Toward telomere-to-telomere cat genomes for precision medicine and conservation biology DOI
William J. Murphy, Andrew J. Harris

Genome Research, Journal Year: 2024, Volume and Issue: 34(5), P. 655 - 664

Published: May 1, 2024

Genomic data from species of the cat family Felidae promise to stimulate veterinary and human medical advances, clarify coherence genome organization. We describe how interspecies hybrids have been instrumental in genetic analysis cats, first maps propelling genomes toward T2T standard set by project. Genotype-to-phenotype mapping models has revealed dozens health-related variants, molecular basis for mammalian pigmentation patterning, species-specific adaptations. Improved genomic surveillance natural captive populations across tree will increase our understanding architecture traits, population dynamics, guide a future genome-enabled biodiversity conservation.

Language: Английский

Application of Next-Generation Sequencing (NGS) Techniques for Selected Companion Animals DOI Creative Commons
Kinga Domrazek, P. Jurka

Animals, Journal Year: 2024, Volume and Issue: 14(11), P. 1578 - 1578

Published: May 27, 2024

Next-Generation Sequencing (NGS) techniques have revolutionized veterinary medicine for cats and dogs, offering insights across various domains. In parasitology, NGS enables comprehensive profiling of parasite populations, aiding in understanding transmission dynamics drug resistance mechanisms. infectious diseases, facilitates rapid pathogen identification, characterization virulence factors, tracking outbreaks. Moreover, sheds light on metabolic processes by elucidating gene expression patterns pathways, essential diagnosing disorders designing tailored treatments. autoimmune helps identify genetic predispositions molecular mechanisms underlying immune dysregulation. Veterinary oncology benefits from through personalized tumor profiling, mutation analysis, identification therapeutic targets, fostering precision approaches. Additionally, plays a pivotal role genetics, unraveling the basis inherited diseases facilitating breeding programs healthier animals. Physiological investigations leverage to explore complex biological systems, gene–environment interactions pathways governing health disease. Application treatment planning enhances efficacy enabling strategies individual animals their ultimately advancing care companion

Language: Английский

Citations

7
The genome of the black-footed cat: Revealing a rich natural history and urgent conservation priorities for small felids DOI

Jiaqing Yuan,

Andrew C. Kitchener, Laurie Bingaman Lackey

et al.

Proceedings of the National Academy of Sciences, Journal Year: 2024, Volume and Issue: 121(2)

Published: Jan. 2, 2024

Habitat degradation and loss of genetic diversity are common threats faced by almost all today’s wild cats. Big cats, such as tigers lions, great concern have received considerable conservation attention through policies international actions. However, knowledge actions for small cats lagging considerably behind. The black-footed cat, Felis nigripes , one the smallest felid species, is experiencing increasing with a rapid reduction in population size. there lack information to assist developing effective A de novo assembly high-quality chromosome-level reference genome cat was made, comparative genomics analyses were carried out. These revealed that most significant changes evolution sensory metabolic-related genes, reflecting adaptations its characteristic nocturnal hunting high metabolic rate. Genomes exhibit level inbreeding, especially signals recent inbreeding events, which suggest they may experienced severe isolation caused habitat fragmentation. More importantly, associated two deleterious mutated genes exacerbate risk amyloidosis, dominant disease causes mortality about 70% captive individuals. Our research provides comprehensive documentation evolutionary history suggests an urgent need investigate genomic variations felids worldwide support

Language: Английский

Citations

6
Gene pseudogenization in fertility-associated genes in cheetah (Acinonyx jubatus), a species with long-term low effective population size DOI Creative Commons
Jessica A. Peers, Will Nash, Wilfried Haerty

et al.

Evolution, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 17, 2025

We are witnessing an ongoing global biodiversity crisis, and increasing number of mammalian populations at risk decline. Species that have survived severe historic bottlenecks, such as the cheetah (Acinonyx jubatus) exhibit symptoms inbreeding depression including reproductive developmental defects. Although it has long been suggested defects stem from accumulation weakly deleterious mutations, implications mutations leading to pseudogenization not assessed. Here, we use comparative analysis eight felid genomes better understand impacts in cheetah. find novel events specific Through careful curation, identify 65 genes with previously unreported premature termination codons likely affect gene function. With addition population data (n=6) 22 these least one resequenced individual, four which (DEFB116, ARL13A, CFAP119 NT5DC4) also found a more recent reference genome. Mutations within three linked sterility, azoospermia, is common cheetahs. Our results highlight power genomic approaches for discovery causative variants declining species.

Language: Английский

Citations

0
Genome-Wide Association Study Reveals Genetic Mechanisms Underlying Intersex and Aproctia in Large White Pigs DOI Creative Commons
Yajun Li, Jiaxin Shi,

Yingshan Yang

et al.

Animals, Journal Year: 2025, Volume and Issue: 15(8), P. 1094 - 1094

Published: April 10, 2025

Congenital developmental abnormalities in piglets, such as intersex and aproctia, adversely affect survival rates, growth performance, genetic breeding efficiency pig populations. To elucidate their basis, we performed a genome-wide association study (GWAS) on 1030 Large White pigs. We combined 50 K SNP chip data with SWIM-based genotype imputation to enhance the resolution of variation detection, followed by MLM analysis. Our results identified 53 significant SNPs, 52 associated 1 aproctia. Key candidate genes included MAD1L1, ID4, EFNA5, PPP1R16B for ARNT2 Functional enrichment analysis highlighted pathways related gonadal development (e.g., progesterone-mediated oocyte maturation) embryonic morphogenesis. Collectively, identification these SNPs advances our understanding architecture aproctia piglets. These findings provide actionable insights optimizing strategies improving health management production, potential implications reducing economic losses caused congenital disorders.

Language: Английский

Citations

0
The effect of the demographic history on the evolution of senescence: A potential new test of the mutation accumulation theory DOI
Guillaume Péron

Mechanisms of Ageing and Development, Journal Year: 2024, Volume and Issue: 219, P. 111927 - 111927

Published: March 16, 2024

Language: Английский

Citations

0
Toward telomere-to-telomere cat genomes for precision medicine and conservation biology DOI
William J. Murphy, Andrew J. Harris

Genome Research, Journal Year: 2024, Volume and Issue: 34(5), P. 655 - 664

Published: May 1, 2024

Genomic data from species of the cat family Felidae promise to stimulate veterinary and human medical advances, clarify coherence genome organization. We describe how interspecies hybrids have been instrumental in genetic analysis cats, first maps propelling genomes toward T2T standard set by project. Genotype-to-phenotype mapping models has revealed dozens health-related variants, molecular basis for mammalian pigmentation patterning, species-specific adaptations. Improved genomic surveillance natural captive populations across tree will increase our understanding architecture traits, population dynamics, guide a future genome-enabled biodiversity conservation.

Language: Английский

Citations

0