De novo genome assemblies of two cryptodiran turtles with ZZ/ZW and XX/XY sex chromosomes provide insights into patterns of genome reshuffling and uncover novel 3D genome folding in amniotes DOI Creative Commons
Basanta Bista, Laura González-Rodelas, Lucía Álvarez-González

et al.

Genome Research, Journal Year: 2024, Volume and Issue: 34(10), P. 1553 - 1569

Published: Oct. 1, 2024

Understanding the evolution of chromatin conformation among species is fundamental to elucidate architecture and plasticity genomes. Nonrandom interactions linearly distant loci regulate gene function in species-specific patterns, affecting genome function, evolution, and, ultimately, speciation. Yet, data from nonmodel organisms are scarce. To capture macroevolutionary diversity vertebrate conformation, here we generate de novo assemblies for two cryptodiran (hidden-neck) turtles via Illumina sequencing, chromosome capture, RNA-seq:

Language: Английский

Genetic variation among sea turtle life stages and species suggests connectivity among ocean basins DOI Creative Commons
Katrina F. Phillips, Katherine R. Martin, Gustavo D. Stahelin

et al.

Ecology and Evolution, Journal Year: 2022, Volume and Issue: 12(11)

Published: Oct. 30, 2022

Abstract Regional genetic differentiation of mitochondrial lineages occurs in migratory species with natal philopatry such as sea turtles. However, early juvenile dispersal represents a key opportunity for gene flow and colonization new regions through founder events, making it an important yet under‐studied life stage. To assess connectivity among turtle stages ocean basins, we sequenced DNA (mtDNA) fragments from 35 juveniles sampled the Gulf Mexico rarely observed stage across three species: green turtles ( Chelonia mydas ; n = 30), hawksbills Eretmochelys imbricata 3), loggerheads Caretta caretta 2). We estimated rookery contributions using many‐to‐many Bayesian mixed stock analysis that incorporated probabilities based on size transport via currents. assembled tree including 709 distinct mtDNA control region haplotypes literature all seven extant to gaps life‐stage data well contextualize dispersing juveniles. Our results indicate high likelihood originated rookeries along coast Mexico, smaller Costa Rica Suriname. The yielded species‐level relationships consistent those presented previously, while intra‐species between basins differed, particularly within loggerhead clades. highlight lack turtles, especially stage, potential these answer broader questions diversification lineages.

Language: Английский

Citations

6
Histological features and Gαolf expression patterns in the nasal cavity of sea turtles DOI Open Access
Daisuke Kondoh,

Yuka Kaneoya,

Wataru Tonomori

et al.

Journal of Anatomy, Journal Year: 2023, Volume and Issue: 243(3), P. 486 - 503

Published: April 12, 2023

Sea turtles use olfaction to detect volatile and water-soluble substances. The nasal cavity of green (Chelonia mydas) comprises morphologically defined the anterodorsal, anteroventral, posterodorsal diverticula, as well a single posteroventral fossa. Here, we detailed histological features mature female turtle. diverticulum contained spongy-like venous sinuses wave-shaped sensory epithelium that favored ventilation. Secretory structures were significant in non-sensory epithelia probably involved protection against seawater. These findings suggested efficiently intake airborne substances dissolve mucous, while suppressing effects salts. In addition, positive staining Gαs/olf couples with olfactory, but not vomeronasal, receptors was predominant all three types cavity. Both odorants seemed be detected cells expressing Gαolf olfactory receptors.

Language: Английский

Citations

3
A chromosome-level genome assembly of the Asian house martin implies potential genes associated with the feathered-foot trait DOI Creative Commons
Yuan-Fu Chan, Chia‐Wei Lu, Hao‐Chih Kuo

et al.

G3 Genes Genomes Genetics, Journal Year: 2024, Volume and Issue: 14(6)

Published: April 12, 2024

The presence of feathers is a vital characteristic among birds, yet most modern birds had no feather on their feet. discoveries the hind limbs basal and dinosaurs have sparked an interest in evolutionary origin genetic mechanism feathered However, majority studies investigating genes associated with this trait focused domestic populations. Understanding underpinned feathered-foot development wild still its infancy. Here, we assembled chromosome-level genome Asian house martin (Delichon dasypus) using long-read High Fidelity sequencing approach to initiate search for We employed whole-genome alignment D. dasypus other swallow species identify high-SNP regions chromosomal inversions genome. After filtering out variations unrelated evolution, found six related near regions. also detected three between barn genomes. discussed association wingless/integrated (WNT), bone morphogenetic protein, fibroblast growth factor pathways potential roles development. Future are encouraged utilize explore process avian species. This endeavor will shed light path birds.

Language: Английский

Citations

0
The molecular arsenal of the key coastal bioturbator Hediste diversicolor faced with changing oceans DOI Creative Commons
Kaylee Beine, Lauric Feugere, Nichola Fletcher

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 24, 2024

Abstract The importance of infaunal bioturbators for the functioning marine ecosystems cannot be overstated. Inhabitants estuarine and coastal habitats are expected to show resilience fluctuations in seawater temperature pH, which adds complexity our understanding effects global change drivers. Further, stress responses may propagated through chemical cues within across species, amplify costs life alter species interactions. Research into molecular mechanisms underlying this has been limited by a lack annotated genomes associated tools. In study, we present first chromosome-level, draft genome ragworm Hediste diversicolor , specifically mapping genes important communication, sensing pH homeostasis. Using these resources, then evaluate transcriptomic behavioural two distinct populations — one field-sampled from Portugal (Ria Formosa) laboratory-acclimated -bred United Kingdom (Humber) changes temperature, odour low pH-stressed predator. Both displayed adaptive future oceanic conditions, with targeted acid-base regulation Ria Formosa population experiment, broader metabolism growth Humber experiment. Chemical stressed fish predators induced related Schreckstoff biosynthesis ragworms. Additionally, under ocean conditions including increased exhibited signs cellular damage. Our findings using new offer novel insights arsenal aids predicting impacts an increasingly acidified unstable ocean, transfer knowledge investigate less tolerance.

Language: Английский

Citations

0
De novo genome assemblies of two cryptodiran turtles with ZZ/ZW and XX/XY sex chromosomes provide insights into patterns of genome reshuffling and uncover novel 3D genome folding in amniotes DOI Creative Commons
Basanta Bista, Laura González-Rodelas, Lucía Álvarez-González

et al.

Genome Research, Journal Year: 2024, Volume and Issue: 34(10), P. 1553 - 1569

Published: Oct. 1, 2024

Understanding the evolution of chromatin conformation among species is fundamental to elucidate architecture and plasticity genomes. Nonrandom interactions linearly distant loci regulate gene function in species-specific patterns, affecting genome function, evolution, and, ultimately, speciation. Yet, data from nonmodel organisms are scarce. To capture macroevolutionary diversity vertebrate conformation, here we generate de novo assemblies for two cryptodiran (hidden-neck) turtles via Illumina sequencing, chromosome capture, RNA-seq:

Language: Английский

Citations

0