Two-Photon and Multiphoton Microscopy in Anterior Segment Diseases of the Eye DOI Open Access
Merrelynn Hong, Shu Zhen Chong,

Yun Yao Goh

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(3), P. 1670 - 1670

Published: Jan. 30, 2024

Two-photon excitation microscopy (TPM) and multiphoton fluorescence (MPM) are advanced forms of intravital high-resolution functional techniques that allow for the imaging dynamic molecular processes resolve features biological tissues interest. Due to cornea's optical properties uniquely accessible position globe, it is possible image cells longitudinally investigate ocular surface physiology disease. MPM can also be used in vitro investigation drug kinetics tissues. In corneal immunology, performed via use TPM, thought intraepithelial dendritic found resemble tissue-resident memory T cells, reporter mice with labeled plasmacytoid imaged understand protective antiviral defenses eye. limbal progenitor by reporters, localization epithelial replenishment evaluated advance stem cell biology. studies conjunctiva sclera, such together second harmonic generation allows delineation matrix wound healing, especially following glaucoma surgery. conclusion, these models play a pivotal role progress science translational research.

Language: Английский

Biologicals and Biomaterials for Corneal Regeneration and Vision Restoration in Limbal Stem Cell Deficiency DOI Creative Commons
Nick Di Girolamo

Advanced Materials, Journal Year: 2024, Volume and Issue: unknown

Published: May 23, 2024

The mammalian cornea is decorated with stem cells bestowed the life-long task of renewing epithelium, provided they remain healthy, functional, and in sufficient numbers. If not, a debilitating disease known as limbal cell deficiency (LSCD) can develop causing blindness. Decades after first (SC) therapy devised to treat this condition, patients continue suffer unacceptable failures. During time, improvements therapeutics have included identifying better markers isolate robust SC populations nurturing them on crudely modified biological or biomaterial scaffolds including human amniotic membrane, fibrin, contact lenses, prior their delivery. Researchers are now gathering information about biomolecular biomechanical properties corneal niche decipher what and/or synthetic materials be incorporated into these carriers. Advances biomedical engineering electrospinning 3D bioprinting surface functionalization micropatterning, self-assembly models, generated wealth biocompatible, biodegradable, integrating choose from, some which being tested for delivery capacity hope improving clinical outcomes LSCD.

Language: Английский

Citations

10
Redefining our vision: an updated guide to the ocular immune system DOI
Mengliang Wu, Erica L. Fletcher, Holly R. Chinnery

et al.

Nature reviews. Immunology, Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 30, 2024

Language: Английский

Citations

9
Persistence of Contact Lens-Induced Corneal Parainflammation Following Lens Removal DOI Creative Commons

Ananya Datta,

Ji Hyun Lee, Tiffany Truong

et al.

Investigative Ophthalmology & Visual Science, Journal Year: 2024, Volume and Issue: 65(3), P. 8 - 8

Published: March 11, 2024

Purpose: Contact lens wear induces corneal parainflammation involving increased immune cell numbers after 24 hours' (CD11c+, Lyz2+, γδ-T cells) and six days' (Ly6G+ wear. We investigated the time course of onset resolution these responses. Methods: LysMcre or C57BL/6J mice were fitted with a contact (four to 48 hours). Contralateral eyes did not lenses. After removal, MHC-II+ Ly6G+ cells examined by quantitative imaging. RT-qPCR determined cytokine gene expression. Results: Lens for hours Lyz2+ versus contralateral approximately two-fold. Corneas remained free visible pathology. The response was observed four 12 wear, nor plus no removal further (∼48% one day), which persisted days, returning baseline seven days. in at baseline. showed similar but without increasing removal. reduced day discontinuation, correlating IL-1β IL-18 induce responses days Conclusions: Lens-induced requires persists requiring reversal. may suppress initial significance during remains be determined.

Language: Английский

Citations

4
Spatial Transcriptomics and Single-Cell RNA-Sequencing Revealed Dendritic Cell-Mediated Inflammation in Keratoconus DOI
Shiqi Luo, Jingying Li, Yan Yang

et al.

The Ocular Surface, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Language: Английский

Citations

0
Shared Phenotypes of Immune Cells Recruited to the Cornea and the Surface of the Lens in Response to Formation of Corneal Erosions DOI
Phuong M. Le,

Sonali Pal‐Ghosh,

Mary Ann Stepp

et al.

American Journal Of Pathology, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Language: Английский

Citations

0
Does orthokeratology contact lens wear suppress the immune cell response in the human corneal epithelium, while soft contact lens wear enhances it? DOI Creative Commons

Rabia Mobeen,

Fiona Stapleton, Cecilia Chao

et al.

Contact Lens and Anterior Eye, Journal Year: 2025, Volume and Issue: unknown, P. 102380 - 102380

Published: Jan. 1, 2025

Orthokeratology (OK) contact lenses are increasingly prescribed for myopia control but their impact on corneal epithelial immune cells (CEIC) is unclear. This study compares CEIC in OK wearers to soft lens (SCL) and non-wearers. In vivo confocal microscope images at the central mid-peripheral subbasal level were evaluated 18 wearers, SCL non-wearers (mean age 27.6±8.0 years; 65% female). Corneal cell density was manually quantified morphology graded using a published system. Statistical significances (p<0.05) examined generalised estimating equations. lower than (p=0.03) cornea, not mid-periphery. Compared had smaller bodies, with fewer participants displaying dendrites, long thick dendrites both locations (p<0.001). also bodies (p=0.01) locations. Contrarily, larger greater proportion of compared (p≤0.04) higher cornea mid-periphery (p<0.001) (p=0.01), (p=0.26). long-term wear, observed epithelium number less likely present suggesting suppressed response wear which should be investigated further.

Language: Английский

Citations

0
Contact Lens Wear Alters Transcriptional Responses to Pseudomonas aeruginosa in Both the Corneal Epithelium and the Bacteria DOI Creative Commons
Naren Gajenthra Kumar, Melinda R. Grosser, Stephanie Wan

et al.

Investigative Ophthalmology & Visual Science, Journal Year: 2025, Volume and Issue: 66(2), P. 31 - 31

Published: Feb. 11, 2025

Purpose: Healthy corneas resist colonization by virtually all microbes, yet contact lens wear can predispose the cornea to sight-threatening infection with Pseudomonas aeruginosa. Here, we explored how changes corneal epithelium transcriptional responses P. aeruginosa and its impact on bacterial gene expression. Methods: Male female C57BL/6J mice were fitted a one eye for 24 hours. After removal, immediately challenged 4 hours A separate group of naïve was similarly bacteria. Bacteria-challenged eyes compared uninoculated controls, as alone. Total RNA sequencing determined Results: Prior profoundly altered response aeruginosa, including upregulated pattern recognition receptors (tlr3, nod1); downregulated lectin pathway complement activation (masp1); amplified upregulation tcf7, gpr55, ifi205, wfdc2 (immune defense); further suppression efemp1 (corneal stromal integrity). Without wear, mitochondrial ubiquinone metabolism genes. Lens alone axl, grn, gpr55 defense) Ca2+-dependent genes necab1, snx31, npr3. exposure prior wearing versus virulence (popD), regulation (rsmY, PA1226), antimicrobial resistance (arnB, oprR). Conclusions: impacts expression, altering responds it favoring virulence, survival, adaptation. Impacted associated networks provide avenues research better understand pathogenesis.

Language: Английский

Citations

0
Cecelia: a multifunctional image analysis toolbox for decoding spatial cellular interactions and behaviour DOI Creative Commons
Dominik Schienstock, Jyh Liang Hor, Sapna Devi

et al.

Nature Communications, Journal Year: 2025, Volume and Issue: 16(1)

Published: Feb. 24, 2025

Abstract With the ever-increasing complexity of microscopy modalities, it is imperative to have computational workflows that enable researchers process and perform in-depth quantitative analysis resulting images. However, allow flexible, interactive intuitive from raw images analysed data are lacking for many experimental use-cases. Notably, integrated software solutions complex 3D live cell sorely needed. To address this, we present Cecelia, a toolbox integrates various open-source packages into coherent management suite make multidimensional image accessible non-specialists. We describe application Cecelia several immunologically relevant scenarios development an unbiased approach distinguish dynamic behaviours imaging data. available as package with Shiny app interface ( https://github.com/schienstockd/cecelia ). envision this framework its approaches will be broad use biological researchers.

Language: Английский

Citations

0
Decoding the corneal immune microenvironment in healthy and diabetic mice during corneal wound healing DOI
Yujing Lin, Xiaowen Zhang, Di Sun

et al.

The Ocular Surface, Journal Year: 2025, Volume and Issue: 37, P. 68 - 79

Published: Feb. 27, 2025

Language: Английский

Citations

0
Metformin therapy modifies corneal neuroimmune abnormalities in people with type 2 diabetes DOI Creative Commons

Amy T. Tsoi,

J. Sng,

Shyam Sunder Tummanapalli

et al.

Diabetologia, Journal Year: 2025, Volume and Issue: unknown

Published: March 7, 2025

Diabetic peripheral neuropathy is a debilitating microvascular complication of diabetes mellitus, with limited disease-modifying therapies to date. This study aimed assess the effect metformin on corneal sub-basal nerve plexus as outcome measure in people type 2 diabetes. A cohort 36 participants receiving therapy were recruited and underwent clinical assessment, confocal microscopy conduction studies. Concurrently, not selected disease controls matched for age, sex, duration, BMI, eGFR, HbA1c, use other oral glucose-lowering agents used treatment metabolic syndrome. Additionally, 25 healthy control assessed age sex. Medical record data over previous 20 years analysed prior current all Participants had higher fibre density (p=0.020), length (p=0.020) fractal dimension (p=0.003) compared those therapy. The inferior whorl dendritic cell was significantly lower group non-metformin (p=0.043). Metformin associated superior parameters neuroimmune tone plexus. provides further evidence that may be neuroprotective diabetic neuropathy.

Language: Английский

Citations

0