Unearthing lights-induced epilithic bacteria community assembly and associated function potentials in historical relics DOI Open Access
Wenjing Liu, Yuanyuan Bao, Jianwei Zhang

et al.

International Biodeterioration & Biodegradation, Journal Year: 2023, Volume and Issue: 186, P. 105701 - 105701

Published: Oct. 27, 2023

Language: Английский

Diversity and composition of microbial communities in Jinsha earthen site under different degree of deterioration DOI
Jing Li,

Yanqiu He,

Changjie He

et al.

Environmental Research, Journal Year: 2023, Volume and Issue: 242, P. 117675 - 117675

Published: Nov. 19, 2023

Language: Английский

Citations

8

The biodeterioration outbreak in Dunhuang Mogao Grottoes analyzed for the microbial communities and the occurrence time by C-14 dating DOI
Wenxia Ma, Fasi Wu, Dongpeng He

et al.

International Biodeterioration & Biodegradation, Journal Year: 2022, Volume and Issue: 178, P. 105533 - 105533

Published: Dec. 5, 2022

Language: Английский

Citations

13

Preservation status and microbial community of waterlogged archaeological woods over 7800 years old at the Jingtoushan Site, China DOI
Yang Lu,

Lichao Jiao,

Guoping Sun

et al.

Wood Science and Technology, Journal Year: 2023, Volume and Issue: 57(2), P. 537 - 556

Published: March 1, 2023

Language: Английский

Citations

7

Exploring the key deteriorative microorganisms on ancient ivories unearthed from the Sanxingdui Ruins site during temporary cold storage DOI Creative Commons

Guangjie Lao,

Zhiwei Zhou,

Rao Wu

et al.

Frontiers in Microbiology, Journal Year: 2024, Volume and Issue: 15

Published: April 16, 2024

Introduction The ancient ivories unearthed from the Sanxingdui Ruins site are valuable cultural relics, however, microbial biodeterioration on during temporary cold storage poses a great threat to their later long-term preservation. Methods Here, combination of high-throughput sequencing and biochemical assays was applied for in-depth investigation key deteriorative microorganisms colonizing tracing origin, as well assessment ethanol disinfection impact communities ivories. Results It observed that surfaces were scattered by fungal patches white, dark grey, hedge green colors storage. results showed genera Mortierella (38.51%), Ilyonectria (14.43%), Penicillium (1.15%), Aspergillus (1.09%) dominant fungi, while Pseudomonas (22.63%), Sphingopyxis (3.06%), Perlucidibaca (2.92%) bacteria isolated A-2 resulted in highest amount calcium releasing degradation hydroxyapatite (HAP), main component ivory, organic acids produced, including oxalic acid citric acid. fast expectation-maximization source tracking (FEAST) analysis revealed majority fungi (57.45%) (71.84%) derived soils surrounding sacrifice pits, indicating primary spoilage microbes growing strains could degrade cellulose, components wet cotton towels commonly moisture maintenance, aiding Notably, ivory with 75% cleansing significantly decreased relative abundance , becoming one Discussion Together, particularly played significant role producing acids. These may improve control develop more efficient strategies long-time conservation other relics.

Language: Английский

Citations

2

Fauna-microbe diversity coupling lost in agricultural soils: Implications from the bacteria hidden in earthworm gut DOI Creative Commons

Xin Gong,

Sibo Shi,

Xianping Li

et al.

Fundamental Research, Journal Year: 2023, Volume and Issue: unknown

Published: April 1, 2023

Earthworms are critical for supporting soil health and microbial diversity simultaneously maintaining a highly diverse gut microbiome. The earthworm is predominantly vulnerable to physical disturbance, yet how changes in influence the richness ecological network of soil-gut microbiomes response anthropogenic disturbance virtually unknown. Here, we investigated earthworms, their connection with microbiome using large-scale survey covering paired agricultural natural sites. Our results showed that was positively correlated bacterial across However, between lost ecosystems. We further show supported greater modularity networks, being both bacteria land-use types. Together, provided first empirical evidence practices can break fundamental links diversity, identify an unreported consistent earthworms networks managed These findings emphasize primary roles biodiversity point wider contributions animal-microbe interactions preserving whole anthropogenically disturbed

Language: Английский

Citations

5

Identification of microbial diversity in buried ivory soil at the Sanxingdui site in Guanghan City, China, using high-throughput sequencing DOI Creative Commons
Siyu Sun, Zhe Xu, Mengjia Ren

et al.

Frontiers in Microbiology, Journal Year: 2024, Volume and Issue: 15

Published: May 30, 2024

Introduction The Sanxingdui Site in Guanghan City, Sichuan Province, China, is one of the precious heritage sites ancient Chinese civilization. Archaeological work at great significance clarifying origins and main contents Shu culture Yangtze River Since 1920s, archaeologists have conducted extensive excavations research site, with particular attention given to large number ivory artifacts unearthed. However, buried influenced by soil pH, temperature, humidity, other physical chemical factors, along potential impact microbial activities that may lead corrosion decomposition ivory. By understanding types microorganisms, appropriate measures can be taken protect preserve cultural relics. Methods Multi-point sampling samples around three sacrificial pits site was carried out, strict aseptic operation out during process. Subsequently, community structure diversity were identified analyzed Illumina high-throughput sequencing technology. Results 16S rRNA internal transcribed spacer sequence analysis revealed significant differences among different pits. dominant bacterial phyla Proteobacteria, GAL15, Actinobacteriota, Bacteroidota, Methylomirabilota. fungal Ascomycota, Mortierellomhcota, Basidiomycota. Most communities play an indispensable role mechanism, promoting decay process through various means such as decomposing organic matter producing acidic substances. Discussion It particularly important take a series control activity effectively Our preliminary study mechanism action microorganisms on environment provides scientific basis prevent against degradation unearthed Sanxingdui. Following results, suitable antibacterial agents tailored preservation characteristics prepared. Ensure selected meet safety effectiveness requirements maximize protection

Language: Английский

Citations

1

Future Perspectives on the Application of the Oxford Nanopore® MinION™ Sequencer in Cultural Heritage Biodeterioration Studies DOI Open Access
João Trovão, Fabiana Soares

Published: June 21, 2024

The present work provides an updated overview of the application Oxford Nanopore® MinION™ sequencer in cultural heritage biodeterioration studies, while also providing a holistic discussion possible future perspectives for their utilization this research field. Due to peculiar characteristics device, last few years have seen steady rise on system variety materials, having been useful understand microbial biodeteriogens diversity and some metabolic biodeteriogenic features. Considering immense potential system, manuscript discusses further possibilities technique aiming help critical questions area. Its various differential contexts, has opened doors putative usage other interesting sub-areas worthy investigations, including: genome transcriptome analysis, metatranscriptomics, biodeteriorative metabolism inter intra kingdom interactions resistome profiling, object history context situ applications, bioprospecting biotechnology.

Language: Английский

Citations

1

Future Perspectives on the Application of the Oxford Nanopore® MinION™ Sequencer in Cultural Heritage Biodeterioration Studies DOI Open Access
João Trovão, Fabiana Soares

Published: July 15, 2024

The present work provides an updated overview of the application Oxford Nanopore® MinION™ sequencer in cultural heritage biodeterioration studies, while also providing a holistic discussion possible future perspectives for their utilization this research field. Due to peculiar characteristics device, last few years have seen steady rise on system variety materials, having been useful understand microbial biodeteriogens diversity and some metabolic biodeteriogenic features. Considering immense potential system, manuscript discusses further possibilities technique aiming help critical questions area. Its various differential contexts, has opened doors putative usage other interesting sub-areas worthy investigations, including: genome transcriptome analysis, metatranscriptomics, biodeteriorative metabolism inter intra kingdom interactions resistome profiling, object history context situ applications, bioprospecting biotechnology.

Language: Английский

Citations

1

Study on the Influence of Electron Beam Radiation Sterilization Method on Chinese Mural Pigment DOI Open Access
Min Luo, Bo Peng, Yang Shao

et al.

Processes, Journal Year: 2023, Volume and Issue: 11(5), P. 1403 - 1403

Published: May 5, 2023

Murals are one of the important cultural heritages mankind. The microbial control murals is an subject in mural painting conservation. In recent years, electron beam radiation sterilization has attracted more and attention field relic protection. immovable relics, so conventional irradiation equipment can not be used. However, development small mobile shows potential radiation’s application protection relics such as murals. A feasibility study paintings needed to investigate effect influence dose on stability pigments bonding materials. this paper, effects typical bacteria tomb mineral pigment powder ancient Chinese were studied a laboratory. Firstly, aeromonas hydrophila (Aer.h) penicillium flavigenum (PNC) selected representative strains determine appropriate for Then, seven kinds white calcium carbonate ground layer verified. results follows: 10 kGy effectively remove two strains. This will cause color difference lead white, while other essentially stable. Based UV-vis intensities four samples, change them increased with increasing up 30 kGy, after which signs saturation began appear. X-ray diffraction (XRD), Raman spectra, photoelectron spectroscopy (XPS) absorption near edge structure (XANES) spectra showed that chemical samples did irradiation. formation free radicals treated was confirmed using paramagnetic resonance (EPR) spectrum test. According all characterization results, between may due combination unpaired electrons defects process form centers. After forming center, light changed. reversible change, but take long time return their original state. focuses composition, preliminary exploration whether applied paintings, experimental provide basic data later application.

Language: Английский

Citations

3

Wheat genome architecture influences interactions with phytobeneficial microbial functional groups in the rhizosphere DOI Open Access
Cécile Gruet, Danis Abrouk, Andreas Börner

et al.

Plant Cell & Environment, Journal Year: 2022, Volume and Issue: 46(3), P. 1018 - 1032

Published: Dec. 10, 2022

Wheat has undergone a complex evolutionary history, which led to allopolyploidization and the hexaploid bread wheat Triticum aestivum. However, significance of genomic architecture for beneficial plant-microbe interactions is poorly understood, especially from functional standpoint. In this study, we tested hypothesis that was an overriding factor determining root recruitment microorganisms with particular plant-beneficial traits. We chose five species representing profiles AA (Triticum urartu), BB {SS} (Aegilops speltoides), DD tauschii), AABB dicoccon) AABBDD aestivum) assessed by quantitative polymerase chain reaction their ability interact free-nitrogen fixers, 1-aminocyclopropane-1-carboxylate deaminase producers, 2,4-diacetylphloroglucinol producers auxin via phenylpyruvate decarboxylase pathway, in combination Illumina MiSeq metabarcoding analysis N fixers (and total bacterial community). found abundance microbial groups could fluctuate according profile, as did abundance. fixer diversity were also influenced significantly profile. Often, rather similar results obtained genomes (Ae. tauschii) (T. aestivum), pointing first time D genome be particularly important wheat-bacteria interactions.

Language: Английский

Citations

5