Parameter optimisation for mitigating somatosensory confounds during transcranial ultrasonic stimulation DOI Creative Commons
Benjamin R. Kop,

Linda de Jong,

Kim Butts Pauly

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: March 19, 2025

Transcranial ultrasonic stimulation (TUS) redefines what is possible with non-invasive neuromodulation by oaering unparalleled spatial precision and flexible targeting capabilities. However, peripheral confounds pose a significant challenge to reliably implementing this technology. While auditory during TUS have been studied extensively, the somatosensory confound has overlooked thus far. It will become increasingly vital quantify manage as field shifts towards higher doses, more compact devices, frequent through temple where co-stimulation pronounced. Here, we provide systematic characterisation of TUS. We also identify conditions under which can be mitigated most eaectively mapping confound-parameter space. Specifically, investigate dose-response eaects, pulse shaping characteristics, transducer-specific parameters. demonstrate that avoiding near-field intensity peaks in scalp, spreading energy across greater area ramping envelope, delivering equivalent doses via longer, lower-intensity pulses rather than shorter, higher-intensity pulses. Additionally, repetition frequencies fundamental reduce eaects. Through our parameter space, find preliminary evidence particle displacement (strain) may primary biophysical driving force behind co-stimulation. This study provides actionable strategies minimise confounds, support thorough experimental control required unlock full potential for scientific research clinical interventions. Tactile, thermal, even painful occur TUS.Confounds & parameters.Valid replicable requires confounds.Particle confounds.

Language: Английский

Optimized ultrasound neuromodulation for non-invasive control of behavior and physiology DOI

Keith R. Murphy,

Jordan S. Farrell, Jonas Bendig

et al.

Neuron, Journal Year: 2024, Volume and Issue: 112(19), P. 3252 - 3266.e5

Published: July 29, 2024

Language: Английский

Citations

21
Exploiting the mechanical effects of ultrasound for noninvasive therapy DOI
Meaghan A. O’Reilly

Science, Journal Year: 2024, Volume and Issue: 385(6714)

Published: Sept. 12, 2024

Focused ultrasound is a platform technology capable of eliciting wide range biological responses with high spatial precision deep within the body. Although focused already in clinical use for focal thermal ablation tissue, there has been recent growth development and translation ultrasound-mediated nonthermal therapies. These approaches exploit physical forces to produce dependent on exposure conditions. This review discusses advances four application areas that have seen particular immense potential: brain drug delivery, neuromodulation, tissue destruction, endogenous immune system activation. Owing maturation transcranial technology, major target organ; however, indications outside are also discussed.

Language: Английский

Citations

19
Biophysical effects and neuromodulatory dose of transcranial ultrasonic stimulation. DOI
Tulika Nandi, Benjamin R. Kop, Kasra Naftchi-Ardebili

et al.

Brain stimulation, Journal Year: 2025, Volume and Issue: unknown

Published: March 1, 2025

Language: Английский

Citations

2
Nanobubble-actuated ultrasound neuromodulation for selectively shaping behavior in mice DOI Creative Commons
Xuandi Hou,

Jianing Jing,

Yizhou Jiang

et al.

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: March 13, 2024

Abstract Ultrasound is an acoustic wave which can noninvasively penetrate the skull to deep brain regions, enabling neuromodulation. However, conventional ultrasound’s spatial resolution diffraction-limited and low-precision. Here, we report nanobubble-mediated ultrasound stimulation capable of localizing effects only desired region in male mice. By varying delivery site nanobubbles, could activate specific regions mouse motor cortex, evoking EMG signaling limb movement, also, separately, one two nearby elicit distinct behaviors (freezing or rotation). Sonicated neurons displayed reversible, low-latency calcium responses increased c-Fos expression sub-millimeter-scale with nanobubbles present. relevant also modified depression-like behavior a model. We provide evidence role for mechanosensitive ion channels. Altogether, our treatment scheme allows spatially-targetable, repeatable temporally-precise activation circuits neuromodulation without needing genetic modification.

Language: Английский

Citations

15
Sonogenetics for Monitoring and Modulating Biomolecular Function by Ultrasound DOI Creative Commons
Johannes Hahmann, Aman Ishaqat, Twan Lammers

et al.

Angewandte Chemie International Edition, Journal Year: 2024, Volume and Issue: 63(13)

Published: Jan. 10, 2024

Abstract Ultrasound technology, synergistically harnessed with genetic engineering and chemistry concepts, has started to open the gateway remarkable realm of sonogenetics—a pioneering paradigm for remotely orchestrating cellular functions at molecular level. This fusion not only enables precisely targeted imaging therapeutic interventions, but also advances our comprehension mechanobiology unparalleled depths. Sonogenetic tools harness mechanical force within small tissue volumes while preserving integrity surrounding physiological environment, reaching depths up tens centimeters high spatiotemporal precision. These capabilities circumvent inherent physical limitations alternative in vivo control methods such as optogenetics magnetogenetics. In this review, we first discuss mechanosensitive ion channels, most commonly utilized sonogenetic mediators, both mammalian non‐mammalian systems. Subsequently, provide a comprehensive overview state‐of‐the‐art approaches that leverage thermal or features ultrasonic waves. Additionally, explore strategies centered around design mechanochemically reactive macromolecular Furthermore, delve into ultrasound biomolecular function, encompassing utilization gas vesicles acoustic reporter genes. Finally, shed light on challenges sonogenetics present perspective future promising technology.

Language: Английский

Citations

13
A systematic review of preclinical and clinical transcranial ultrasound neuromodulation and opportunities for functional connectomics DOI Creative Commons
Carly Pellow, Samuel Pichardo, G. Bruce Pike

et al.

Brain stimulation, Journal Year: 2024, Volume and Issue: 17(4), P. 734 - 751

Published: June 15, 2024

Low-intensity transcranial ultrasound has surged forward as a non-invasive and disruptive tool for neuromodulation with applications in basic neuroscience research the treatment of neurological psychiatric conditions. To provide comprehensive overview update preclinical clinical low intensity emphasize emerging role functional brain mapping to guide, better understand, predict responses. A systematic review was conducted by searching Web Science Scopus databases studies on neuromodulation, both humans animals. 187 relevant were identified reviewed, including 116 71 reports subjects belonging diverse cohorts. Milestones are described within an broader landscape. General neural readouts outcome measures discussed, potential confounds noted, use magnetic resonance imaging is highlighted. Ultrasound emerged powerful study treat range conditions its combination various significantly advanced this platform. In particular, yielded exciting inferences into advance our understanding function, neuromodulatory mechanisms, ultimately outcomes. It anticipated that these trials first many; focused ultrasound, particularly imaging, enhance spectrum

Language: Английский

Citations

10
The relationship between parameters and effects in transcranial ultrasonic stimulation. DOI Creative Commons
Tulika Nandi, Benjamin R. Kop,

Kim Butts Pauly

et al.

Brain stimulation, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 1, 2024

Language: Английский

Citations

9
Mechanosensitive Piezo1 channel in physiology and pathophysiology of the central nervous system DOI Creative Commons
Boyi Zong,

Fengzhi Yu,

Xiaoyou Zhang

et al.

Ageing Research Reviews, Journal Year: 2023, Volume and Issue: 90, P. 102026 - 102026

Published: July 31, 2023

Since the discovery of mechanosensitive Piezo1 channel in 2010, there has been a significant amount research conducted to explore its regulatory role physiology and pathology various organ systems. Recently, growing body compelling evidence emerged linking activity health disease central nervous system. However, exact mechanisms underlying these associations remain inadequately comprehended. This review systematically summarizes current on implications for system mechanobiology, retrospects results demonstrating cell types within system, including neural stem cells, neurons, oligodendrocytes, microglia, astrocytes, brain endothelial cells. Furthermore, discusses understanding involvement disorders, such as Alzheimer's disease, multiple sclerosis, glaucoma, stroke, glioma.

Language: Английский

Citations

19
Low‐Frequency Ultrasound Sensitive Piezo1 Channels Regulate Keloid‐Related Characteristics of Fibroblasts DOI Creative Commons
Zixi Jiang, Ziyan Chen, Yantao Xu

et al.

Advanced Science, Journal Year: 2024, Volume and Issue: 11(14)

Published: Feb. 4, 2024

Abstract Keloids are benign fibroproliferative tumors that severely diminish the quality of life due to discomfort, dysfunction, and disfigurement. Recently, ultrasound technology as a noninvasive adjuvant therapy is developed optimize treatment protocols. However, biophysical mechanisms have not yet been fully elucidated. Here, it proposed piezo‐type mechanosensitive ion channel component 1 (Piezo1) plays an important role in low‐frequency sonophoresis (LFS) induced mechanical transduction pathways trigger downstream cellular signaling processes. It demonstrated patient‐derived primary keloid fibroblasts (PKF), NIH 3T3, HFF‐1 cell migration inhibited, PKF apoptosis significantly increased by LFS stimulation. And effects diminished application GsMTx‐4, selective inhibitor Piezo1, knockdown Piezo1. More importantly, can be imitated Yoda1, agonist Piezo1 channels. Establishing xenograft implantation mouse model further verified these results, decreased volume weight keloids. Moreover, blocking impaired effectiveness treatment. These results suggest inhibits malignant characteristics keloids activating channel, thus providing theoretical basis for improving clinical

Language: Английский

Citations

8
Progress in Noninvasive Low-Intensity Focused Ultrasound Neuromodulation DOI
Jinru Feng, Zixiao Li

Stroke, Journal Year: 2024, Volume and Issue: 55(10), P. 2547 - 2557

Published: Aug. 15, 2024

Low-intensity focused ultrasound represents groundbreaking medical advancements, characterized by its noninvasive feature, safety, precision, and broad neuromodulatory capabilities. This technology operates through mechanisms, for example, acoustic radiation force, cavitation, thermal effects. Notably, with the evolution of technology, neuromodulation has been gradually applied in treating central nervous system diseases, especially stroke. Furthermore, burgeoning research areas such as sonogenetics nanotechnology show promising potential. Despite benefit low-intensity precise biophysical mechanism still need further exploration. review discusses recent ongoing developments neurological regulation, covering underlying rationale to current utility challenges that impede development broader adoption this alternative therapy.

Language: Английский

Citations

7