The Phyllosphere of Nigerian Medicinal Plants Euphorbia lateriflora and Ficus thonningii is inhabited by a specific Microbiota DOI Creative Commons
Anderson O. Oaikhena, Morenike E. Coker,

Dorothy Cyril‐Okoh

et al.

Research Square (Research Square), Journal Year: 2023, Volume and Issue: unknown

Published: Nov. 14, 2023

Abstract Background Medicinal plant microbiota is highly specific and can contribute to medicinal activity. However, the majority of species have not yet been studied. Here, we investigated phyllosphere composition two common Nigerian plants, Euphorbia lateriflora Ficus thonningii , by a polyphasic approach combining analyses metagenomic DNA isolates. Results Microbial abundance estimated via qPCR using marker gene primers showed that all leaf samples were densely colonized with up 10 8 per gram higher bacterial fungal than Archaea. While no statistically significant differences between both found for abundance, amplicon sequencing 16S rRNA ITS genes revealed distinct composition, only seven 27 genera isolated represented on plants. We observed dominance Sphingomonas spp. members Xanthomonadaceae Enterobacteriaceae also in high numbers. The most dominant families plants Cladosporiaceae, Mycosphaerellaceae Trichosphaeriaceae . In addition, 225 plant-specific isolates identified, Pseudomonadota being dominant. Interestingly, 29 are likely previously unknown, 14 these belong Burkholderiales proportion, 56% 40% from E. F. respectively, characterized as various Escherichia coli growth was influenced extractable secondary metabolites Conclusions Our results suggest diverse microbial community inhabits leaves including potentially new producers antimicrobials.

Language: Английский

Increased antibiotic resistance gene abundance linked to intensive bacterial competition in the phyllosphere across an elevational gradient DOI Creative Commons
Yihui Ding, Ruijun Ma, Ran Zhang

et al.

Environmental Microbiology Reports, Journal Year: 2024, Volume and Issue: 16(6)

Published: Nov. 21, 2024

Abstract Antibiotic resistance genes (ARGs) are ancient and widespread in natural habitats, providing survival advantages for microbiomes under challenging conditions. In mountain ecosystems, phyllosphere bacterial communities face multiple stress conditions, the elevational gradients of mountains represent crucial environmental studying biodiversity distribution patterns. However, patterns ARGs along gradients, their correlation with community structures, remain poorly understood. Here, we applied metagenomic analyses to investigate abundance diversity 88 samples collected from Mount Tianmu, a national reserve. Our results showed that increased was dominated by multidrug efflux pumps. The composition communities, rather than plant traits or abiotic factors, significantly affected ARG abundance. Moreover, correlated greater phylogenetic overdispersion proportion negative associations co‐occurrence networks, suggesting competition primarily shapes resistomes. These findings constitute major advance resistomes elevations, emphasizing significant impact structure assembly on distribution, essential understanding emergence ARGs.

Language: Английский

Citations

1
Seed synthetic community matters and its impact on seedling is strain- and not species-dependant DOI Creative Commons
Sébastien Massart

Peer Community In Microbiology, Journal Year: 2023, Volume and Issue: unknown

Published: Sept. 20, 2023

A recommendation of: Marie Simonin, Anne Preveaux, Coralie Marais, Tiffany Garin, Gontran Arnault, Alain Sarniguet, Matthieu Barret Transmission of synthetic seed bacterial communities to radish seedlings: impact on microbiota assembly and plant phenotype https://doi.org/10.1101/2023.02.14.527860

Language: Английский

Citations

2
Analysis of microbial dynamics in the soybean root-associated environments from community to single-cell levels DOI
Masako Kifushi, Yohei Nishikawa, Masahito Hosokawa

et al.

Journal of Bioscience and Bioengineering, Journal Year: 2024, Volume and Issue: 137(6), P. 429 - 436

Published: April 4, 2024

Language: Английский

Citations

0
Mediator kinase module proteins, genetic alterations and expression of super-enhancer regulated genes in colorectal cancer DOI
Ioannis A. Voutsadakis

Pharmacological Reports, Journal Year: 2024, Volume and Issue: 76(3), P. 535 - 556

Published: April 11, 2024

Language: Английский

Citations

0
Field conditions greatly modify a major growth-defense tradeoff inArabidopsis thaliana DOI Creative Commons
Derek S. Lundberg, Sonja Kersten, Ezgi Mehmetoğlu

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 15, 2024

Abstract When plants defend themselves from pathogens, this often comes with a trade-off: the same genes that protect plant disease can also reduce its growth and fecundity in absence of pathogens. One protein implicated major growth-defense trade-off Arabidopsis thaliana is ACCELERATED CELL DEATH 6 (ACD6), an ion channel modulates salicylic acid (SA) synthesis to potentiate wide range defenses. Wild populations maintain significant functional variation ACD6 gene, some alleles making hyperactive. In greenhouse, hyperactive are resistant diverse yet smaller stature, their leaves senesce earlier, they set fewer seeds. We hypothesized such would not only affect microbial but more generally leaf microbiome assembly wild. To test this, we grew hyperactive, standard, defective field-collected soil, both climate-controlled conditions outdoors. surveyed visual phenotypes, gene expression, hormone levels, seed production, each environment. our surprise, discovered mature field plants, stark contrast greenhouse were unaffected by genotype, suggesting additional abiotic and/or signals present outdoors – greatly modulate activity.

Language: Английский

Citations

0
The phyllosphere of Nigerian medicinal plants, Euphorbia lateriflora and Ficus thonningii is inhabited by a specific microbiota DOI Creative Commons
Anderson O. Oaikhena, Morenike E. Coker,

Dorothy Cyril‐Okoh

et al.

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: Oct. 1, 2024

Language: Английский

Citations

0
Biotic interactions shape infection outcomes inArabidopsis DOI Creative Commons
Maryam Mahmoudi, Juliana Almario, Yiheng Hu

et al.

Published: Oct. 26, 2024

Abstract The plant microbiome protects plants from stresses, including pathogen attacks. However, identifying microbes that provide protection remains challenging in complex microbial communities. In this study, we analysed samples natural A. thaliana populations, both infected with the pathogenic oomycete Albugo laibachii and uninfected plants, over six years. Using machine learning classification models, achieved high accuracy distinguishing based on abundance. We identified 80 key taxa associated health disease. Among health-associated (HCom), selected bacteria, fungi, cercozoa effectively reduced presence co-inoculation assays. comparison, disease-associated (DCom) were less effective conferring protection. Our findings highlight complexity of plant-microbe interactions advance our understanding roles disease ecology. By integrating ecological insights learning, take a significant step towards designing robust consortia enhance resilience against pathogens.

Language: Английский

Citations

0
Impact of timing on the invasion of synthetic bacterial communities DOI Creative Commons
Keven Dooley, Lucas P. Henry, Joy Bergelson

et al.

The ISME Journal, Journal Year: 2024, Volume and Issue: 18(1)

Published: Jan. 1, 2024

Language: Английский

Citations

0
Bacterial community complexity in the phyllosphere penalises specialists over generalists DOI Creative Commons
R. Schlechter, Mitja N. P. Remus‐Emsermann

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Nov. 9, 2023

Abstract The leaf surface, i.e. the phylloplane, is an oligotrophic and heterogeneous environment due to its topography uneven distribution of resources. Despite being a limiting environment, leaves host bacteria that are abundant establish spatially-structured communities. However, factors drive spatial patterns not well understood. Since leaf-associated can have beneficial effects their host, understanding rules community assembly lead novel strategies for crop protection. To investigate changes in population density synthetic communities, we examined behaviour two prevalent bacterial groups Arabidopsis thaliana microbiota: Methylobacterium spp. (specialists) Sphingomonas (generalists). We designed communities composed (S2) or three strains (S3) full factorial design tested whether structure S3 be explained by pairwise comparisons S2. Our results showed specialists more susceptible densities than generalists, with lower aggregation when specialist Additionally, were sufficient explain observed S3, suggesting higher-order interactions play role resulting complex at micrometre scale.

Language: Английский

Citations

1
Transmission of synthetic seed bacterial communities to radish seedlings: impact on microbiota assembly and plant phenotype DOI Creative Commons
Marie Simonin,

Anne Préveaux,

Coralie Marais

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Feb. 14, 2023

ABSTRACT Seed-borne microorganisms can be pioneer taxa during germination and seedling emergence. Still, the identity phenotypic effects of these that constitute a primary inoculum plant microbiota is mostly unknown. Here, we studied transmission bacteria from radish seeds to seedlings using inoculation individual seed-borne strains synthetic communities (SynComs) under in vitro conditions. The SynComs were composed highly abundant prevalent, sub-dominant or rare bacterial seed taxa. We monitored each strain alone gyrB gene amplicon sequencing assessed their impacts on phenotype. All successfully colonized able reconstruct richness gradient (6, 8 12 strains) both seedlings. Stenotrophomonas rhizophila became dominant three but most had variable success (i.e increasing, stable decreasing transition) also depended SynCom richness. Most no effect phenotypes, at exception Pseudomonas viridiflava Paenibacillus sp. detrimental development. Abnormal morphologies observed with proportions decreased highest level. Interestingly, some previously identified as core ( viridiflava, Erwinia persicina) associated phenotypes either isolation SynComs. These results confirm microbiome includes pathogenic not only commensal mutualistic Altogether, show effectively manipulate diversity thus represents promising tool better understand early stages assembly. This study highlights strong differences between native colonization survival habitats.

Language: Английский

Citations

0