Catalase (CAT) Gene Family in Oil Palm (Elaeis guineensis Jacq.): Genome-Wide Identification, Analysis, and Expression Profile in Response to Abiotic Stress DOI Open Access
Lixia Zhou,

Jerome Jeyakumar John Martin,

Rui Li

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(3), P. 1480 - 1480

Published: Jan. 25, 2024

Catalases (CATs) play crucial roles in scavenging H2O2 from reactive oxygen species, controlling the growth and development of plants. So far, genome-wide identification characterization CAT genes oil palm have not been reported. In present study, five EgCAT were obtained through a approach. Phylogenetic analysis divided them into two subfamilies, with closer sharing similar structures. Gene structure conserved motif demonstrated nature intron/exon organization motifs among genes. Several cis-acting elements related to hormone, stress, defense responses identified promoter regions EgCATs. Tissue-specific expression different tissues was also revealed by heatmap using available transcriptome data. Stress-responsive showed that significantly expressed under cold, drought, salinity stress conditions. Collectively, this study provided valuable information on gene family validated can be used as potential candidates for improving abiotic tolerance other crops.

Language: Английский

MicroRNA: A Dynamic Player from Signalling to Abiotic Tolerance in Plants DOI Open Access
Ziming Ma,

Lanjuan Hu

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(14), P. 11364 - 11364

Published: July 12, 2023

MicroRNAs (miRNAs) are a class of non-coding single-stranded RNA molecules composed approximately 20–24 nucleotides in plants. They play an important regulatory role plant growth and development as signal abiotic tolerance. Some stresses include drought, salt, cold, high temperature, heavy metals nutritional elements. miRNAs affect gene expression by manipulating the cleavage, translational or DNA methylation target messenger RNAs (mRNAs). This review describes current progress field considering two aspects: (i) way produced regulated (ii) miRNA/target genes used responses to various stresses. Studying molecular mechanism action miRNAs’ downstream could optimize genetic manipulation crop conditions provide more theoretically optimized basis for improving production. MicroRNA is novel signalling interplant communication relating

Language: Английский

Citations

18
Epigenetic Modifications of Hormonal Signaling Pathways in Plant Drought Response and Tolerance for Sustainable Food Security DOI Open Access
Cengiz Kaya, Ferhat Uğurlar, Ioannis‐Dimosthenis S. Adamakis

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(15), P. 8229 - 8229

Published: July 28, 2024

Drought significantly challenges global food security, necessitating a comprehensive understanding of plant molecular responses for effective mitigation strategies. Epigenetic modifications, such as DNA methylation and histone are key in regulating genes hormones essential drought response. While microRNAs (miRNAs) primarily regulate gene expression post-transcriptionally, they can also interact with epigenetic pathways potential effectors that influence chromatin remodeling. Although the role miRNAs memory is still being explored, their contribution to response requires examining these indirect effects on modifications. A aspect this exploration drought-adapted plants, offering insights into transgenerational inheritance adaptive traits. Understanding mechanisms govern maintenance erasure imprints provides nuanced how plants balance stability flexibility epigenomes. major focus dynamic interaction between hormonal pathways—such those abscisic acid (ABA), ethylene, jasmonates, salicylic (SA)—and mechanisms. This interplay crucial fine-tuning during stress, leading physiological morphological adaptations enhance resilience. review highlights transformative advanced technologies, bisulfite sequencing CRISPR-Cas9, providing water deficit conditions. These technologies pave way developing drought-tolerant crops, which vital sustainable agriculture.

Language: Английский

Citations

6
Genome-wide identification of CCO gene family in cucumber (Cucumis sativus) and its comparative analysis with A. thaliana DOI Creative Commons

Jannat Akram,

Riffat Siddique,

Muhammad Shafiq

et al.

BMC Plant Biology, Journal Year: 2023, Volume and Issue: 23(1)

Published: Dec. 11, 2023

Abstract Carotenoid cleavage oxygenase (CCO) is an enzyme capable of converting carotenoids into volatile, aromatic compounds and it plays important role in the production two significant plant hormones, i.e., abscisic acid (ABA) strigolactone (SL). The cucumber genome has not been mined for genomewide identification CCO gene family. In present study, we conducted a comprehensive genome-wide analysis to identify thoroughly examine family within genomic sequence Cucumis sativus L. A Total 10 genes were identified mostly localized cytoplasm chloroplast. divided seven subfamilies i.e. 3 NCED, CCD, 1 CCD-like (CCDL) subfamily according phylogenetic analysis. Cis-regulatory elements (CREs) revealed associated with growth development as well reactions phytohormonal, biotic, abiotic stress conditions. CCOs involved variety physiological metabolic processes, Gene Ontology annotation. Additionally, regulated by 84 miRNA. CsCCO had substantial purifying selection acting upon them, synteny block. addition, RNAseq indicated that expressed response phloem transportation treatment chitosan oligosaccharides. CsCCD7 CsNCED2 showed highest expression exogenous application oligosaccharides improve cold cucumbers. We also found these CsCCD4a CsCCDL-a different organs respect content. was subject first report this which may help us better understand proteins lay groundwork family's future cloning functional investigations.

Language: Английский

Citations

11
Identifying long non-coding RNAs involved in heat stress response during wheat pollen development DOI Creative Commons
Saeid Babaei, Prem L. Bhalla, Mohan B. Singh

et al.

Frontiers in Plant Science, Journal Year: 2024, Volume and Issue: 15

Published: Feb. 6, 2024

Introduction Wheat is a staple food crop for over one-third of the global population. However, stability wheat productivity threatened by heat waves associated with climate change. Heat stress at reproductive stage can result in pollen sterility and failure grain development. Methods This study used transcriptome data analysis to explore specific expression long non-coding RNAs (lncRNAs) response during development four cultivars. Results discussion We identified 11,054 lncRNA-producing loci, which 5,482 lncRNAs showed differential stress. Heat-responsive could target protein-coding genes cis trans lncRNA-miRNA-mRNA regulatory networks. Gene ontology predicted that regulate various biological processes such as hormonal responses, protein modification folding, stress, biosynthetic metabolic processes. also noted some paired lncRNA/protein-coding gene modules shared two or more These were related regulating plant responses heat-shock proteins transcription factors, domains, MADS-box, Myc-type, Alpha crystallin/Hsp20 domain. Conclusion Our results provide basic knowledge molecular resources future functional studies investigating under

Language: Английский

Citations

4
An efficient CRISPR‐Cas12a‐mediated MicroRNA knockout strategy in plants DOI Creative Commons
Xuelian Zheng, Xu Tang, Yuechao Wu

et al.

Plant Biotechnology Journal, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 14, 2024

Summary In recent years, the CRISPR‐Cas9 nuclease has been used to knock out MicroRNA (miRNA) genes in plants, greatly promoting study of miRNA function. However, due its propensity for generating small insertions and deletions, Cas9 is not well‐suited achieving a complete knockout genes. By contrast, CRISPR‐Cas12a generates larger which could significantly disrupt secondary structure pre‐miRNA prevent production mature miRNAs. Through case OsMIR390 rice, we confirmed that Cas12a more efficient tool than mutants gene. To further demonstrate CRISPR‐Cas12a‐mediated targeted nine OsMIRNA have different spaciotemporal expression previously investigated via genetic approaches. With CRISPR‐Cas12a, up 100% genome editing efficiency was observed at these loci. The resulting deletions suggest robustly generated null alleles Transcriptome profiling mutants, as well phenotypic analysis rice grains revealed function miRNAs controlling gene regulating grain quality seed development. This established an plants.

Language: Английский

Citations

4
Understanding the impacts of drought on peanuts (Arachis hypogaea L.): exploring physio-genetic mechanisms to develop drought-resilient peanut cultivars DOI Creative Commons
Sameer Pokhrel, Prasanna Kharel, Swikriti Pandey

et al.

Frontiers in Genetics, Journal Year: 2025, Volume and Issue: 15

Published: Jan. 8, 2025

Peanut is a vital source of protein, particularly in the tropical regions Asian and African countries. About three-quarters peanut production occurs worldwide arid semi-arid regions, making drought an important concern production. In US about two-thirds peanuts are grown non-irrigated lands, where accounts for 50 million USD loss each year. The looming threat climate change exacerbates this situation by increasing erratic rainfall. Drought not only reduces yield but also degrades product quality. Peanuts under stress exhibit higher levels pre-harvest aflatoxin contamination, toxic fungal metabolite detrimental to both humans animals. One way sustain drought-prone address contamination developing drought-tolerant cultivars, process that can be accelerated understanding underlying physiological genetic mechanisms tolerance stress. Different attributes have been identified cultivars help them cope with advent precise studies, artificial intelligence, high-throughput phenotyping, bioinformatics, data science significantly improved studies peanuts. Yet, breeding often challenge as it complex trait affected environmental conditions. Besides technological advancements, success cultivar development relies on identification suitable germplasm conservation variation.

Language: Английский

Citations

0
Molecular Mechanisms of Cold Stress Response in Cotton: Transcriptional Reprogramming and Genetic Strategies for Tolerance DOI
Washu Dev, Fahmida Sultana, Hongge Li

et al.

Plant Science, Journal Year: 2025, Volume and Issue: unknown, P. 112390 - 112390

Published: Jan. 1, 2025

Language: Английский

Citations

0
Drought stress and the effectiveness of transcriptomics in identifying drought tolerance mechanisms in plants DOI
Walter Chitarra, Federico Vita, Fabiano Sillo

et al.

Elsevier eBooks, Journal Year: 2025, Volume and Issue: unknown, P. 389 - 402

Published: Jan. 1, 2025

Language: Английский

Citations

0
Exploring MicroRNA's role in enhancing lipid biosynthesis in microalgae for green energy production DOI
Siying Li,

Cai-Qin Cheng,

Dan Huang

et al.

Journal of the Taiwan Institute of Chemical Engineers, Journal Year: 2025, Volume and Issue: unknown, P. 105980 - 105980

Published: Jan. 1, 2025

Language: Английский

Citations

0
microRNA-Mediated Regulation of Drought Stress Responses in Potatoes: Implications for Climate Resilience DOI
Arslan Asim, Faisal Baig

Published: Jan. 1, 2025

Language: Английский

Citations

0