Reversible Fluorescent Probes for Dynamic Imaging of Liver Ischemia-Reperfusion Injury DOI
Wen Zhang, Jihong Liu, Ping Li

et al.

Accounts of Chemical Research, Journal Year: 2024, Volume and Issue: 57(17), P. 2594 - 2605

Published: Aug. 20, 2024

ConspectusHepatic ischemia-reperfusion injury (HIRI) is an inevitable complication of clinical surgeries such as liver resection or transplantation, often resulting in postoperative dysfunction, hepatic failure up to 13% postresection patients, and early graft 11-18% transplantation patients. HIRI involves a series biochemical events triggered by abnormal alterations multiple biomarkers, characterized short lifespans, dynamic changes, subcellular regional distribution, multicollaborative regulation. However, traditional diagnosis, including serology, imaging, puncture biopsy, suffers from low sensitivity, poor resolution, hysteresis, which hinder effective monitoring markers. Thus, address the unique properties markers, there pressing demand for developing novel detection strategies that are highly selective, transiently responsive, dynamically reversible, organelle-targeted, capable simultaneous multicomponent analysis.Optical probe-based fluorescence imaging powerful tool real-time biomarkers with advantages high noninvasiveness, rapid analysis, high-fidelity acquisition spatiotemporal information on signaling molecules compared conventional methods. Moreover, growing continuous probes reversible features receiving more attention. Importantly, can not only monitor fluctuations marker concentrations but also distinguish between transient bursts markers during physiological long-term sustained increases pathological levels. This effectively avoid false-positive test results, addition, be reutilized green economical features. Therefore, our team has employed various methods design optical HIRI. We proposed recognition based specific reactions interactions detect changes Given biomarkers' organelles synergistic regulatory HIRI, bifunctional exploited, organelle-targeted detection. With these strategies, we have tailored variety fluorescent reactive oxygen/nitrogen species (O

Language: Английский

ROS are evolutionary conserved cell-to-cell stress signals DOI Creative Commons
Yosef Fichman, Linda Rowland, Melvin J. Oliver

et al.

Proceedings of the National Academy of Sciences, Journal Year: 2023, Volume and Issue: 120(31)

Published: July 26, 2023

Cell-to-cell communication is fundamental to multicellular organisms and unicellular living in a microbiome. It thought have evolved as stress- or quorum-sensing mechanism organisms. A unique cell-to-cell that uses reactive oxygen species (ROS) signal (termed the “ROS wave”) was identified flowering plants. This process essential for systemic signaling plant acclimation stress can spread from small group of cells entire within minutes. Whether similar found other however unknown. Here, we report ROS wave be algae, amoeba, ferns, mosses, mammalian cells, isolated hearts. We further show this triggered by local H 2 O treatment blocked application catalase NADPH oxidase inhibitors algae it communicates important stress–response signals between cells. Taken together, our findings suggest an active signaling, like wave, before diverged. could communicated environmental coordinated response many different community. The finding process, contributes understanding diseases impact development drugs target example cancer heart disease.

Language: Английский

Citations

34
Stimuli‐responsive linkers and their application in molecular imaging DOI Creative Commons
Jing Wang,

Meng Liu,

Xinyue Zhang

et al.

Exploration, Journal Year: 2024, Volume and Issue: 4(4)

Published: Jan. 18, 2024

Molecular imaging is a non-invasive method that widely used for visualization and detection of biological events at cellular or molecular levels. Stimuli-responsive linkers can be selectively cleaved by specific biomarkers desired sites to release activate agents are appealing tools improve the specificity, sensitivity, efficacy imaging. This review summarizes recent advances stimuli-responsive their application in imaging, highlighting potential these design activatable probes. It hoped this could inspire more research interests development responsive associated applications.

Language: Английский

Citations

15
In Situ Visualizing Carboxylesterase Activity in Type 2 Diabetes Mellitus Using an Activatable Endoplasmic Reticulum Targetable Proximity Labeling Far-Red Fluorescent Probe DOI

Ningge Xu,

Dandan Tang, Heng Liu

et al.

Analytical Chemistry, Journal Year: 2024, Volume and Issue: 96(26), P. 10724 - 10731

Published: June 19, 2024

Carboxylesterase (CE), an enzyme widely present in organisms, is involved various physiological and pathological processes. Changes the levels of CEs liver may predict presence type 2 diabetes mellitus (T2DM). Here, a novel dicyanoisophorone (DCI)-based proximity-labeled far-red fluorescent probe DCI2F-Ac with endoplasmic reticulum targeting was proposed for real-time monitoring imaging activity. featured very low cytotoxicity biotoxicity highly selective sensitive CEs. Compared traditional probes, covalently anchored directly to CEs, thus effectively reducing loss situ signals due diffusion. Through "on–off" fluorescence signal readout, able distinguish cell lines screen inhibitors. In terms (ER) stress, it found that thapsigargin (Tg) induced upregulation but not tunicamycin (Tm), which related calcium homeostasis ER. could efficiently detect downregulated livers T2DM, therapeutic efficacy metformin, acarbose, combination these two drugs assessed by tracking fluctuation levels. The results showed combining metformin acarbose restore near-normal best antidiabetic effect. Thus, provides great opportunity explore untapped potential metabolic disorders drug assessment.

Language: Английский

Citations

9
Fluorogenic and Mitochondria-Localizable Probe Enables Selective Labeling and Imaging of Nitroreductase DOI
Shuyi Wang, Wei Tan,

Wenjie Lang

et al.

Analytical Chemistry, Journal Year: 2022, Volume and Issue: 94(20), P. 7272 - 7277

Published: May 12, 2022

Nitroreductase (NTR), one of the flavin-dependent enzymes and an upregulated enzyme under tumor hypoxia, has been studied for decades. Many fluorescent probes were developed to detect NTR activity; however, these tend diffuse away from their reaction site (NTR) inevitably, leading inappropriate sample fixation, lower accuracy localization, weaker signal-to-noise ratio. Herein, we present design, synthesis, in vitro evaluation, biological applications NTR-activatable fluorogenic labeling probe FY. By integrating with quinone methide (QM) proximity-based protein labeling, additional fluoromethyl group on FY serves as a potential origin QM. Compared conventional probes, this new not only offers mitochondrial localizable response but also achieves permanent retention activation enhanced spatial resolution improve detection sensitivity even after cell fixation. We believe our work could offer expandable synthetic approach develop imaging fluorescence deciphering complex events.

Language: Английский

Citations

34
Activity-Based Self-Enriched SERS Sensor for Blood Metabolite Monitoring DOI

Chenlei Cai,

Yujie Liu, Zheng Zhang

et al.

ACS Applied Materials & Interfaces, Journal Year: 2023, Volume and Issue: 15(4), P. 4895 - 4902

Published: Jan. 23, 2023

The monitoring of metabolites in biofluids provides critical clues for disease diagnosis and evaluation. Yet, the quantitative detection remains challenging surface-enhanced Raman spectroscopy (SERS) due to poor reproducibility preparation manipulation SERS nanoprobes. Herein, we develop an activity-based, slippery liquid-infused porous surface (abSLIPSERS) sensor facile quantification with unmodified naked metal nanoparticles (NPs) by integrating biocatalysis-boronate oxidation cascades SLIPS-driven self-concentration delivering. Upon mixing target metabolite a specific oxidase, H2O2-sensitive phenylboronate probe, Au NPs, H2O2 produced from biocatalytic reaction oxidizes probe phenol, resulting ratiometric response. Meanwhile, SLIPS enables complete enrichment molecules NPs within evaporating liquid droplet, delivering probes SERS-active sites amplification. Compared conventional biosensors, abSLIPSERS avoids multistep synthesis biofunctionalization nanoprobes, which significantly simplifies workflow improves reproducibility. also shows tunable dynamic range beyond 4 orders magnitude allows quantifying any other enzymes. We demonstrate sensing lactate, glucose, choline human serum exploring energy metabolism lung cancer. This study opens up new opportunity future point-of-care testing circulating will help facilitate translation bioanalysis clinical settings.

Language: Английский

Citations

21
Fluorescence-Based Detection of Fatty Acid β-Oxidation in Cells and Tissues Using Quinone Methide-Releasing Probes DOI
Shohei Uchinomiya,

Tomoki Nagaura,

Mark Weber

et al.

Journal of the American Chemical Society, Journal Year: 2023, Volume and Issue: 145(14), P. 8248 - 8260

Published: April 3, 2023

Detection of metabolic activity enables us to reveal the inherent state cells and elucidate mechanisms underlying cellular homeostasis growth. However, a fluorescence approach for study pathways is still largely unexplored. Herein, we have developed new chemical probe fluorescence-based detection fatty acid β-oxidation (FAO), key process in lipid catabolism, tissues. This serves as substrate FAO forms reactive quinone methide (QM) result reactions. The liberated QM covalently captured by intracellular proteins, subsequent bio-orthogonal ligation with fluorophore analysis. reaction-based sensing allowed detect at desired emission wavelength using diverse analytical techniques including imaging, in-gel activity-based protein profiling (ABPP), fluorescence-activated cell sorting (FACS). was able changes induced modulators cultured cells. further employed imaging mouse liver tissues revealed heterogeneity hepatocytes combination FACS gene expression analysis, highlighting utility our tool metabolism research.

Language: Английский

Citations

20
Electron‐withdrawing inductive effects enhanced strategy for protein thiol sensing and blocking agent design DOI Creative Commons
Liangwei Zhang, Shudi Liu, Xia Zhang

et al.

Smart Molecules, Journal Year: 2024, Volume and Issue: 2(1)

Published: Feb. 14, 2024

Abstract It is a great challenge to discover novel chemical reactions suitable for biological analysis in living system. The development of protein thiol blocking agents crucial need exploring functions refolding, signal transduction, and redox regulation. We are always keen on seeking applied endogenous macromolecules or sensing, blocking, labeling. In the present work, we have successfully developed agent block by enhanced electron‐withdrawing inductive effects. This sensing process was detailedly monitored UV‐ vis , fluorescent spectra, SDS‐Page gel separation. spectral studies demonstrated that could react ultrafastly with within seconds at μM level. Furthermore, imaging cells vivo further used validation its ability thiol, providing evidence downregulated thiols Parkinson's disease. effect strategy this work may provide general guideline designing agent.

Language: Английский

Citations

7
Activity-Based Sensing for Chemistry-Enabled Biology: Illuminating Principles, Probes, and Prospects for Boronate Reagents for Studying Hydrogen Peroxide DOI Creative Commons
Marco S. Messina, Gianluca Quargnali, Christopher J. Chang

et al.

ACS Bio & Med Chem Au, Journal Year: 2022, Volume and Issue: 2(6), P. 548 - 564

Published: Oct. 11, 2022

Activity-based sensing (ABS) offers a general approach that exploits chemical reactivity as method for selective detection and manipulation of biological analytes. Here, we illustrate the value this platform to enable new discovery through case study in design application ABS reagents studying hydrogen peroxide (H

Language: Английский

Citations

28
Unique assembly of carbonylpyridinium and chromene reveals mitochondrial thiol starvation under ferroptosis and novel ferroptosis inducer DOI Creative Commons
Kaiqing Ma, He Yang, Tianruo Shen

et al.

Chemical Science, Journal Year: 2022, Volume and Issue: 13(13), P. 3706 - 3712

Published: Jan. 1, 2022

To reveal the delicate function of mitochondria, spatiotemporally precise detection tools remain highly desirable. However, current probes with positively charged warheads for targeting mitochondria diffuse out as potential mitochondrial membrane changes, which directly influences accuracy detection. Herein, we assembled carbonylpyridinium and chromene to afford probe CM-Mit. Following ultrafast response thiol dissociation carbonylpyridinium, formation o-quinone methide from CM-Mit was proposed label proteins, thus avoiding diffusion mitochondria. Therefore, accurate spatiotemporal in realized. With this excellent probe, ferroptosis inducers were proved stimulate starvation first time cancer cells. Moreover, used screen a compound library developed in-house stemona alkaloid analog SA-11 shown induce various cell lines, including drug-resistant one.

Language: Английский

Citations

26
Microenvironment‐Tailored Catalytic Nanoprobe for Ratiometric NIR‐II Fluorescence/Photoacoustic Imaging of H2O2 in Tumor and Lymphatic Metastasis DOI
Tao Chen, Zhaolin Chen,

Qianting Zhou

et al.

Advanced Functional Materials, Journal Year: 2022, Volume and Issue: 32(47)

Published: Sept. 20, 2022

Abstract In vivo H 2 O visualization is crucial for disease diagnosis. Catalytic reaction‐based probes show potential in detection, yet their application remains challenging because catalysts always require a specific pH to function and cellular glutathione (GSH) may suppress signaling by depletion of hydroxyl radicals oxidized substrates. Here, microenvironment‐tailored catalytic nanoprobe (MTCN) comprising Fe 2+ , citric acid (CA), 2,2′‐azino‐bis(3‐ethylbenzothiazoline‐6‐sulfonic acid) diammonium salt (ABTS), downconversion nanoparticles the liposomal cavity as well reference dye lipid membrane reported, which utilizes selective permeability offer favorable catalyst with aid CA avoid GSH‐triggered signal loss preventing entry GSH into cavity. The MTCN displays large NIR‐II fluorescence (FL) ratio between 1550 1080 nm (FL 1550Em,808Ex /FL 1080Em,980Ex ), but small photoacoustic (PA) 808 1048 (PA /PA ). Upon exposure conversion ABTS its form ABTS· + absorption causes noticeable increment PA accompanied an apparent decrement FL enabling bimodal ratiometric imaging tumor lymphatic metastasis.

Language: Английский

Citations

23