Assessment of multiple hemorheological properties in microfluidic environment DOI
Yang Jun Kang

Physics of Fluids, Journal Year: 2024, Volume and Issue: 36(12)

Published: Dec. 1, 2024

Measuring and monitoring hemorheological properties provide valuable insights into diseases. To effectively detect impaired blood, it is necessary to quantify the multiple properties. However, most of previous methods only single blood property. They require bulky expensive syringe pumps for precise on–off control. In this study, resolve several issues, a novel method measuring (fluidic resistance, viscosity, time constant, compliance coefficient, red cell [RBC] aggregation index, RBC sedimentation index) proposed by analyzing images in microfluidic channels, where transient flow induced portable air-compression pump. A device consists an inlet, test chamber joined main channel, reservoir. The outlet connected air damper, which contributes stopping promptly. fluid circuit model channels constructed estimating rate pressure channel. First, used obtain rheological glycerin solution (30%). normalized difference between reference value less than 4%. Subsequently, adopted differences medium (1× phosphate-buffered saline, dextran solution: 20 mg/ml) hematocrit (30%–60%). All exhibit substantial with respect medium. yields comparable results when compared methods. conclusion, can measure channels.

Language: Английский

Diagnostic potential of blood plasma longitudinal viscosity measured using Brillouin light scattering DOI Creative Commons
Jennifer Illibauer,

Tamara Clodi-Seitz,

Alexander Zoufaly

et al.

Proceedings of the National Academy of Sciences, Journal Year: 2024, Volume and Issue: 121(33)

Published: Aug. 1, 2024

Blood plasma viscosity (PV) is an established biomarker for numerous diseases. Measurement of the shear PV using conventional rheological techniques is, however, time consuming and requires significant volumes. Here, we show that Brillouin light scattering (BLS) angle-resolved spectroscopy measurements longitudinal from microliter-sized volumes can serve as a proxy measured viscometers. This not trivial given distinct frequency regime probed viscosity, combination bulk representing unique material property on account latter. We demonstrate this healthy persons patients suffering different severities COVID-19 (CoV), which has been associated with increased PV. further additional information contained in BLS-measured effective its temperature scaling provide insight into chemical constituents physical properties be diagnostic value. In particular, find changes are consistent suspension concentration CoV patient samples at elevated temperatures correlated disease severity progression. supported by results rapid BLS spatial-mapping, measurements, elastic scattering, anomalies viscosity. Finally, introduce compact probe to rapidly perform plastic transport tubes. Our open broad avenue diagnostics based high-frequency means implementation.

Language: Английский

Citations

5
Microfluidic Chip for Quantitatively Assessing Hemorheological Parameters DOI Creative Commons
Yang Jun Kang

Micromachines, Journal Year: 2025, Volume and Issue: 16(5), P. 567 - 567

Published: May 8, 2025

The biomechanical properties of blood are regarded as promising biomarkers for monitoring early-stage abnormalities and disease progression. To detect any changes in blood, it is necessary to measure many rheological possible. Herein, a novel method proposed measuring multiple using microfluidic chip. syringe pump turns off 5 min induce RBC (red cell) sedimentation the driving syringe. aggregation determined by analyzing time-lapse image intensity at stasis: I(t) = I1 exp (−k1t) + I2 (−k2t). RBC-rich RBC-depleted sequentially infused into Based on pressure estimated with velocity, viscosity acquired Hagen–Poiseuille law. quantified distance (Xesr) erythrocyte rate (ESR). provides consistent compared previous methods. Two four variables (I1, I2) exhibited strong correlation conventional index (AI). indices Xesr ESR showed trends respect medium hematocrit. In conclusion, then effective properties.

Language: Английский

Citations

0
Glycocalyx Disintegration Is Associated with Mortality in Chronic Heart Failure DOI Open Access
Patricia P. Wadowski, Martin Hülsmann, Iréne Lang

et al.

Journal of Clinical Medicine, Journal Year: 2025, Volume and Issue: 14(10), P. 3571 - 3571

Published: May 20, 2025

Background: Glycocalyx disintegration is associated with adverse outcomes in patients trauma or sepsis. As microvascular dysfunction has an impact on disease progression chronic heart failure (CHF) patients, we hypothesized that changes microcirculation might be mortality. Methods: Fifty ischemic and non-ischemic cardiomyopathy conservative treatment baseline measurements of the sublingual (via Sidestream Darkfield videomicroscopy) were followed up for two years. thickness was assessed indirectly by calculation perfused boundary region (PBR). Results: Loss glycocalyx pronounced non-survivors after one, n = 10, years, 16; PBR: 2.05 μm (1.88–2.15 μm) vs. 1.87 (1.66–2.03 2.04 (1.93–2.11) 1.84 (1.62–1.97); p 0.042 0.003, respectively. Area under ROC curve analysis predictive value PBR two-year mortality 0.77 (p 0.003; SE: 0.07, CI (95%): 0.63–0.91). determined a 1.9 as best predictor (sensitivity: 0.81; specificity: 0.59). Moreover, multivariate regression revealed functional capillary density significant predictors mortality, 0.036 0.048, Conclusions: related to poor overall survival CHF patients.

Language: Английский

Citations

0
Optical characterization of molecular interaction strength in protein condensates DOI
Timon Beck, Lize-Mari van der Linden, Wade M. Borcherds

et al.

Molecular Biology of the Cell, Journal Year: 2024, Volume and Issue: 35(12)

Published: Nov. 13, 2024

Biomolecular condensates have been identified as a ubiquitous means of intracellular organization, exhibiting very diverse material properties. However, techniques to characterize these properties and their underlying molecular interactions are scarce. Here, we introduce two optical - Brillouin microscopy quantitative phase imaging (QPI) address this scarcity. We establish shift linewidth measures for average interaction dissipation strength, respectively, used QPI obtain the protein concentration within condensates. monitored response formed by FUS low-complexity domain hnRNPA1 (A1-LCD) altering temperature ion concentration. Conditions favoring separation increased shift, linewidth, In comparison solidification chemical crosslinking, ion-dependent aging had small effect on strength inside. Finally, investigated how sequence variations A1-LCD, that change driving force separation, alter physical respective Our results provide new experimental perspective Robust approaches such presented ones will be crucial understanding biological determine function dysfunction.

Language: Английский

Citations

2
Non-Stoichiometric Effects on Viscoelasticity in DGEBA-EDA Systems: Insights from Brillouin Light Scattering DOI Creative Commons
Mikołaj Pochylski

The Journal of Physical Chemistry B, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 25, 2024

The initial and final stages of the isothermal curing reaction between diglycidyl ether bisphenol A ethylenediamine were investigated by using Brillouin light scattering spectroscopy. High-frequency acoustic parameters measured for these as a function diamine molar fraction, ranging from pure prepolymer to cross-linker. Significant differences in concentration dependencies mechanical are interpreted relation changes system's viscoelastic properties. At stage reaction, exhibit notable at two characteristic nonstoichiometric compositions─one corresponding amine excess other epoxy excess. While concentrations align well with Flory's critical gel points, experimental evidence suggests they should instead be vitrification points.

Language: Английский

Citations

0
Assessment of multiple hemorheological properties in microfluidic environment DOI
Yang Jun Kang

Physics of Fluids, Journal Year: 2024, Volume and Issue: 36(12)

Published: Dec. 1, 2024

Measuring and monitoring hemorheological properties provide valuable insights into diseases. To effectively detect impaired blood, it is necessary to quantify the multiple properties. However, most of previous methods only single blood property. They require bulky expensive syringe pumps for precise on–off control. In this study, resolve several issues, a novel method measuring (fluidic resistance, viscosity, time constant, compliance coefficient, red cell [RBC] aggregation index, RBC sedimentation index) proposed by analyzing images in microfluidic channels, where transient flow induced portable air-compression pump. A device consists an inlet, test chamber joined main channel, reservoir. The outlet connected air damper, which contributes stopping promptly. fluid circuit model channels constructed estimating rate pressure channel. First, used obtain rheological glycerin solution (30%). normalized difference between reference value less than 4%. Subsequently, adopted differences medium (1× phosphate-buffered saline, dextran solution: 20 mg/ml) hematocrit (30%–60%). All exhibit substantial with respect medium. yields comparable results when compared methods. conclusion, can measure channels.

Language: Английский

Citations

0