Harnessing the Potential of Exosomal miRNAs: A Breakthrough in Rheumatoid Arthritis Diagnosis and Monitoring DOI Creative Commons
Jianmin Gong, Xiaoshan Zhang, Adeel Khan

et al.

Research Square (Research Square), Journal Year: 2023, Volume and Issue: unknown

Published: Sept. 29, 2023

Abstract Background Rheumatoid arthritis (RA) is an autoimmune disorder characterized by the inflammation-induced joint damage. Timely diagnosis and treatment are crucial to prevent lasting disability. Here, we aim evaluate whether exosomal miRNAs could serve as promising biomarkers for expeditious RA effective therapy. Methods First, performed small RNA sequencing determine miRNA profiles of serum exosomes within a screening cohort comprising 18 untreated active patients, along with age gender-matched healthy controls. Subsequently, were then validated in training consisting 24 patients controls, using RT-qPCR methodology. Finally, selected subjected validation larger 108 103 The diagnostic efficacy was evaluated receiver operating characteristic (ROC) curve analysis. biological functions determined gene ontology (GO) Kyoto Encyclopedia Genes Genomes (KEGG) analyses. Results Our results first demonstrated noteworthy upregulation three specific (miR-885-5p, miR-6894-3p, miR-1268a) compared combination Anti-Cyclic Citrullinated Peptide Antibodies (ACPA) exhibited excellent accuracy, yielding area under (AUC) 0.963 (95% confidence interval = 0.941–0.984), sensitivity 87.96%, specificity 93.20%. Notably, miR-885-5p remarkable discriminatory capacity itself indistinguishing ACPA- negative from AUC 0.993 CI 0.978-1), 96.67%, 100%. Moreover, assessment therapeutic effectiveness, expression miR-1268a displayed significant reduction on 29th day MTX patients. This decline paralleled trends observed TJC28 (28-joint tender count), SJC28 swollen DAS28-CRP (Disease Activity Score 28-joint count C-Reactive Protein), all which indicative disease activity. predictive analysis indicated that target pivotal signaling molecules involved inflammatory pathways, thereby demonstrating modulation immune system. Conclusions In this study, have successfully potential miRNAs, particularly miR-885-5p, miR-6894-3p miR-1268a, hold early prediction time. These findings highlight importance realm monitoring.

Language: Английский

Identification of serum exosomal miRNA biomarkers for diagnosis of Rheumatoid arthritis DOI
Jianmin Gong, Xiaoshan Zhang, Adeel Khan

et al.

International Immunopharmacology, Journal Year: 2024, Volume and Issue: 129, P. 111604 - 111604

Published: Feb. 5, 2024

Language: Английский

Citations

9
Stem Cell Therapies in Canine Cardiology: Comparative Efficacy, Emerging Trends, and Clinical Integration DOI Creative Commons
Ahmed Farag, Hanan Hendawy, Mahmoud H. Emam

et al.

Biomolecules, Journal Year: 2025, Volume and Issue: 15(3), P. 371 - 371

Published: March 4, 2025

Cardiovascular diseases are a leading cause of morbidity and mortality in dogs, with limited options available for reversing myocardial damage. Stem cell therapies have shown significant potential cardiac repair, owing to their immunomodulatory, antifibrotic, regenerative properties. This review evaluates the therapeutic applications mesenchymal stem cells (MSCs) derived from bone marrow, adipose tissue, Wharton’s jelly focus on role canine cardiology immunoregulatory Preclinical studies highlighted efficacy enhancing function, reducing fibrosis, promoting angiogenesis. Various delivery methods, including intracoronary intramyocardial injections, assessed safety efficacy. Challenges such as low retention, differentiation efficiency, variability responses also discussed. Emerging strategies, genetic modifications combination therapies, aim enhance MSCs. Additionally, advances systems regulatory frameworks reviewed support clinical translation. comprehensive evaluation underscores revolutionize cardiovascular disease management while identifying critical areas future research integration.

Language: Английский

Citations

1
Exosome Revolution or Marketing Mirage? AI-Based Multi-domain Evaluation of Claims, Scientific Evidence, Transparency, Public Sentiment, and Media Narratives DOI
Eqram Rahman, Karim Sayed,

Parinitha Rao

et al.

Aesthetic Plastic Surgery, Journal Year: 2025, Volume and Issue: unknown

Published: March 7, 2025

Language: Английский

Citations

1
Advances in the Generation of Constructed Cardiac Tissue Derived from Induced Pluripotent Stem Cells for Disease Modeling and Therapeutic Discovery DOI Creative Commons
Truman J. Roland, Kunhua Song

Cells, Journal Year: 2024, Volume and Issue: 13(3), P. 250 - 250

Published: Jan. 29, 2024

The human heart lacks significant regenerative capacity; thus, the solution to failure (HF) remains organ donation, requiring surgery and immunosuppression. demand for constructed cardiac tissues (CCTs) model treat disease continues grow. Recent advances in induced pluripotent stem cell (iPSC) manipulation, CRISPR gene editing, 3D tissue culture have enabled a boom iPSC-derived CCTs (iPSC-CCTs) with diverse types architecture. Compared 2D-cultured cells, iPSC-CCTs better recapitulate biology, demonstrating potential advance modeling, drug discovery, medicine, though could benefit from methods faithfully mimic physiology electrophysiology. Here, we summarize future developments vascularization, immunization, maturation of study therapy.

Language: Английский

Citations

7
Cellular Senescence Contributes to the Dysfunction of Tight Junctions in Submandibular Glands of Aging Mice DOI Creative Commons
Zhuo Chen,

Qian‐Ying Mao,

Jie‐Yuan Zhang

et al.

Aging Cell, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 9, 2025

ABSTRACT The current mechanism by which aging reduces salivary secretion is unknown. This study investigates the of aging‐related submandibular (SMG) dysfunction and evaluates therapeutic potential dental pulp stem cell‐derived exosomes (DPSC‐exos). We found that stimulated flow rate was significantly reduced in naturally D‐galactose‐induced mice (D‐gal mice) compared to control mice. Acinar atrophy periductal fibrosis SMGs parotid glands (PGs) were observed D‐gal mice, whereas sublingual (SLGs) had no notable alterations. accumulation senescent cells SMGs, along with a decrease claudin‐3 (Cldn‐3) expression alterations distribution Cldn1 Cldn3. Additionally, after D‐gal‐induced senescence SMG‐C6 cells, there paracellular pathway permeability, Cldn3 occludin, changes Cldn1, 3, 4, 7. Furthermore, injecting DPSC‐exos into improved rate, acinar atrophy, decreased SA‐β‐gal activity. Our identified increased can cause disrupting tight junction molecules, injection ameliorates SMG secretory dysfunction. These findings may provide new clues novel targets for SMGs.

Language: Английский

Citations

0
Low‐intensity pulsed ultrasound enhances the osteogenic potential of PDLSCs‐derived extracellular vesicles through COMP/PI3K/AKT DOI Open Access
Mingli Xiang, Qiushuang Guo,

Yulin Liu

et al.

The FASEB Journal, Journal Year: 2025, Volume and Issue: 39(1)

Published: Jan. 10, 2025

Abstract The therapeutic potential of extracellular vesicles (EVs) in bone regeneration is noteworthy; however, their clinical application impeded by low yield and limited efficacy. This study investigated the effect low‐intensity pulsed ultrasound (LIPUS) on efficacy EVs derived from periodontal ligament stem cells (PDLSCs) preliminarily explored its mechanism. PDLSCs were cultured with osteogenic media stimulated or without LIPUS, then LIPUS‐stimulated (L‐EVs) isolated separately. We biological characteristics effects these two cell proliferation, migration, differentiation, vivo vitro, mechanism analyzing protein profiles. LIPUS significantly secretion PDLSCs‐EVs, L‐EVs exhibited stronger promoting thereby enhancing new formation. stimulation affected profile 42 proteins upregulated 4 downregulated when compared EVs. level cartilage oligomeric matrix (COMP) EVs, which enhanced EVs' ability via PI3K/AKT pathway. proposes that has as an optimization method for tissue regeneration.

Language: Английский

Citations

0
Cancer stem cell analysis and targeting DOI

Mishal Safdar,

Z. M. Amin,

Muneeb Ullah

et al.

Methods in cell biology, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Language: Английский

Citations

0
Therapeutic Potential of Exosomal miRNAs: New Insights and Future Directions DOI

Haohong Duan,

Seyedeh Helya Siadat,

Saade Abdalkareem Jasim

et al.

Journal of Biochemical and Molecular Toxicology, Journal Year: 2025, Volume and Issue: 39(5)

Published: April 24, 2025

ABSTRACT Modern advancements in medicine include developing targeted drug delivery systems the medical field, which are designed to unravel potential of therapeutic products and overcome barriers effectiveness current approaches. Various nanopolymer carrier have been introduced this regard, simple characteristics extracellular vesicles drawn special attention their application as an effective tool. Exosomes very similar transport a lipid‐biomembrane covering aqueous core. They also contain both hydrophilic lipophilic substances deliver cargo desired targets. These properties enable exosomes some limitations liposomes. can easily diffuse into body fluids remain bloodstream for long time, crossing physiological entering cells. Exosomes, large volume biomolecules, do not stimulate immune responses accumulate liver or lungs instead target tissues. Recent regenerative enabled scientists utilize extracted from mesenchymal stem cells (MSCs), possess significant abilities, treating various diseases. The contents these crucial diagnosis treatment, they influence disease progression. Numerous vitro studies confirmed safety, effectiveness, promise conditions such cancer, neurodegenerative disorders, cardiovascular issues, orthopedic ailments. This article explores MSC‐derived outlines essential procedures preparation.

Language: Английский

Citations

0
Astragalus polysaccharides augment BMSC homing via SDF-1/CXCR4 modulation: a novel approach to counteract peritoneal mesenchymal transformation and fibrosis DOI Creative Commons

Funing Wang,

Huibo Dai,

Zi‐Ren Zhou

et al.

BMC Complementary Medicine and Therapies, Journal Year: 2024, Volume and Issue: 24(1)

Published: May 24, 2024

Abstract Purpose This study aimed to evaluate the potential of astragalus polysaccharide (APS) pretreatment in enhancing homing and anti-peritoneal fibrosis capabilities bone marrow mesenchymal stromal cells (BMSCs) elucidate underlying mechanisms. Methods Forty male Sprague-Dawley rats were allocated into four groups: control, peritoneal dialysis fluid (PDF), PDF + BMSCs, APS BMSCs (APS-pre-treated BMSCs). A model was induced using PDF. Dil-labeled administered intravenously. Post-transplantation, BMSC peritoneum pathological alterations assessed. Stromal cell-derived factor-1 (SDF-1) levels quantified via enzyme-linked immunosorbent assay (ELISA), while CXCR4 expression determined PCR immunofluorescence. Additionally, a co-culture system involving mesothelial (PMCs) established Transwell setup examine vitro effects on migration therapeutic efficacy, with inhibitor AMD3100 deployed dissect role SDF-1/CXCR4 axis its downstream impacts. Results In vivo experiments confirmed that pre-treatment notably facilitated targeted tissue PDF-treated rats, thereby amplifying their impact. exposure markedly increased SDF-1 serum samples, which encouraged CXCR4-positive BMSCs. Inhibition through application diminished migration, consequently attenuating response mesenchyme-to-mesothelial transition (MMT). Furthermore, upregulated intensified activation axis’s pathways, partially reversed AMD3100-induced effects. Conclusion augments pathway by increasing action bolsters amplifies suppressive influence MMT, improving fibrosis.

Language: Английский

Citations

3
Extracellular matrix hydrogels with fibroblast growth factor 2 containing exosomes for reconstructing skin microstructures DOI Creative Commons
Zheng Zhou, Ziheng Bu, Shiqiang Wang

et al.

Journal of Nanobiotechnology, Journal Year: 2024, Volume and Issue: 22(1)

Published: July 26, 2024

Abstract Decellularized extracellular matrix hydrogel (ECM hydrogel), a natural material derived from normal tissue with unique biocompatibility properties, is widely used for repair. However, there are still problems such as poor biological activity and insufficient antimicrobial property. To overcome these drawbacks, fibroblast growth factor 2 (FGF 2) containing exosome (exo FGF ) was prepared to increase the activity. Furthermore, capacity of ECM optimised by using copper ions ligand-bonded cross-linking agent. The decellularized hydrogel, intricately cross-linked through ligand bonds loaded @ECM/Cu 2+ has demonstrated exceptional properties. In vitro, exo effectively promoted cell proliferation, migration, antioxidant inhibited bacterial growth. vivo, wound area rat treated hydrogels were significantly smaller than that other groups at Day 5 (45.24% ± 3.15%), 10 (92.20% 2.31%) 15 (95.22% 1.28%). Histological examination showed angiogenesis collagen deposition. Overall, this potential inhibit promote healing in variety clinical applications.

Language: Английский

Citations

3