Frontiers in Cellular and Infection Microbiology,
Journal Year:
2024,
Volume and Issue:
14
Published: Nov. 14, 2024
Introduction
The
aim
of
this
study
was
to
compare
the
detection
performance
mNGS
against
that
conventional
tests
(CT)
in
patients
suffering
from
infection
after
kidney
transplantation.
Methods
A
total
138
samples
85
transplant
with
acute
or
chronic
infections
were
simultaneously
analyzed
using
and
CT
July
2021
August
2023.
Results
Compared
CT,
demonstrated
a
higher
sensitivity
(95.96%
vs.
27.27%)
but
lower
specificity
(48.72%
84.62%)
pathogen
detection.
Moreover,
exhibited
significant
advantages
detecting
mixed
rare
infections.
pathogens
commonly
identified
severe
respiratory
syndrome
coronavirus
2
(SARS-CoV-2),
followed
by
Pneumocystis
jirovecii
Cytomegalovirus
(CMV).
guided
precise
clinical
diagnosis
89.13%
cases
assisted
altering
therapeutics
empirical
antibiotic
approaches
personalized
plans
56.10%
cases,
including
treatment
escalation
(40.65%),
initiation
(11.38%),
drug
adjustment
(3.25%),
de-escalation
(0.81%).
Discussion
Our
superior
its
value.
This
reflected
great
potential
as
complementary
technology
for
patients.
Clinical Microbiology Reviews,
Journal Year:
2024,
Volume and Issue:
37(2)
Published: April 11, 2024
SUMMARYFungal
infections
are
on
the
rise,
driven
by
a
growing
population
at
risk
and
climate
change.
Currently
available
antifungals
include
only
five
classes,
their
utility
efficacy
in
antifungal
treatment
limited
one
or
more
of
innate
acquired
resistance
some
fungi,
poor
penetration
into
"sequestered"
sites,
agent-specific
side
effect
which
require
frequent
patient
reassessment
monitoring.
Agents
with
novel
mechanisms,
favorable
pharmacokinetic
(PK)
profiles
including
good
oral
bioavailability,
fungicidal
mechanism(s)
urgently
needed.
Here,
we
provide
comprehensive
review
agents,
both
improved
known
mechanisms
actions
new
currently
clinical
development
for
treating
invasive
yeast,
mold
(filamentous
fungi),
Diagnostics,
Journal Year:
2024,
Volume and Issue:
14(5), P. 520 - 520
Published: Feb. 29, 2024
The
world
has
seen
a
tremendous
increase
in
the
number
of
fungal
infections
during
past
two
decades.
Recently,
World
Health
Organisation
released
pathogen
priority
list
for
infections,
signifying
importance
these
fields
research
and
public
health.
Microbiology
laboratories
demand
an
upgrade
diagnostic
system
to
keep
up
with
increased
burden
infections.
Diagnosis
using
conventional
techniques
always
faced
limitations
terms
specificity,
sensitivity,
turnaround
time.
Although
methods
are
core
pillars
diagnosis,
there
is
need
molecular
approaches.
Molecular
have
revolutionised
field
diagnostics.
diverse
array
techniques,
including
like
Polymerase
Chain
Reaction
(PCR),
emerged
as
cornerstone
transformed
diagnostics,
providing
powerful
tools
rapid
accurate
identification
pathogens.
As
technologies
continue
evolve,
their
integration
into
routine
clinical
practice
holds
promise
improving
patient
outcomes
through
timely
targeted
antifungal
interventions.
This
review
will
cover
approaches
involved
moving
from
basic
advanced-level
nucleic-acid-based
high
throughput
decreased
time
diagnosis
serious
Journal of Fungi,
Journal Year:
2024,
Volume and Issue:
10(7), P. 447 - 447
Published: June 26, 2024
Invasive
fungal
diseases
(IFDs)
comprise
a
growing
healthcare
burden,
especially
given
the
expanding
population
of
immunocompromised
hosts.
Early
diagnosis
IFDs
is
required
to
optimise
therapy
with
antifungals,
in
setting
rising
rates
antifungal
resistance.
Molecular
techniques
including
nucleic
acid
amplification
tests
and
whole
genome
sequencing
have
potential
offer
utility
overcoming
limitations
traditional
phenotypic
testing.
However,
standardisation
methodology
interpretations
these
assays
an
ongoing
undertaking.
The
targeted
Journal of Clinical Microbiology,
Journal Year:
2024,
Volume and Issue:
62(6)
Published: May 2, 2024
ABSTRACT
Invasive
mold
infections
(IMIs)
are
associated
with
high
morbidity,
particularly
in
immunocompromised
patients,
mortality
rates
between
40%
and
80%.
Early
initiation
of
appropriate
antifungal
therapy
can
substantially
improve
outcomes,
yet
early
diagnosis
remains
difficult
to
establish
often
requires
multidisciplinary
teams
evaluating
clinical
radiological
findings
plus
supportive
mycological
findings.
Universal
digital
high-resolution
melting
(U-dHRM)
analysis
may
enable
rapid
robust
diagnoses
IMI.
A
universal
fungal
assay
was
developed
for
U-dHRM
used
generate
a
database
melt
curve
signatures
19
clinically
relevant
pathogens.
machine
learning
algorithm
(ML)
trained
automatically
classify
these
pathogen
curves
detect
novel
curves.
Performance
assessed
on
73
bronchoalveolar
lavage
samples
from
patients
suspected
Novel
were
identified
by
micropipetting
reactions
Sanger
sequencing
amplicons.
achieved
97%
overall
organism
identification
accuracy
turnaround
time
~4
hrs.
detected
pathogenic
molds
(
Aspergillus
,
Mucorales
Lomentospora
Fusarium
)
73%
30
classified
as
IMI,
including
mixed
infections.
Specificity
optimized
requiring
the
number
sample
be
>
8
volume
1
mL,
which
resulted
100%
specificity
21
at-risk
without
showed
promise
separate
or
combination
diagnostic
approach
standard
tests.
U-dHRM’s
speed,
ability
simultaneously
identify
quantify
pathogens
polymicrobial
samples,
emerging
opportunistic
aid
treatment
decisions,
improving
patient
outcomes.
IMPORTANCE
Improvements
diagnostics
invasive
urgently
needed.
This
work
presents
new
molecular
detection
that
addresses
technical
workflow
challenges
provide
fast
detection,
identification,
quantification
could
inform
Expert Review of Anti-infective Therapy,
Journal Year:
2024,
Volume and Issue:
unknown
Published: Sept. 13, 2024
Invasive
fungal
infections
(IFI)
present
a
major
medical
challenge,
with
an
estimated
6.5
million
cases
annually,
resulting
in
3.8
deaths.
Pathogens
such
as
Aspergillus
spp.
Candida
Mucorales
Cryptococcus
and
other
fungi
species
contribute
to
these
infections,
posing
risks
immunocompromised
individuals.
Early
accurate
diagnosis
is
crucial
for
effective
treatment
better
patient
outcomes.
Microorganisms,
Journal Year:
2025,
Volume and Issue:
13(1), P. 207 - 207
Published: Jan. 18, 2025
Background:
Despite
kidney
transplantation
being
a
life-saving
procedure,
patients
experience
high
risk
of
developing
fungal
infections
(FIs),
with
an
increased
both
morbidity
and
mortality,
especially
during
the
first
year
after
transplant.
Methods:
We
herein
conducted
narrative
review
most
common
FIs
in
transplant
recipients
(KTRs),
focus
on
prevalence,
factors,
prevention
strategies.
Results:
The
pathogens
KTRs
include
Candida
species
(up
to
70%
overall
FIs),
Aspergillus
species,
Pneumocystis
jiroveci,
Cryptococcus
species.
Fungal
colonization,
diabetes
mellitus,
chronic
liver
disease,
malnutrition,
pre-existing
lung
conditions
should
all
be
acknowledged
as
possible
predisposing
factors.
mortality
rate
can
vary
from
25
50%
according
different
settings
types
FIs.
Preventive
strategies
are
critical
for
reducing
incidence
this
population.
These
antifungal
prophylaxis,
environmental
precautions,
infection
control
measures.
use
novel
tools
(such
PCR-based
molecular
assays
NGS)
rapid
accurate
diagnosis
may
play
important
role.
Conclusions:
Early
recognition,
appropriate
therapy,
preventive
essential
improving
graft
loss
fatal
outcomes
vulnerable
Future
research
is
needed
optimize
diagnostic
tools,
identify
agents,
develop
better
prophylactic
high-risk
recipients.
