Juvenile hormone controls trehalose metabolism by regulating trehalase 2 activity in ovarian development of Helicoverpa armigera DOI Open Access
Q. Qin, Bo Zhang, Bin Fang

et al.

Insect Molecular Biology, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 6, 2024

Trehalase (Treh) is crucial for ovarian development as it directly regulates the energy supply by hydrolyzing trehalose into glucose. Juvenile hormone (JH) also essential development, but how affects Treh2 activity remains unclear. This study, which employed Helicoverpa armigera a model, showed that HaTreh2 transcription and enzymatic peaks coincided with peak of JH titers (the 2 3 days after emergence). Compared to dsGFP control, knockdown severely impaired development. LC-MS/MS site mutation experiments demonstrated triggered serine 345 phosphorylation via GPCR-cAMP-PKA pathway, thereby activating its activity. Additionally, bound transporter (HaTreT) under induction, thus controlling intracellular glucose contents well HaTreT. TreT controls amount trehalose, serves substrate Treh1, entering cell. Treh2, on other hand, uses extracellular substrate, hydrolysis product further transported Here, regulated HaTreh1 can act upon in cell binding HaTreT during when induced. Therefore, systematically metabolism through regulating HaTreh2. study sheds light coordinated interplay between pathway sugar

Language: Английский

Nanoparticle-Shielded dsRNA Delivery for Enhancing RNAi Efficiency in Cotton Spotted Bollworm Earias vittella (Lepidoptera: Nolidae) DOI Open Access

Shelja Sandal,

Satnam Singh,

Gulshan Bansal

et al.

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(11), P. 9161 - 9161

Published: May 23, 2023

The spotted bollworm Earias vittella (Lepidoptera: Nolidae) is a polyphagous pest with enormous economic significance, primarily affecting cotton and okra. However, the lack of gene sequence information on this has significant constraint molecular investigations formulation superior management strategies. An RNA-seq-based transcriptome study was conducted to alleviate such limitations, de novo assembly performed obtain transcript sequences pest. Reference identification across E. developmental stages RNAi treatments were using its information, which resulted in identifying transcription elongation factor (TEF), V-type proton ATPase (V-ATPase), Glyceraldehyde -3-phosphate dehydrogenase (GAPDH) as most suitable reference genes for normalization RT-qPCR-based expression studies. present also identified important developmental, pathway, target life-stage analysis RT-qPCR select optimal targets RNAi. We found that naked dsRNA degradation hemolymph primary reason poor A total six including Juvenile hormone methyl transferase (JHAMT), Chitin synthase (CHS), Aminopeptidase (AMN), Cadherin (CAD), Alpha-amylase (AMY), (V-ATPase) selected knocked down significantly three different nanoparticles encapsulated conjugates, i.e., Chitosan-dsRNA, carbon quantum dots-dsRNA (CQD-dsRNA), Lipofectamine-dsRNA conjugate. These results demonstrate feeding nanoparticle-shielded silences suggests nanoparticle-based can efficiently manage

Language: Английский

Citations

24
Endocrine factors modulating vitellogenesis and oogenesis in insects: An update DOI
Jimena Leyria

Molecular and Cellular Endocrinology, Journal Year: 2024, Volume and Issue: 587, P. 112211 - 112211

Published: March 15, 2024

Language: Английский

Citations

14
GPCR–MAPK signaling pathways underpin fitness trade-offs in whitefly DOI Creative Commons
Buli Fu,

Jinjin Liang,

Jinyu Hu

et al.

Proceedings of the National Academy of Sciences, Journal Year: 2024, Volume and Issue: 121(28)

Published: July 3, 2024

Trade-offs between evolutionary gain and loss are prevalent in nature, yet their genetic basis is not well resolved. The evolution of insect resistance to insecticide often associated with strong fitness costs; however, how the trade-offs operates remains poorly understood. Here, we show that mitogen-activated protein kinase (MAPK) pathway its upstream downstream actors underlie whitefly

Language: Английский

Citations

12
Pesticide-induced resurgence in brown planthopper is mediated by action on a suite of genes that promote juvenile hormone biosynthesis and female fecundity DOI Open Access
Yang Gao,

Shao-Cong Su,

Xing Jun-wei

et al.

Published: Feb. 26, 2025

Pesticide-induced resurgence, increases in pest insect populations following pesticide application, is a serious threat to the sustainable control of many highly damaging crop pests. Resurgence can result from pesticide-enhanced reproduction, however, molecular mechanisms mediating this process remain unresolved. Here we show that brown planthopper (BPH) resurgence rice crops exposure emamectin benzoate (EB) results coordinated action suite genes regulate juvenile hormone (JH) levels, resulting increased JH titer adult females and enhanced fecundity. We demonstrate EB treatment at sublethal levels profound changes female BPH fitness including egg maturation oviposition. This reproductive EB-mediated upregulation key involved regulation JH, JHAMT Kr-h1 downregulation allatostatin ( AstA ) receptor AstAR expression. signaling known inhibit production corpora allata hence diminishes inhibitory action. find gene expression are caused by insecticide on its target, glutamate-gated chloride channel (GluCl). Collectively, these provide mechanistic insights into negative pesticide-induced responses insects reveal actors JH-signaling pathway underpin resurgence.

Language: Английский

Citations

1
Juvenile hormone promotes paracellular transport of yolk proteins via remodeling zonula adherens at tricellular junctions in the follicular epithelium DOI Creative Commons
Hongyuan Zheng, Ningbo Wang,

Jiaqi Yun

et al.

PLoS Genetics, Journal Year: 2022, Volume and Issue: 18(6), P. e1010292 - e1010292

Published: June 27, 2022

Juvenile hormone (JH) acts as a gonadotrophic stimulating insect vitellogenesis and oogenesis. Paracellular transport of yolk proteins through intercellular channels (patency) in the follicular epithelium is developmentally regulated evolutionarily conserved process during vitellogenesis. However, mechanisms underlying patency opening are poorly understood. Using migratory locust Locusta migratoria model system, we report here that JH-regulated remodeling zonula adherens (ZA), belt-like junction maintaining physical linking between follicle cells controlled patency. JH triggered phosphorylation Partitioning defective protein 3 (Par3) via signaling cascade including G protein-coupled receptor (GPCR), small GTPase Cell division cycle 42 (Cdc42) atypical Protein kinase C (aPKC). Par3 resulted its disassociation from β-Catenin, cytoplasmic partner ZA core component E-Cadherin. Release β-Catenin/E-Cadherin complex caused disassembly at tricellular contacts, consequently leading to enlargement. This study provides new insight into how stimulates egg production inducing paracellular route for vitellogenin crossing barrier.

Language: Английский

Citations

31
Biological Characteristics and Energy Metabolism of Migrating Insects DOI Creative Commons
Xiaokang Li, Yan Zhou, Kongming Wu

et al.

Metabolites, Journal Year: 2023, Volume and Issue: 13(3), P. 439 - 439

Published: March 17, 2023

Through long-distance migration, insects not only find suitable breeding locations and increase the survival space opportunities for population but also facilitate large-scale material, energy, information flow between regions, which is important in maintaining stability of agricultural ecosystems wider natural ecosystems. In this study, we summarize changes biological characteristics such as morphology, ovarian development, reproduction, flight capability during seasonal migration insect. consideration global research work, interaction influence regulation insulin-like juvenile hormone on reproductive activities migrating insects, types energy substances, metabolic processes, processes insect are elaborated. This systematic review latest advances studies biology metabolism will help readers to better understand behavior mechanism migration.

Language: Английский

Citations

15
Differential regulation of reproduction and molting by juvenile hormone in aphids DOI
Haolin Li,

Xue Kong,

Dongyu Yang

et al.

Journal of Insect Physiology, Journal Year: 2025, Volume and Issue: unknown, P. 104791 - 104791

Published: March 1, 2025

Language: Английский

Citations

0
Receptor tyrosine kinases CAD96CA and FGFR1 function as the cell membrane receptors of insect juvenile hormone DOI Open Access
Yan-Xue Li, Xin-Le Kang, Yan-Li Li

et al.

Published: March 3, 2025

Juvenile hormone (JH) is important to maintain insect larval status; however, its cell membrane receptor has not been identified. Using the lepidopteran Helicoverpa armigera (cotton bollworm), a serious agricultural pest, as model, we determined that tyrosine kinases (RTKs) cadherin 96ca (CAD96CA) and fibroblast growth factor homologue (FGFR1) function JH receptors by their roles in JH-regulated gene expression, status maintaining, rapid intracellular calcium increase, phosphorylation of MET1 cofactor Taiman, high affinity III. Gene knockout Cad96ca Fgfr1 CRISPR/Cas9 embryo knockdown various cells, overexpression CAD96CA FGFR1 mammalian HEK-293T cells all supported transmitting signal receptors.

Language: Английский

Citations

0
Receptor tyrosine kinases CAD96CA and FGFR1 function as the cell membrane receptors of insect juvenile hormone DOI Creative Commons
Yan-Xue Li, Xin-Le Kang, Yan-Li Li

et al.

eLife, Journal Year: 2025, Volume and Issue: 13

Published: March 14, 2025

Juvenile hormone (JH) is important to maintain insect larval status; however, its cell membrane receptor has not been identified. Using the lepidopteran Helicoverpa armigera (cotton bollworm), a serious agricultural pest, as model, we determined that tyrosine kinases (RTKs) cadherin 96ca (CAD96CA) and fibroblast growth factor homologue (FGFR1) function JH receptors by their roles in JH-regulated gene expression, status maintaining, rapid intracellular calcium increase, phosphorylation of MET1 cofactor Taiman, high affinity III. Gene knockout Cad96ca Fgfr1 CRISPR/Cas9 embryo knockdown various cells, overexpression CAD96CA FGFR1 mammalian HEK-293T cells all supported transmitting signal receptors.

Language: Английский

Citations

0
Chitin Deacetylase Gene Family Positively Regulates the Accumulation of Rice Stripe Virus in Laodelphax striatellus Fallén (Hemiptera: Delphacidae) Ovaries DOI Creative Commons
Wenxing Hu,

Ao You,

Jiao Zhang

et al.

Insects, Journal Year: 2025, Volume and Issue: 16(4), P. 334 - 334

Published: March 22, 2025

Chitin deacetylase modifies chitin and has critical functions in the growth development of insects; however, whether it other roles is unclear. Laodelphax striatellus not only directly feeds on phloem sap rice but also transmits RSV, leading to significant losses production. In this study, four CDA genes were identified based SBPH genomic data classified into groups I III a phylogenetic analysis. The expression LsCDA1 LsCDA2 RSV-infected was increased by 282% 159%, respectively, relative non-infected control. Additionally, RSV enhanced (increased 194%) ovaries. Yeast two-hybrid glutathione-S-transferase pull-down assays demonstrated interaction between proteins NP NS2. Furthermore, knockdown decreased viral loads its ovaries 66% 72% dsGFP Silencing significantly VgR reduced fecundity. These results indicate that positively regulates accumulation reproduction modulating expression, which offers novel strategy for controlling both targeting LsCDA1.

Language: Английский

Citations

0