Rationally Designed Novel Antimicrobial Peptides Targeting Chitin Synthase for Combating Soybean Phytophthora Blight DOI Open Access

Yue Ran,

Kiran Shehzadi,

Jianhua Liang

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(6), P. 3512 - 3512

Published: March 20, 2024

Soybean phytophthora blight is a severe menace to global agriculture, causing annual losses surpassing USD 1 billion. Present crop loss mitigation strategies primarily rely on chemical pesticides and disease-resistant breeding, frequently surpassed by the pathogens' quick adaptive evolution. In this urgent scenario, our research delves into innovative antimicrobial peptides characterized low drug resistance environmental friendliness. Inhibiting chitin synthase gene activity in Phytophthora sojae impairs vital functions such as growth sporulation, presenting an effective method reduce its pathogenic impact. study, we screened 16 previously tested evaluate their effects against using structure-guided design, which involves molecular docking, saturation mutagenesis, dynamics, toxicity prediction. The silico analysis identified AMP_04 with potential inhibitory sojae's synthase. Through three rounds of pin-pointed most triple mutant, TP (D10K, G11I, S14L). Molecular dynamic simulations revealed TP's stability synthase-TP complex transmembrane mechanism, employing all-atom force field. Our findings demonstrate efficacy occupying substrate-binding pocket translocation catalytic channel. Effective inhibition enzyme can be achieved. Specifically, mutant demonstrates enhanced potency decreased relative wild-type AMP_04, utilizing mechanism akin barrel-stave model during membrane translocation. Collectively, study provides new strategy that could used potent agent combatting soybean blight, contributing sustainable agricultural practices.

Language: Английский

CRISPR/Cas Technology: A Climate Saviour or a Genetic Pandora’s Box? DOI
Aditi Agarwal,

Sonika Saxena,

Sudipti Arora

et al.

Published: Jan. 1, 2024

The potential of the CRISPR/Cas technology to revolutionize genetic engineering through precise gene editing offers significant possibilities. Consequently, it has been proposed as a promising solution for addressing challenges posed by climate change. Utilizing could potentially result in development climate-resilient crops, reduction carbon emissions from livestock farming, and carbon-sequestering microbes. Nevertheless, concerns have raised regarding risks associated with this technology, such creation invasive species unintended spread modified genes into natural populations. Moreover, ethical considerations arise when contemplating manipulation world engineering. This chapter examines both advantages employing battle against change, while suggesting approaches strike balance between benefits drawbacks, ensuring sustainable application technology.

Language: Английский

Citations

2

Advancements in genetic studies of mushrooms: a comprehensive review DOI

Entaj Tarafder,

Mir Muhammad Nizamani, Samantha C. Karunarathna

et al.

World Journal of Microbiology and Biotechnology, Journal Year: 2024, Volume and Issue: 40(9)

Published: July 22, 2024

Language: Английский

Citations

2

Evaluating the intention to purchase genetically modified food by consumers in Panama DOI Creative Commons

Agzel Marín-Díaz,

Aldo Álvarez-Risco, Shyla Del-Aguila-Arcentales

et al.

Journal of Agriculture and Food Research, Journal Year: 2024, Volume and Issue: 16, P. 101194 - 101194

Published: May 9, 2024

Genetically modified (GM) foods are important because of their ability to increase crop yields, resist disease and adverse weather conditions, improve nutritional quality. However, use generates controversy concerns about food safety, benefits risks due consumption. It is therefore necessary evaluate the importance these variables. The current study evaluated variables that explain consumers' intention purchase GM in Panama. was carried out by prospective, quantitative, non-experimental, correlational research 385 consumers. A multivariate technique model proposed study. Attitude toward safety showed a negative effect on perceived from positive food. Also, Perceived presented significant Attitudes towards Finally, positively affected intention. Firms can outcomes prepare new strategies for communication products with components. governments information create education programs population foods, risks. Future studies other regions needed.

Language: Английский

Citations

2

Exploring the future of GM technology in sustainable local food systems in Colombia DOI Creative Commons

Néstor Julián Cárdenas Pardo,

Dolly Esperanza Rodríguez Robayo,

John Cristhian Fernández Lizarazo

et al.

Frontiers in Genome Editing, Journal Year: 2023, Volume and Issue: 5

Published: June 30, 2023

The security of Earth's food systems is challenged by shifting regional climates. While agricultural processes are disrupted climate change, they also play a large role in contributing to destabilizing greenhouse gases. Finding new strategies increase yields while decreasing environmental impacts essential. Tropical agriculture particularly susceptible change: local, smallholder farming, which provides majority the supply, high risk and has limited adaptation capacity. Rapid, inexpensive, intuitive solutions needed, like implementation genetically modified (GM) crops. In Latin American tropics, awareness acceptance GM technologies, opportunities test crops as part local educations, their known economic benefits, support use. However, this not all that needed for future technologies these areas: must consider social sustainability, can be unique locality. Primarily from perspective its educators, potential rural Colombian university driving explored, including type producing engineers who innovate with meet regionally-dependent cultural needs could sustainability.

Language: Английский

Citations

4

The recent genetic modification techniques for improve soil conservation, nutrient uptake and utilization DOI Creative Commons
Gideon Sadikiel Mmbando, Kelvin Ngongolo

GM crops & food, Journal Year: 2024, Volume and Issue: 15(1), P. 233 - 247

Published: July 15, 2024

Advances in genetic modification (GM) techniques have generated huge interest improving nutrient utilization, maximizing uptake, and conserving soil the pursuit of sustainable agriculture. Unfortunately, little is still known about recent advancements application GM tactics to enhance each these areas. This review explores latest strategies intended support conservation, maximize improve utilization farming, highlighting critical roles that health management play farming. such as efficiency uptake through enhanced root systems increased transport mechanisms are well discussed. study suggests addressing potential obstacles, ethical regulatory concerns, a necessity for long-term sustainability applications technologies raise agricultural yields.

Language: Английский

Citations

1

Addressing the UN sustainable development goals of woody plants through genetic engineering DOI
Rafael Urrea‐López, Andrés Gatica-Arias

Plant Cell Tissue and Organ Culture (PCTOC), Journal Year: 2024, Volume and Issue: 158(3)

Published: Aug. 22, 2024

Language: Английский

Citations

1

Recent advances of Plant Growth Promoting Rhizobacteria (PGPR) for eco-restoration of polluted soil DOI Creative Commons
Muhammad Iqbal, Kharibam Karnajit Singh, Ram Chandra

et al.

Cleaner Engineering and Technology, Journal Year: 2024, Volume and Issue: unknown, P. 100845 - 100845

Published: Nov. 1, 2024

Language: Английский

Citations

1

Genetic modification – benefits and risks for New Zealand grassland production systems DOI Creative Commons
J. R. Caradus

Journal of New Zealand Grasslands, Journal Year: 2023, Volume and Issue: unknown, P. 39 - 49

Published: Nov. 10, 2023

Fundamentally plant breeding is about expanding and exploiting the genetic potential of plants (Stoskopf et al. 2019). Over time this has included amongst others phenotypic selection, mutagenesis, modification (GM) now gene editing (Bowerman 2023). In many crop species been a valuable option for delivering improved economic environmental outcomes. However, while extensively used in crops 25 years it rarely technology to advance trait expression forage grassland species. Worldwide, GM technologies have adopted at faster rate than any other recent (Raman 2017; Scheitrum 2020). Despite large government investment into use grass not outside containment New Zealand (Caradus 2008). The aim here review why so reluctant as another means providing solutions pastoral sector. Traits manipulated using range techniques that might application benefit systems will be reviewed, regulatory concerns need considered when adopting pasture discussed. Interestingly, 70 90% production globally animal feed (Flachowsky 2012; Ritchie Roser 2021). So if animals across world, including those USA, China Europe are being fed (Baulcombe 2014) would concerned plants? Although over decade old, useful summary views from variety stakeholders provided by Institute Agricultural & Horticultural Science (AgScience 2010).

Language: Английский

Citations

2

Epichloë fungal endophyte interactions in perennial ryegrass (Lolium perenne L.) modified to accumulate foliar lipids for increased energy density DOI Creative Commons

Kim Richardson,

Anouck C. M. de Bonth, Zac Beechey‐Gradwell

et al.

BMC Plant Biology, Journal Year: 2023, Volume and Issue: 23(1)

Published: Dec. 11, 2023

Abstract Background Commercial cultivars of perennial ryegrass infected with selected Epichloë fungal endophytes are highly desirable in certain pastures as the resulting mutualistic association has capacity to confer agronomic benefits (such invertebrate pest deterrence) largely due produced secondary metabolites (e.g., alkaloids). In this study, we investigated T 2 segregating populations derived from two independent transformation events expressing diacylglycerol acyltransferase (DGAT) and cysteine oleosin (CO) genes designed increase foliar lipid biomass accumulation. These were either festucae var . lolii strain AR1 or sp. LpTG-3 AR37 examine relationships between introduced trait endophytic association. Here report on experiments investigate if expression DGAT + CO tissues could negatively impact grass-endophyte vice versa Both endophyte plant characters measured under controlled environment field conditions. Results Expected relative increases total fatty acids 17–58% accrued a result no significant difference endophyte-infected non-infected progeny. Hyphal growth appeared normal when compared control plants chamber. There was mycelial for both strains AR37, however, -derived alkaloid concentrations significantly lower some occasions corresponding null-segregant progenies, although these remained within reported range bioactivity. Conclusions results suggest that formed does not influence host technology, but performance may be reduced circumstances. Further investigation will now required determine preferred genetic backgrounds introgression combination strains, grass genetics is major determinant success species.

Language: Английский

Citations

2

Rapid Identification of Insecticide- and Herbicide-Tolerant Genetically Modified Maize Using Mid-Infrared Spectroscopy DOI Open Access
Xiaodan Liu, Yonghui Yu, Xiulin Bai

et al.

Processes, Journal Year: 2022, Volume and Issue: 11(1), P. 90 - 90

Published: Dec. 29, 2022

Genetically modified (GM) technology is of great significance for increasing crop production, protecting biodiversity, and reducing environmental pollution. However, with the frequent occurrence safety events regarding GM foods, more disputes have arisen over potential transgenic technology. It particularly necessary to find a fast accurate method product identification. In this research, mid-infrared spectroscopy, coupled chemometric methods, was applied discriminate maize from its non-GM parent. A total 120 samples were prepared, spectral information in range 400–4000 cm−1 collected. After acquiring spectra, wavelet transform (WT) used preprocess data, k-means carried out split all into calibration prediction sets ratio 2:1. Principal component analysis (PCA) then conducted qualitatively distinguish two types samples, an apparent cluster observed. Since full spectrum covered large amount data redundant information, we adopted successive projections algorithm (SPA) select optimal wavelengths further analysis. Chemometrics, including partial least squares-discriminant (PLS-DA), k-nearest neighbor (KNN), extreme learning machine (ELM), performed establish classification models based on spectra wavelengths. The overall results indicated that ELM showed better accuracy reliability, 100% recognition rate set 98.75% set. has been confirmed combined can be novel approach identify maize.

Language: Английский

Citations

4