Biomolecular Condensates can Induce Local Membrane Potentials DOI Creative Commons
Anthony Gurunian, Keren Lasker, Ashok A. Deniz

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 28, 2024

Abstract Biomolecular condensates are a ubiquitous component of cells, known for their ability to selectively partition and compartmentalize biomolecules without the need lipid membrane. Nevertheless, have been shown interact with membranes in diverse biological processes, such as autophagy T-cell activation. Since many net surface charge density associated electric potential(s), we hypothesized that they can induce local membrane potential. Using an electrochromic dye, demonstrate poly-lysine/ATP localized potential Giant Unilamellar Vesicles. This effect diminishes increasing salt concentration higher ATP-to-poly-lysine ratios, underscoring key role condensate charge. Numerical modeling condensate-membrane interface using electro-thermodynamic framework supports our experimental findings highlights parameters expected play effect. These results broad implications processes regulated by potential, particularly contexts neuronal signaling, where interactions may previously unrecognized regulatory role.

Language: Английский

Phosphorylation Toggles the SARS-CoV-2 Nucleocapsid Protein Between Two Membrane-Associated Condensate States DOI Creative Commons
Bruna Favetta, Huan Wang, Jasmine Cubuk

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 18, 2024

The SARS-CoV-2 Nucleocapsid protein (N) performs several functions during the viral lifecycle, including transcription regulation and genome encapsulation. We hypothesized that N toggles between these via phosphorylation-induced conformational change, thereby altering interactions with membranes RNA. found phosphorylation changes how biomolecular condensates composed of RNA interact membranes: phosphorylated (pN) form thin films, while unmodified are engulfed. This partly results from in material properties, pN forming less viscous elastic condensates. weakening protein-RNA interaction upon is driven by a decrease binding unstructured show induces change serine/arginine-rich region increases monomers decreases nonspecific These findings connect conformation, membrane-associated states N, potential implications for COVID-19 treatment.

Language: Английский

Citations

2
Two-dimensional condensates of HRS drive the assembly of flat clathrin lattices on endosomes DOI Creative Commons
Markku Hakala, Satish Babu Moparthi, Iva Ganeva

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 1, 2024

Abstract Amongst the different clathrin structures in mammalian cells, bi-layered coat colocalizing with endosomal sorting complex required for transport (ESCRT)-0 remains one of most ambiguous. Despite being observed first time twenty years ago, their structure and how they are assembled unknown. Here, we reconstituted vitro ESCRT-0 assembly onto various types membranes. The protein HRS, a known adaptor on endosomes, was found to form condensates. These condensates spread into thin layer PI(3)P-rich Platinum replica electron microscopy revealed that, surprisingly, depending HRS phase. Protein droplets recruited as dense, curved lattice, many cage-like structures. On two-dimensional condensates, dense flat assembly. Two-dimensional HRS-clathrin promoted clustering cholesterol underlying membrane, while enhanced PI(3)P- dependent recruitment membrane. free-standing membranes, membrane flattening. Overall, these results show that two- dimensional condensate creates unique cargo molecules, defining new mechanism trafficking processes.

Language: Английский

Citations

0
Biomolecular Condensates can Induce Local Membrane Potentials DOI Creative Commons
Anthony Gurunian, Keren Lasker, Ashok A. Deniz

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 28, 2024

Abstract Biomolecular condensates are a ubiquitous component of cells, known for their ability to selectively partition and compartmentalize biomolecules without the need lipid membrane. Nevertheless, have been shown interact with membranes in diverse biological processes, such as autophagy T-cell activation. Since many net surface charge density associated electric potential(s), we hypothesized that they can induce local membrane potential. Using an electrochromic dye, demonstrate poly-lysine/ATP localized potential Giant Unilamellar Vesicles. This effect diminishes increasing salt concentration higher ATP-to-poly-lysine ratios, underscoring key role condensate charge. Numerical modeling condensate-membrane interface using electro-thermodynamic framework supports our experimental findings highlights parameters expected play effect. These results broad implications processes regulated by potential, particularly contexts neuronal signaling, where interactions may previously unrecognized regulatory role.

Language: Английский

Citations

0