RAC1 serves as a prognostic factor and correlated with immune infiltration in liver hepatocellular carcinoma DOI Creative Commons
Yuan Li, Aidong Gu, Lili Yang

et al.

Journal of Cancer Research and Clinical Oncology, Journal Year: 2024, Volume and Issue: 150(9)

Published: Sept. 12, 2024

Language: Английский

Human CSPG4-targeting CAR-macrophages inhibit melanoma growth DOI Creative Commons
Daniel Greiner, Qian Xue, Trinity Q Alaka’i Waddell

et al.

Oncogene, Journal Year: 2025, Volume and Issue: unknown

Published: March 13, 2025

Abstract Approximately half of melanoma patients relapse or fail to respond current standards care, highlighting the need for new treatment options. Engineering T-cells with chimeric antigen receptors (CARs) has revolutionized hematological malignancies but been clinically less effective in solid tumors. We therefore sought engineer alternative immune cell types inhibit progression. macrophages CARs emerged as a promising approach overcome some challenges faced by CAR-T cells; however, whether these engineered can effectively growth is unknown. To determine CAR-macrophages (CAR-Ms) specifically target and kill cells, we CAR-Ms targeting chondroitin sulfate proteoglycan 4 (CSPG4), an expressed melanoma. CSPG4-targeting exhibited specific phagocytosis CSPG4-expressing cells. developed 3D approaches show that efficiently infiltrated spheroids. Furthermore, combining strategies inhibiting CD47/SIRPα “don’t eat me” signaling synergistically enhanced CAR-M-mediated robustly inhibited spheroid 3D. Importantly, tumor mouse models. These results suggest engineering against antigens immunotherapy treating

Language: Английский

Citations

2
Clinical and functional spectrum of RAC2-related immunodeficiency DOI

Ágnes Donkó,

Svetlana O. Sharapova, Juraj Kabát

et al.

Blood, Journal Year: 2024, Volume and Issue: 143(15), P. 1476 - 1487

Published: Jan. 9, 2024

Language: Английский

Citations

11
Plasma membrane abundance dictates phagocytic capacity and functional cross-talk in myeloid cells DOI
Benjamin Y. Winer, Alexander Settle,

Alexandrina M. Yakimov

et al.

Science Immunology, Journal Year: 2024, Volume and Issue: 9(96)

Published: June 7, 2024

Professional phagocytes like neutrophils and macrophages tightly control what they consume, how much when move after cargo uptake. We show that plasma membrane abundance is a key arbiter of these cellular behaviors. Neutrophils lacking the G protein subunit Gβ

Language: Английский

Citations

8
Hyperactive Rac stimulates cannibalism of living target cells and enhances CAR-M-mediated cancer cell killing DOI Creative Commons
Abhinava K. Mishra, Melanie M. Domenech Rodríguez, Alba Yurani Torres

et al.

Proceedings of the National Academy of Sciences, Journal Year: 2023, Volume and Issue: 120(52)

Published: Dec. 18, 2023

The 21kD GTPase Rac is an evolutionarily ancient regulator of cell shape and behavior. Rac2 predominantly expressed in hematopoietic cells where it essential for survival motility. hyperactivating mutation E62K also causes human immunodeficiency, although the mechanism remains unexplained. Here, we report that Drosophila, stimulates ovarian to cannibalize neighboring cells, destroying tissue. We then show hyperactive HL60-derived macrophage-like engulf kill living T leukemia cells. Primary mouse +/E62K bone-marrow-derived macrophages primary due a combination macrophage hyperactivity hypersensitivity engulfment. Additionally, non-autonomously stimulate wild-type enhances engulfment target cancer by chimeric antigen receptor-expressing (CAR-M) CAR-dependent manner. propose Rac-mediated cannibalism may contribute immunodeficiency enhance CAR-M immunotherapy.

Language: Английский

Citations

15
Ras suppression potentiates rear actomyosin contractility-driven cell polarization and migration DOI
Yiyan Lin, Dhiman Sankar Pal,

Parijat Banerjee

et al.

Nature Cell Biology, Journal Year: 2024, Volume and Issue: 26(7), P. 1062 - 1076

Published: July 1, 2024

Language: Английский

Citations

5
Target cell adhesion limits macrophage phagocytosis and promotes trogocytosis DOI Creative Commons
Kirstin Rollins, Sareen Fiaz, Meghan A. Morrissey

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 8, 2025

Abstract Macrophage phagocytosis is an essential immune response that eliminates pathogens, antibody-opsonized cancer cells and debris. Macrophages can also trogocytose, or nibble, targets. Trogocytosis are often activated by the same signal, including IgG antibodies. What makes a macrophage trogocytose instead of phagocytose not clear. Using both CD47 antibodies Her2 Chimeric Antigen Receptor (CAR) to induce phagocytosis, we found macrophages preferentially adherent target in 2D cell monolayers 3D spheroid models. Disrupting integrin using RGD peptide through CRISPR-Cas9 knockout αV subunit increased phagocytosis. Conversely, increasing adhesion ectopically expressing E-Cadherin Raji B targets reduced Finally, examined mitotic cells, naturally occurring example with adhesion. Arresting mitosis significantly Together, our data show limits promotes trogocytosis.

Language: Английский

Citations

0
Taraxacum mongolicum Hand.-Mazz extract disrupts the interaction between triple-negative breast cancer cells and tumor-associated macrophages by inhibiting RAC2/NF-κB p65/p38 MAPK pathway DOI
Xinrui Li,

Yang Guo,

Xinxin Deng

et al.

Journal of Ethnopharmacology, Journal Year: 2025, Volume and Issue: unknown, P. 119757 - 119757

Published: April 1, 2025

Language: Английский

Citations

0
Research progress of targeted BCMA CAR-T therapy for relapsed/refractory multiple myeloma antigen-negative relapse DOI
Lulu Kong, Kailin Xu, Wei Chen

et al.

Best Practice & Research Clinical Haematology, Journal Year: 2025, Volume and Issue: unknown, P. 101632 - 101632

Published: May 1, 2025

Language: Английский

Citations

0
Signaling of Phagocytosis DOI

Abdolrasol K Panah,

Valentin Jaumouillé

Elsevier eBooks, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Citations

0
Probiotics alleviate paraneoplastic thrombocythemia of ovarian cancer: A randomized placebo-controlled trial DOI Creative Commons

Xiaoni Zhou,

Gang Hu,

Zhaowei Luo

et al.

Journal of Functional Foods, Journal Year: 2024, Volume and Issue: 119, P. 106316 - 106316

Published: June 25, 2024

Paraneoplastic thrombocytosis is common in ovarian cancer and often correlates with disease recurrence. This study aimed to assess the impact of probiotics on platelet counts patients. Enrolled patients paraneoplastic received oral Bifidobacterium longum JBLC-141 for level modulation. Over a 12-month period, were closely monitored recurrence rates. The revealed significant differences between probiotic placebo groups various parameters, including counts, coagulation functions, inflammatory factors, tumor markers, gut microbiota composition. However, no distinctions observed during postoperative stabilization. Oral effectively mitigated count surges improved functions thrombocytosis. Positive effects inflammation, marker levels, composition also noted. Further clinical validation needed confirm probiotics' potential extending stabilization period.

Language: Английский

Citations

2