Multiscale Mass Transport Across Membranes: From Molecular Scale to Nanoscale to Micron Scale DOI

Guanhua Xu,

Ao Chen, Feng Feng

et al.

ACS Nano, Journal Year: 2024, Volume and Issue: 18(52), P. 35347 - 35355

Published: Dec. 19, 2024

Multiscale mass transport across membranes occurs ubiquitously in biological systems but is difficult to achieve and long-sought-after abiotic systems. The multiscale transmembrane requires the integration of channels energy ergodicity, making a significant challenge. Herein, emulsion droplets with cell-like confinement are used as experimental model, achieved from molecular scale nanoscale micron scale, reproducing rudimentary forms behaviors. By adjustment magnetic dipole interactions between adjacent superparamagnetic nanoparticles (MNPs), assembled structure at interface successfully modified, which constructs various scales interface. Simultaneously, assembly process MNPs induces self-emulsification, increases entropy further reduces Gibbs free energy, ultimately realizing that evolves time visiting all possible microscopic states (energy ergodicity). This work represents comprehensive identification realization droplet systems, offers opportunities for development high-order characteristics droplet-based communities, synthetic cells, microrobots, drug carriers.

Language: Английский

Remodeling of Biomembranes and Vesicles by Adhesion of Condensate Droplets DOI Creative Commons
Reinhard Lipowsky

Membranes, Journal Year: 2023, Volume and Issue: 13(2), P. 223 - 223

Published: Feb. 10, 2023

Condensate droplets are formed in aqueous solutions of macromolecules that undergo phase separation into two liquid phases. A well-studied example the polymers PEG and dextran which have been used for a long time biochemical analysis biotechnology. More recently, has also observed living cells where it leads to membrane-less or droplet-like organelles. In latter case, condensate enriched certain types proteins. Generic features can be studied simple binary mixtures, using molecular dynamics simulations. this review, I address interactions with biomimetic biological membranes. When droplet adheres such membrane, membrane forms contact line acquires very high curvature close line. The angles along via light microscopy, lead classification possible adhesion morphologies, determine affinity contrast between coexisting phases membrane. remodeling processes generated by include wetting transitions, formation nanotubes as well complete engulfment endocytosis

Language: Английский

Citations

14
Lipid packing and cholesterol content regulate membrane wetting and remodeling by biomolecular condensates DOI Creative Commons
Agustín Mangiarotti, Elias Sabri,

Kita Valerie Schmidt

et al.

Nature Communications, Journal Year: 2025, Volume and Issue: 16(1)

Published: March 20, 2025

Abstract Biomolecular condensates play a central role in cellular processes by interacting with membranes driving wetting transitions and inducing mutual remodeling. While are known to locally alter membrane properties such as lipid packing hydration, it remains unclear how composition phase state turn affect condensate affinity. Here, we show that is not only the itself, but rather degree of determines affinity for membranes. Increasing chain length, saturation, or cholesterol content, enhances packing, thereby decreasing interaction. This regulatory mechanism consistent across various condensate-membrane systems, highlighting critical interface. In addition, protein adsorption promotes extensive remodeling, including formation tubes double-membrane sheets. Our findings reveal which fine-tunes wetting, its potential impact on functions organelle interactions.

Language: Английский

Citations

0
Lipid packing and cholesterol content regulate membrane wetting by biomolecular condensates DOI Creative Commons
Agustín Mangiarotti, Elias Sabri,

Kita Valerie Schmidt

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: July 18, 2024

Abstract Biomolecular condensates play a pivotal role in cellular processes by interacting with membranes and leading to wetting transitions mutual remodeling. Using combination of hyperspectral imaging, phasor analysis, fluid-elastic parameter measurements, we investigated how membrane lipid packing affects condensate wetting. Our results show that it is not only the phase state, but rather degree determines affinity for membranes. Increasing chain length or cholesterol content enhances packing, thereby decreasing affinity. This regulatory mechanism consistent across various condensate-membrane systems, underscoring critical interface. Additionally, protein adsorption promotes extensive remodeling, including tube double-membrane sheet formation. work provides novel which composition fine-tunes wetting, highlighting its potential impact on functions organelle interactions.

Language: Английский

Citations

3
Multiscale Mass Transport Across Membranes: From Molecular Scale to Nanoscale to Micron Scale DOI

Guanhua Xu,

Ao Chen, Feng Feng

et al.

ACS Nano, Journal Year: 2024, Volume and Issue: 18(52), P. 35347 - 35355

Published: Dec. 19, 2024

Multiscale mass transport across membranes occurs ubiquitously in biological systems but is difficult to achieve and long-sought-after abiotic systems. The multiscale transmembrane requires the integration of channels energy ergodicity, making a significant challenge. Herein, emulsion droplets with cell-like confinement are used as experimental model, achieved from molecular scale nanoscale micron scale, reproducing rudimentary forms behaviors. By adjustment magnetic dipole interactions between adjacent superparamagnetic nanoparticles (MNPs), assembled structure at interface successfully modified, which constructs various scales interface. Simultaneously, assembly process MNPs induces self-emulsification, increases entropy further reduces Gibbs free energy, ultimately realizing that evolves time visiting all possible microscopic states (energy ergodicity). This work represents comprehensive identification realization droplet systems, offers opportunities for development high-order characteristics droplet-based communities, synthetic cells, microrobots, drug carriers.

Language: Английский

Citations

0