Antimicrobial resistance level and conjugation permissiveness shape plasmid distribution in clinical enterobacteria DOI Creative Commons
Aída Alonso-del Valle, Laura Toribio-Celestino, Anna Quirant

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: April 3, 2023

Abstract Conjugative plasmids play a key role in the dissemination of antimicrobial resistance (AMR) genes across bacterial pathogens. AMR are widespread clinical settings, but their distribution is not random, and certain associations between clones particularly successful. For example, globally spread carbapenem plasmid pOXA-48 can use wide range enterobacterial species as hosts, it usually associated with small number specific Klebsiella pneumoniae clones. These successful represent an important threat for hospitalized patients. However, knowledge remains limited about factors determining clinically relevant bacteria. Here, we combined vitro vivo experimental approaches to analyze pOXA-48-associated levels conjugation dynamics collection wild type strains isolated from Our results reveal significant variability these traits different spp. showing higher pOXA-48-mediated frequencies than Escherichia coli strains. Using experimentally determined parameters, developed simple mathematical model interrogate contribution permissiveness communities. The simulations revealed that subset clones, combining high permissiveness, critical stabilizing polyclonal microbial help explain preferential association K. settings. More generally, our study reveals species- strain-specific plasmid-associated phenotypes shape evolution Significance statement disseminate Understanding rules governing communities therefore crucial controlling global crisis. In this study, analyzed great relevance, pOXA-48, bacteria recovered We reported degree two phenotypes, level ability, based on results, studied how arise accounting understand

Language: Английский

Inhibition of pathogenic bacterial carbonic anhydrases by monothiocarbamates DOI Creative Commons
Simone Giovannuzzi, Anil Kumar Marapaka, Nader S. Abutaleb

et al.

Journal of Enzyme Inhibition and Medicinal Chemistry, Journal Year: 2023, Volume and Issue: 38(1)

Published: Nov. 23, 2023

Carbonic anhydrases (CAs) from the pathogenic bacteria Nesseria gonorrhoeae and vancomycin-resistant enterococci (VRE) have recently been validated as antibacterial drug targets. Here we explored inhibition of α-CA N. (α-NgCA), α- γ-class enzymes Enterococcus faecium (α-EfCA γ-EfCA) with a panel aliphatic, heterocyclic aryl-alkyl primary/secondary monothiocarbamates (MTCs). α-NgCA was inhibited in vitro KIs ranging 0.367 to 0.919 µM. The compounds α-EfCA γ-EfCA KI ranges 0.195–0.959 µM 0.149–1.90 µM, respectively. Some MTCs were also investigated for their inhibitory effects on growth clinically-relevant VRE strains. No noted all MTCs, whereas one compound (13) strains at concentrations 16 64 µg/mL. This suggests that 13 may be potential agent against gonorrhoeae.

Language: Английский

Citations

10
Global dissemination of Klebsiella pneumoniae in surface waters: genomic insights into drug resistance, virulence, and clinical relevance DOI Creative Commons
Damian Rolbiecki, Edyta Kiedrzyńska, Małgorzata Czatzkowska

et al.

Drug Resistance Updates, Journal Year: 2025, Volume and Issue: 79, P. 101204 - 101204

Published: Jan. 21, 2025

Language: Английский

Citations

0
Metagenomic analysis of pristine oil sheds new light on the global distribution of microbial genetic repertoire in hydrocarbon-associated ecosystems DOI Creative Commons
Julia Plewka,

Armando Alibrandi,

Till L. V. Bornemann

et al.

microLife, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 23, 2025

Abstract Oil reservoirs are society's primary source of hydrocarbons. While microbial communities in industrially exploited oil have been investigated the past, pristine untapped little explored, as distribution patterns respective genetic signatures. Here, we show that a sample contains complex community consisting bacteria and fungi for degradation We identified microorganisms their pathways methane, n-alkanes, mono-aromatic, polycyclic aromatic compounds metagenome retrieved from biodegraded petroleum encountered subsurface reservoir Barents Sea. Capitalizing on marker genes metagenomes public data mining, compared prokaryotes, putative viruses, plasmids sampled site to those ten other hydrocarbon-associated sites, revealing shared network species elements across globe. To test potential dispersal microbes predicted via seawater, our findings Tara Ocean dataset, resulting broad prokaryotic viral Although frequently between plasmids, however, showed coverage Oceans suggesting an undiscovered mode transfer hydrocarbon-affected ecosystems. Based analyses, information is globally propose currents physical occurrences within ocean along with deep aquifers major distributors prokaryotes viruses into these

Language: Английский

Citations

0
Plasmid-driven strategies for clone success in Escherichia coli DOI Creative Commons
Sergio Arredondo-Alonso, Anna K. Pöntinen, João Alves Gama

et al.

Nature Communications, Journal Year: 2025, Volume and Issue: 16(1)

Published: April 3, 2025

Language: Английский

Citations

0
Plasmid–bacteria associations in the clinical context DOI
Laura Toribio-Celestino, Álvaro San Millán

Trends in Microbiology, Journal Year: 2025, Volume and Issue: unknown

Published: May 1, 2025

Language: Английский

Citations

0
Challenges for developing bacterial CA inhibitors as novel antibiotics DOI
Claudiu T. Supuran

˜The œEnzymes, Journal Year: 2024, Volume and Issue: unknown, P. 383 - 411

Published: Jan. 1, 2024

Language: Английский

Citations

3
Global epistasis in plasmid-mediated antimicrobial resistance DOI Creative Commons
Javier DelaFuente, Juan Díaz‐Colunga,

Álvaro Sánchez

et al.

Molecular Systems Biology, Journal Year: 2024, Volume and Issue: 20(4), P. 311 - 320

Published: Feb. 26, 2024

Abstract Antimicrobial resistance (AMR) in bacteria is a major public health threat and conjugative plasmids play key role the dissemination of AMR genes among bacterial pathogens. Interestingly, association between pathogens not random certain associations spread successfully at global scale. The burst genome sequencing has increased resolution epidemiological programs, broadening our understanding plasmid distribution populations. Despite immense value these studies, ability to predict future plasmid-bacteria remains limited. Numerous empirical studies have recently reported systematic patterns genetic interactions that enable predictability, phenomenon known as epistasis. In this perspective, we argue epistasis hold potential genomes, thereby facilitating prediction successful associations. To assess validity idea, use previously published data identify clinically relevant Furthermore, using simple mechanistic models antibiotic resistance, illustrate how may allow us generate new hypotheses on mechanisms associated with Collectively, aim illustrating relevance exploring context biology.

Language: Английский

Citations

2
Landscape of plasmids encoding β-lactamases in disinfection residual Enterobacteriaceae from wastewater treatment plants DOI
Zejun Lin, Zhenchao Zhou,

Xinyi Shuai

et al.

Water Research, Journal Year: 2024, Volume and Issue: 255, P. 121549 - 121549

Published: March 29, 2024

Language: Английский

Citations

2
Costs of antibiotic resistance genes depend on host strain and environment and can influence community composition DOI Creative Commons

Huei-Yi Lai,

Tim F. Cooper

Proceedings of the Royal Society B Biological Sciences, Journal Year: 2024, Volume and Issue: 291(2025)

Published: June 1, 2024

Antibiotic resistance genes (ARGs) benefit host bacteria in environments containing corresponding antibiotics, but it is less clear how they are maintained where antibiotic selection weak or sporadic. In particular, few studies have measured if the direct effect of ARGs on fitness fixed depends strain, perhaps marking some ARG–host combinations as selective refuges that can maintain absence selection. We quantified effects six 11 diverse Escherichia spp. strains. Three (bla TEM-116 , cat and dfrA5 encoding to β-lactams, chloramphenicol, trimethoprim, respectively) imposed an overall cost, all had at least one reflecting a significant strain interaction effect. A simulation predicts these interactions cause success depend available strains, and, lesser extent, present community. These results indicate importance considering ARG across different especially potential refuge strains allow persist selection, efforts understand dynamics.

Language: Английский

Citations

2
Compensatory evolution of chromosomes and plasmids counteracts the plasmid fitness cost DOI Creative Commons
Ziyi Liu,

Qiuyun Zhao,

He Zhang

et al.

Ecology and Evolution, Journal Year: 2024, Volume and Issue: 14(8)

Published: Aug. 1, 2024

Abstract Plasmids incur a fitness cost that has the potential to restrict dissemination of resistance in bacterial pathogens. However, bacteria can overcome this disadvantage by compensatory evolution maintain their resistance. Compensatory occur via both chromosomes and plasmids, but there are few reviews regarding topic, most them focus on plasmids. In review, we provide comprehensive overview currently reported mechanisms underlying elucidate key targets regulating plasmid cost, discuss future challenges field. We found primarily arises from mutations transcriptional regulatory factors, whereas plasmids predominantly involves three pathways: copy number regulation, conjugation transfer efficiency, expression antimicrobial (AMR) genes. Furthermore, importance reasonable selection research subjects effective integration diverse advanced methods is also emphasized our study mechanisms. Overall, review establishes theoretical framework aims innovative ideas for minimizing emergence spread AMR

Language: Английский

Citations

2