Genome-Wide Identification, Expression, and Interaction Analysis of the Auxin Response Factor and AUX/IAA Gene Families in Vaccinium bracteatum

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(15), P. 8385 - 8385

Published: Aug. 1, 2024

Auxin, a plant hormone, plays diverse roles in the modulation of growth and development. The transport signal transduction auxin are regulated by various factors involved shaping morphology responding to external environmental conditions. is primarily governed following two gene families: response factor (

Language: Английский

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Genome-Wide Identification of the ARF Gene Family in Three Dendrobium Species and Its Expression Pattern Analysis in D. nobile Flower

Horticulturae, Journal Year: 2024, Volume and Issue: 10(6), P. 568 - 568

Published: May 29, 2024

The ARF gene family is a representative transcription factor that plays crucial role in the regulation of various growth and development processes plants. Although has been identified five Orchidaceae species, limited research conducted on within Dendrobium. To explore genes different Dendrobium we selected chromosome-level genomic data from D. nobile, chrysotoxum, huoshanense for genome-wide identification, to analyze expression patterns nobile flower. In this study, 13, 18, 23 were genomes huoshanense, respectively. These then subsequently classified into four classes (Classes I, II, III, IV) based our phylogenetic analysis. Additional protein sequence analysis found 30 proteins with three classically conserved structural domains (BDB, MR, RD) present species. Our structure comparative also same evolutionary branch similar intron-exon features. Specifically, Class I III display longer introns potentially constitute distinctive characteristic Gene showed potential involvement DnoARF 5, 7, 10, 11, 12, 16, 17, initial differentiation flower bud formation. Likewise, transcriptomic RT-qPCR profiles indicated flower-specific (DnoARF5, DnoARF6, DnoARF16, DnoARF22), which suggest an important impact regulation. Ultimately, study provides comprehensive elucidate functions species suggests new insights further exploration concerning function regulatory mechanisms during development.

Language: Английский

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An ultra-conserved ARF-DNA interface underlies auxin-triggered transcriptional response

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 2, 2024

Abstract Auxin Response Factor (ARF) plant transcription factors are the key effectors in auxin signalling. Their DNA-Binding Domain (DBD) contains a B3 domain that allows base-specific interactions with Elements (AuxREs) DNA target sites. Land plants encode three phylogenetically distinct ARF classes: closely related A- and B-classes have overlapping binding properties, contrasting different DNA-binding properties of divergent C-class ARFs. divergence likely occurred early evolution gene family, but molecular determinants underlying it remain unclear. Here, we show residues deeply conserved ARFs, variability within these is only present tracheophytes, correlating greatly expanded families. Using liverwort Marchantia polymorpha , confirm essential role DNA-contacting for function. We further B3-AuxRE interfaces not mutation-tolerant, suggesting low evolvability has led to ultra-conservation B3-DNA interface between classes. Our data support almost complete interchangeability A/B-class by performing interspecies swaps, even lineages diverged over half billion years ago. analysis suggests specificity during common streptophyte ancestor, followed strong selection as part competition-based response system Significance Statement evolved nearly ago earliest land plants. mediated family factors. It been unclear if how evolved. In this paper, authors central protein-DNA defines genes under control remained essentially unchanged throughout evolution, explaining become dominant signal controlling growth development all

Language: Английский

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Current opinions on auxin research and its application in soybean breeding

Scientia Sinica Vitae, Journal Year: 2023, Volume and Issue: unknown

Published: July 28, 2023

Language: Английский

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Genome-Wide Identification and Expression Analysis of Auxin Response Factor (ARF) Gene Family in Betula pendula

Horticulturae, Journal Year: 2023, Volume and Issue: 10(1), P. 27 - 27

Published: Dec. 28, 2023

As the key transcription factors regulating auxin responsive genes expression, response (ARFs) play critical roles in diverse aspects of plant growth and development. Betula pendula is a valuable ornamental tree, information on ARF gene family B. needed for better understanding. The publication genome sequence enable to analyze bioinformatics expression pattern BpeARF genome-wide basis. In this study, physical chemical properties, chromosome location, phylogenetic relationship, structure, conserved domain, motif composition, cis-acting element were analyzed, patterns investigated using completely random design different tissues under exogenous NAA drought treatments. A total 17 was identified from genome, which unevenly distributed 13 chromosomes encoded adequate proteins ranging 613 1135 amino acids length. Three pairs formed by segmental duplication, Ka/Ks values these less than 1. According relationship among pendula, platyphylla, Populus trichocarpa, Arabidopsis thaliana, divided into four classes, intron/exon composition showed high similarity within same class. elements promoter regions related tissue development, hormone response, stress resistance. Quantitative real-time PCR exhibited treatment stress. expressions one, ten, seven, three levels buds, young leaves, stems, roots, respectively. Under treatment, six stems roots upregulated at all timepoints. stress, BpeARF7 BpeARF15 BpeARF5 BpeARF6 downregulated roots. Our results provided classification putative functions family, may be helpful selecting candidate verifying function genetic engineering birch trees further research.

Language: Английский

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