Pseudomonas aeruginosa surface motility and invasion into competing communities enhance interspecies antagonism DOI Creative Commons
Andrea Sánchez Peña, James B. Winans, Carey D. Nadell

et al.

mBio, Journal Year: 2024, Volume and Issue: 15(9)

Published: Aug. 6, 2024

ABSTRACT Chronic polymicrobial infections involving Pseudomonas aeruginosa and Staphylococcus aureus are prevalent, difficult to eradicate, associated with poor health outcomes. Therefore, understanding interactions between these pathogens is important inform improved treatment development. We previously demonstrated that P. attracted S. using type IV pili (TFP)-mediated chemotaxis, but the impact of attraction on growth physiology remained unknown. Using live single-cell confocal imaging visualize microcolony structure, spatial organization, survival during coculture, we found interspecies chemotaxis provides a competitive advantage by promoting invasion into disruption microcolonies. This behavior renders susceptible antimicrobials. Conversely, in absence TFP motility, cells exhibit reduced colonies. Instead, builds cellular barrier adjacent secretes diffusible, bacteriostatic antimicrobials like 2-heptyl-4-hydroxyquinoline- N -oxide (HQNO) Reduced leads formation denser thicker colonies increased HQNO-mediated lactic acid fermentation, physiological change could complicate strategies. Finally, show motility modifications structure enhance competition against . Overall, studies expand our how TFP-mediated facilitates interactions, highlighting importance positioning mixed-species communities. IMPORTANCE The nature many chronic makes their eradication challenging. Particularly, coisolation from airways people cystic fibrosis wound common severe clinical complex interplay not fully understood, need for continued research improve management infections. Our study unveils , invades neighboring colonies, anti-staphylococcal factors interior colony. Upon inhibition thus invasion, colony architecture changes dramatically, whereby protected antagonism responds through alterations may further hamper treatment. These reinforce accumulating evidence structuring can dictate community resilience reveal critical drivers competition.

Language: Английский

3D-printed wound dressings containing a fosmidomycin-derivative prevent Acinetobacter baumannii biofilm formation DOI Creative Commons
Frits van Charante, David Martínez-Pérez, Clara Guarch-Pérez

et al.

iScience, Journal Year: 2023, Volume and Issue: 26(9), P. 107557 - 107557

Published: Aug. 7, 2023

Acinetobacter baumannii causes a wide range of infections, including wound infections. Multidrug-resistant A. is major healthcare concern and the development novel treatments against these infections needed. Fosmidomycin repurposed antimalarial drug targeting non-mevalonate pathway, several derivatives show activity toward baumannii. We evaluated antimicrobial CC366, fosmidomycin prodrug, collection strains, using various in vitro vivo models; emphasis was placed on evaluation its anti-biofilm activity. also developed 3D-printed dressing containing melt electrowriting technology. Minimal inhibitory concentrations CC366 ranged from 1 to 64 μg/mL, showed good biofilm moderate eradicating vitro. successfully eluted dressing, dressings prevented formation baumannnii biofilms reduced infection an mouse model.

Language: Английский

Citations

3
Metabolic and transcriptional activities underlie stationary-phase Pseudomonas aeruginosa sensitivity to Levofloxacin DOI Creative Commons
Patricia J. Hare,

Juliet R. Gonzalez,

Ryan M. Quelle

et al.

Microbiology Spectrum, Journal Year: 2023, Volume and Issue: 12(1)

Published: Dec. 11, 2023

ABSTRACT The ubiquitous opportunistic pathogen Pseudomonas aeruginosa is highly adaptive and refractory to several different classes of antibiotics. However, we found in this study that stationary-phase P. cultures exhibit greater sensitivity the fluoroquinolone Levofloxacin (Levo) than other bactericidal antibiotics, including an aminoglycoside (Tobramycin) β-lactam (Aztreonam). To understand basis sensitivity, conducted time-lapse fluorescence microscopy experiments cells during Levo treatment. We discovered die rapidly treatment undergo heterogeneous morphological changes, explosive lysis, filamentation, gradual loss membrane integrity as evidenced by propidium iodide uptake. These morphologies are reminiscent how model organism Escherichia coli appears when recovering from treatment, a period activation DNA damage-induced SOS response crucial. Accordingly, monitored survival Δ recA mutants not involved like it for E. . hypothesized may be due maintaining active metabolism stationary phase. determined transcribe, maintain reductase activity, accumulate reactive metabolic species which contribute Levo-mediated death. By elucidating sustain activity phase, can design strategies sensitize these persistent subpopulations efficacy clinically important antibiotic. IMPORTANCE bacterial responsible variety chronic human infections. Even absence identifiable resistance mutations, tolerate lethal antibiotic doses through phenotypic biofilm formation quiescence. In study, maintains phase compared organism, , has traditionally been used tolerance. demonstrate hallmarks tolerance conserved timing cell death necessity damage survival. heightened fluoroquinolones attributed maintained transcriptional activity. Our data suggest perturbations suppress transcription respiration actually protect against class

Language: Английский

Citations

2
Metabolomic Analysis of Polymicrobial Wound Infections and an Associated Adhesive Bandage DOI

Monica Ness,

Avery L. Holmes, Chaoyi Wu

et al.

Journal of the American Society for Mass Spectrometry, Journal Year: 2023, Volume and Issue: 34(9), P. 1847 - 1857

Published: June 8, 2023

Concerns about ion suppression, spectral contamination, or interference have led to avoidance of polymers in mass spectrometry (MS)-based metabolomics. This avoidance, however, has left many biochemical fields underexplored, including wounds, which are often treated with adhesive bandages. Here, we found that despite previous concerns, the addition an bandage can still result biologically informative MS data. Initially, a test LC-MS analysis was performed on mixture known chemical standards and polymer extract. Results demonstrated successful removal polymer-associated features through data processing step. Furthermore, presence did not interfere metabolite annotation. method then implemented context murine surgical wound infections covered inoculated

Language: Английский

Citations

1
The past, present and future of polymicrobial infection research: Modelling, eavesdropping, terraforming and other stories DOI

Éva Bernadett Bényei,

Rahan Rudland Nazeer,

Isabel Askenasy

et al.

Advances in microbial physiology/Advances in Microbial Physiology, Journal Year: 2024, Volume and Issue: unknown, P. 259 - 323

Published: Jan. 1, 2024

Language: Английский

Citations

0
Pseudomonas aeruginosa surface motility and invasion into competing communities enhance interspecies antagonism DOI Creative Commons
Andrea Sánchez Peña, James B. Winans, Carey D. Nadell

et al.

mBio, Journal Year: 2024, Volume and Issue: 15(9)

Published: Aug. 6, 2024

ABSTRACT Chronic polymicrobial infections involving Pseudomonas aeruginosa and Staphylococcus aureus are prevalent, difficult to eradicate, associated with poor health outcomes. Therefore, understanding interactions between these pathogens is important inform improved treatment development. We previously demonstrated that P. attracted S. using type IV pili (TFP)-mediated chemotaxis, but the impact of attraction on growth physiology remained unknown. Using live single-cell confocal imaging visualize microcolony structure, spatial organization, survival during coculture, we found interspecies chemotaxis provides a competitive advantage by promoting invasion into disruption microcolonies. This behavior renders susceptible antimicrobials. Conversely, in absence TFP motility, cells exhibit reduced colonies. Instead, builds cellular barrier adjacent secretes diffusible, bacteriostatic antimicrobials like 2-heptyl-4-hydroxyquinoline- N -oxide (HQNO) Reduced leads formation denser thicker colonies increased HQNO-mediated lactic acid fermentation, physiological change could complicate strategies. Finally, show motility modifications structure enhance competition against . Overall, studies expand our how TFP-mediated facilitates interactions, highlighting importance positioning mixed-species communities. IMPORTANCE The nature many chronic makes their eradication challenging. Particularly, coisolation from airways people cystic fibrosis wound common severe clinical complex interplay not fully understood, need for continued research improve management infections. Our study unveils , invades neighboring colonies, anti-staphylococcal factors interior colony. Upon inhibition thus invasion, colony architecture changes dramatically, whereby protected antagonism responds through alterations may further hamper treatment. These reinforce accumulating evidence structuring can dictate community resilience reveal critical drivers competition.

Language: Английский

Citations

0