Effects of Xenorhabdus and Photorhabdus bacterial metabolites on the ovipositional activity of Aedes albopictus DOI Creative Commons
Mustapha Touray, Harun Çimen, Edna Bode

et al.

Research Square (Research Square), Journal Year: 2023, Volume and Issue: unknown

Published: Oct. 11, 2023

Abstract Viral diseases like yellow fever, dengue, zika have an alarming impact on public health affecting millions of people around the globe. These can be transmitted by Aedes albopictus – a species originally endemic to South Asia but now widely distributed in several countries outside Asia. Xenorhabdus and Photorhabdus spp., are enteric Gram-negative bacterial symbionts insect-preying nematodes known produce array natural products (NP) with various activities including larvicidal activity. This study demonstrated that supernatants different bacteria effectively deterred Ae. mosquito oviposition concentration dependent manner. cabanillasii displayed deterrent effects at 50-5% concentrations activity index values ranging between -0.87 -0.35, whereas for X. nematophila were -0.82 -0.52 50-10%. The other ( szentirmaii, doucetiae, P. kayaii ) effective ≥20%. Using promoter exchange mutants selected biosynthetic gene clusters Δ hfq background generated easyPACId approach, fabclavine from szentirmaii was identified as bioactive compound evident effects. Such compounds deterrence could useful control programs preventing breeding site specific habitats greatly influence establishment, population densities, dispersion conducive areas.

Language: Английский

Structural basis for sugar perception by Drosophila gustatory receptors DOI
Demin Ma, Meiqin Hu, Xiaotong Yang

et al.

Science, Journal Year: 2024, Volume and Issue: 383(6685)

Published: Feb. 2, 2024

Insects rely on a family of seven transmembrane proteins called gustatory receptors (GRs) to encode different taste modalities, such as sweet and bitter. We report structures

Language: Английский

Citations

25
Repellent efficacy of 20 essential oils on Aedes aegypti mosquitoes and Ixodes scapularis ticks in contact-repellency assays DOI Creative Commons
Hailey A. Luker,

Keyla R. Salas,

Delaram Esmaeili

et al.

Scientific Reports, Journal Year: 2023, Volume and Issue: 13(1)

Published: Jan. 30, 2023

Cases of mosquito- and tick-borne diseases are rising worldwide. Repellent products can protect individual users from being infected by such diseases. In a previous study, we identified five essential oils that display long-distance mosquito repellency using Y-tube olfactometer assay. the current contact repellent efficacy 20 active ingredients Environmental Protection Agency's (EPA) Minimum Risk Pesticides list were tested Aedes aegypti Ixodes scapularis. We utilized an arm-in-cage assay to measure complete protection time bites for these ingredients. To tick repellency, used EPA-recommended procedure crossings. found tested, 10% v/v lotion emulsions with clove oil or cinnamon provided longest both conclude in emulsion, specific EPA provide crossings longer than one hour.

Language: Английский

Citations

25
Discrete roles of Ir76b ionotropic coreceptor impact olfaction, blood feeding, and mating in the malaria vector mosquito Anopheles coluzzii DOI Creative Commons
Zi Ye,

Feng Liu,

Huahua Sun

et al.

Proceedings of the National Academy of Sciences, Journal Year: 2022, Volume and Issue: 119(23)

Published: June 1, 2022

Significance Chemosensory systems play crucial roles across mosquito life cycles, with underlying molecular receptors often forming functional complexes that require cognate coreceptors. To better understand chemosensory pathways in the malaria vector Anopheles coluzzii , gene editing were used to localize and functionally characterize ionotropic receptor coreceptor AcIr76b . Expression of was observed antennal grooved pegs, coeloconic sensilla, other accessory olfactory appendages. When inactivated, mutant mosquitoes displayed altered neuronal responses amines.. Beyond phenotypes, mutants display significantly impaired mating blood feeding capabilities. Our data reveal discrete pathways, suggesting as targets for design control strategies.

Language: Английский

Citations

30
Arbovirus impact on mosquito behavior: the jury is still out DOI Creative Commons
Théo Maire, Louis Lambrechts, Felix J.H. Hol

et al.

Trends in Parasitology, Journal Year: 2024, Volume and Issue: 40(4), P. 292 - 301

Published: Feb. 28, 2024

Language: Английский

Citations

7
The complexities of blood‐feeding patterns in mosquitoes and sandflies and the burden of disease: A minireview DOI Creative Commons
Fatma Bursalı, Mustapha Touray

Veterinary Medicine and Science, Journal Year: 2024, Volume and Issue: 10(5)

Published: Aug. 22, 2024

Mosquitoes and sandflies exhibit a wide range of blood feeding patterns, targeting vertebrate species, including birds, mammals, reptiles, amphibians, for proteins vital egg development. This broad host increases the opportunity them to acquire pathogens numerous debilitating-and-fatal diseases from various animal reservoirs, playing significant role in disease crossover between animals humans, also known as zoonotic transmission. review focuses on intricate blood-feeding habits these dipteran vectors, their sensory systems complex dance pathogen during We delve into influence sources spread by examining insect immune response its interplay with pathogens. The remarkable sense smell guiding towards food hosts is explored, highlighting multiple cues navigation. Finally, we examine challenges mosquito control strategies explore innovations this field, emphasizing need sustainable solutions combat global health threat. By understanding biology behaviour insects, can develop more effective protect ourselves mitigate burden vector-borne diseases.

Language: Английский

Citations

7
Microorganisms Associated with Mosquito Oviposition Sites: Implications for Habitat Selection and Insect Life Histories DOI Creative Commons
Maxime Girard,

Edwige Martin,

Laurent Vallon

et al.

Microorganisms, Journal Year: 2021, Volume and Issue: 9(8), P. 1589 - 1589

Published: July 26, 2021

Mosquitoes are considered one of the most important threats worldwide due to their ability vector pathogens. They responsible for transmission major pathogens such as malaria, dengue, zika, or chikungunya. Due lack treatments prophylaxis against many transmitted and an increasing prevalence mosquito resistance insecticides drugs available, alternative strategies now being explored. Some these involve use microorganisms promising agent limit fitness mosquitoes, attract repel them, decrease replication pathogenic agents. In recent years, importance colonizing habitat mosquitoes has particularly been investigated since they appeared play roles in development diseases transmission. this issue, we will synthesize researches investigating how present within water habitats may influence breeding site selection oviposition gravid females. We also highlight impact microbes on fate females' progeny during immature stages with a specific focus egg hatching, rate, larvae pupae survival.

Language: Английский

Citations

34
Neurogenetic identification of mosquito sensory neurons DOI Creative Commons
Joanna K. Konopka, Darya Task, Danny Poinapen

et al.

iScience, Journal Year: 2023, Volume and Issue: 26(5), P. 106690 - 106690

Published: April 19, 2023

Anopheles mosquitoes, as vectors for the malaria parasite, are a global threat to human health. To find and bite human, they utilize neurons within their sensory appendages. However, identity quantification of appendage lacking. Here we use neurogenetic approach label all in coluzzii mosquitoes. We homology assisted CRISPR knock-in (HACK) generate T2A-QF2w synaptic gene bruchpilot. membrane-targeted GFP reporter visualize brain quantify major chemosensory appendages (antenna, maxillary palp, labella, tarsi, ovipositor). By comparing labeling brp>GFP Orco>GFP predict extent expressing ionotropic receptors (IRs) or other receptors. This work introduces valuable genetic tool functional analysis mosquito neurobiology initiates characterization that guide behavior.

Language: Английский

Citations

13
Effects of Xenorhabdus and Photorhabdus bacterial metabolites on the ovipositional activity of Aedes albopictus DOI Creative Commons
Mustapha Touray, Harun Çimen, Edna Bode

et al.

Journal of Pest Science, Journal Year: 2024, Volume and Issue: 97(4), P. 2203 - 2215

Published: March 21, 2024

Abstract Viral diseases like yellow fever, dengue, and Zika have an alarming impact on public health. These can be transmitted by Aedes mosquito species, such as Ae. albopictus, which is now found in many countries outside its original range. Xenorhabdus Photorhabdus spp. are enteric bacterial symbionts of insect-preying nematodes known to produce array natural products with various activities including larvicidal activity. In this study, the effects produced four one bacteria ovipositional behavior albopictus mosquitoes were assessed. Utilizing a binary choice assay insect cages, gravid female presented two oviposition cups containing water supplemented varying concentrations supernatants (50–1% concentrations) versus control medium. After 72 h, eggs deposited filter papers counted. The attractant index (OAI) feature supernatant was evaluated using number laid cups. Notably, all tested exhibited concentration-dependent deterrence oviposition. cabanillasii displayed strongest deterrent effect, inhibiting egg-laying at 50–5% (OAI: − 0.87 0.35), followed X. nematophila (50–10%, OAI: 0.82 0.52). szentirmaii, doucetiae, P. kayaii showed significant ≥ 20% concentrations. Using promoter exchange mutants generated easyPACId approach, fabclavine from szentirmaii identified bioactive compound evident effects. Such deterrents targeting could valuable for controlling populations disrupting their breeding suitable habitats.

Language: Английский

Citations

4
Olfactory preferences and chemical differences of fruit scents for Aedes aegypti mosquitoes DOI Creative Commons

Melissa Leon-Noreña,

Genevieve M. Tauxe,

Jeffrey A. Riffell

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 6, 2025

Abstract Feeding on the sugar of fruits and flowers is vital for mosquitoes increases their lifespan, reproduction, flight activity. Olfaction a key sensory modality in mediating mosquito responses to sources. Previous studies have demonstrated that natural nectar sources from can vary attractiveness mosquitoes, with some preferred over others. However, how different relates chemical composition scent they evoke mosquito’s peripheral olfactory system, still not understood. In this study, we use closely related fruit species varieties examine changes chemistry influence fruit’s Aedes aegypti (via electroantennogram recordings, or EAG) correlate those differences. Our results show are attracted scents certain ( Mangifera indica, Prunus perspica, Psidium guajava, Musa acuminata ), whereas others Pyrus communis, Citrus limon ) elicited significantly negative control. Chemical analyses showed attractive distinct profiles, amongst fruits, minor relative proportions compounds modify attractiveness. These differences were reflected EAG responses, which similar varieties. Experimentally altering proportion single compound caused significant decrease attraction levels less cultivars. demonstrate sensitive odors, implications processing complex odor sources, like plants blood hosts. Summary Statement specific selective preferences scent.

Language: Английский

Citations

0
Making scents of mosquito repellents DOI
Cole J. Meier, Minh N. Nguyen, Christopher J. Potter

et al.

Trends in Parasitology, Journal Year: 2025, Volume and Issue: unknown

Published: March 1, 2025

Language: Английский

Citations

0