Clinical Microbiology Reviews, Journal Year: 2024, Volume and Issue: 37(2)
Published: April 11, 2024
SUMMARYFungal infections are on the rise, driven by a growing population at risk and climate change. Currently available antifungals include only five classes, their utility efficacy in antifungal treatment limited one or more of innate acquired resistance some fungi, poor penetration into "sequestered" sites, agent-specific side effect which require frequent patient reassessment monitoring. Agents with novel mechanisms, favorable pharmacokinetic (PK) profiles including good oral bioavailability, fungicidal mechanism(s) urgently needed. Here, we provide comprehensive review agents, both improved known mechanisms actions new currently clinical development for treating invasive yeast, mold (filamentous fungi),
Language: Английский
Citations
34Epidemiology of Invasive Candidiasis
Clinical Epidemiology, Journal Year: 2024, Volume and Issue: Volume 16, P. 549 - 566
Published: Aug. 1, 2024
Invasive candidiasis (IC) is an increasingly prevalent, costly, and potentially fatal infection brought on by the opportunistic yeast,
Language: Английский
Citations
11Nucleic-Acid-Based Molecular Fungal Diagnostics: A Way to a Better Future
Diagnostics, Journal Year: 2024, Volume and Issue: 14(5), P. 520 - 520
Published: Feb. 29, 2024
The world has seen a tremendous increase in the number of fungal infections during past two decades. Recently, World Health Organisation released pathogen priority list for infections, signifying importance these fields research and public health. Microbiology laboratories demand an upgrade diagnostic system to keep up with increased burden infections. Diagnosis using conventional techniques always faced limitations terms specificity, sensitivity, turnaround time. Although methods are core pillars diagnosis, there is need molecular approaches. Molecular have revolutionised field diagnostics. diverse array techniques, including like Polymerase Chain Reaction (PCR), emerged as cornerstone transformed diagnostics, providing powerful tools rapid accurate identification pathogens. As technologies continue evolve, their integration into routine clinical practice holds promise improving patient outcomes through timely targeted antifungal interventions. This review will cover approaches involved moving from basic advanced-level nucleic-acid-based high throughput decreased time diagnosis serious
Language: Английский
Citations
10Molecular Diagnostics for Invasive Fungal Diseases: Current and Future Approaches
Journal of Fungi, Journal Year: 2024, Volume and Issue: 10(7), P. 447 - 447
Published: June 26, 2024
Invasive fungal diseases (IFDs) comprise a growing healthcare burden, especially given the expanding population of immunocompromised hosts. Early diagnosis IFDs is required to optimise therapy with antifungals, in setting rising rates antifungal resistance. Molecular techniques including nucleic acid amplification tests and whole genome sequencing have potential offer utility overcoming limitations traditional phenotypic testing. However, standardisation methodology interpretations these assays an ongoing undertaking. The targeted
Language: Английский
Citations
10Universal digital high-resolution melting for the detection of pulmonary mold infections
Journal of Clinical Microbiology, Journal Year: 2024, Volume and Issue: 62(6)
Published: May 2, 2024
ABSTRACT Invasive mold infections (IMIs) are associated with high morbidity, particularly in immunocompromised patients, mortality rates between 40% and 80%. Early initiation of appropriate antifungal therapy can substantially improve outcomes, yet early diagnosis remains difficult to establish often requires multidisciplinary teams evaluating clinical radiological findings plus supportive mycological findings. Universal digital high-resolution melting (U-dHRM) analysis may enable rapid robust diagnoses IMI. A universal fungal assay was developed for U-dHRM used generate a database melt curve signatures 19 clinically relevant pathogens. machine learning algorithm (ML) trained automatically classify these pathogen curves detect novel curves. Performance assessed on 73 bronchoalveolar lavage samples from patients suspected Novel were identified by micropipetting reactions Sanger sequencing amplicons. achieved 97% overall organism identification accuracy turnaround time ~4 hrs. detected pathogenic molds ( Aspergillus , Mucorales Lomentospora Fusarium ) 73% 30 classified as IMI, including mixed infections. Specificity optimized requiring the number sample be > 8 volume 1 mL, which resulted 100% specificity 21 at-risk without showed promise separate or combination diagnostic approach standard tests. U-dHRM’s speed, ability simultaneously identify quantify pathogens polymicrobial samples, emerging opportunistic aid treatment decisions, improving patient outcomes. IMPORTANCE Improvements diagnostics invasive urgently needed. This work presents new molecular detection that addresses technical workflow challenges provide fast detection, identification, quantification could inform
Language: Английский
Citations
9Diagnosing fungal infections in clinical practice: a narrative review
Expert Review of Anti-infective Therapy, Journal Year: 2024, Volume and Issue: unknown
Published: Sept. 13, 2024
Invasive fungal infections (IFI) present a major medical challenge, with an estimated 6.5 million cases annually, resulting in 3.8 deaths. Pathogens such as Aspergillus spp. Candida Mucorales Cryptococcus and other fungi species contribute to these infections, posing risks immunocompromised individuals. Early accurate diagnosis is crucial for effective treatment better patient outcomes.
Language: Английский
Citations
5Fungal Infections in Kidney Transplant Recipients: A Comprehensive Narrative Review
Microorganisms, Journal Year: 2025, Volume and Issue: 13(1), P. 207 - 207
Published: Jan. 18, 2025
Background: Despite kidney transplantation being a life-saving procedure, patients experience high risk of developing fungal infections (FIs), with an increased both morbidity and mortality, especially during the first year after transplant. Methods: We herein conducted narrative review most common FIs in transplant recipients (KTRs), focus on prevalence, factors, prevention strategies. Results: The pathogens KTRs include Candida species (up to 70% overall FIs), Aspergillus species, Pneumocystis jiroveci, Cryptococcus species. Fungal colonization, diabetes mellitus, chronic liver disease, malnutrition, pre-existing lung conditions should all be acknowledged as possible predisposing factors. mortality rate can vary from 25 50% according different settings types FIs. Preventive strategies are critical for reducing incidence this population. These antifungal prophylaxis, environmental precautions, infection control measures. use novel tools (such PCR-based molecular assays NGS) rapid accurate diagnosis may play important role. Conclusions: Early recognition, appropriate therapy, preventive essential improving graft loss fatal outcomes vulnerable Future research is needed optimize diagnostic tools, identify agents, develop better prophylactic high-risk recipients.
Language: Английский
Citations
0Revisting diagnostics: Early and accurate diagnosis of invasive fungal infections: A health economic view on investing in innovative diagnostics
Clinical Microbiology and Infection, Journal Year: 2025, Volume and Issue: unknown
Published: Jan. 1, 2025
Language: Английский
Citations
0Invasive cerebral aspergillosis in a pregnant woman: a rare case of intracranial giant granuloma
BMC Infectious Diseases, Journal Year: 2025, Volume and Issue: 25(1)
Published: April 8, 2025
Language: Английский
Citations
0Morphed aflaxotin concentration produced by Aspergillus flavus strain VKMN22 on maize grains inoculated on agar culture
Food Chemistry Molecular Sciences, Journal Year: 2024, Volume and Issue: 8, P. 100197 - 100197
Published: Feb. 27, 2024
This study identified and monitored the levels of aflatoxins (B
Language: Английский
Citations
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