

Initially discovered over 35 years ago in the bacterium Escherichia coli as a defense system against invasion of viral (or other exogenous) DNA into genome, CRISPR/Cas has ushered new era functional genetics and served versatile genetic tool all branches life science. revolutionized methodology gene knockout with simplicity rapidity, but it is also powerful for knock-in modification. In field marine biology ecology, this been instrumental characterization ‘dark’ genes documentation differentiation paralogs. Powerful is, challenges exist that have hindered advances some important lineages. This review examines status applications research assesses prospect quickly expanding deployment to address myriad fundamental biological oceanography questions.

Language: Английский

Citations

11
Advancing the protection of marine life through genomics DOI Creative Commons
Madeleine J. H. van Oppen, Melinda A. Coleman

PLoS Biology, Journal Year: 2022, Volume and Issue: 20(10), P. e3001801 - e3001801

Published: Oct. 17, 2022

The rapid growth in genomic techniques provides the potential to transform how we protect, manage, and conserve marine life. Further, solutions boost resilience of species climate change other disturbances that characterize Anthropocene require transformative approaches, made more effective if guided by data. Although genetic have been employed conservation for decades availability data is rapidly expanding, widespread application still lags behind types. This Essay reviews genetics genomics utilized management initiatives ocean restoration, highlights success stories, presents a pathway forward enhance uptake protecting our oceans.

Language: Английский

Citations

36
Harnessing the omics revolution to address the global biodiversity crisis DOI
Luis F. De León, Bruna Silva, Kevin J. Avilés‐Rodríguez

et al.

Current Opinion in Biotechnology, Journal Year: 2023, Volume and Issue: 80, P. 102901 - 102901

Published: Feb. 10, 2023

Language: Английский

Citations

19
Management approaches to conserve Australia’s marine ecosystem under climate change DOI Open Access
Line K. Bay, James Gilmour, Bob Muir

et al.

Science, Journal Year: 2023, Volume and Issue: 381(6658), P. 631 - 636

Published: Aug. 10, 2023

Australia's coastal marine ecosystems have a deep cultural significance to Indigenous Australians, include multiple World Heritage sites, and support the nation's rapidly growing blue economy. Yet, increasing local pressures global climate change are expected undermine biological, social, cultural, economic value of these within human generation. Mitigating causes is most urgent action secure their future; however, conventional new management actions will play roles in preserving ecosystem function until that achieved. This includes strategies codeveloped with Australians guided by traditional ecological knowledge modeling decision framework. We provide examples developments at one iconic ecosystems, Great Barrier Reef, where recent, large block funding supports research, governance, engagement accelerate development tools for under change.

Language: Английский

Citations

19
Finding genes and pathways that underlie coral adaptation DOI Creative Commons
Oliver Selmoni, Line K. Bay, Moisés Expósito‐Alonso

et al.

Trends in Genetics, Journal Year: 2024, Volume and Issue: 40(3), P. 213 - 227

Published: Feb. 6, 2024

Language: Английский

Citations

8
Toward bio‐optical phenotyping of reef‐forming corals using Light‐Induced Fluorescence Transient‐Fast Repetition Rate fluorometry DOI Creative Commons
David J. Suggett, Matthew R. Nitschke, David Hughes

et al.

Limnology and Oceanography Methods, Journal Year: 2022, Volume and Issue: 20(3), P. 172 - 191

Published: Jan. 27, 2022

Abstract Active chlorophyll a fluorometry is well‐established tool for noninvasively diagnosing coral functional state, but has not yet been developed as rapid phenotyping (functional screening) platform agriculture and forestry. Here, we present proof‐of‐concept using Light‐Induced Fluorescence Transient‐Fast Repetition Rate (LIFT‐FRRf) to identify photobiological‐based phenotypes in the context of rapidly scaling propagation practices on northern Great Barrier Reef. For example, resolving light niche plasticity inform transplantation, identifying functionally diverse colonies maximize stock selection. We first used optically laboratory‐reared corals endosymbiont (Symbiodiniaceae) isolates develop approach integrating FRRf instantaneous kinetic parameters (light harvesting, electron turnover rates) light‐dependent (dynamic “quenching” terms, saturating intensity [ E K ]). Subsequent field‐based LIFT‐FRRf from selective (2‐4 m depth) reef habitat revealed that widely topographically dispersed plating Acropora taxa exhibited broad ( variance) underpinned by multiple were predominantly differentiated minimum capacity; fluorometer configurations cannot resolve will thus likely have more limited capacity phenotypes. As such, potential terms variants across environments (growth, transplantation). In contrast, Pocillopora verrucosa , Echinopora lamellosa ) with relatively restricted topographic dispersion less only single phenotypes, thereby imposing constraints propagation. discuss core technical, operational, conceptual steps required sophisticated platforms.

Language: Английский

Citations

23
Unlocking the Complex Cell Biology of Coral–Dinoflagellate Symbiosis: A Model Systems Approach DOI Creative Commons
Marie R. Jacobovitz, Elizabeth A. Hambleton, Annika Guse

et al.

Annual Review of Genetics, Journal Year: 2023, Volume and Issue: 57(1), P. 411 - 434

Published: Sept. 19, 2023

Symbiotic interactions occur in all domains of life, providing organisms with resources to adapt new habitats. A prime example is the endosymbiosis between corals and photosynthetic dinoflagellates. Eukaryotic dinoflagellate symbionts reside inside coral cells transfer essential nutrients their hosts, driving productivity most biodiverse marine ecosystem. Recent advances molecular genomic characterization have revealed symbiosis-specific genes mechanisms shared among symbiotic cnidarians. In this review, we focus on cellular processes that underpin interaction symbiont host. We discuss acquisition via phagocytosis, modulation host innate immunity, integration into cell metabolism, nutrient exchange as a fundamental aspect stable associations. emphasize importance using model systems dissect complexity endosymbiosis, which ultimately serves basis for understanding its ecology capacity face climate change.

Language: Английский

Citations

15
Intentional introgression of a blight tolerance transgene to rescue the remnant population of American chestnut DOI Creative Commons
Andrew E. Newhouse, William A. Powell

Conservation Science and Practice, Journal Year: 2020, Volume and Issue: 3(4)

Published: Dec. 31, 2020

Abstract In contrast to many current applications of biotechnology, the intended consequence American Chestnut Research & Restoration Project is produce trees that are well‐adapted thrive not just in confined fields or orchards, but throughout their natural range. Our primary focus on disease tolerance, we believe it will also be critically important optimal restoration should have robust genetic diversity and resilience, which can supplied by a full complement wild‐type genes. offers unique case study because intervention options been attempted: doing nothing, planting non‐native chestnut species, hybrids, mutagenesis (exposing seeds high levels radiation induce random mutations), backcross breeding, now engineering. Any these techniques may advantageous independently combinations, depending specific goals land managers practitioners, engineering opportunity enhance blight tolerance while minimizing other changes genome.

Language: Английский

Citations

33
Nanobiotech engineering for future coral reefs DOI Creative Commons
Liza M. Roger, Nastassja A. Lewinski, Hollie M. Putnam

et al.

One Earth, Journal Year: 2023, Volume and Issue: 6(7), P. 778 - 789

Published: June 5, 2023

Advances in bioengineering and nanotechnology are revolutionizing how we approach problems deemed unsolvable only a decade ago. Nanotechnology has transformed biomedicine, agriculture, energy science, with broad translational capacity to natural systems. Coral reef ecosystems provide immense biodiversity economic value but being degraded at an unprecedented rate, triggering calls for human interventions such as those that have been applied biomedical Here, propose next-generation nanobiotechnology (nanocarriers, nanobiosensors, 3D bioprinting) can be leveraged solutions the persistence of future reefs. We advocate initiating critical dialogues developing tools apply coral ecosystems. challenge invite scientific community industry harness expand available toolkits monitoring, rehabilitation, restoration, conservation reefs worldwide.

Language: Английский

Citations

12