Pesticide Biochemistry and Physiology, Journal Year: 2024, Volume and Issue: 204, P. 106058 - 106058
Published: July 31, 2024
Language: Английский
Current Biology, Journal Year: 2024, Volume and Issue: 34(3), P. 505 - 518.e6
Published: Jan. 11, 2024
Germ cells are essential to sexual reproduction. Across the animal kingdom, extracellular signaling isoprenoids, such as retinoic acids (RAs) in vertebrates and juvenile hormones (JHs) invertebrates, facilitate multiple processes Here we investigated role of these potent molecules embryonic germ cell development, using JHs Drosophila melanogaster a model system. In contrast their established endocrine roles during larval adult germline found that JH acts locally development. Using an vivo biosensor, observed active first within near primordial (PGCs) they migrate developing gonad. Through vitro assays, determined both necessary sufficient for PGC migration. Analysis into mechanisms this newly uncovered paracrine function revealed migration was compromised when were decreased or increased, suggesting specific titers spatiotemporal dynamics required robust colonization Compromised can impair fertility cause tumors many species, including humans. mammals, retinoids have development We like Drosophila, RA impact mouse vitro. Together, our study reveals previously unanticipated isoprenoids local effectors pre-gonadal suggests broadly shared mechanism
Language: Английский
Citations
19
Molecular and Cellular Endocrinology, Journal Year: 2024, Volume and Issue: 587, P. 112211 - 112211
Published: March 15, 2024
Language: Английский
Citations
14
Published: Feb. 26, 2025
Pesticide-induced resurgence, increases in pest insect populations following pesticide application, is a serious threat to the sustainable control of many highly damaging crop pests. Resurgence can result from pesticide-enhanced reproduction, however, molecular mechanisms mediating this process remain unresolved. Here we show that brown planthopper (BPH) resurgence rice crops exposure emamectin benzoate (EB) results coordinated action suite genes regulate juvenile hormone (JH) levels, resulting increased JH titer adult females and enhanced fecundity. We demonstrate EB treatment at sublethal levels profound changes female BPH fitness including egg maturation oviposition. This reproductive EB-mediated upregulation key involved regulation JH, JHAMT Kr-h1 downregulation allatostatin ( AstA ) receptor AstAR expression. signaling known inhibit production corpora allata hence diminishes inhibitory action. find gene expression are caused by insecticide on its target, glutamate-gated chloride channel (GluCl). Collectively, these provide mechanistic insights into negative pesticide-induced responses insects reveal actors JH-signaling pathway underpin resurgence.
Language: Английский
Citations
1
Metabolites, Journal Year: 2023, Volume and Issue: 13(3), P. 439 - 439
Published: March 17, 2023
Through long-distance migration, insects not only find suitable breeding locations and increase the survival space opportunities for population but also facilitate large-scale material, energy, information flow between regions, which is important in maintaining stability of agricultural ecosystems wider natural ecosystems. In this study, we summarize changes biological characteristics such as morphology, ovarian development, reproduction, flight capability during seasonal migration insect. consideration global research work, interaction influence regulation insulin-like juvenile hormone on reproductive activities migrating insects, types energy substances, metabolic processes, processes insect are elaborated. This systematic review latest advances studies biology metabolism will help readers to better understand behavior mechanism migration.
Language: Английский
Citations
15
PLoS Pathogens, Journal Year: 2023, Volume and Issue: 19(12), P. e1011859 - e1011859
Published: Dec. 7, 2023
Microsporidia are a group of obligate intracellular parasites that infect almost all animals, causing serious human diseases and major economic losses to the farming industry. Nosema bombycis is typical microsporidium infects multiple lepidopteran insects via fecal-oral transovarial transmission (TOT); however, underlying TOT processes mechanisms remain unknown. Here, we characterized process identified key factors enabling N . invade ovariole oocyte silkworm Bombyx mori We found commenced with at early pupal stage when ovarioles penetrated ovary wall were exposed hemolymph. Subsequently, in hemolymph cells firstly infiltrated sheath, from where they invaded two routes: (I) infecting follicular cells, thereby penetrating oocytes after proliferation, (II) nurse thus entering following replication. In follicle restructured built large vacuoles deliver themselves into oocyte. whole process, coated B vitellogenin (BmVg) on their surfaces. To investigate BmVg effects TOT, suppressed its expression dramatic decrease pathogen load both eggs, suggesting plays crucial role TOT. Thereby, domains parasite spore proteins (SWPs) mediating interaction, demonstrated von Willebrand domain (VWD) interacted SWP12, SWP26 SWP30, unknown function (DUF1943) bound SWP30. When disrupting these interactions, significant reductions interplays between SWPs vital for conclusion, our study has elucidated aspects about microsporidian revealed understanding molecular this transmission.
Language: Английский
Citations
12
Bioagro, Journal Year: 2025, Volume and Issue: 37(1), P. 13 - 24
Published: Jan. 1, 2025
Pyriproxyfen, a juvenile hormone analog (JHA), is considered as reduced-risk alternative to synthetic pesticides for crop protection. It has been frequently used in agriculture and public health manage insect pests. However, recent studies have reported that pyriproxyfen may adverse physiological effects on non-target organisms. This study investigated the of sublethal doses endocrine disrupting insecticide Drosophila melanogaster Meigen (Diptera: Drosophilidae) biological model. Results showed had noticeable effect developmental stages individuals exposed generation. Pyriproxyfen treatment significantly shortens adult longevity both sexes, female male. Finally, these results suggest reproduction capacity D. impacted by reducing number progeny after parent’s generation with pyriproxyfen. These research findings indicate exposure induces affects offspring growth rates insects Drosophila.
Language: Английский
Citations
0
Published: March 3, 2025
Juvenile hormone (JH) is important to maintain insect larval status; however, its cell membrane receptor has not been identified. Using the lepidopteran Helicoverpa armigera (cotton bollworm), a serious agricultural pest, as model, we determined that tyrosine kinases (RTKs) cadherin 96ca (CAD96CA) and fibroblast growth factor homologue (FGFR1) function JH receptors by their roles in JH-regulated gene expression, status maintaining, rapid intracellular calcium increase, phosphorylation of MET1 cofactor Taiman, high affinity III. Gene knockout Cad96ca Fgfr1 CRISPR/Cas9 embryo knockdown various cells, overexpression CAD96CA FGFR1 mammalian HEK-293T cells all supported transmitting signal receptors.
Language: Английский
Citations
0
eLife, Journal Year: 2025, Volume and Issue: 13
Published: March 14, 2025
Juvenile hormone (JH) is important to maintain insect larval status; however, its cell membrane receptor has not been identified. Using the lepidopteran Helicoverpa armigera (cotton bollworm), a serious agricultural pest, as model, we determined that tyrosine kinases (RTKs) cadherin 96ca (CAD96CA) and fibroblast growth factor homologue (FGFR1) function JH receptors by their roles in JH-regulated gene expression, status maintaining, rapid intracellular calcium increase, phosphorylation of MET1 cofactor Taiman, high affinity III. Gene knockout Cad96ca Fgfr1 CRISPR/Cas9 embryo knockdown various cells, overexpression CAD96CA FGFR1 mammalian HEK-293T cells all supported transmitting signal receptors.
Language: Английский
Citations
0
Journal of Advanced Research, Journal Year: 2025, Volume and Issue: unknown
Published: April 1, 2025
Language: Английский
Citations
0
Microbiome, Journal Year: 2025, Volume and Issue: 13(1)
Published: May 23, 2025
The insect gut microbiota forms a complex, multifunctional system that significantly affects phenotypic traits linked to environmental adaptation. Strong reproductive potential underpins the migratory success, population growth and destructive impact of fall armyworm, Spodoptera frugiperda (J.E. Smith). However, precise role bacteria in S. processes, distribution transmission dynamics remains unclear. We examined major invasive agricultural pest, identifying Enterococcus, Enterobacter, Klebsiella as core microorganisms present throughout its life cycle. These microbes showed heightened activity during egg stage, early larval stages pre-oviposition period females. Using an axenic re-infection system, Enterococcus quebecensis FAW181, michiganensis FAW071 Enterobacter hormaechei FAW049 were found enhance host fecundity, increasing production by 62.73%, 59.95%, 56.71%, respectively. Metagenomic haemolymph metabolomic analyses revealed positive correlation between symbiotic hormone metabolism female frugiperda. Further analysis metabolites biosynthesis pathway, along with exogenous injection juvenile III 20-hydroxyecdysone, regulate these hormones, maintaining levels equivalent those control insects. This regulation supports improved fecundity frugiperda, aiding rapid colonization expansion. findings emphasize pivotal E. FAW049, K. enhancing reproduction modulating JH through JHAMT concurrently 20E precursors via PHM. Our results provide novel insights into microbe-host symbiosis pest management strategies for alien species. Video Abstract.
Language: Английский
Citations
0